gene exp tnc hs01115665 m1 (Thermo Fisher)
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gene exp tnc hs01115665 m1
Gene Exp Tnc Hs01115665 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+microarray+high-throughput/Gene+Exp%2E+TNC%2C+Hs01115665_m1/pmc10081170-195-3-5
Average 99 stars, based on 1 article reviews
Gene Exp Tnc Hs01115665 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+microarray+high-throughput/Gene+Exp%2E+TNC%2C+Hs01115665_m1/pmc10081170-195-3-5
Average 99 stars, based on 1 article reviews
gene exp tnc hs01115665 m1 - by Bioz Stars,
2026-10
99/100 stars
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1) Product Images from "Distinct tumor architectures for metastatic colonization of the brain"
Article Title: Distinct tumor architectures for metastatic colonization of the brain
Journal: bioRxiv
doi: 10.1101/2023.01.27.525190
Figure Legend Snippet: Scoring of IHC-stained patient-derived samples.
Techniques Used:
Figure Legend Snippet: (A) Schematic of the workflow of isolating, culturing, and comparing the transcriptome of brain metastatic (BrM) derivatives to the parental breast cancer cell lines they were isolated from. (B) GSEA showing that, in comparison to corresponding parental cell lines, the ECM organization pathway is enriched in the brain metastatic derivatives of HCC1954 and MMTV-ErbB2 HER2BC cells and not in MDA231 TNBC cells. Color shades indicate BH-adjusted P values of normalized enrichment scores. (C) Z-scored expression of the matrisome genes TNC , COL4A1 , IL24 , and SEMA7A that are differentially upregulated in HCC1954 and MMTV-Erbb2 HER2BC brain metastatic derivatives (BrM) in reference to their corresponding parental (Par) cell lines. (D) Forest plots showing the hazard ratio of the expression of matrisome genes COL4A1 , CST6 , SEMA7A , TNC , IL24 , and S100A7A for relapse-free survival of HER2BC breast cancer patients. Gene names in red indicate core matrisome genes. P values from top to bottom are as follows: 0.0012, 0.0135, 0.0894, 0.0641, 0.1649, 0.0889. (E) Schematic of the MetMap workflow using barcoded cancer cell line pools for high-throughput metastatic potential profiling (adapted from Ref. ). Relative metastatic potential was quantified by deep sequencing of barcode abundance from tissue. Comparing the transcriptome of in vivo brain metastases to that of in vitro cell culture per multiplexed cell line pool yielded the log2 fold change (log2FC) of gene expression shown in (F). (F) Relative in vivo expression, visualized by the log2FC values shown in a descending order, of the top ECM component genes that are differentially upregulated in the brain metastasis samples composed predominantly of HER2BC cells (pink) than of TNBC cells (blue) (see for statistical analysis of the association between relative in vivo expression and percent of HER2BC cells across multiplexed brain metastasis samples). (G) GSEA showing that ECM-related pathways are enriched in multiplexed brain metastasis samples composed predominantly of HER2BC cells (denoted in pink in (F)) than of TNBC cells (in blue in (F)). Top four positively enriched gene sets are Gene Ontology (GO) terms of 1, collagen containing extracellular matrix (GO:0062023), 2, extracellular matrix (GO:0031012), 3, extracellular structure organization (GO:0043062), and 4, extracellular matrix structural constituent (GO:0005201).
Techniques Used: Isolation, Comparison, Expressing, High Throughput Screening Assay, Sequencing, In Vivo, In Vitro, Cell Culture, Gene Expression
Figure Legend Snippet: (A) Representative IF staining showing the TNC deposition in incipient spheroidal colonies formed by HCC1954-BrM cells 7 days post-intracardiac inoculation. Scale bar, 50 μm. (B) Quantification of the IF staining signal of tenascin C (TNC), fibronectin (FN), and periostin (POSTN) per GFP+ (tumor) unit area in HCC1954-BrM HER2BC and MDA231-BrM TNBC brain metastatic colonies. RFU, relative fluorescence unit. Mean ± SEM. (C) (Left panel) Quantification and (right panel) representative IHC staining and associated TNC level scores (corresponding H&E staining shown in ) of TNC in brain metastasis tissue samples derived from TNBC patients (n = 13) and HER2BC patients (n = 18). Scale bars, 200 μm.
Techniques Used: Staining, Fluorescence, Immunohistochemistry, Derivative Assay
Figure Legend Snippet: (A-B) Effect of the suppression of COL4A1 and TNC expression on oncosphere formation (A) by shRNA in HCC1954-BrM cells and (B) by CRISPRi (2 sgRNAS per target gene) in MMTV-Erbb2-BrM cells, measured by the size of colonies after 5 days of growth in vitro . n = 100–175 colonies/group. Mean ± SEM. (C-D) Effect of the suppression of COL4A1 and TNC expression on brain colonization (C) by shRNA in HCC1954-BrM cells (n = 5 mice/group, 4 weeks after intracardiac inoculation into athymic mice, 2 shRNAs per target gene) and (D) by CRISPRi in MMTV-Erbb2-BrM cells (n = 5–9 mice/group, 3 weeks post-intracardiac inoculation into FVB/NJ mice, 2 sgRNAs per target gene), both quantified by whole-body BLI. (E-G) (E, G) Quantification and (F) representative IF staining of brain metastatic colonies formed by HCC1954-BrM cells expressing either a control vector or shRNAs that deplete COL4A1 or TNC expression. n = 3 mice/group. Mean ± SEM. Unpaired t test. (G) The percent of vascular coopting cells and (E) the number of colonies were determined 1 and 3 weeks post-intracardiac inoculation, respectively. (H) Schematic illustrates distinctive modes of colonization and stromal interface, various cancer cell-intrinsic mediators of colonization, and the induction of distinct DAM responses during the initiation of brain colonization in TNBC and HER2BC. Copyright © 2023 Memorial Sloan Kettering.
Techniques Used: Expressing, shRNA, In Vitro, Staining, Control, Plasmid Preparation
Figure Legend Snippet:
Techniques Used: Blocking Assay, Virus, Derivative Assay, Recombinant, Modification, Saline, Electron Microscopy, cDNA Synthesis, Sample Prep, Microarray, Gene Expression, Synthesized, Plasmid Preparation, Software, Magnetic Beads
Figure Legend Snippet:
Techniques Used: shRNA, Plasmid Preparation, Selection, Marker, Retroviral
Figure Legend Snippet:
Techniques Used: Sequencing
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