Sequencing:Article Title: Diagnostic marker for interferon responsiveness in multiple sclerosis
Article Snippet: .. Aegera Therapeutics Inc. provided cDNAs, Taqman probes and Taqman primers for XAF-1 as given in the Table below: FORWARD PRIMER SEQUENCE Human XAF 1 (SEQ ID NO. 1) 5′-CTC GGT GTG CAG GAA CTG TAA A-3′ REVERSE PRIMER SEQUENCE (SEQ ID NO. 2) 5′-CAG GAA CCG CAG GCA GTA A-3′ PROBE SEQUENCE (SEQ ID NO. 3) 5′-(FAM)TCT GCC AAC TTC ACC CTC CAT GAG G(TAMRA)-3′ Total RNA isolated from PBMN and T cells were reverse transcribed to yield first-strand cDNA and amplified using the Taqman one-step EZ RT-PCR Core reagents kit (Applied Biosystems, Foster City, Calif., USA). ..
Isolation:Article Title: Diagnostic marker for interferon responsiveness in multiple sclerosis
Article Snippet: .. Aegera Therapeutics Inc. provided cDNAs, Taqman probes and Taqman primers for XAF-1 as given in the Table below: FORWARD PRIMER SEQUENCE Human XAF 1 (SEQ ID NO. 1) 5′-CTC GGT GTG CAG GAA CTG TAA A-3′ REVERSE PRIMER SEQUENCE (SEQ ID NO. 2) 5′-CAG GAA CCG CAG GCA GTA A-3′ PROBE SEQUENCE (SEQ ID NO. 3) 5′-(FAM)TCT GCC AAC TTC ACC CTC CAT GAG G(TAMRA)-3′ Total RNA isolated from PBMN and T cells were reverse transcribed to yield first-strand cDNA and amplified using the Taqman one-step EZ RT-PCR Core reagents kit (Applied Biosystems, Foster City, Calif., USA). ..
Reverse Transcription:Article Title: Diagnostic marker for interferon responsiveness in multiple sclerosis
Article Snippet: .. Aegera Therapeutics Inc. provided cDNAs, Taqman probes and Taqman primers for XAF-1 as given in the Table below: FORWARD PRIMER SEQUENCE Human XAF 1 (SEQ ID NO. 1) 5′-CTC GGT GTG CAG GAA CTG TAA A-3′ REVERSE PRIMER SEQUENCE (SEQ ID NO. 2) 5′-CAG GAA CCG CAG GCA GTA A-3′ PROBE SEQUENCE (SEQ ID NO. 3) 5′-(FAM)TCT GCC AAC TTC ACC CTC CAT GAG G(TAMRA)-3′ Total RNA isolated from PBMN and T cells were reverse transcribed to yield first-strand cDNA and amplified using the Taqman one-step EZ RT-PCR Core reagents kit (Applied Biosystems, Foster City, Calif., USA). ..
Amplification:Article Title: Diagnostic marker for interferon responsiveness in multiple sclerosis
Article Snippet: .. Aegera Therapeutics Inc. provided cDNAs, Taqman probes and Taqman primers for XAF-1 as given in the Table below: FORWARD PRIMER SEQUENCE Human XAF 1 (SEQ ID NO. 1) 5′-CTC GGT GTG CAG GAA CTG TAA A-3′ REVERSE PRIMER SEQUENCE (SEQ ID NO. 2) 5′-CAG GAA CCG CAG GCA GTA A-3′ PROBE SEQUENCE (SEQ ID NO. 3) 5′-(FAM)TCT GCC AAC TTC ACC CTC CAT GAG G(TAMRA)-3′ Total RNA isolated from PBMN and T cells were reverse transcribed to yield first-strand cDNA and amplified using the Taqman one-step EZ RT-PCR Core reagents kit (Applied Biosystems, Foster City, Calif., USA). ..
Reverse Transcription Polymerase Chain Reaction:Article Title: Diagnostic marker for interferon responsiveness in multiple sclerosis
Article Snippet: .. Aegera Therapeutics Inc. provided cDNAs, Taqman probes and Taqman primers for XAF-1 as given in the Table below: FORWARD PRIMER SEQUENCE Human XAF 1 (SEQ ID NO. 1) 5′-CTC GGT GTG CAG GAA CTG TAA A-3′ REVERSE PRIMER SEQUENCE (SEQ ID NO. 2) 5′-CAG GAA CCG CAG GCA GTA A-3′ PROBE SEQUENCE (SEQ ID NO. 3) 5′-(FAM)TCT GCC AAC TTC ACC CTC CAT GAG G(TAMRA)-3′ Total RNA isolated from PBMN and T cells were reverse transcribed to yield first-strand cDNA and amplified using the Taqman one-step EZ RT-PCR Core reagents kit (Applied Biosystems, Foster City, Calif., USA). ..
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