Virus:Article Title: Immune response to adeno-associated viral vectors in a human iPSC-derived microglia chimeric mouse model.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IBA1 antibody Wako 019–19741 anti-GFP antibody Aves GFP-1010 Goat anti-Rabbit IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 790 ThermoFisher Scientific A11369 Goat anti-Rabbit IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor Plus 647 ThermoFisher Scientific A32733 Goat anti-Chicken IgY (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ThermoFisher Scientific A32931 Goat Anti-Chicken IgY H&L (Alexa Fluor 647) Abcam ab150171 Ly-6G Fluidigm 3141008B Ly-6C Biolegend 128039 CD4 Fluidigm 3145002B F4/80 Fluidigm 3146008B CD11b Fluidigm 3148003B CD68 Biolegend 137002 CD3e Fluidigm 3152004B CD8a Fluidigm 3153012B TER-119 Fluidigm 3154005B CD11c Fluidigm 3162017B CD19 Fluidigm 3166015B CD335 Fluidigm 3167008B CD117 Fluidigm 3173004B CD127 Fluidigm 3175006B FceRIa Fluidigm 3176006B MHC II Fluidigm 3209006B CD25 biolegend 101913 Siglec-F biolegend 155502 CD45 Fluidigm Europe BV 3089005B RAGE Abcam ab228861 Tim-3 BioLegend 345019 TREM2 R&D Systems MAB17291 CD279 (PD-1) BioLegend 329941 M6PR Abcam ab236067 CD206 (MMR) BioLegend 141702 HLA-A,B,C Fluidigm Europe BV 3141010B TGFBR1 Abcam ab51871 GM-CSFR/CSF2R R&D Systems MAB706 CD83 Biolegend 305302 EMR1 Biorad MCA2674GA CD64 Fluidigm Europe BV 3146006B CD11c Fluidigm Europe BV 3147008B CD16 Fluidigm Europe BV 3148004B CD68 Biolegend 137002 (Continued on next page) e1 Cell Reports Methods 6, 101362, April 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER CD169/SIGLEC Biolegend 346002 CLEC7A (Dectin-1) Biolegend 355402 CD3e Fluidigm Europe BV 3152004B CD192 (CCR2) Fluidigm Europe BV 3153023B CD45 Fluidigm Europe BV 3154001B CD36 Fluidigm Europe BV 3155012B CD86 Fluidigm Europe BV 3156008B CD284 (TLR4) Fluidigm Europe BV 3158024B CD115/CSF1R R&D Systems MAB329 CD80 Fluidigm Europe BV 3161023B CD95 Fluidigm Europe BV 3162038B CD172a/b (SIRPa/b) Fluidigm Europe BV 3163017B CX3CR1 Fluidigm Europe BV 3164023B CD40 Fluidigm Europe BV 3165005B CD204/MSR1 biolegend 371902 CD11b Fluidigm Europe BV 3167011B CD206 (MMR) Fluidigm Europe BV 3168008B CD33 Fluidigm Europe BV 3169010B C3aR Biolegend 345802 CD68 Fluidigm Europe BV 3171011B CX3CR1 Fluidigm Europe BV 3172017B HLA-DR Fluidigm Europe BV 3173005B I-A/I-E (MHCII) Fluidigm Europe BV 3174003B CD14 Fluidigm Europe BV 3175015B CD282 (TLR2) Fluidigm Europe BV 3176021B CD274/PD-L1 (MIH1) Fluidigm Europe BV 3209014B Bacterial and virus strains AAV6-mAIF1-RFP Vector Biolabs Custom order AAV2-CAG-RFP Vector Biolabs Custom order AAV9-CAG-RFP Vector Biolabs Custom order Chemicals, peptides, and recombinant proteins Anti-adherence rinsing solution Stem Cell Technologies 07010 Gibco DPBS, no calcium, no magnesium ThermoFisher Scientific 14190250 Accutase solution Merck A6964 Matrigel Growth factor reduced, phenol red-free, *LDEV-free Corning 356231 Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane Merck L2020 Essential 8 Flex Medium Kit ThermoFisher Scientific A2858501 IMDM medium ThermoFisher Scientific 21980032 Ham’s F-12 Nutrient Mix ThermoFisher Scientific 11765054 Phenol-free DMEM/F12 medium ThermoFisher Scientific 11039021 Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) ThermoFischer Scientific 51500056 Insulin-Transferrin-Selenium (ITS-G) ThermoFischer Scientific 41400045 L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate Merck A8960-5G 1-Thioglycerol Merck M-1753-100ML Poly(vinyl alcohol) Merck P8136-250G GlutaMAX Supplement ThermoFisher Scientific 35050061 (Continued on next page) Cell Reports Methods 6, 101362, April 20, 2026 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemically-defined lipid concentrate ThermoFisher Scientific 11905031 MEM Non-Essential Amino Acids Solution ThermoFisher Scientific 11140035 N2 supplements ThermoFisher Scientific 17502048 B27 supplements ThermoFisher Scientific 17504044 Insulin human Merck I2643-50mG Rho kinase inhibitor (Y-27632 dihydrochloride) Abcam ab120129 Recombinant Human IL-3 Peprotech 200–03 Recombinant Human IL-6 Peprotech 200–06 C-Kit Ligand (SCF) Recombinant Human Protein ThermoFisher Scientific PHC2115 Lithium chloride solution Merck L7026-100ML FGF-Basic (AA 1–155) Recombinant Human Protein ThermoFisher Scientific PHG0266 BMP4 Recombinant Human Protein ThermoFisher Scientific PHC9534 Activin A Recombinant Human Protein ThermoFisher Scientific PHC9564 Recombinant Human VEGF 165 Peprotech 100–20 Thrombopoietin (TPO) Recombinant Human Protein ThermoFisher Scientific PHC9514 Recombinant Human M-CSF PeproTech 300–25 Human TGF-β1 PeproTech 100–21 Recombinant Human IL-34 PeproTech 200–34 DMEM-F12 ThermoFisher Scientific 11320033 mTeSRTM Plus medium Stemcell Technologies 05825 CryoStor CS10 Stemcell Technologies 07959 pHrodo Red Zymosan Bioparticles Invitrogen P35364 Cytochalasin D Merck C8273-1MG Adenosine 5′-diphosphate sodium salt Sigma-Aldrich A2754-100MG PSB 0739 Tocris 3983 liposaccharide (LPS) Sigma L5024-10MG Heparin Sigma H3393-50KU Pluroni F-68 Non-ionic Surfactant (100X) Thermofisher 24040032 ProLongTM Gold Antifade Mountant ThermoFisher P36930 DMSO ThermoFisher Scientific D12345 Normal Goat Serum Abcam ab7481 Triton X-100 VWR 437002A Paraformaldehyde, 4% in PBS alfa aesar J19943 Dibenzylether Sigma-Aldrich 108014-1KG Dichloromethane Carlroth 8424.4 Methanol, 2,5L VWR 20847307 eBioscience 1X RBC Lysis Buffer ThermoFisher Scientific 00-4333-57 Percoll PLUS Sigma E0414-1L Maxpar Cell Staining Buffer Fluidigm 201068 Pierce 16% Formaldehyde (w/v), Methanol-free ThermoFisher 28908 Maxpar Cell Acquisition Solution Fluidigm 201237 Maxpar EQ Four Element Calibration Beads Fluidigm 201078 autoMACS Running Buffer – MACS Separation Buffer Miltenyi Biotech 130-091-221 Actinomycin D Sigma A1410 Triptolide Sigma T3652 Anisomycin Sigma A9789 1X HBSS Thermofisher 14175053 (Continued on next page) e3 Cell Reports Methods 6, 101362, April 20, 2026 Article ll OPEN ACCESS
Recombinant:Article Title: Immune response to adeno-associated viral vectors in a human iPSC-derived microglia chimeric mouse model.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IBA1 antibody Wako 019–19741 anti-GFP antibody Aves GFP-1010 Goat anti-Rabbit IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 790 ThermoFisher Scientific A11369 Goat anti-Rabbit IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor Plus 647 ThermoFisher Scientific A32733 Goat anti-Chicken IgY (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ThermoFisher Scientific A32931 Goat Anti-Chicken IgY H&L (Alexa Fluor 647) Abcam ab150171 Ly-6G Fluidigm 3141008B Ly-6C Biolegend 128039 CD4 Fluidigm 3145002B F4/80 Fluidigm 3146008B CD11b Fluidigm 3148003B CD68 Biolegend 137002 CD3e Fluidigm 3152004B CD8a Fluidigm 3153012B TER-119 Fluidigm 3154005B CD11c Fluidigm 3162017B CD19 Fluidigm 3166015B CD335 Fluidigm 3167008B CD117 Fluidigm 3173004B CD127 Fluidigm 3175006B FceRIa Fluidigm 3176006B MHC II Fluidigm 3209006B CD25 biolegend 101913 Siglec-F biolegend 155502 CD45 Fluidigm Europe BV 3089005B RAGE Abcam ab228861 Tim-3 BioLegend 345019 TREM2 R&D Systems MAB17291 CD279 (PD-1) BioLegend 329941 M6PR Abcam ab236067 CD206 (MMR) BioLegend 141702 HLA-A,B,C Fluidigm Europe BV 3141010B TGFBR1 Abcam ab51871 GM-CSFR/CSF2R R&D Systems MAB706 CD83 Biolegend 305302 EMR1 Biorad MCA2674GA CD64 Fluidigm Europe BV 3146006B CD11c Fluidigm Europe BV 3147008B CD16 Fluidigm Europe BV 3148004B CD68 Biolegend 137002 (Continued on next page) e1 Cell Reports Methods 6, 101362, April 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER CD169/SIGLEC Biolegend 346002 CLEC7A (Dectin-1) Biolegend 355402 CD3e Fluidigm Europe BV 3152004B CD192 (CCR2) Fluidigm Europe BV 3153023B CD45 Fluidigm Europe BV 3154001B CD36 Fluidigm Europe BV 3155012B CD86 Fluidigm Europe BV 3156008B CD284 (TLR4) Fluidigm Europe BV 3158024B CD115/CSF1R R&D Systems MAB329 CD80 Fluidigm Europe BV 3161023B CD95 Fluidigm Europe BV 3162038B CD172a/b (SIRPa/b) Fluidigm Europe BV 3163017B CX3CR1 Fluidigm Europe BV 3164023B CD40 Fluidigm Europe BV 3165005B CD204/MSR1 biolegend 371902 CD11b Fluidigm Europe BV 3167011B CD206 (MMR) Fluidigm Europe BV 3168008B CD33 Fluidigm Europe BV 3169010B C3aR Biolegend 345802 CD68 Fluidigm Europe BV 3171011B CX3CR1 Fluidigm Europe BV 3172017B HLA-DR Fluidigm Europe BV 3173005B I-A/I-E (MHCII) Fluidigm Europe BV 3174003B CD14 Fluidigm Europe BV 3175015B CD282 (TLR2) Fluidigm Europe BV 3176021B CD274/PD-L1 (MIH1) Fluidigm Europe BV 3209014B Bacterial and virus strains AAV6-mAIF1-RFP Vector Biolabs Custom order AAV2-CAG-RFP Vector Biolabs Custom order AAV9-CAG-RFP Vector Biolabs Custom order Chemicals, peptides, and recombinant proteins Anti-adherence rinsing solution Stem Cell Technologies 07010 Gibco DPBS, no calcium, no magnesium ThermoFisher Scientific 14190250 Accutase solution Merck A6964 Matrigel Growth factor reduced, phenol red-free, *LDEV-free Corning 356231 Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane Merck L2020 Essential 8 Flex Medium Kit ThermoFisher Scientific A2858501 IMDM medium ThermoFisher Scientific 21980032 Ham’s F-12 Nutrient Mix ThermoFisher Scientific 11765054 Phenol-free DMEM/F12 medium ThermoFisher Scientific 11039021 Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) ThermoFischer Scientific 51500056 Insulin-Transferrin-Selenium (ITS-G) ThermoFischer Scientific 41400045 L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate Merck A8960-5G 1-Thioglycerol Merck M-1753-100ML Poly(vinyl alcohol) Merck P8136-250G GlutaMAX Supplement ThermoFisher Scientific 35050061 (Continued on next page) Cell Reports Methods 6, 101362, April 20, 2026 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemically-defined lipid concentrate ThermoFisher Scientific 11905031 MEM Non-Essential Amino Acids Solution ThermoFisher Scientific 11140035 N2 supplements ThermoFisher Scientific 17502048 B27 supplements ThermoFisher Scientific 17504044 Insulin human Merck I2643-50mG Rho kinase inhibitor (Y-27632 dihydrochloride) Abcam ab120129 Recombinant Human IL-3 Peprotech 200–03 Recombinant Human IL-6 Peprotech 200–06 C-Kit Ligand (SCF) Recombinant Human Protein ThermoFisher Scientific PHC2115 Lithium chloride solution Merck L7026-100ML FGF-Basic (AA 1–155) Recombinant Human Protein ThermoFisher Scientific PHG0266 BMP4 Recombinant Human Protein ThermoFisher Scientific PHC9534 Activin A Recombinant Human Protein ThermoFisher Scientific PHC9564 Recombinant Human VEGF 165 Peprotech 100–20 Thrombopoietin (TPO) Recombinant Human Protein ThermoFisher Scientific PHC9514 Recombinant Human M-CSF PeproTech 300–25 Human TGF-β1 PeproTech 100–21 Recombinant Human IL-34 PeproTech 200–34 DMEM-F12 ThermoFisher Scientific 11320033 mTeSRTM Plus medium Stemcell Technologies 05825 CryoStor CS10 Stemcell Technologies 07959 pHrodo Red Zymosan Bioparticles Invitrogen P35364 Cytochalasin D Merck C8273-1MG Adenosine 5′-diphosphate sodium salt Sigma-Aldrich A2754-100MG PSB 0739 Tocris 3983 liposaccharide (LPS) Sigma L5024-10MG Heparin Sigma H3393-50KU Pluroni F-68 Non-ionic Surfactant (100X) Thermofisher 24040032 ProLongTM Gold Antifade Mountant ThermoFisher P36930 DMSO ThermoFisher Scientific D12345 Normal Goat Serum Abcam ab7481 Triton X-100 VWR 437002A Paraformaldehyde, 4% in PBS alfa aesar J19943 Dibenzylether Sigma-Aldrich 108014-1KG Dichloromethane Carlroth 8424.4 Methanol, 2,5L VWR 20847307 eBioscience 1X RBC Lysis Buffer ThermoFisher Scientific 00-4333-57 Percoll PLUS Sigma E0414-1L Maxpar Cell Staining Buffer Fluidigm 201068 Pierce 16% Formaldehyde (w/v), Methanol-free ThermoFisher 28908 Maxpar Cell Acquisition Solution Fluidigm 201237 Maxpar EQ Four Element Calibration Beads Fluidigm 201078 autoMACS Running Buffer – MACS Separation Buffer Miltenyi Biotech 130-091-221 Actinomycin D Sigma A1410 Triptolide Sigma T3652 Anisomycin Sigma A9789 1X HBSS Thermofisher 14175053 (Continued on next page) e3 Cell Reports Methods 6, 101362, April 20, 2026 Article ll OPEN ACCESS
Membrane:Article Title: Immune response to adeno-associated viral vectors in a human iPSC-derived microglia chimeric mouse model.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IBA1 antibody Wako 019–19741 anti-GFP antibody Aves GFP-1010 Goat anti-Rabbit IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 790 ThermoFisher Scientific A11369 Goat anti-Rabbit IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor Plus 647 ThermoFisher Scientific A32733 Goat anti-Chicken IgY (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ThermoFisher Scientific A32931 Goat Anti-Chicken IgY H&L (Alexa Fluor 647) Abcam ab150171 Ly-6G Fluidigm 3141008B Ly-6C Biolegend 128039 CD4 Fluidigm 3145002B F4/80 Fluidigm 3146008B CD11b Fluidigm 3148003B CD68 Biolegend 137002 CD3e Fluidigm 3152004B CD8a Fluidigm 3153012B TER-119 Fluidigm 3154005B CD11c Fluidigm 3162017B CD19 Fluidigm 3166015B CD335 Fluidigm 3167008B CD117 Fluidigm 3173004B CD127 Fluidigm 3175006B FceRIa Fluidigm 3176006B MHC II Fluidigm 3209006B CD25 biolegend 101913 Siglec-F biolegend 155502 CD45 Fluidigm Europe BV 3089005B RAGE Abcam ab228861 Tim-3 BioLegend 345019 TREM2 R&D Systems MAB17291 CD279 (PD-1) BioLegend 329941 M6PR Abcam ab236067 CD206 (MMR) BioLegend 141702 HLA-A,B,C Fluidigm Europe BV 3141010B TGFBR1 Abcam ab51871 GM-CSFR/CSF2R R&D Systems MAB706 CD83 Biolegend 305302 EMR1 Biorad MCA2674GA CD64 Fluidigm Europe BV 3146006B CD11c Fluidigm Europe BV 3147008B CD16 Fluidigm Europe BV 3148004B CD68 Biolegend 137002 (Continued on next page) e1 Cell Reports Methods 6, 101362, April 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER CD169/SIGLEC Biolegend 346002 CLEC7A (Dectin-1) Biolegend 355402 CD3e Fluidigm Europe BV 3152004B CD192 (CCR2) Fluidigm Europe BV 3153023B CD45 Fluidigm Europe BV 3154001B CD36 Fluidigm Europe BV 3155012B CD86 Fluidigm Europe BV 3156008B CD284 (TLR4) Fluidigm Europe BV 3158024B CD115/CSF1R R&D Systems MAB329 CD80 Fluidigm Europe BV 3161023B CD95 Fluidigm Europe BV 3162038B CD172a/b (SIRPa/b) Fluidigm Europe BV 3163017B CX3CR1 Fluidigm Europe BV 3164023B CD40 Fluidigm Europe BV 3165005B CD204/MSR1 biolegend 371902 CD11b Fluidigm Europe BV 3167011B CD206 (MMR) Fluidigm Europe BV 3168008B CD33 Fluidigm Europe BV 3169010B C3aR Biolegend 345802 CD68 Fluidigm Europe BV 3171011B CX3CR1 Fluidigm Europe BV 3172017B HLA-DR Fluidigm Europe BV 3173005B I-A/I-E (MHCII) Fluidigm Europe BV 3174003B CD14 Fluidigm Europe BV 3175015B CD282 (TLR2) Fluidigm Europe BV 3176021B CD274/PD-L1 (MIH1) Fluidigm Europe BV 3209014B Bacterial and virus strains AAV6-mAIF1-RFP Vector Biolabs Custom order AAV2-CAG-RFP Vector Biolabs Custom order AAV9-CAG-RFP Vector Biolabs Custom order Chemicals, peptides, and recombinant proteins Anti-adherence rinsing solution Stem Cell Technologies 07010 Gibco DPBS, no calcium, no magnesium ThermoFisher Scientific 14190250 Accutase solution Merck A6964 Matrigel Growth factor reduced, phenol red-free, *LDEV-free Corning 356231 Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane Merck L2020 Essential 8 Flex Medium Kit ThermoFisher Scientific A2858501 IMDM medium ThermoFisher Scientific 21980032 Ham’s F-12 Nutrient Mix ThermoFisher Scientific 11765054 Phenol-free DMEM/F12 medium ThermoFisher Scientific 11039021 Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) ThermoFischer Scientific 51500056 Insulin-Transferrin-Selenium (ITS-G) ThermoFischer Scientific 41400045 L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate Merck A8960-5G 1-Thioglycerol Merck M-1753-100ML Poly(vinyl alcohol) Merck P8136-250G GlutaMAX Supplement ThermoFisher Scientific 35050061 (Continued on next page) Cell Reports Methods 6, 101362, April 20, 2026 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemically-defined lipid concentrate ThermoFisher Scientific 11905031 MEM Non-Essential Amino Acids Solution ThermoFisher Scientific 11140035 N2 supplements ThermoFisher Scientific 17502048 B27 supplements ThermoFisher Scientific 17504044 Insulin human Merck I2643-50mG Rho kinase inhibitor (Y-27632 dihydrochloride) Abcam ab120129 Recombinant Human IL-3 Peprotech 200–03 Recombinant Human IL-6 Peprotech 200–06 C-Kit Ligand (SCF) Recombinant Human Protein ThermoFisher Scientific PHC2115 Lithium chloride solution Merck L7026-100ML FGF-Basic (AA 1–155) Recombinant Human Protein ThermoFisher Scientific PHG0266 BMP4 Recombinant Human Protein ThermoFisher Scientific PHC9534 Activin A Recombinant Human Protein ThermoFisher Scientific PHC9564 Recombinant Human VEGF 165 Peprotech 100–20 Thrombopoietin (TPO) Recombinant Human Protein ThermoFisher Scientific PHC9514 Recombinant Human M-CSF PeproTech 300–25 Human TGF-β1 PeproTech 100–21 Recombinant Human IL-34 PeproTech 200–34 DMEM-F12 ThermoFisher Scientific 11320033 mTeSRTM Plus medium Stemcell Technologies 05825 CryoStor CS10 Stemcell Technologies 07959 pHrodo Red Zymosan Bioparticles Invitrogen P35364 Cytochalasin D Merck C8273-1MG Adenosine 5′-diphosphate sodium salt Sigma-Aldrich A2754-100MG PSB 0739 Tocris 3983 liposaccharide (LPS) Sigma L5024-10MG Heparin Sigma H3393-50KU Pluroni F-68 Non-ionic Surfactant (100X) Thermofisher 24040032 ProLongTM Gold Antifade Mountant ThermoFisher P36930 DMSO ThermoFisher Scientific D12345 Normal Goat Serum Abcam ab7481 Triton X-100 VWR 437002A Paraformaldehyde, 4% in PBS alfa aesar J19943 Dibenzylether Sigma-Aldrich 108014-1KG Dichloromethane Carlroth 8424.4 Methanol, 2,5L VWR 20847307 eBioscience 1X RBC Lysis Buffer ThermoFisher Scientific 00-4333-57 Percoll PLUS Sigma E0414-1L Maxpar Cell Staining Buffer Fluidigm 201068 Pierce 16% Formaldehyde (w/v), Methanol-free ThermoFisher 28908 Maxpar Cell Acquisition Solution Fluidigm 201237 Maxpar EQ Four Element Calibration Beads Fluidigm 201078 autoMACS Running Buffer – MACS Separation Buffer Miltenyi Biotech 130-091-221 Actinomycin D Sigma A1410 Triptolide Sigma T3652 Anisomycin Sigma A9789 1X HBSS Thermofisher 14175053 (Continued on next page) e3 Cell Reports Methods 6, 101362, April 20, 2026 Article ll OPEN ACCESS
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