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cd8a t cell isolation kit  (Miltenyi Biotec)


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    Miltenyi Biotec cd8a t cell isolation kit
    Cd8a T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/CD8a%2B+T+Cell+Isolation+Kit%2C+mouse/pmc13125905-58-0-7
    Average 97 stars, based on 642 article reviews
    cd8a t cell isolation kit - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Optimizing CRISPR–Cas9-mediated TCR knockout in primary mouse CD8+, CD4+, and regulatory T cells
    Article Snippet: .. Isolation of splenocytes was followed by magnetic T cell isolation using the Mouse CD4+ T Cell Isolation kit (130-104-454; Miltenyi Biotec) or the Mouse CD8a+ T Cell Isolation kit (130-104-075; Miltenyi Biotec) according to the manufacturer’s instructions. .. Enriched CD4+ and CD8+ cells were pooled together in a 1:1 ratio and activated using the Dynabeads Mouse T-Activator CD3/CD28 activation beads (11456D; Gibco) at a 2:1 bead-to-cell ratio.

    Article Title: Optimized Epitope-Flanking Sequences Enhance Antigen Presentation and T-Cell Responses Following mRNA Vaccination.
    Article Snippet: Red blood cells in splenocyte and bone marrow suspensions were lysed with RBC lysing solution (Cat. 420331, BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions. .. RBC-depleted cell suspensions from the spleen, lymph nodes, and bone marrow were then either used directly for T-cell phenotype staining or subjected to CD8+ T-cell enrichment using the Mouse CD8+ T-cell Isolation Kit (cat. 130-104-075, Miltenyi Biotec, Gaithersburg, MD, USA) according to the manufacturer’s instructions. .. For all FACS experiments, cells were stained for viability assessment with Zombie Aqua Fixable viability dye (Cat. 77143, BioLegend) for 15 min at room temperature, and nonspecific antibody binding was prevented using Mouse BD FC Block (Cat. 553141, BD Biosciences, Mississauga, ON, Canada).

    Article Title: p38α orchestrates BRAFV600E-melanoma progression and immunotherapy response via tumor fitness and immune microenvironment modulation
    Article Snippet: .. Naïve CD8+ T cells were isolated from spleens from donor OT-I mice (C57BL/6-Tg(TcraTcrb)1100Mjb) using the CD8+ T cell isolation kit (#130-104-075, Miltenyi Biotec) following the manufacturer’s instructions. .. The isolated CD8+ T cells were either used directly or activated with anti-CD3e (2μg/ml, #16-0031-85, eBioscience) and anti-CD28 (1μg/ml, #16-0281-85, eBioscience) for 16 h in complete T cellmedium.

    Article Title: Host Metadherin Coordinates Hepatic Lipid metabolism and CD8 + T-Cell Immunity to Promote Tumor Progression
    Article Snippet: .. Splenic CD8 + T cells from normal mice were isolated using a MACS kit (Miltenyi Biotec, #130–104-075). ..

    Article Title: Immune Circuit Rewiring by HDAC Inhibition Enables Response to Dual Checkpoint Blockade in Metastatic Breast Cancer
    Article Snippet: .. CD8+ T cells were isolated from spleens using the mouse CD8a+ T Cell Isolation Kit (Miltenyi, cat. #130-104-075) following the manufacturer’s directions, stained with CellTrace − CFSE dye (Thermo Fisher Scientific, cat. # C34554 ), and stimulated with mouse T-Activator CD3/CD28 Dynabeads − (Thermo Fisher Scientific, cat. #11452D). ..

    Article Title: p38α orchestrates BRAF V600E -melanoma progression and immunotherapy response via tumor fitness and immune microenvironment modulation
    Article Snippet: .. Naïve CD8 + T cells were isolated from spleens from donor OT-I mice (C57BL/6- Tg ( TcraTcrb )1100Mjb) using the CD8 + T cell isolation kit (#130-104-075, Miltenyi Biotec) following the manufacturer’s instructions. .. The isolated CD8 + T cells were either used directly or activated with anti-CD3e (2 μg/ml, #16-0031-85, eBioscience) and anti-CD28 (1 μg/ml, #16-0281-85, eBioscience) for 16 h in complete T cell medium.

    Article Title: SARS-CoV-2 RBD fusion ameliorates enhanced disease induced by RSV G CCD mRNA vaccine in BALB/c mice
    Article Snippet: .. Briefly, the spleens collected from immunized mice were homogenized into single-cell suspensions, and CD8+ T cells were isolated using a CD8a+ T cell Isolation Kit (Miltenyi, cat130-104-075). .. 5×105 of splenic lymphocytes or CD8+ T cells were seeded into the ELISpot plates precoated with anti-mouse IFN-γ/IL-4/IL-5 antibody (Mabtech, cat#3321-4APW/3311- AR TI CL E IN P RE SS 4APW, BD, cat#551075).

    Cell Isolation:

    Article Title: Optimizing CRISPR–Cas9-mediated TCR knockout in primary mouse CD8+, CD4+, and regulatory T cells
    Article Snippet: .. Isolation of splenocytes was followed by magnetic T cell isolation using the Mouse CD4+ T Cell Isolation kit (130-104-454; Miltenyi Biotec) or the Mouse CD8a+ T Cell Isolation kit (130-104-075; Miltenyi Biotec) according to the manufacturer’s instructions. .. Enriched CD4+ and CD8+ cells were pooled together in a 1:1 ratio and activated using the Dynabeads Mouse T-Activator CD3/CD28 activation beads (11456D; Gibco) at a 2:1 bead-to-cell ratio.

    Article Title: p38α orchestrates BRAFV600E-melanoma progression and immunotherapy response via tumor fitness and immune microenvironment modulation
    Article Snippet: .. Naïve CD8+ T cells were isolated from spleens from donor OT-I mice (C57BL/6-Tg(TcraTcrb)1100Mjb) using the CD8+ T cell isolation kit (#130-104-075, Miltenyi Biotec) following the manufacturer’s instructions. .. The isolated CD8+ T cells were either used directly or activated with anti-CD3e (2μg/ml, #16-0031-85, eBioscience) and anti-CD28 (1μg/ml, #16-0281-85, eBioscience) for 16 h in complete T cellmedium.

    Article Title: Polymeric Nanoparticle Gene Delivery to Reprogram the Tumor Immune Microenvironment in Ovarian Cancer for an Anti-Tumor Response.
    Article Snippet: After 24 hours of co-culture with mouse splenocytes, the supernatant was collected and analyzed via ELISA (BioLegend, catalog number 430804). .. For co-culture with purified cytotoxic T cells, splenocytes collected from C57BL/6J mice (8 weeks, female) were sorted via magnet-activated cell sorting (MACS) columns using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-104-075) to yield untouched CD8+ T cells. ..

    Article Title: Immune Circuit Rewiring by HDAC Inhibition Enables Response to Dual Checkpoint Blockade in Metastatic Breast Cancer
    Article Snippet: .. CD8+ T cells were isolated from spleens using the mouse CD8a+ T Cell Isolation Kit (Miltenyi, cat. #130-104-075) following the manufacturer’s directions, stained with CellTrace − CFSE dye (Thermo Fisher Scientific, cat. # C34554 ), and stimulated with mouse T-Activator CD3/CD28 Dynabeads − (Thermo Fisher Scientific, cat. #11452D). ..

    Article Title: p38α orchestrates BRAF V600E -melanoma progression and immunotherapy response via tumor fitness and immune microenvironment modulation
    Article Snippet: .. Naïve CD8 + T cells were isolated from spleens from donor OT-I mice (C57BL/6- Tg ( TcraTcrb )1100Mjb) using the CD8 + T cell isolation kit (#130-104-075, Miltenyi Biotec) following the manufacturer’s instructions. .. The isolated CD8 + T cells were either used directly or activated with anti-CD3e (2 μg/ml, #16-0031-85, eBioscience) and anti-CD28 (1 μg/ml, #16-0281-85, eBioscience) for 16 h in complete T cell medium.

    Article Title: SARS-CoV-2 RBD fusion ameliorates enhanced disease induced by RSV G CCD mRNA vaccine in BALB/c mice
    Article Snippet: .. Briefly, the spleens collected from immunized mice were homogenized into single-cell suspensions, and CD8+ T cells were isolated using a CD8a+ T cell Isolation Kit (Miltenyi, cat130-104-075). .. 5×105 of splenic lymphocytes or CD8+ T cells were seeded into the ELISpot plates precoated with anti-mouse IFN-γ/IL-4/IL-5 antibody (Mabtech, cat#3321-4APW/3311- AR TI CL E IN P RE SS 4APW, BD, cat#551075).

    Staining:

    Article Title: Optimized Epitope-Flanking Sequences Enhance Antigen Presentation and T-Cell Responses Following mRNA Vaccination.
    Article Snippet: Red blood cells in splenocyte and bone marrow suspensions were lysed with RBC lysing solution (Cat. 420331, BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions. .. RBC-depleted cell suspensions from the spleen, lymph nodes, and bone marrow were then either used directly for T-cell phenotype staining or subjected to CD8+ T-cell enrichment using the Mouse CD8+ T-cell Isolation Kit (cat. 130-104-075, Miltenyi Biotec, Gaithersburg, MD, USA) according to the manufacturer’s instructions. .. For all FACS experiments, cells were stained for viability assessment with Zombie Aqua Fixable viability dye (Cat. 77143, BioLegend) for 15 min at room temperature, and nonspecific antibody binding was prevented using Mouse BD FC Block (Cat. 553141, BD Biosciences, Mississauga, ON, Canada).

    Article Title: Immune Circuit Rewiring by HDAC Inhibition Enables Response to Dual Checkpoint Blockade in Metastatic Breast Cancer
    Article Snippet: .. CD8+ T cells were isolated from spleens using the mouse CD8a+ T Cell Isolation Kit (Miltenyi, cat. #130-104-075) following the manufacturer’s directions, stained with CellTrace − CFSE dye (Thermo Fisher Scientific, cat. # C34554 ), and stimulated with mouse T-Activator CD3/CD28 Dynabeads − (Thermo Fisher Scientific, cat. #11452D). ..

    Magnetic Cell Separation:

    Article Title: Host Metadherin Coordinates Hepatic Lipid metabolism and CD8 + T-Cell Immunity to Promote Tumor Progression
    Article Snippet: .. Splenic CD8 + T cells from normal mice were isolated using a MACS kit (Miltenyi Biotec, #130–104-075). ..

    Article Title: Polymeric Nanoparticle Gene Delivery to Reprogram the Tumor Immune Microenvironment in Ovarian Cancer for an Anti-Tumor Response.
    Article Snippet: After 24 hours of co-culture with mouse splenocytes, the supernatant was collected and analyzed via ELISA (BioLegend, catalog number 430804). .. For co-culture with purified cytotoxic T cells, splenocytes collected from C57BL/6J mice (8 weeks, female) were sorted via magnet-activated cell sorting (MACS) columns using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-104-075) to yield untouched CD8+ T cells. ..

    Purification:

    Article Title: Polymeric Nanoparticle Gene Delivery to Reprogram the Tumor Immune Microenvironment in Ovarian Cancer for an Anti-Tumor Response.
    Article Snippet: After 24 hours of co-culture with mouse splenocytes, the supernatant was collected and analyzed via ELISA (BioLegend, catalog number 430804). .. For co-culture with purified cytotoxic T cells, splenocytes collected from C57BL/6J mice (8 weeks, female) were sorted via magnet-activated cell sorting (MACS) columns using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-104-075) to yield untouched CD8+ T cells. ..

    FACS:

    Article Title: Polymeric Nanoparticle Gene Delivery to Reprogram the Tumor Immune Microenvironment in Ovarian Cancer for an Anti-Tumor Response.
    Article Snippet: After 24 hours of co-culture with mouse splenocytes, the supernatant was collected and analyzed via ELISA (BioLegend, catalog number 430804). .. For co-culture with purified cytotoxic T cells, splenocytes collected from C57BL/6J mice (8 weeks, female) were sorted via magnet-activated cell sorting (MACS) columns using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-104-075) to yield untouched CD8+ T cells. ..

    Single Cell:

    Article Title: SARS-CoV-2 RBD fusion ameliorates enhanced disease induced by RSV G CCD mRNA vaccine in BALB/c mice
    Article Snippet: .. Briefly, the spleens collected from immunized mice were homogenized into single-cell suspensions, and CD8+ T cells were isolated using a CD8a+ T cell Isolation Kit (Miltenyi, cat130-104-075). .. 5×105 of splenic lymphocytes or CD8+ T cells were seeded into the ELISpot plates precoated with anti-mouse IFN-γ/IL-4/IL-5 antibody (Mabtech, cat#3321-4APW/3311- AR TI CL E IN P RE SS 4APW, BD, cat#551075).



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    Senescence-associated GZMK⁺CD8⁺ T cells exhibit significant interactions with SS acinar cells. (A) UMAP plot showing the re-annotation and subclassification of T/NK cell populations into 14 distinct subsets from labial gland samples. (B) Bar graph displaying the proportional composition of T cell subsets in young and elderly SS patients and age- and sex-matched HCs, highlighting the increased proportion of GZMK⁺CD8⁺ T cells in SS patients. (C) The key markers associated with different T cell subsets in SS and HC groups. (D) Boxplot showing the proportion of GZMK⁺CD8⁺ T cells in the young and elderly SS groups compared to young and elderly HC groups, with the highest abundance observed in the elderly SS group. (E) Volcano plot of DEGs in GZMK⁺CD8⁺ T cells from SS patients compared to HCs, showing significantly upregulated functional genes in SS. (F) Boxplots showing the expression levels of GZMK in GZMK⁺CD8⁺ T cells from young and elderly SS patients and HCs, with significantly higher expression in SS groups, especially in the elderly SS group. (G) CellChat analysis of intercellular communication between GZMK⁺CD8⁺ T cells and various resident tissue cell types in labial glands, highlighting significant interactions with acinar cells. (H) Top 15 ligand-receptor pairs involved in the interaction between GZMK⁺CD8⁺ T cells and acinar cells, demonstrating the close communication between these cell types.
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    Bio X Cell anti mouse cd8a
    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus <t>anti-CD8a</t> antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.
    Anti Mouse Cd8a, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gb21303 apc anti mouse cd8a antibody proteintech  (Proteintech)
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    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus <t>anti-CD8a</t> antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.
    Gb21303 Apc Anti Mouse Cd8a Antibody Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Senescence-associated GZMK⁺CD8⁺ T cells exhibit significant interactions with SS acinar cells. (A) UMAP plot showing the re-annotation and subclassification of T/NK cell populations into 14 distinct subsets from labial gland samples. (B) Bar graph displaying the proportional composition of T cell subsets in young and elderly SS patients and age- and sex-matched HCs, highlighting the increased proportion of GZMK⁺CD8⁺ T cells in SS patients. (C) The key markers associated with different T cell subsets in SS and HC groups. (D) Boxplot showing the proportion of GZMK⁺CD8⁺ T cells in the young and elderly SS groups compared to young and elderly HC groups, with the highest abundance observed in the elderly SS group. (E) Volcano plot of DEGs in GZMK⁺CD8⁺ T cells from SS patients compared to HCs, showing significantly upregulated functional genes in SS. (F) Boxplots showing the expression levels of GZMK in GZMK⁺CD8⁺ T cells from young and elderly SS patients and HCs, with significantly higher expression in SS groups, especially in the elderly SS group. (G) CellChat analysis of intercellular communication between GZMK⁺CD8⁺ T cells and various resident tissue cell types in labial glands, highlighting significant interactions with acinar cells. (H) Top 15 ligand-receptor pairs involved in the interaction between GZMK⁺CD8⁺ T cells and acinar cells, demonstrating the close communication between these cell types.

    Journal: International Journal of Biological Sciences

    Article Title: GZMK⁺CD8⁺ T Cells Induce Acinar Cell Senescence and Salivary Secretion Impairment in Sjögren's Syndrome via Mitochondrial Dysfunction and TMEM16A Chloride Channel Disruption

    doi: 10.7150/ijbs.134478

    Figure Lengend Snippet: Senescence-associated GZMK⁺CD8⁺ T cells exhibit significant interactions with SS acinar cells. (A) UMAP plot showing the re-annotation and subclassification of T/NK cell populations into 14 distinct subsets from labial gland samples. (B) Bar graph displaying the proportional composition of T cell subsets in young and elderly SS patients and age- and sex-matched HCs, highlighting the increased proportion of GZMK⁺CD8⁺ T cells in SS patients. (C) The key markers associated with different T cell subsets in SS and HC groups. (D) Boxplot showing the proportion of GZMK⁺CD8⁺ T cells in the young and elderly SS groups compared to young and elderly HC groups, with the highest abundance observed in the elderly SS group. (E) Volcano plot of DEGs in GZMK⁺CD8⁺ T cells from SS patients compared to HCs, showing significantly upregulated functional genes in SS. (F) Boxplots showing the expression levels of GZMK in GZMK⁺CD8⁺ T cells from young and elderly SS patients and HCs, with significantly higher expression in SS groups, especially in the elderly SS group. (G) CellChat analysis of intercellular communication between GZMK⁺CD8⁺ T cells and various resident tissue cell types in labial glands, highlighting significant interactions with acinar cells. (H) Top 15 ligand-receptor pairs involved in the interaction between GZMK⁺CD8⁺ T cells and acinar cells, demonstrating the close communication between these cell types.

    Article Snippet: CD8+ T cells were purified by positive selection using rat CD8a MicroBeads (130-090-318, Miltenyi Biotec, Germany) according to the manufacturer's instructions.

    Techniques: Functional Assay, Expressing, Analysis, Tissue

    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus anti-CD8a antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.

    Journal: Cancer Discovery

    Article Title: Senescent-Like Neutrophils Shape Angiogenic Immunosuppressive Niches in Colorectal Cancer Liver Metastasis

    doi: 10.1158/2159-8290.CD-25-0820

    Figure Lengend Snippet: Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus anti-CD8a antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.

    Article Snippet: For CD8 + T-cell depletion, 200 μg anti-mouse CD8a (Bio X Cell, BE0061, RRID: AB_1125541) was injected intraperitoneally twice a week.

    Techniques: Comparison, Expressing, Staining, Analysis, Migration, Control