full length myh9 cdna (Genecopoeia)
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Structured Review
Genecopoeia
full length myh9 cdna
Full Length Myh9 Cdna, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+microarray+high-throughput/pmc06400792-47-13-16?v=Genecopoeia
Average 94 stars, based on 2 article reviews
Full Length Myh9 Cdna, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+microarray+high-throughput/pmc06400792-47-13-16?v=Genecopoeia
Average 94 stars, based on 2 article reviews
full length myh9 cdna - by Bioz Stars,
2026-08
94/100 stars
Images
1) Product Images from "MYH9 Promotes Growth and Metastasis via Activation of MAPK/AKT Signaling in Colorectal Cancer"
Article Title: MYH9 Promotes Growth and Metastasis via Activation of MAPK/AKT Signaling in Colorectal Cancer
Journal: Journal of Cancer
doi: 10.7150/jca.27635
Figure Legend Snippet: MYH9 is upregulated in CRC tissues compared with normal mucosa. A, MYH9 expression in CRC samples and paired non-neoplastic tissues was detected by western blot. β-tubulin was selected as a control for normalization. B, MYH9 staining intensity defined into four groups as negative, weak, moderate and strong in clinical CRC samples and adjacent normal tissues by IHC. C, MYH9 expression in the tumors and normal samples was examined by IHC. Representative figures are shown. Scale bars represent 50 µm. N, normal tissues. T, tumor tissues. The raw blots/gels are presented in Supplementary Figure .
Techniques Used: Expressing, Western Blot, Control, Staining
Figure Legend Snippet: MYH9 is positively correlated with progression and poor survival in CRC. A, Kaplan-Meier survival curves and univariate analyses (log-rank) for CRC patients with low expression (< median, n=97) and high expression (≥median, n=99) of MYH9 in the testing cohorts derived from a published high-throughput microarray dataset (NCBI/GEO/GSE87211; n=203). B, Kaplan-Meier plot of overall survival of 100 CRC patients (log rank=10.390, P =0.001). C, Overall survival of 38 CRC patients with N1 and N2 stage (log rank=5.320, P =0.021). D, Overall survival of 62 CRC patients with N0 stage (log rank=3.174, P =0.075).
Techniques Used: Expressing, Derivative Assay, High Throughput Screening Assay, Microarray
Figure Legend Snippet: Endogenous overexpression of MYH9 accelerates CRC cell growth and metastasis in vitro and in vivo . A, MYH9 expression level in 6 CRC cell lines were explored by western blot (left panel) and quantified (right panel). B, MYH9 mRNA expression (left panel) and protein expression (right panel) in SW620 cells infected with lentivirus including human full-length MYH9 cDNA (LV-MYH9) and empty vector (LV-Con). C, CCK-8 (left panel) and plate colony formation assay (right panel) in indicated cells. D, Cell migration (left panel) and invasion assay (right panel) in indicated cells. E, Nude mice tumorigenicity assay using indicated cells injected into hind limb flank. Tumor size and weight were shown in the right. F. H&E and MYH9 along with Ki67 staining of the tumors collected from the nude mice. Scale bars represent 50 µm. Error bars represented mean ± SD. Three independent experiments were carried out for each assay. (*), P <0.05; (**), P <0.01. The raw blots/gels are presented in Supplementary Figure .
Techniques Used: Over Expression, In Vitro, In Vivo, Expressing, Western Blot, Infection, Plasmid Preparation, CCK-8 Assay, Colony Assay, Migration, Invasion Assay, Tumorigenicity Assay, Injection, Staining
Figure Legend Snippet: Silencing of MYH9 inhibits proliferation and migration of CRC cells in vitro and in vivo . A, Knockdown effectiveness of four siRNAs were tested by real-time PCR (left panel) and western blot (right panel). B, Knockdown effectiveness of lentivirus (LV-siMYH9), which was derived from siMYH9-4 was validated by western blot. C, CCK-8 assay in indicated cells. D, Colony formation, migration and invasion assays in indicated cells. E, In vivo tumor formation assay using nude mice with tumor size and weight in the right. F, Representative figures of the tumors developed in nude mice with H&E, MYH9 and Ki67 staining. Scale bars represent 50 µm. Error bars served as mean ± SD for three independent experiments. Each experiment was repeated for three times. (*), P <0.05; (**), P <0.01. The raw blots/gels are presented in Supplementary Figure .
Techniques Used: Migration, In Vitro, In Vivo, Knockdown, Real-time Polymerase Chain Reaction, Western Blot, Derivative Assay, CCK-8 Assay, Tube Formation Assay, Staining
Figure Legend Snippet: EMT is required for MYH9-mediated CRC cell aggressive phenotypes. A, Cell morphology under inverted microscope in indicated cells (scale bars represent 20 µm). B, Western blot of EMT markers and MMP9 in indicated cells. Immunosignals of different protein expression level were quantified by greyscale scanning software (Quantity one) and relative protein abundance was normalized by β-tubulin. C, Immunofluorescence of EMT markers ZO-1 and β-catenin in indicated cells (scale bars represent 10 µm). D, EMT markers ZO-1 and β-catenin expression in nude mice derived tumors with MYH9 overexpression and knockdown by IHC. Scale bars represent 20 µm. The raw blots/gels are presented in Supplementary Figure .
Techniques Used: Inverted Microscopy, Western Blot, Expressing, Software, Quantitative Proteomics, Immunofluorescence, Derivative Assay, Over Expression, Knockdown
Figure Legend Snippet: MYH9 induces EMT and promotes CRC progression through MAPK and AKT signaling . A, Western blot analysis of protein expression related to AKT/ERK signaling pathway. B, Western blot analysis of AKT/ERK signaling pathway and EMT markers in indicated cells treated with AKT inhibitor LY29004 and MEK inhibitor U0126. Immunosignals of different protein expression level were quantified by greyscale scanning software (Quantity one) and relative protein abundance was normalized by β-tubulin. C, Transwell assay in indicated cells. D, Cell morphology of indicated cells was observed under inverted microscope. Scale bars represent 50 µm. Error bars represented mean ± SD from three independent experiments. (*), P <0.05; (**), P <0.01. The raw blots/gels are presented in Supplementary Figure &6.
Techniques Used: Western Blot, Expressing, Software, Quantitative Proteomics, Transwell Assay, Inverted Microscopy