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Incyte corporation cdna microarray analyses
Cdna Microarray Analyses, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Microarray:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Article Title: ASBMR 23nd annual meeting.
Article Snippet: .. In this study, cDNA microarray analysis was conducted by Incyte Genomic Systems on 8,734 gene accessions using mRNA from mouse femurs (free of marrow) of both the congenic and B6 strains to test two hypotheses: 1) microarray analysis will identify candidate genes for bone density; and 2) by comparing the gene expression profile outside of the QTL region between B6 and the congenic mice, we can better characterize the molecular phenotypic mechanisms that contribute to the high bone density QTL. ..

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Article Title: Differentially expressed genes in large granular lymphocyte leukemia
Article Snippet: .. Overexpression of a variety cytotoxic genes was observed in leukemic LGL utilizing cDNA microarray from Incyte Genomics (FIG. 1 and Table 4). .. To verify the identity of these overexpressed genes, clones containing cDNA fragments of the selected genes were obtained from Incyte Genomics and confirmed by sequencing.

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Article Title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
Article Snippet: After 48 h, the surviving cells were stained with the alamarBlue assay (treK Diagnostic systems, cleveland, oH, UsA) for 180 min at 37°c. the absorbances of the wells were measured using a plate reader (ArVoTM MX; perkin elmer Inc., Waltham, MA, UsA). cDNA microarray analysis. the procedure of cDnA microarray analysis was performed as described previously (18). .. Briefly, cDNA generation, hybridization, and data collection for cDnA microarray analyses were performed by the Incyte corporation (palo Alto, cA, UsA). ..

Gene Expression:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Article Title: ASBMR 23nd annual meeting.
Article Snippet: .. In this study, cDNA microarray analysis was conducted by Incyte Genomic Systems on 8,734 gene accessions using mRNA from mouse femurs (free of marrow) of both the congenic and B6 strains to test two hypotheses: 1) microarray analysis will identify candidate genes for bone density; and 2) by comparing the gene expression profile outside of the QTL region between B6 and the congenic mice, we can better characterize the molecular phenotypic mechanisms that contribute to the high bone density QTL. ..

Real-time Polymerase Chain Reaction:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Labeling:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Hybridization:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Article Title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
Article Snippet: After 48 h, the surviving cells were stained with the alamarBlue assay (treK Diagnostic systems, cleveland, oH, UsA) for 180 min at 37°c. the absorbances of the wells were measured using a plate reader (ArVoTM MX; perkin elmer Inc., Waltham, MA, UsA). cDNA microarray analysis. the procedure of cDnA microarray analysis was performed as described previously (18). .. Briefly, cDNA generation, hybridization, and data collection for cDnA microarray analyses were performed by the Incyte corporation (palo Alto, cA, UsA). ..

Clone Assay:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Amplification:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Over Expression:

Article Title: Differentially expressed genes in large granular lymphocyte leukemia
Article Snippet: .. Overexpression of a variety cytotoxic genes was observed in leukemic LGL utilizing cDNA microarray from Incyte Genomics (FIG. 1 and Table 4). .. To verify the identity of these overexpressed genes, clones containing cDNA fragments of the selected genes were obtained from Incyte Genomics and confirmed by sequencing.

other:

Article Title: Three microarray platforms: an analysis of their concordance in profiling gene expression
Article Snippet: Incyte cDNA arrays and Operon long oligonucleotide (70-mer) arrays were printed in house, and Affymetrix 25-mer HG U133A arrays were purchased from the manufacturer.

Article Title: Multifactorial regulation of E-cadherin expression: an integrative study
Article Snippet: We have previously described our processing and normalization of NCI-60 transcript expression data from pin-spotted cDNA arrays (Incyte, Inc., Palo Alto, CA) ( 27 , 28 ), Affymetrix Hu-6800 arrays (Affymetrix, Sunnyvale, CA) ( 29 ), Affymetrix HG-U95 arrays ( 30 ), and Affymetrix HG-U133 ( 30 ).

Biomarker Discovery:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Tandem Mass Spectroscopy:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Western Blot:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..



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Summary of gene expression in breast muscle of BJY and AA chickens determined by  microarray  analysis

Journal: BMC Genomics

Article Title: Identification of differentially expressed genes and pathways for intramuscular fat deposition in pectoralis major tissues of fast-and slow-growing chickens

doi: 10.1186/1471-2164-13-213

Figure Lengend Snippet: Summary of gene expression in breast muscle of BJY and AA chickens determined by microarray analysis

Article Snippet: Agilent cDNA microarray analyses were conducted to determine gene expression profiles of breast muscle sampled at different developmental stages of BJY and AA chickens.

Techniques: Expressing, Microarray

Comparison of  microarray  and q-PCR fold-changes for selected transcripts in breast muscle of BJY or AA chickens

Journal: BMC Genomics

Article Title: Identification of differentially expressed genes and pathways for intramuscular fat deposition in pectoralis major tissues of fast-and slow-growing chickens

doi: 10.1186/1471-2164-13-213

Figure Lengend Snippet: Comparison of microarray and q-PCR fold-changes for selected transcripts in breast muscle of BJY or AA chickens

Article Snippet: Agilent cDNA microarray analyses were conducted to determine gene expression profiles of breast muscle sampled at different developmental stages of BJY and AA chickens.

Techniques: Microarray

Technical validation of microarray results by q-PCR by correlation: (a) BJY and (b) AA chickens. The r value indicates Spearman’s Correlation between the two methods.

Journal: BMC Genomics

Article Title: Identification of differentially expressed genes and pathways for intramuscular fat deposition in pectoralis major tissues of fast-and slow-growing chickens

doi: 10.1186/1471-2164-13-213

Figure Lengend Snippet: Technical validation of microarray results by q-PCR by correlation: (a) BJY and (b) AA chickens. The r value indicates Spearman’s Correlation between the two methods.

Article Snippet: Agilent cDNA microarray analyses were conducted to determine gene expression profiles of breast muscle sampled at different developmental stages of BJY and AA chickens.

Techniques: Microarray