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cdna samples  (fluidigm)


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    Structured Review

    fluidigm cdna samples
    Cdna Samples, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+samples/Preamp+Master+Mix/pm41326455-108-6-8
    Average 94 stars, based on 30 article reviews
    cdna samples - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Localized Delivery of a Small Molecule Hedgehog Agonist via Poly(ε-caprolactone) Scaffolds Enhances Tendon-to-Bone Integration.
    Article Snippet: The collected tissue from multiple samples was collated and then digested in Proteinase K (Zymo, D3001‐2‐5) digestion buffer (Zymo D3050‐1‐5) for 1 h at 55°C. .. RNA was isolated using the Quick‐RNA MicroPrep (Zymo, R1050) spin columns, converted to cDNA (Invitrogen SuperScript IV VILO), and amplified (Preamp master mix, Standard BioTools) for Gli1 (Mm00494654_m) as previously described [27]. .. Following this amplification step, qPCR for Gli1 was conducted and normalized to the 18S (Mm03928990_g1) housekeeping gene.

    Article Title: Localized Delivery of a Small Molecule Hedgehog Agonist via Poly(ε‐caprolactone) Scaffolds Enhances Tendon‐to‐Bone Integration
    Article Snippet: The collected tissue from multiple samples was collated and then digested in Proteinase K (Zymo, D3001‐2‐5) digestion buffer (Zymo D3050‐1‐5) for 1 h at 55°C. .. RNA was isolated using the Quick‐RNA MicroPrep (Zymo, R1050) spin columns, converted to cDNA (Invitrogen SuperScript IV VILO), and amplified (Preamp master mix, Standard BioTools) for Gli1 (Mm00494654_m) as previously described [ ]. .. Following this amplification step, qPCR for Gli1 was conducted and normalized to the 18S (Mm03928990_g1) housekeeping gene.

    Amplification:

    Article Title: Localized Delivery of a Small Molecule Hedgehog Agonist via Poly(ε-caprolactone) Scaffolds Enhances Tendon-to-Bone Integration.
    Article Snippet: The collected tissue from multiple samples was collated and then digested in Proteinase K (Zymo, D3001‐2‐5) digestion buffer (Zymo D3050‐1‐5) for 1 h at 55°C. .. RNA was isolated using the Quick‐RNA MicroPrep (Zymo, R1050) spin columns, converted to cDNA (Invitrogen SuperScript IV VILO), and amplified (Preamp master mix, Standard BioTools) for Gli1 (Mm00494654_m) as previously described [27]. .. Following this amplification step, qPCR for Gli1 was conducted and normalized to the 18S (Mm03928990_g1) housekeeping gene.

    Article Title: Localized Delivery of a Small Molecule Hedgehog Agonist via Poly(ε‐caprolactone) Scaffolds Enhances Tendon‐to‐Bone Integration
    Article Snippet: The collected tissue from multiple samples was collated and then digested in Proteinase K (Zymo, D3001‐2‐5) digestion buffer (Zymo D3050‐1‐5) for 1 h at 55°C. .. RNA was isolated using the Quick‐RNA MicroPrep (Zymo, R1050) spin columns, converted to cDNA (Invitrogen SuperScript IV VILO), and amplified (Preamp master mix, Standard BioTools) for Gli1 (Mm00494654_m) as previously described [ ]. .. Following this amplification step, qPCR for Gli1 was conducted and normalized to the 18S (Mm03928990_g1) housekeeping gene.

    Real-time Polymerase Chain Reaction:

    Article Title: Impact of feeding diets without mineral P supplement on the immune system of two laying hen strains.
    Article Snippet: .. With a pool of all primers used for the final qPCR runs, a pre-amplification step was performed using the Preamp Master Mix (#100-5744, Standard Biotools, South San Francisco, CA) and 1.25 μL cDNA with 15 cycles (for spleen) or 13 cycles (for CT). ..

    other:

    Article Title: Regional FDL tendon properties are regulated by biglycan in a collagen VI-independent manner
    Article Snippet: Dissected regions were digested with proteinase K, and RNA was extracted from these samples (Zymo Quick-RNA Microprep).

    Concentration Assay:

    Article Title: Environmental tracking of viral pathogens in surface waters around Paris using high-throughput PCR.
    Article Snippet: Reverse transcription (RT) reactions were performed using the SuperScript IV First-Strand Synthesis System (Invitrogen) according to the manufacturer's protocol with 11 μL of viral RNA and 2.5 μM random hexamers, in 20 μL reaction mixtures. .. Pre-amplification reactions were performed in a 5 μL reaction mixture containing 1 μL of PreAmp Master Mix (Standard BioTools), 1.25 μL of the 200 nM primers pool (final concentration of each primer: 60 nM), 1.25 μL of cDNA and 1.25 μL of PCR-grade water. ..

    Polymerase Chain Reaction:

    Article Title: Environmental tracking of viral pathogens in surface waters around Paris using high-throughput PCR.
    Article Snippet: Reverse transcription (RT) reactions were performed using the SuperScript IV First-Strand Synthesis System (Invitrogen) according to the manufacturer's protocol with 11 μL of viral RNA and 2.5 μM random hexamers, in 20 μL reaction mixtures. .. Pre-amplification reactions were performed in a 5 μL reaction mixture containing 1 μL of PreAmp Master Mix (Standard BioTools), 1.25 μL of the 200 nM primers pool (final concentration of each primer: 60 nM), 1.25 μL of cDNA and 1.25 μL of PCR-grade water. ..

    Reverse Transcription:

    Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila.
    Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools). .. Expression of the 44 selected immunorelevant target genes and four reference genes (Supplementary Table 1) was analyzed on the BioMark HD System (Standard BioTools).

    Purification:

    Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila.
    Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools). .. Expression of the 44 selected immunorelevant target genes and four reference genes (Supplementary Table 1) was analyzed on the BioMark HD System (Standard BioTools).

    Binding Assay:

    Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila.
    Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools). .. Expression of the 44 selected immunorelevant target genes and four reference genes (Supplementary Table 1) was analyzed on the BioMark HD System (Standard BioTools).



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