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Eurofins sequencing the entire cdna insert
Sequencing The Entire Cdna Insert, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+inserts+sequenced/cdna+sequencing/pm37535446-194-17-19
Average 90 stars, based on 1 article reviews
sequencing the entire cdna insert - by Bioz Stars, 2026-09
90/100 stars

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other:

Article Title: Form of Supplemental Selenium Affects the Expression of mRNA Transcripts Encoding Selenoproteins, and Proteins Regulating Cholesterol Uptake, in the Corpus Luteum of Grazing Beef Cows.
Article Snippet: All cDNA products were validated via DNA sequencing for verification of targets at Eurofins MWG Operon LLC (Louisville, KY, USA), as previously described [21,54].

Article Title: Form of dietary selenium affects mRNA encoding cholesterol biosynthesis and immune response elements in the early luteal phase bovine corpus luteum.
Article Snippet: D ow nloaded from https://academ ic.oup.com /jas/article/100/7/skac135/6620782 by guest on 10 February 2024 All cDNA products were validated via DNA sequencing for verification of target at Eurofins MWG Operon LLC (Louisville, KY), as described previously (Cerny et al., 2016a; Jia et al., 2018).

Article Title: Form of Supplemental Selenium Affects the Expression of mRNA Transcripts Encoding Selenoproteins, and Proteins Regulating Cholesterol Uptake, in the Corpus Luteum of Grazing Beef Cows
Article Snippet: All cDNA products were validated via DNA sequencing for verification of targets at Eurofins MWG Operon LLC (Louisville, KY, USA), as previously described [ , ].

Clone Assay:

Article Title: A novel chemokine binding protein 1-like gene is vital for the blood pool development and engorgement of the hard tick Haemaphysalis longicornis.
Article Snippet: Tick saliva modulates host responses during a blood feeding process.. We identified a novel chemokine binding protein 1-like (HLCBP1-like) gene from the salivary glands of the Asian longhorned tick, Haemaphysalis longicornis.. The HLCBP1-like protein, lacking a well-defined conserved domain, showed structural similarity to evasin, a chemokine binding protein from the brown dog tick, Rhipicephalus sanguineus.

Mutagenesis:

Article Title: A conserved arginine with non-conserved function is a key determinant of agonist selectivity in α7 nicotinic ACh receptors.
Article Snippet: Mutations were introduced in the α7, α4 or β2 nAChR subunits using the Stratagene QuikChange Site-Directed Mutagenesis Kit (Cat. No. 210518; Agilent, UK). .. The presence of the mutation and the absence of unwanted mutations were confirmed by sequencing the entire cDNA insert (Eurofins, UK). ..

Article Title: Side Groups Convert the α7 Nicotinic Receptor Agonist Ether Quinuclidine into a Type I Positive Allosteric Modulator.
Article Snippet: The quinuclidine scaffold has been extensively used for the development of nicotinic acetylcholine receptor (nAChR) agonists, with hydrophobic substituents at position 3 of the quinuclidine framework providing selectivity for α7 nAChRs.. In this study, six new ligands (4−9) containing a 3-(pyridin-3yloxy)quinuclidine moiety (ether quinuclidine) were synthesized to gain a better understanding of the structural−functional properties of ether quinuclidines.. To evaluate the pharmacological activity of these ligands, two-electrode voltage-clamp and singlechannel recordings were performed.

Sequencing:

Article Title: A conserved arginine with non-conserved function is a key determinant of agonist selectivity in α7 nicotinic ACh receptors.
Article Snippet: Mutations were introduced in the α7, α4 or β2 nAChR subunits using the Stratagene QuikChange Site-Directed Mutagenesis Kit (Cat. No. 210518; Agilent, UK). .. The presence of the mutation and the absence of unwanted mutations were confirmed by sequencing the entire cDNA insert (Eurofins, UK). ..

Article Title: Antiviral immune response reveals host-specific virus infections in natural ant populations
Article Snippet: RNA yield and quality of the final pools were measured on a Bioanalyzer (Agilent) and a Qubit Fluorometer (Invitrogen). .. Pools were sent for cDNA library preparation and sequencing to Eurofins Genomics GmbH (Ebersberg, Germany). .. For each pool two libraries were sequenced: one library of ribosomal RNA depleted, random primed cDNA 150 bp paired-end on a HiSeq 2500 v3 in rapid run mode, and one library of PAGE size selected (19–30 bp) on a HiSeq 2000 v3 in an Illumina platform.

Article Title: Side Groups Convert the α7 Nicotinic Receptor Agonist Ether Quinuclidine into a Type I Positive Allosteric Modulator.
Article Snippet: The quinuclidine scaffold has been extensively used for the development of nicotinic acetylcholine receptor (nAChR) agonists, with hydrophobic substituents at position 3 of the quinuclidine framework providing selectivity for α7 nAChRs.. In this study, six new ligands (4−9) containing a 3-(pyridin-3yloxy)quinuclidine moiety (ether quinuclidine) were synthesized to gain a better understanding of the structural−functional properties of ether quinuclidines.. To evaluate the pharmacological activity of these ligands, two-electrode voltage-clamp and singlechannel recordings were performed.

cDNA Library Assay:

Article Title: Antiviral immune response reveals host-specific virus infections in natural ant populations
Article Snippet: RNA yield and quality of the final pools were measured on a Bioanalyzer (Agilent) and a Qubit Fluorometer (Invitrogen). .. Pools were sent for cDNA library preparation and sequencing to Eurofins Genomics GmbH (Ebersberg, Germany). .. For each pool two libraries were sequenced: one library of ribosomal RNA depleted, random primed cDNA 150 bp paired-end on a HiSeq 2500 v3 in rapid run mode, and one library of PAGE size selected (19–30 bp) on a HiSeq 2000 v3 in an Illumina platform.

Synthesized:

Article Title: Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport
Article Snippet: The secreted region of FV (residues 29-2224) was inserted into the phlsec-vector (Addgene, 72348) with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit. .. A codon-optimized cDNA fragment of the secreted region of human AAT was synthesized (Eurofins) and inserted into the phlsec-vector with the C-terminal HiBiT-Tag. .. Human embryonic kidney (HEK) 293T cells were purchased from ATCC (American Type Culture Collection, CRL-3216).



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