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anti rat cd8α microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec anti rat cd8α microbeads
    Semiquantitative evaluation of protein expression using proteome profiler arrays. Representative protein profiler arrays of defects treated with hADM, hADM + BMC, or hADM + BMC-CD8 after three days are shown in ( A ). The differentially expressed proteins are marked by a red square. Protein expression was analyzed denitometrically and spot density was normalized to control spots located in the upper left, upper right, and lower left corners. Heatmap generated on basis of protein expression is shown in ( B ). Heatmap contains data from differentially pretreated hADM recovered on D3 and 7. Proteins newly secreted on D7 are marked in red. Color code indicates fold change. The pie charts ( C ) show the number of secreted proteins from membranes that covered the bone defect for 3 (upper pie chart) and 7 days (lower pie chart). The categories inflammation (red), anti-inflammation (blue) and regeneration (orange) are shown. Bi- or multifunctional proteins were assigned to the respective categories, so that the sum of the fractions is higher than the total number of proteins detected. There was a general increase in the number of expressed proteins from D3 to D7. Detected proteins and their main biological functions is listed in .
    Anti Rat Cd8α Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/CD8a+MicroBeads%2C+rat/pmc12897119-80-16-20
    Average 95 stars, based on 102 article reviews
    anti rat cd8α microbeads - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Cell Supported Single Membrane Technique for the Treatment of Large Bone Defects: Depletion of CD8 + Cells Enhances Bone Healing Mechanisms During the Early Bone Healing Phase"

    Article Title: Cell Supported Single Membrane Technique for the Treatment of Large Bone Defects: Depletion of CD8 + Cells Enhances Bone Healing Mechanisms During the Early Bone Healing Phase

    Journal: Cells

    doi: 10.3390/cells15030215

    Semiquantitative evaluation of protein expression using proteome profiler arrays. Representative protein profiler arrays of defects treated with hADM, hADM + BMC, or hADM + BMC-CD8 after three days are shown in ( A ). The differentially expressed proteins are marked by a red square. Protein expression was analyzed denitometrically and spot density was normalized to control spots located in the upper left, upper right, and lower left corners. Heatmap generated on basis of protein expression is shown in ( B ). Heatmap contains data from differentially pretreated hADM recovered on D3 and 7. Proteins newly secreted on D7 are marked in red. Color code indicates fold change. The pie charts ( C ) show the number of secreted proteins from membranes that covered the bone defect for 3 (upper pie chart) and 7 days (lower pie chart). The categories inflammation (red), anti-inflammation (blue) and regeneration (orange) are shown. Bi- or multifunctional proteins were assigned to the respective categories, so that the sum of the fractions is higher than the total number of proteins detected. There was a general increase in the number of expressed proteins from D3 to D7. Detected proteins and their main biological functions is listed in .
    Figure Legend Snippet: Semiquantitative evaluation of protein expression using proteome profiler arrays. Representative protein profiler arrays of defects treated with hADM, hADM + BMC, or hADM + BMC-CD8 after three days are shown in ( A ). The differentially expressed proteins are marked by a red square. Protein expression was analyzed denitometrically and spot density was normalized to control spots located in the upper left, upper right, and lower left corners. Heatmap generated on basis of protein expression is shown in ( B ). Heatmap contains data from differentially pretreated hADM recovered on D3 and 7. Proteins newly secreted on D7 are marked in red. Color code indicates fold change. The pie charts ( C ) show the number of secreted proteins from membranes that covered the bone defect for 3 (upper pie chart) and 7 days (lower pie chart). The categories inflammation (red), anti-inflammation (blue) and regeneration (orange) are shown. Bi- or multifunctional proteins were assigned to the respective categories, so that the sum of the fractions is higher than the total number of proteins detected. There was a general increase in the number of expressed proteins from D3 to D7. Detected proteins and their main biological functions is listed in .

    Techniques Used: Expressing, Control, Generated

    Protein profiler analysis of conditioned media from defects treated with hADM, hADM + BMC, or hADM + BMC-CD8 after three days. Protein levels are normalized to the intensity of the control spots (=100%). Only proteins with significant differences ( p < 0.05) or trends ( p < 0.1) are shown. Data are presented as boxplots (median, minimum, maximum; n = 3 per group). * indicates explorative significance ( p < 0.05) versus the respective comparison group.
    Figure Legend Snippet: Protein profiler analysis of conditioned media from defects treated with hADM, hADM + BMC, or hADM + BMC-CD8 after three days. Protein levels are normalized to the intensity of the control spots (=100%). Only proteins with significant differences ( p < 0.05) or trends ( p < 0.1) are shown. Data are presented as boxplots (median, minimum, maximum; n = 3 per group). * indicates explorative significance ( p < 0.05) versus the respective comparison group.

    Techniques Used: Control, Comparison

    Protein profiler analysis of conditioned media from defects treated with hADM, hADM + BMC, or hADM + BMC-CD8 after 7 days . Protein levels are normalized to the intensity of the control spots (=100%). Only proteins with significant differences ( p < 0.05) or trends ( p < 0.1) are shown and presented in alphabetical order. Data are presented as boxplots (median, minimum, maximum; n = 3 per group). * indicates explorative significance ( p < 0.05) versus the respective comparison groups.
    Figure Legend Snippet: Protein profiler analysis of conditioned media from defects treated with hADM, hADM + BMC, or hADM + BMC-CD8 after 7 days . Protein levels are normalized to the intensity of the control spots (=100%). Only proteins with significant differences ( p < 0.05) or trends ( p < 0.1) are shown and presented in alphabetical order. Data are presented as boxplots (median, minimum, maximum; n = 3 per group). * indicates explorative significance ( p < 0.05) versus the respective comparison groups.

    Techniques Used: Control, Comparison

    Direct comparison of protein profiler data obtained after 3 and 7 days in defects treated with hADM (white), hADM + BMC (light gray), or hADM + BMC-CD8 (dark gray). Values are normalized to the density of the control spots (=100%). Only proteins with significant differences ( p < 0.05) or trends ( p < 0.1) are shown and presented in alphabetical order. Data are presented as boxplots (median, minimum, maximum; n = 3 per group). * indicates explorative significance ( p < 0.05) versus the respective comparison group.
    Figure Legend Snippet: Direct comparison of protein profiler data obtained after 3 and 7 days in defects treated with hADM (white), hADM + BMC (light gray), or hADM + BMC-CD8 (dark gray). Values are normalized to the density of the control spots (=100%). Only proteins with significant differences ( p < 0.05) or trends ( p < 0.1) are shown and presented in alphabetical order. Data are presented as boxplots (median, minimum, maximum; n = 3 per group). * indicates explorative significance ( p < 0.05) versus the respective comparison group.

    Techniques Used: Comparison, Control

    Osteogenic gene expression (A–D) and ALP protein expression (E) in MSC after cultivation in osteogenic differentiation medium (ODM) supplemented with CM. CM was generated from hADM (white), hADM seeded with BMC (light gray), or hADM seeded with CD8-depleted BMC (BMC-CD8, dark gray) wrapped around the bone defect for 3 or 7 days, then explanted and incubated in medium for 24 h. MSC were cultured for 3 weeks in ODM, with 10% CM added during the first 3 days, followed by analysis of gene expression relative to the Gapdh housekeeping gene ( A – D ) or ALP measurement ( E ). Control MSC (salmon) were cultured in expansion medium (M, only in E ) or in ODM, both without CM. # = p < 0.05 vs. ODM; * = p < 0.05 vs. indicated group. All significances are exploratory.
    Figure Legend Snippet: Osteogenic gene expression (A–D) and ALP protein expression (E) in MSC after cultivation in osteogenic differentiation medium (ODM) supplemented with CM. CM was generated from hADM (white), hADM seeded with BMC (light gray), or hADM seeded with CD8-depleted BMC (BMC-CD8, dark gray) wrapped around the bone defect for 3 or 7 days, then explanted and incubated in medium for 24 h. MSC were cultured for 3 weeks in ODM, with 10% CM added during the first 3 days, followed by analysis of gene expression relative to the Gapdh housekeeping gene ( A – D ) or ALP measurement ( E ). Control MSC (salmon) were cultured in expansion medium (M, only in E ) or in ODM, both without CM. # = p < 0.05 vs. ODM; * = p < 0.05 vs. indicated group. All significances are exploratory.

    Techniques Used: Gene Expression, Expressing, Generated, Incubation, Cell Culture, Control

    Influence of CM on expression of M1 ( Cd80, Nos2 ) and M2 ( Arg1, Socs1 ) marker genes in macrophages differentiated into M0 (A–D), M1 (E,F), or M2 (G,H). CM was generated from hADM (white), hADM seeded with BMC (light gray), or hADM seeded with CD8-depleted BMC (BMC-CD8, dark gray) wrapped around the bone defect for 3 or 7 days, then explanted and incubated in medium for 24 h. Primary rat monocytes from bone marrow aspirates were cultured for 7 days, with 10% CM added during the first 3 days. Monocytes cultured in differentiation media without CM served as controls (salmon). # = p < 0.05 vs. control; * = p < 0.05 vs. indicated group. All significances are exploratory.
    Figure Legend Snippet: Influence of CM on expression of M1 ( Cd80, Nos2 ) and M2 ( Arg1, Socs1 ) marker genes in macrophages differentiated into M0 (A–D), M1 (E,F), or M2 (G,H). CM was generated from hADM (white), hADM seeded with BMC (light gray), or hADM seeded with CD8-depleted BMC (BMC-CD8, dark gray) wrapped around the bone defect for 3 or 7 days, then explanted and incubated in medium for 24 h. Primary rat monocytes from bone marrow aspirates were cultured for 7 days, with 10% CM added during the first 3 days. Monocytes cultured in differentiation media without CM served as controls (salmon). # = p < 0.05 vs. control; * = p < 0.05 vs. indicated group. All significances are exploratory.

    Techniques Used: Expressing, Marker, Generated, Incubation, Cell Culture, Control

    Influence of CM on expression of CCL2 and IL-1RA genes in macrophages differentiated into M0 (A,B), M1 (C,D), or M2 (E,F). CM was generated from hADM (white), hADM seeded with BMC (light gray), or hADM seeded with CD8-depleted BMC (BMC-CD8, dark gray) wrapped around the bone defect for 3 or 7 days, then explanted and incubated in medium for 24 h. Primary rat monocytes from bone marrow aspirates were cultured for 7 days, with 10% CM added during the first 3 days. Monocytes cultured in differentiation media without CM served as controls (salmon). # = p < 0.05 vs. control; * = p < 0.05 vs. indicated group. All significances are exploratory.
    Figure Legend Snippet: Influence of CM on expression of CCL2 and IL-1RA genes in macrophages differentiated into M0 (A,B), M1 (C,D), or M2 (E,F). CM was generated from hADM (white), hADM seeded with BMC (light gray), or hADM seeded with CD8-depleted BMC (BMC-CD8, dark gray) wrapped around the bone defect for 3 or 7 days, then explanted and incubated in medium for 24 h. Primary rat monocytes from bone marrow aspirates were cultured for 7 days, with 10% CM added during the first 3 days. Monocytes cultured in differentiation media without CM served as controls (salmon). # = p < 0.05 vs. control; * = p < 0.05 vs. indicated group. All significances are exploratory.

    Techniques Used: Expressing, Generated, Incubation, Cell Culture, Control

    Related Articles

    Selection:

    Article Title: Recent thymic emigrants preferentially undergo memory inflation after persistent infection.
    Article Snippet: .. CD8 + T cells were bead enriched from the spleens by positive selection with CD8a microbeads (Miltenyi). ..

    Article Title: CCL24 recruits CCR3 + TAMs to promote immunosuppression via YAP1 activation and serves as a therapeutic target for Gracillin in colorectal cancer
    Article Snippet: .. Thymus from C57BL/6J mice (6 weeks old, Anburui Biotechnologies, Fujian, China) was mechanically dissociated to prepare a single-cell suspension, and CD8 + T cells were purified by positive selection using CD8a microbeads (Miltenyi Biotec). .. The sorted CD8 + T cells were activated with CD3/CD28 agonist antibodies (2 μg/mL, BioLegend) for 48 h. Cell function was assessed by intracellular cytokine staining and flow cytometry to evaluate TNFA and GZMB expression.

    Article Title: Cell Supported Single Membrane Technique for the Treatment of Large Bone Defects: Depletion of CD8 + Cells Enhances Bone Healing Mechanisms During the Early Bone Healing Phase
    Article Snippet: .. CD8 + cells from the BMC fraction (10 cells) were depleted by positive magnetic selection applying anti-rat CD8α MicroBeads (130-090-318, Miltenyi Biotec, Bergisch Gladbach, Germany) and LD columns (Miltenyi Biotec) thereby following the instructions of the manufacturer and described in [ ]. ..

    Article Title: Cell Supported Single Membrane Technique for the Treatment of Large Bone Defects: Depletion of CD8 + Cells Enhances Bone Healing Mechanisms During the Early Bone Healing Phase.
    Article Snippet: .. CD8+ cells from the BMC fraction (107 cells) were depleted by positive magnetic selection applying anti-rat CD8α MicroBeads (130-090-318, Miltenyi Biotec, Bergisch Gladbach, Germany) and LD columns (Miltenyi Biotec) thereby following the instructions of the https://doi.org/10.3390/cells15030215 manufacturer and described in [7]. ..

    Single Cell:

    Article Title: CCL24 recruits CCR3 + TAMs to promote immunosuppression via YAP1 activation and serves as a therapeutic target for Gracillin in colorectal cancer
    Article Snippet: .. Thymus from C57BL/6J mice (6 weeks old, Anburui Biotechnologies, Fujian, China) was mechanically dissociated to prepare a single-cell suspension, and CD8 + T cells were purified by positive selection using CD8a microbeads (Miltenyi Biotec). .. The sorted CD8 + T cells were activated with CD3/CD28 agonist antibodies (2 μg/mL, BioLegend) for 48 h. Cell function was assessed by intracellular cytokine staining and flow cytometry to evaluate TNFA and GZMB expression.

    Article Title: LDLR + Monocytic Myeloid-derived Suppressor Cells Attenuate Allograft Rejection in Liver Transplantation
    Article Snippet: M-MDSCs were then isolated using magnetic microbeads (Miltenyi Biotec). .. Single-cell suspensions prepared from lymph nodes were used to isolate CD8 + T cells with CD8a microbeads (Miltenyi Biotec). ..

    Suspension:

    Article Title: CCL24 recruits CCR3 + TAMs to promote immunosuppression via YAP1 activation and serves as a therapeutic target for Gracillin in colorectal cancer
    Article Snippet: .. Thymus from C57BL/6J mice (6 weeks old, Anburui Biotechnologies, Fujian, China) was mechanically dissociated to prepare a single-cell suspension, and CD8 + T cells were purified by positive selection using CD8a microbeads (Miltenyi Biotec). .. The sorted CD8 + T cells were activated with CD3/CD28 agonist antibodies (2 μg/mL, BioLegend) for 48 h. Cell function was assessed by intracellular cytokine staining and flow cytometry to evaluate TNFA and GZMB expression.

    Purification:

    Article Title: CCL24 recruits CCR3 + TAMs to promote immunosuppression via YAP1 activation and serves as a therapeutic target for Gracillin in colorectal cancer
    Article Snippet: .. Thymus from C57BL/6J mice (6 weeks old, Anburui Biotechnologies, Fujian, China) was mechanically dissociated to prepare a single-cell suspension, and CD8 + T cells were purified by positive selection using CD8a microbeads (Miltenyi Biotec). .. The sorted CD8 + T cells were activated with CD3/CD28 agonist antibodies (2 μg/mL, BioLegend) for 48 h. Cell function was assessed by intracellular cytokine staining and flow cytometry to evaluate TNFA and GZMB expression.

    FACS:

    Article Title: Histone lactylation-driven feedback loop modulates cholesterol-linked immunosuppression in pancreatic cancer
    Article Snippet: .. Using CD8a microbeads and magnetic cell sorting (Miltenyi Biotec, Germany), CD8+ T cells were extracted from the spleens of OT-1 mice in preparation for OT-1 CD8+ T Cell adoptive transfer. ..

    Article Title: Histone lactylation-driven feedback loop modulates cholesterol-linked immunosuppression in pancreatic cancer
    Article Snippet: .. T cell isolation and T cell suppression assay Using CD8a microbeads and magnetic cell sorting (Miltenyi Biotec, Germany) in accordance with the manufacturer's procedure, CD8+ T lymphocytes were extracted from the spleens of mice. .. T cell isolation and T cell suppression assay Using CD8a microbeads and magnetic cell sorting (Miltenyi Biotec, Germany) in accordance with the manufacturer's procedure, CD8+ T lymphocytes were extracted from the spleens of mice.

    Adoptive Transfer Assay:

    Article Title: Histone lactylation-driven feedback loop modulates cholesterol-linked immunosuppression in pancreatic cancer
    Article Snippet: .. Using CD8a microbeads and magnetic cell sorting (Miltenyi Biotec, Germany), CD8+ T cells were extracted from the spleens of OT-1 mice in preparation for OT-1 CD8+ T Cell adoptive transfer. ..

    Cell Isolation:

    Article Title: Histone lactylation-driven feedback loop modulates cholesterol-linked immunosuppression in pancreatic cancer
    Article Snippet: .. T cell isolation and T cell suppression assay Using CD8a microbeads and magnetic cell sorting (Miltenyi Biotec, Germany) in accordance with the manufacturer's procedure, CD8+ T lymphocytes were extracted from the spleens of mice. .. T cell isolation and T cell suppression assay Using CD8a microbeads and magnetic cell sorting (Miltenyi Biotec, Germany) in accordance with the manufacturer's procedure, CD8+ T lymphocytes were extracted from the spleens of mice.

    Article Title: Inhibition of Galectin-3 Expression Could Preserve the Immunoregulatory Function of Mesenchymal Stem Cells During Cell Passaging
    Article Snippet: .. CD4+/CD8+ T cell isolation or depletion was performed using CD4 and CD8a MicroBeads and the AutoMACS system (Miltenyi Biotec, Germany) according to the manufacturer’s protocol. .. Briefly, 10 μL of CD4 MicroBeads and 10 μL of CD8a MicroBeads were added per 1 × 107 cells, and the mixture was incubated for 15 minutes at 4 °C in the dark.

    Suppression Assay:

    Article Title: Histone lactylation-driven feedback loop modulates cholesterol-linked immunosuppression in pancreatic cancer
    Article Snippet: .. T cell isolation and T cell suppression assay Using CD8a microbeads and magnetic cell sorting (Miltenyi Biotec, Germany) in accordance with the manufacturer's procedure, CD8+ T lymphocytes were extracted from the spleens of mice. .. T cell isolation and T cell suppression assay Using CD8a microbeads and magnetic cell sorting (Miltenyi Biotec, Germany) in accordance with the manufacturer's procedure, CD8+ T lymphocytes were extracted from the spleens of mice.



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    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus <t>anti-CD8a</t> antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.
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    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus <t>anti-CD8a</t> antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.
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    Image Search Results


    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus anti-CD8a antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.

    Journal: Cancer Discovery

    Article Title: Senescent-Like Neutrophils Shape Angiogenic Immunosuppressive Niches in Colorectal Cancer Liver Metastasis

    doi: 10.1158/2159-8290.CD-25-0820

    Figure Lengend Snippet: Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus anti-CD8a antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.

    Article Snippet: For CD8 + T-cell depletion, 200 μg anti-mouse CD8a (Bio X Cell, BE0061, RRID: AB_1125541) was injected intraperitoneally twice a week.

    Techniques: Comparison, Expressing, Staining, Analysis, Migration, Control