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protoscript ii first strand cdna synthesis kit  (New England Biolabs)


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    Structured Review

    New England Biolabs protoscript ii first strand cdna synthesis kit
    Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1857 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+kit/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pm42129231-419-14-21
    Average 99 stars, based on 1857 article reviews
    protoscript ii first strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Article Title: IGF-1Eb Isoform Immunoreactivity May Be Associated with Placental Dysfunction and Vascular Pathology in Fetal Growth Restriction (FGR).
    Article Snippet: .. Complementary DNA (cDNA) synthesis was carried out by reverse transcription using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA, USA; cat. no. E6560L) according to the manufacturer’s instructions. .. The quality and purity of the extracted total RNA were assessed spectrophotometrically using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) by measuring absorbance at 260 and 280 nm.

    cDNA Synthesis:

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Dietary α-ketoglutarate modulates lung adenocarcinoma growth and epigenetic remodeling in a sex-dependent fashion.
    Article Snippet: .. First-strand cDNA synthesis was performed using the NEB ProtoScript II First Strand cDNA Synthesis Kit (E6560) according to the manufacturer’s protocol. ..

    Article Title: Comparison of Human Costal-Derived Chondrocyte Donor Sites and Microtia-Derived Chondrocytes for Auricular Tissue Engineering.
    Article Snippet: RNA was quantified using the NanoDrop spectrophotometer (Thermo Scientific, USA), and only samples with an A260/A280 greater than 1.8 were used. .. cDNA was prepared from 1 μg of RNA via the ProScript II First Strand cDNA Synthesis Kit (New England Biolabs, USA) according to the manufacturer’s instructions. .. Real-time quantitative PCR was performed using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, USA) on the CFX Duet Real-Time PCR System (Bio-Rad, USA).

    Article Title: Exposure and Bioaccumulation of Mercury Impacted the Extent of Cellular Damage and Oxidative Stress in Local Chickens Around Ilesha Gold Mining Areas of Nigeria.
    Article Snippet: Mercury (Hg) is a wellknown global pollutant of environmental concern.. Uncontrolled mining activities represent an avenue for its release and possible environmental contamination.. Ilesha gold mining areas are characterized by extensive artisan and smallscale gold mining (ASGM) activities.

    Article Title: The NORAD -Pumilio regulatory axis in the evolution of inclusion body myositis
    Article Snippet: Total RNA was extracted from frozen muscle blocks (20-μm-thick sections, 20 sections per block) using Mini RNeasy Plus Universal Kit (Qiagen, 74134). .. cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). .. Primer sets (synthesized by IDT) for NORAD (human, F: AAGCTGCTCTCAACTCCACC, R: GGACGTATCGCTTCCAGAGG; mouse, F: AGACCTCATCCTGTGAACCT, R: CCTCTTGAA CATGGAACTGCTA), PUM1 (human, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG; mouse, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG); and PUM2 (human, F: TCGACCACTACCATTTGTCAG, R: TTCCTCCTCTCCAAGTCCAA; mouse, F: AGAGATATACCGACCACTACCG, R: CAAGCCAAACTCCAGGACAT).

    Article Title: Native pathogen-phage networks reveal multilayered defense and gut-active anti-phage immunity.
    Article Snippet: .. For RNA-based qRT-PCR, cDNA was generated from purified RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB). ..

    Article Title: A splicing factor-cohesin association links RNA processing to cohesin-dependent genome folding.
    Article Snippet: RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo). .. Firststrand cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560). .. Quantitative PCR was performed using FastStart Universal SYBR Green Master (ROX) (Roche, #04913914001) on a qTOWER3 G touch real-time PCR system (Analytik Jena).

    Article Title: IGF-1Eb Isoform Immunoreactivity May Be Associated with Placental Dysfunction and Vascular Pathology in Fetal Growth Restriction (FGR).
    Article Snippet: .. Complementary DNA (cDNA) synthesis was carried out by reverse transcription using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA, USA; cat. no. E6560L) according to the manufacturer’s instructions. .. The quality and purity of the extracted total RNA were assessed spectrophotometrically using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) by measuring absorbance at 260 and 280 nm.

    Real-time Polymerase Chain Reaction:

    Article Title: Chromatin modifiers KMT2D, cBAF, and p300 cooperatively facilitate de novo binding of transcription factors on enhancers
    Article Snippet: .. Total RNA was extracted using TRIzol (#15596018, Invitrogen) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (#E6560,NEB), followingmanufacturer’s instructions.QuantitativeRTPCR (qRT-PCR) was performed in triplicate with the Luna Universal qPCR Master Mix (#M3003, NEB) using QuantStudio 5 Real-Time PCR System (Applied Biosystems). ..

    Synthesized:

    Article Title: The NORAD -Pumilio regulatory axis in the evolution of inclusion body myositis
    Article Snippet: Total RNA was extracted from frozen muscle blocks (20-μm-thick sections, 20 sections per block) using Mini RNeasy Plus Universal Kit (Qiagen, 74134). .. cDNAs were synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). .. Primer sets (synthesized by IDT) for NORAD (human, F: AAGCTGCTCTCAACTCCACC, R: GGACGTATCGCTTCCAGAGG; mouse, F: AGACCTCATCCTGTGAACCT, R: CCTCTTGAA CATGGAACTGCTA), PUM1 (human, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG; mouse, F: CTTCAAGACACATGCAACGC, R: TTGTCGGAGTGAAAGGTAAGG); and PUM2 (human, F: TCGACCACTACCATTTGTCAG, R: TTCCTCCTCTCCAAGTCCAA; mouse, F: AGAGATATACCGACCACTACCG, R: CAAGCCAAACTCCAGGACAT).

    Article Title: A splicing factor-cohesin association links RNA processing to cohesin-dependent genome folding.
    Article Snippet: RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo). .. Firststrand cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560). .. Quantitative PCR was performed using FastStart Universal SYBR Green Master (ROX) (Roche, #04913914001) on a qTOWER3 G touch real-time PCR system (Analytik Jena).

    Generated:

    Article Title: Native pathogen-phage networks reveal multilayered defense and gut-active anti-phage immunity.
    Article Snippet: .. For RNA-based qRT-PCR, cDNA was generated from purified RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB). ..

    Purification:

    Article Title: Native pathogen-phage networks reveal multilayered defense and gut-active anti-phage immunity.
    Article Snippet: .. For RNA-based qRT-PCR, cDNA was generated from purified RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB). ..



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