Review



mouse cd8 t cell isolation kit  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Miltenyi Biotec mouse cd8 t cell isolation kit
    Mouse Cd8 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/CD8a%2B+T+Cell+Isolation+Kit%2C+mouse/pm42442600-261-27-34
    Average 97 stars, based on 640 article reviews
    mouse cd8 t cell isolation kit - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Optimizing CRISPR–Cas9-mediated TCR knockout in primary mouse CD8+, CD4+, and regulatory T cells
    Article Snippet: .. Isolation of splenocytes was followed by magnetic T cell isolation using the Mouse CD4+ T Cell Isolation kit (130-104-454; Miltenyi Biotec) or the Mouse CD8a+ T Cell Isolation kit (130-104-075; Miltenyi Biotec) according to the manufacturer’s instructions. .. Enriched CD4+ and CD8+ cells were pooled together in a 1:1 ratio and activated using the Dynabeads Mouse T-Activator CD3/CD28 activation beads (11456D; Gibco) at a 2:1 bead-to-cell ratio.

    Article Title: Optimized Epitope-Flanking Sequences Enhance Antigen Presentation and T-Cell Responses Following mRNA Vaccination.
    Article Snippet: Red blood cells in splenocyte and bone marrow suspensions were lysed with RBC lysing solution (Cat. 420331, BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions. .. RBC-depleted cell suspensions from the spleen, lymph nodes, and bone marrow were then either used directly for T-cell phenotype staining or subjected to CD8+ T-cell enrichment using the Mouse CD8+ T-cell Isolation Kit (cat. 130-104-075, Miltenyi Biotec, Gaithersburg, MD, USA) according to the manufacturer’s instructions. .. For all FACS experiments, cells were stained for viability assessment with Zombie Aqua Fixable viability dye (Cat. 77143, BioLegend) for 15 min at room temperature, and nonspecific antibody binding was prevented using Mouse BD FC Block (Cat. 553141, BD Biosciences, Mississauga, ON, Canada).

    Article Title: New Therapeutic Cancer Vaccine with Lenalidomide Induces Potent T Cell Immunity and Inhibits HPV E6/E7-Expressing Tumor Growth in Mice
    Article Snippet: .. Two weeks after the last 267 vaccination, untouched splenic CD8+ T splenocytes were isolated through negative selection using 268 a mouse CD8+ T Cell Isolation Kit (Miltenyi Biotec, catalog # 130-104-075)) as described in the 269 manufacturer’s protocol. .. Two days before T cell transfer, recipient tumor-bearing mice were 270 treated with a single intraperitoneal dose of cyclophosphamide (3 mg per mouse) (Selleck 271 Chemicals, CAS: 50-18-0).

    Article Title: p38α orchestrates BRAFV600E-melanoma progression and immunotherapy response via tumor fitness and immune microenvironment modulation
    Article Snippet: .. Naïve CD8+ T cells were isolated from spleens from donor OT-I mice (C57BL/6-Tg(TcraTcrb)1100Mjb) using the CD8+ T cell isolation kit (#130-104-075, Miltenyi Biotec) following the manufacturer’s instructions. .. The isolated CD8+ T cells were either used directly or activated with anti-CD3e (2μg/ml, #16-0031-85, eBioscience) and anti-CD28 (1μg/ml, #16-0281-85, eBioscience) for 16 h in complete T cellmedium.

    Article Title: LPL-Positive Endothelial Cells Control T-cell Homing in Liver Metastasis
    Article Snippet: .. CD8+ T cells were later isolated and purified via untouched magnetically labeled antibodies (Miltenyi Biotec cat. #130-104-075). ..

    Article Title: Host Metadherin Coordinates Hepatic Lipid metabolism and CD8 + T-Cell Immunity to Promote Tumor Progression
    Article Snippet: .. Splenic CD8 + T cells from normal mice were isolated using a MACS kit (Miltenyi Biotec, #130–104-075). ..

    Article Title: Immune Circuit Rewiring by HDAC Inhibition Enables Response to Dual Checkpoint Blockade in Metastatic Breast Cancer
    Article Snippet: .. CD8+ T cells were isolated from spleens using the mouse CD8a+ T Cell Isolation Kit (Miltenyi, cat. #130-104-075) following the manufacturer’s directions, stained with CellTrace − CFSE dye (Thermo Fisher Scientific, cat. # C34554 ), and stimulated with mouse T-Activator CD3/CD28 Dynabeads − (Thermo Fisher Scientific, cat. #11452D). ..

    Cell Isolation:

    Article Title: Optimizing CRISPR–Cas9-mediated TCR knockout in primary mouse CD8+, CD4+, and regulatory T cells
    Article Snippet: .. Isolation of splenocytes was followed by magnetic T cell isolation using the Mouse CD4+ T Cell Isolation kit (130-104-454; Miltenyi Biotec) or the Mouse CD8a+ T Cell Isolation kit (130-104-075; Miltenyi Biotec) according to the manufacturer’s instructions. .. Enriched CD4+ and CD8+ cells were pooled together in a 1:1 ratio and activated using the Dynabeads Mouse T-Activator CD3/CD28 activation beads (11456D; Gibco) at a 2:1 bead-to-cell ratio.

    Article Title: New Therapeutic Cancer Vaccine with Lenalidomide Induces Potent T Cell Immunity and Inhibits HPV E6/E7-Expressing Tumor Growth in Mice
    Article Snippet: .. Two weeks after the last 267 vaccination, untouched splenic CD8+ T splenocytes were isolated through negative selection using 268 a mouse CD8+ T Cell Isolation Kit (Miltenyi Biotec, catalog # 130-104-075)) as described in the 269 manufacturer’s protocol. .. Two days before T cell transfer, recipient tumor-bearing mice were 270 treated with a single intraperitoneal dose of cyclophosphamide (3 mg per mouse) (Selleck 271 Chemicals, CAS: 50-18-0).

    Article Title: p38α orchestrates BRAFV600E-melanoma progression and immunotherapy response via tumor fitness and immune microenvironment modulation
    Article Snippet: .. Naïve CD8+ T cells were isolated from spleens from donor OT-I mice (C57BL/6-Tg(TcraTcrb)1100Mjb) using the CD8+ T cell isolation kit (#130-104-075, Miltenyi Biotec) following the manufacturer’s instructions. .. The isolated CD8+ T cells were either used directly or activated with anti-CD3e (2μg/ml, #16-0031-85, eBioscience) and anti-CD28 (1μg/ml, #16-0281-85, eBioscience) for 16 h in complete T cellmedium.

    Article Title: Polymeric Nanoparticle Gene Delivery to Reprogram the Tumor Immune Microenvironment in Ovarian Cancer for an Anti-Tumor Response.
    Article Snippet: After 24 hours of co-culture with mouse splenocytes, the supernatant was collected and analyzed via ELISA (BioLegend, catalog number 430804). .. For co-culture with purified cytotoxic T cells, splenocytes collected from C57BL/6J mice (8 weeks, female) were sorted via magnet-activated cell sorting (MACS) columns using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-104-075) to yield untouched CD8+ T cells. ..

    Article Title: Immune Circuit Rewiring by HDAC Inhibition Enables Response to Dual Checkpoint Blockade in Metastatic Breast Cancer
    Article Snippet: .. CD8+ T cells were isolated from spleens using the mouse CD8a+ T Cell Isolation Kit (Miltenyi, cat. #130-104-075) following the manufacturer’s directions, stained with CellTrace − CFSE dye (Thermo Fisher Scientific, cat. # C34554 ), and stimulated with mouse T-Activator CD3/CD28 Dynabeads − (Thermo Fisher Scientific, cat. #11452D). ..

    Staining:

    Article Title: Optimized Epitope-Flanking Sequences Enhance Antigen Presentation and T-Cell Responses Following mRNA Vaccination.
    Article Snippet: Red blood cells in splenocyte and bone marrow suspensions were lysed with RBC lysing solution (Cat. 420331, BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions. .. RBC-depleted cell suspensions from the spleen, lymph nodes, and bone marrow were then either used directly for T-cell phenotype staining or subjected to CD8+ T-cell enrichment using the Mouse CD8+ T-cell Isolation Kit (cat. 130-104-075, Miltenyi Biotec, Gaithersburg, MD, USA) according to the manufacturer’s instructions. .. For all FACS experiments, cells were stained for viability assessment with Zombie Aqua Fixable viability dye (Cat. 77143, BioLegend) for 15 min at room temperature, and nonspecific antibody binding was prevented using Mouse BD FC Block (Cat. 553141, BD Biosciences, Mississauga, ON, Canada).

    Article Title: Immune Circuit Rewiring by HDAC Inhibition Enables Response to Dual Checkpoint Blockade in Metastatic Breast Cancer
    Article Snippet: .. CD8+ T cells were isolated from spleens using the mouse CD8a+ T Cell Isolation Kit (Miltenyi, cat. #130-104-075) following the manufacturer’s directions, stained with CellTrace − CFSE dye (Thermo Fisher Scientific, cat. # C34554 ), and stimulated with mouse T-Activator CD3/CD28 Dynabeads − (Thermo Fisher Scientific, cat. #11452D). ..

    Selection:

    Article Title: New Therapeutic Cancer Vaccine with Lenalidomide Induces Potent T Cell Immunity and Inhibits HPV E6/E7-Expressing Tumor Growth in Mice
    Article Snippet: .. Two weeks after the last 267 vaccination, untouched splenic CD8+ T splenocytes were isolated through negative selection using 268 a mouse CD8+ T Cell Isolation Kit (Miltenyi Biotec, catalog # 130-104-075)) as described in the 269 manufacturer’s protocol. .. Two days before T cell transfer, recipient tumor-bearing mice were 270 treated with a single intraperitoneal dose of cyclophosphamide (3 mg per mouse) (Selleck 271 Chemicals, CAS: 50-18-0).

    Purification:

    Article Title: LPL-Positive Endothelial Cells Control T-cell Homing in Liver Metastasis
    Article Snippet: .. CD8+ T cells were later isolated and purified via untouched magnetically labeled antibodies (Miltenyi Biotec cat. #130-104-075). ..

    Article Title: Polymeric Nanoparticle Gene Delivery to Reprogram the Tumor Immune Microenvironment in Ovarian Cancer for an Anti-Tumor Response.
    Article Snippet: After 24 hours of co-culture with mouse splenocytes, the supernatant was collected and analyzed via ELISA (BioLegend, catalog number 430804). .. For co-culture with purified cytotoxic T cells, splenocytes collected from C57BL/6J mice (8 weeks, female) were sorted via magnet-activated cell sorting (MACS) columns using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-104-075) to yield untouched CD8+ T cells. ..

    Labeling:

    Article Title: LPL-Positive Endothelial Cells Control T-cell Homing in Liver Metastasis
    Article Snippet: .. CD8+ T cells were later isolated and purified via untouched magnetically labeled antibodies (Miltenyi Biotec cat. #130-104-075). ..

    FACS:

    Article Title: Polymeric Nanoparticle Gene Delivery to Reprogram the Tumor Immune Microenvironment in Ovarian Cancer for an Anti-Tumor Response.
    Article Snippet: After 24 hours of co-culture with mouse splenocytes, the supernatant was collected and analyzed via ELISA (BioLegend, catalog number 430804). .. For co-culture with purified cytotoxic T cells, splenocytes collected from C57BL/6J mice (8 weeks, female) were sorted via magnet-activated cell sorting (MACS) columns using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-104-075) to yield untouched CD8+ T cells. ..

    Magnetic Cell Separation:

    Article Title: Polymeric Nanoparticle Gene Delivery to Reprogram the Tumor Immune Microenvironment in Ovarian Cancer for an Anti-Tumor Response.
    Article Snippet: After 24 hours of co-culture with mouse splenocytes, the supernatant was collected and analyzed via ELISA (BioLegend, catalog number 430804). .. For co-culture with purified cytotoxic T cells, splenocytes collected from C57BL/6J mice (8 weeks, female) were sorted via magnet-activated cell sorting (MACS) columns using a CD8a+ T Cell Isolation Kit (Miltenyi Biotec, catalog no. 130-104-075) to yield untouched CD8+ T cells. ..

    Article Title: Host Metadherin Coordinates Hepatic Lipid metabolism and CD8 + T-Cell Immunity to Promote Tumor Progression
    Article Snippet: .. Splenic CD8 + T cells from normal mice were isolated using a MACS kit (Miltenyi Biotec, #130–104-075). ..



    Similar Products

    97
    Miltenyi Biotec mouse cd8 t cell isolation kit
    Mouse Cd8 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/CD8a%2B+T+Cell+Isolation+Kit%2C+mouse/pm42882451-142-28-35
    Average 97 stars, based on 1 article reviews
    mouse cd8 t cell isolation kit - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec magnet activated cell sorting macs columns
    Magnet Activated Cell Sorting Macs Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/CD8a%2B+T+Cell+Isolation+Kit%2C+mouse/pm42887583-76-18-30
    Average 97 stars, based on 1 article reviews
    magnet activated cell sorting macs columns - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    93
    Cytek Biosciences anti cd8a
    Anti Cd8a, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/PE-Cyanine7+Anti-Human+CD8a/pm42880242-230-60-63
    Average 93 stars, based on 1 article reviews
    anti cd8a - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    97
    Bio X Cell anti cd8a
    Anti Cd8a, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/InVivoMAb+anti-mouse+CD8%CE%B1/pm42875546-459-7-8
    Average 97 stars, based on 1 article reviews
    anti cd8a - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    94
    Miltenyi Biotec cd8αpe
    Cd8αpe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/CD8a+Antibody%2C+anti-mouse%2C+REAfinity/10__1038_slash_s41398___026___04497___2-166-30-31
    Average 94 stars, based on 1 article reviews
    cd8αpe - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    97
    Bio X Cell anti mouse cd8a
    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus <t>anti-CD8a</t> antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.
    Anti Mouse Cd8a, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/InVivoMAb+anti-mouse+CD8%CE%B1/pmc13628106-246-7-9
    Average 97 stars, based on 1 article reviews
    anti mouse cd8a - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    93
    Proteintech gb21303 apc anti mouse cd8a antibody proteintech
    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus <t>anti-CD8a</t> antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.
    Gb21303 Apc Anti Mouse Cd8a Antibody Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/APC+Anti-mouse+CD8a/pm42810343-283-261-266
    Average 93 stars, based on 1 article reviews
    gb21303 apc anti mouse cd8a antibody proteintech - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    91
    Cytek Biosciences cd8a
    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus <t>anti-CD8a</t> antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.
    Cd8a, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/APC+Anti-Human+CD8a/10__1021_slash_acsnano__6c11865-448-42-46
    Average 91 stars, based on 1 article reviews
    cd8a - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    93
    Proteintech pe anti mouse cd8
    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus <t>anti-CD8a</t> antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.
    Pe Anti Mouse Cd8, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd8a/PE+Anti-mouse+CD8a/pm42785880-55-14-21
    Average 93 stars, based on 1 article reviews
    pe anti mouse cd8 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus anti-CD8a antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.

    Journal: Cancer Discovery

    Article Title: Senescent-Like Neutrophils Shape Angiogenic Immunosuppressive Niches in Colorectal Cancer Liver Metastasis

    doi: 10.1158/2159-8290.CD-25-0820

    Figure Lengend Snippet: Proangiogenic and immunosuppression phenotype of TANs in the CRLM microenvironment. A, Heatmap showing signature scores for angiogenesis, myeloid cell recruitment, neutrophil cytotoxicity, MDSC, and NET across each TAN and PMN subset. B, Comparison of angiogenesis, myeloid cell recruitment, MDSC, and NET signature scores among TAN and PMN subsets. C, Dot plot illustrating expression levels of major cytokines and chemokines in each TAN and PMN subset. D, Representative IHC images of CD31 staining in CRLM tissues. Scale bars, 50 µm. E, Correlation analysis between CD31 and TAN1 density using Spearman’s rank correlation ( n = 102). F, Representative images and quantification of tube formation in HUVECs when cocultured with conditioned medium from human LGALS3 − neutrophils or LGALS3 + neutrophils ( n = 6). G, UMAP plot showing six monocyte/macrophage subsets across colorectal cancer, adjacent liver tissues, CRLM, and PB samples. H, Shell-like plot depicting ligand–receptor interaction strength between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. I, Selected ligand–receptor interactions between TAN1_LGASL3 and neutrophil/monocyte/macrophage subset in CRLM. J, Representative mIHC images of CD68, CCL3L1, CD66b, CCR1, and BHLHE40 in CRLM. Scale bars, 50 µm. K, Representative images of macrophage migration assays and effects of conditioned medium from human LGALS3 + neutrophils on migration of macrophages (induced from THP-1 cells) in the presence of CCR1 inhibitor (BX471) or anti-CCL3 antibody ( n = 6). L, Comparison of immunosuppressive macrophage signature scores between colorectal cancer and CRLM. M, Schematic of the CRLM mouse model. C57BL/6 mice received intrasplenic injections of mouse colorectal cancer cells (MC38). Mice were subsequently treated with IgG control (IgG; n = 6), anti-Ly6G antibody (anti-Ly6G; n = 6), or anti-Ly6G plus anti-CD8a antibodies (anti-Ly6G + anti-CD8a; n = 6). Livers were harvested on day 14. N, Representative images of liver metastases from IgG, anti-Ly6G, and anti-Ly6G + anti-CD8a groups. O, Comparison of liver weights among the three groups. P values were calculated by the Wilcoxon rank-sum test ( B and L ) and unpaired Student t test ( F , K , and O ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. CRC, colorectal cancer; Ctrl, control.

    Article Snippet: For CD8 + T-cell depletion, 200 μg anti-mouse CD8a (Bio X Cell, BE0061, RRID: AB_1125541) was injected intraperitoneally twice a week.

    Techniques: Comparison, Expressing, Staining, Analysis, Migration, Control