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human glass 12k cdna chip  (Johns Hopkins HealthCare)

 
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    Structured Review

    Johns Hopkins HealthCare human glass 12k cdna chip
    Expression of IL-6 in TSU-Pr1 cells. TSU-Pr1 cells stably transfected with pp32 anti-sense express higher levels of IL-6 message as compared to parental TSU-Pr1 cells and vector-only control by RT-PCR analysis, which validates the <t>cDNA</t> microarray analysis (see Figure 6).
    Human Glass 12k Cdna Chip, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+facility/human+proteome+microarray/pmc01602226-105-21-12
    Average 90 stars, based on 1 article reviews
    human glass 12k cdna chip - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "pp32 Reduction Induces Differentiation of TSU-Pr1 Cells"

    Article Title: pp32 Reduction Induces Differentiation of TSU-Pr1 Cells

    Journal:

    doi:

    Expression of IL-6 in TSU-Pr1 cells. TSU-Pr1 cells stably transfected with pp32 anti-sense express higher levels of IL-6 message as compared to parental TSU-Pr1 cells and vector-only control by RT-PCR analysis, which validates the cDNA microarray analysis (see Figure 6).
    Figure Legend Snippet: Expression of IL-6 in TSU-Pr1 cells. TSU-Pr1 cells stably transfected with pp32 anti-sense express higher levels of IL-6 message as compared to parental TSU-Pr1 cells and vector-only control by RT-PCR analysis, which validates the cDNA microarray analysis (see Figure 6).

    Techniques Used: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Control, Reverse Transcription Polymerase Chain Reaction, Microarray

    Related Articles

    Microarray:

    Article Title: Chemical proteomics accelerates the target discovery of natural products.
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    Article Title: Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.
    Article Snippet: .. The HuProt microarray was obtained from the Johns Hopkins Medical Institutions Protein Microarray Core (CDI Laboratories, Inc).The array experiment was performed by Wayen Biotechnologies (Shanghai, China) according to the following procedure. ..

    Article Title: E17241 as a Novel ABCA1 (ATP-Binding Cassette Transporter A1) Upregulator Ameliorates Atherosclerosis in Mice
    Article Snippet: .. Protein binding microarray chips comprised of ≈20 000 individual human GST (glutathione S-transferase)- and His-tagged full-length proteins were obtained from the Johns Hopkins Medical Institutions Protein Microarray Core (CDI Laboratories, Inc). .. Microarray proteomic analyses were performed according to the procedure detailed below, and the data were processed24 by Wayen Biotechnologies Inc (Shanghai, China).

    Article Title: Deciphering the autoreactome: Massively parallelized methods for autoantibody detection in humans.
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    Article Title: The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation.
    Article Snippet: .. Protein array screening of US31-binding proteins The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6tagged full-length proteins. .. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen).

    Article Title: Targeting HINT1 to improve synaptic plasticity: toward loganin as a new antidepressant strategy.
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    Protein Binding:

    Article Title: E17241 as a Novel ABCA1 (ATP-Binding Cassette Transporter A1) Upregulator Ameliorates Atherosclerosis in Mice
    Article Snippet: .. Protein binding microarray chips comprised of ≈20 000 individual human GST (glutathione S-transferase)- and His-tagged full-length proteins were obtained from the Johns Hopkins Medical Institutions Protein Microarray Core (CDI Laboratories, Inc). .. Microarray proteomic analyses were performed according to the procedure detailed below, and the data were processed24 by Wayen Biotechnologies Inc (Shanghai, China).

    Article Title: The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation.
    Article Snippet: .. Protein array screening of US31-binding proteins The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6tagged full-length proteins. .. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen).

    Diagnostic Assay:

    Article Title: Identification of Novel Biomarkers for Behcet Disease Diagnosis Using Human Proteome Microarray Approach
    Article Snippet: .. This study was approved by the Ethics Committee of Peking Union Medical College Hospital. table ft1 table-wrap mode="anchored" t5 caption a7 Diagnostic symptom Positive number/ total(positive rate) Other symptom Positive number/ total(positive rate) Oral ulcers 110/113(97.3%) Untreated new-onset BD 59/113(52.2%) Genital ulcers 81/113(71.7%) Arthritis 22/107(20.6%) Eye lesions 28/113(24.78%) Epididymitis 8/105(7.6%) Skin lesions 68/113(60.18%) Gastrointestinal lesions 23/109(21.1%) Positive pathergy test 31/73(42.47%) Central nervous symptoms 15/112(13.4%) Vascular lesions 21/105(20.0%) Cardiac lesions 7/101(6.9%) Respiratory lesions 2/104(1.9%) Open in a separate window caption a8 The Clinical symptoms information of the BD patients Construction and Quality Control Test of HuProt Arrays The HuProt arrays, comprised of∼20,000 unique full-length proteins were constructed in Dr. Zhu's laboratory at Johns Hopkins University School of Medicine. .. Briefly, human ORFs cloned into a yeast expression vector (pEGH-A) were induced to produce N-terminally tagged GST fusions in Saccharomyces cerevisiae under control of the galactose-inducible GAL1 promoter.

    Control:

    Article Title: Identification of Novel Biomarkers for Behcet Disease Diagnosis Using Human Proteome Microarray Approach
    Article Snippet: .. This study was approved by the Ethics Committee of Peking Union Medical College Hospital. table ft1 table-wrap mode="anchored" t5 caption a7 Diagnostic symptom Positive number/ total(positive rate) Other symptom Positive number/ total(positive rate) Oral ulcers 110/113(97.3%) Untreated new-onset BD 59/113(52.2%) Genital ulcers 81/113(71.7%) Arthritis 22/107(20.6%) Eye lesions 28/113(24.78%) Epididymitis 8/105(7.6%) Skin lesions 68/113(60.18%) Gastrointestinal lesions 23/109(21.1%) Positive pathergy test 31/73(42.47%) Central nervous symptoms 15/112(13.4%) Vascular lesions 21/105(20.0%) Cardiac lesions 7/101(6.9%) Respiratory lesions 2/104(1.9%) Open in a separate window caption a8 The Clinical symptoms information of the BD patients Construction and Quality Control Test of HuProt Arrays The HuProt arrays, comprised of∼20,000 unique full-length proteins were constructed in Dr. Zhu's laboratory at Johns Hopkins University School of Medicine. .. Briefly, human ORFs cloned into a yeast expression vector (pEGH-A) were induced to produce N-terminally tagged GST fusions in Saccharomyces cerevisiae under control of the galactose-inducible GAL1 promoter.

    Construct:

    Article Title: Identification of Novel Biomarkers for Behcet Disease Diagnosis Using Human Proteome Microarray Approach
    Article Snippet: .. This study was approved by the Ethics Committee of Peking Union Medical College Hospital. table ft1 table-wrap mode="anchored" t5 caption a7 Diagnostic symptom Positive number/ total(positive rate) Other symptom Positive number/ total(positive rate) Oral ulcers 110/113(97.3%) Untreated new-onset BD 59/113(52.2%) Genital ulcers 81/113(71.7%) Arthritis 22/107(20.6%) Eye lesions 28/113(24.78%) Epididymitis 8/105(7.6%) Skin lesions 68/113(60.18%) Gastrointestinal lesions 23/109(21.1%) Positive pathergy test 31/73(42.47%) Central nervous symptoms 15/112(13.4%) Vascular lesions 21/105(20.0%) Cardiac lesions 7/101(6.9%) Respiratory lesions 2/104(1.9%) Open in a separate window caption a8 The Clinical symptoms information of the BD patients Construction and Quality Control Test of HuProt Arrays The HuProt arrays, comprised of∼20,000 unique full-length proteins were constructed in Dr. Zhu's laboratory at Johns Hopkins University School of Medicine. .. Briefly, human ORFs cloned into a yeast expression vector (pEGH-A) were induced to produce N-terminally tagged GST fusions in Saccharomyces cerevisiae under control of the galactose-inducible GAL1 promoter.

    Protein Array:

    Article Title: The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation.
    Article Snippet: .. Protein array screening of US31-binding proteins The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6tagged full-length proteins. .. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen).

    Produced:

    Article Title: The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation.
    Article Snippet: .. Protein array screening of US31-binding proteins The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6tagged full-length proteins. .. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen).



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    cdna microarray facility  (AECOM International Development)
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    AECOM International Development cdna microarray facility
    Complementary-template reverse-transcription (CT-RT) of single-stranded DNA primers reverse-transcribed from FFPE-RNA. ( a ) RNA extracted from FFPE tissue is reverse-transcribed, the mRNA/DNA duplex is filtered on an YM-50 column and the DNA is single-stranded with RNase-H and column purified. The 5′-NB-Oligo-dA (24) -cT7-3′ (complementary to the T7 promoter) is annealed to the <t>FFPE-cDNA</t> primers. ( b ) Total RNA from universal human reference (UHR, Stratagene) is amplified using the Sense-Amp cRNA amplification kit from Genisphere to provide RNA with the same orientation as messenger RNA . ( c ) Single-stranded DNA primers are hybridized to their sense-RNA template between 70 and 42°C for 90 min. The hybridized products are reverse-transcribed by a process described as CT-RT. The restored FFPE-cDNAs are doubled stranded and transcribed in vitro using T7 polymerase. (See Supplementary Data for technical description of points 1 through 6.)
    Cdna Microarray Facility, supplied by AECOM International Development, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+facility/cdna+microarrays/pmc01976468-129-11-19
    Average 90 stars, based on 1 article reviews
    cdna microarray facility - by Bioz Stars, 2026-09
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    Image Search Results


    Complementary-template reverse-transcription (CT-RT) of single-stranded DNA primers reverse-transcribed from FFPE-RNA. ( a ) RNA extracted from FFPE tissue is reverse-transcribed, the mRNA/DNA duplex is filtered on an YM-50 column and the DNA is single-stranded with RNase-H and column purified. The 5′-NB-Oligo-dA (24) -cT7-3′ (complementary to the T7 promoter) is annealed to the FFPE-cDNA primers. ( b ) Total RNA from universal human reference (UHR, Stratagene) is amplified using the Sense-Amp cRNA amplification kit from Genisphere to provide RNA with the same orientation as messenger RNA . ( c ) Single-stranded DNA primers are hybridized to their sense-RNA template between 70 and 42°C for 90 min. The hybridized products are reverse-transcribed by a process described as CT-RT. The restored FFPE-cDNAs are doubled stranded and transcribed in vitro using T7 polymerase. (See Supplementary Data for technical description of points 1 through 6.)

    Journal: Nucleic Acids Research

    Article Title: Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)

    doi: 10.1093/nar/gkm510

    Figure Lengend Snippet: Complementary-template reverse-transcription (CT-RT) of single-stranded DNA primers reverse-transcribed from FFPE-RNA. ( a ) RNA extracted from FFPE tissue is reverse-transcribed, the mRNA/DNA duplex is filtered on an YM-50 column and the DNA is single-stranded with RNase-H and column purified. The 5′-NB-Oligo-dA (24) -cT7-3′ (complementary to the T7 promoter) is annealed to the FFPE-cDNA primers. ( b ) Total RNA from universal human reference (UHR, Stratagene) is amplified using the Sense-Amp cRNA amplification kit from Genisphere to provide RNA with the same orientation as messenger RNA . ( c ) Single-stranded DNA primers are hybridized to their sense-RNA template between 70 and 42°C for 90 min. The hybridized products are reverse-transcribed by a process described as CT-RT. The restored FFPE-cDNAs are doubled stranded and transcribed in vitro using T7 polymerase. (See Supplementary Data for technical description of points 1 through 6.)

    Article Snippet: Arrays used for the studies were designed and printed at the cDNA Microarray Facility, Albert Einstein College of Medicine (AECOM), Bronx, NY.

    Techniques: Reverse Transcription, Purification, Amplification, In Vitro

    Experimental procedure utilized for the analysis of 10-year-old matched frozen and formalin-fixed paraffin embedded breast cancer samples. ( a ) Five micrograms of RNA extracted from the 10-year-old frozen portion of the sample, is reverse-transcribed and the cDNA is double stranded (dsDNA), in four individual reactions. The dsDNA of three reactions undergoes IVT-amplification (MessageAmpII, Ambion), which gives rise to complementary RNA (cRNA) for cDNA microarray analyses. The dsDNA of one reaction is used for PCR experiments. ( b ) Five micrograms of RNA extracted from the 10-year-old FFPE portion of the sample underwent the exact same process. ( c ) Single-stranded DNA (ssDNA) obtained by RT of 5 μg of FFPE-RNA is purified and hybridized to the sense-RNA template library. The restored ssDNA is double stranded and purified. Three of the CT-RT reactions undergo IVT-amplification, while the dsDNA of one reaction is used for PCR experiments.

    Journal: Nucleic Acids Research

    Article Title: Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)

    doi: 10.1093/nar/gkm510

    Figure Lengend Snippet: Experimental procedure utilized for the analysis of 10-year-old matched frozen and formalin-fixed paraffin embedded breast cancer samples. ( a ) Five micrograms of RNA extracted from the 10-year-old frozen portion of the sample, is reverse-transcribed and the cDNA is double stranded (dsDNA), in four individual reactions. The dsDNA of three reactions undergoes IVT-amplification (MessageAmpII, Ambion), which gives rise to complementary RNA (cRNA) for cDNA microarray analyses. The dsDNA of one reaction is used for PCR experiments. ( b ) Five micrograms of RNA extracted from the 10-year-old FFPE portion of the sample underwent the exact same process. ( c ) Single-stranded DNA (ssDNA) obtained by RT of 5 μg of FFPE-RNA is purified and hybridized to the sense-RNA template library. The restored ssDNA is double stranded and purified. Three of the CT-RT reactions undergo IVT-amplification, while the dsDNA of one reaction is used for PCR experiments.

    Article Snippet: Arrays used for the studies were designed and printed at the cDNA Microarray Facility, Albert Einstein College of Medicine (AECOM), Bronx, NY.

    Techniques: Formalin-fixed Paraffin-Embedded, Reverse Transcription, Amplification, Microarray, Purification

    Signal intensity and heat-map analysis of the correlation between the log 2 ratios measured by cDNA microarrays. ( a ) Signal intensity of one sample grid in the red channel (Cy5) across all microarrays. Top three panels display the grids obtained from three repeats using cRNA from 10-year-old frozen RNA (Frozen-Amp 1–3). Three mid-panels show the signal of three repeats using cRNA obtained by restoration and IVT-amplification of RNA from 10-year-old FFPE tissue (FFPE-Restored 1–3). Three bottom panels display the signal of three repeats using cRNA obtained by direct IVT-amplification of RNA from 10-year-old FFPE tissue. ( b ) Heat map displaying the log 2 of expression ratios ranging between 0.5 and 2 for 1044 genes detected in frozen tissue on a 28 032 features cDNA microarray and represented in the UHR library. From left to right are displayed the ratios obtained by IVT-amplification of RNA from 10-year-old frozen tissue (Frozen-Amp 1–3), restoration and IVT-amplification of RNA from 10-year-old FFPE tissue (FFPE-Restored 1–3) and direct IVT-amplification of RNA from 10-year-old FFPE tissue (FFPE-Amp 1–3). Each column represents an individual hybridization and each line a different feature. Red and blue represent up-regulated and down-regulated genes, respectively.

    Journal: Nucleic Acids Research

    Article Title: Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)

    doi: 10.1093/nar/gkm510

    Figure Lengend Snippet: Signal intensity and heat-map analysis of the correlation between the log 2 ratios measured by cDNA microarrays. ( a ) Signal intensity of one sample grid in the red channel (Cy5) across all microarrays. Top three panels display the grids obtained from three repeats using cRNA from 10-year-old frozen RNA (Frozen-Amp 1–3). Three mid-panels show the signal of three repeats using cRNA obtained by restoration and IVT-amplification of RNA from 10-year-old FFPE tissue (FFPE-Restored 1–3). Three bottom panels display the signal of three repeats using cRNA obtained by direct IVT-amplification of RNA from 10-year-old FFPE tissue. ( b ) Heat map displaying the log 2 of expression ratios ranging between 0.5 and 2 for 1044 genes detected in frozen tissue on a 28 032 features cDNA microarray and represented in the UHR library. From left to right are displayed the ratios obtained by IVT-amplification of RNA from 10-year-old frozen tissue (Frozen-Amp 1–3), restoration and IVT-amplification of RNA from 10-year-old FFPE tissue (FFPE-Restored 1–3) and direct IVT-amplification of RNA from 10-year-old FFPE tissue (FFPE-Amp 1–3). Each column represents an individual hybridization and each line a different feature. Red and blue represent up-regulated and down-regulated genes, respectively.

    Article Snippet: Arrays used for the studies were designed and printed at the cDNA Microarray Facility, Albert Einstein College of Medicine (AECOM), Bronx, NY.

    Techniques: Amplification, Expressing, Microarray, Hybridization