long-read cdna sequencing (Oxford Nanopore)
Structured Review

Long Read Cdna Sequencing, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+sequence/cdna+sequencing/pmc07565673-8-5-2
Average 90 stars, based on 1 article reviews
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1) Product Images from "Deletion in the Bardet–Biedl Syndrome Gene TTC8 Results in a Syndromic Retinal Degeneration in Dogs"
Article Title: Deletion in the Bardet–Biedl Syndrome Gene TTC8 Results in a Syndromic Retinal Degeneration in Dogs
Journal: Genes
doi: 10.3390/genes11091090
Figure Legend Snippet: Full-length cDNA sequencing (ONT).
Techniques Used: Sequencing, Produced
Figure Legend Snippet: Expression of retinal marker genes. ( a ) The average expression of marker genes for each retinal cell type in the unaffected BE02 ( a ) and GS01 ( b ) dogs as well as the affected GR02 ( c ), based on cDNA sequencing. ( d ) Relative mRNA expression levels by quantitative RT-qPCR in two different regions (exons 7–8 and exons 13–14) of the TTC8 gene, as well as the retinal marker genes GFAP (macroglial cells), OPN1LW and OPN1SW (cone photoreceptors), RHO (rod photoreceptors), RPE65 (RPE cells), and POU4F1 (retinal ganglion cells) expression in three unaffected dogs (BE02, LR02, GS01) and the affected GR02, normalized to GAPDH and ACTB gene expression. Rod: rod photoreceptors, cone: cone photoreceptors, RGC: retinal ganglion cells, AC: amacrine cells, HC: horizontal cells, BC: bipolar cells, MG: macroglial cells, RPE: retinal pigment epithelium, BE02: unaffected beagle, GS01: unaffected German shepherd, GR02: affected golden retriever, LR02: unaffected Labrador retriever.
Techniques Used: Expressing, Marker, Sequencing, Quantitative RT-PCR, Gene Expression
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