cdna oligonucleotide microarray data analysis (Thermo Fisher)
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Cdna Oligonucleotide Microarray Data Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+microarray+analysis/pmc02771024-127-18-19
Average 90 stars, based on 1 article reviews
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Labeling:Article Title: Castanea crenata honey reduces influenza infection by activating the innate immune response Article Snippet: EMSA was performed to determine the DNA-binding activity of NF-κB in influenza-infected cells with a DIG-labeled oligonucleotide (NF-κB: 5′-AGT TGA GGG GAC TTT CCC AGG C-3′) using nonradioactive EMSA kit (Roche, Mannheim, Germany). .. The nuclear extracts and labeled Article Title: Castanea crenata honey reduces influenza infection by activating the innate immune response. Article Snippet: EMSA was performed to determine the DNA-binding activity of NF-kB in influenza-infected cells with a DIG-labeled oligonucleotide (NF-kB: 5′-AGT TGA GGG GAC TTT CCC AGG C-3′) using nonradioactive EMSA kit (Roche, Mannheim, Germany). .. The nuclear extracts and labeled Incubation:Article Title: Castanea crenata honey reduces influenza infection by activating the innate immune response Article Snippet: EMSA was performed to determine the DNA-binding activity of NF-κB in influenza-infected cells with a DIG-labeled oligonucleotide (NF-κB: 5′-AGT TGA GGG GAC TTT CCC AGG C-3′) using nonradioactive EMSA kit (Roche, Mannheim, Germany). .. The nuclear extracts and labeled Article Title: Castanea crenata honey reduces influenza infection by activating the innate immune response. Article Snippet: EMSA was performed to determine the DNA-binding activity of NF-kB in influenza-infected cells with a DIG-labeled oligonucleotide (NF-kB: 5′-AGT TGA GGG GAC TTT CCC AGG C-3′) using nonradioactive EMSA kit (Roche, Mannheim, Germany). .. The nuclear extracts and labeled Binding Assay:Article Title: Castanea crenata honey reduces influenza infection by activating the innate immune response Article Snippet: EMSA was performed to determine the DNA-binding activity of NF-κB in influenza-infected cells with a DIG-labeled oligonucleotide (NF-κB: 5′-AGT TGA GGG GAC TTT CCC AGG C-3′) using nonradioactive EMSA kit (Roche, Mannheim, Germany). .. The nuclear extracts and labeled Article Title: Castanea crenata honey reduces influenza infection by activating the innate immune response. Article Snippet: EMSA was performed to determine the DNA-binding activity of NF-kB in influenza-infected cells with a DIG-labeled oligonucleotide (NF-kB: 5′-AGT TGA GGG GAC TTT CCC AGG C-3′) using nonradioactive EMSA kit (Roche, Mannheim, Germany). .. The nuclear extracts and labeled Plasmid Preparation:Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents Article Snippet: Finally, the dephosphorylation reaction (50 μl) was performed by incubation of the pJET1.2_Fluc vector using FastAP Thermosensitive Alkaline Phosphatase (5 μl, 5 U, Thermo) in 10 × Fast AP buffer (Thermo) at 37°C. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents. Article Snippet: The resulting DNA insert was purified using a commercial DNA purification kit (Macherey-Nagel) according to the protocol. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Luciferase:Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents Article Snippet: Finally, the dephosphorylation reaction (50 μl) was performed by incubation of the pJET1.2_Fluc vector using FastAP Thermosensitive Alkaline Phosphatase (5 μl, 5 U, Thermo) in 10 × Fast AP buffer (Thermo) at 37°C. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents. Article Snippet: The resulting DNA insert was purified using a commercial DNA purification kit (Macherey-Nagel) according to the protocol. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Purification:Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents Article Snippet: Finally, the dephosphorylation reaction (50 μl) was performed by incubation of the pJET1.2_Fluc vector using FastAP Thermosensitive Alkaline Phosphatase (5 μl, 5 U, Thermo) in 10 × Fast AP buffer (Thermo) at 37°C. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents. Article Snippet: The resulting DNA insert was purified using a commercial DNA purification kit (Macherey-Nagel) according to the protocol. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × DNA Purification:Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents Article Snippet: Finally, the dephosphorylation reaction (50 μl) was performed by incubation of the pJET1.2_Fluc vector using FastAP Thermosensitive Alkaline Phosphatase (5 μl, 5 U, Thermo) in 10 × Fast AP buffer (Thermo) at 37°C. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents. Article Snippet: The resulting DNA insert was purified using a commercial DNA purification kit (Macherey-Nagel) according to the protocol. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × other:Article Title: BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin Article Snippet: The membranes were blocked with 5% skim milk (BD, 232100) for 1 h at room temperature and incubated with the indicated antibodies overnight at 4°C and detected with horseradish peroxidase-conjugated secondary antibodies. Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents Article Snippet: The mixture was transferred to 42°C (30 sec, heat shock) and cooled on ice for 2 min. Multiplex Assay:Article Title: Development of cost-effective and fast KIR genotyping by multiplex PCR-SSP. Article Snippet: .. These samples were then typed using the multiplex PCR-SSP method and a Luminex-based reverse sequence-specific Polymerase Chain Reaction:Article Title: Development of cost-effective and fast KIR genotyping by multiplex PCR-SSP. Article Snippet: .. These samples were then typed using the multiplex PCR-SSP method and a Luminex-based reverse sequence-specific Sequencing:Article Title: Development of cost-effective and fast KIR genotyping by multiplex PCR-SSP. Article Snippet: .. These samples were then typed using the multiplex PCR-SSP method and a Luminex-based reverse sequence-specific Transfection:Article Title: β-Defensin-1 Regulates Influenza Virus Infection in Human Bronchial Epithelial Cells through the STAT3 Signaling Pathway Article Snippet: .. HBEpC were transfected with miRNA inhibitor oligonucleotide or a mimic |


