Review




Structured Review

Sanying Ltd cd86
In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker <t>CD86</t> and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.
Cd86, supplied by Sanying Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd86/anti+cd86/pmc12808909-58-3-9
Average 86 stars, based on 1 article reviews
cd86 - by Bioz Stars, 2026-10
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Images

1) Product Images from "Shikonin-loaded porous graphdiyne nanofilm on titanium surface for enhanced antibacterial activity and osseointegration"

Article Title: Shikonin-loaded porous graphdiyne nanofilm on titanium surface for enhanced antibacterial activity and osseointegration

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2025.12.055

In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker CD86 and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.
Figure Legend Snippet: In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker CD86 and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.

Techniques Used: In Vitro, Cell Culture, Staining, Marker, Fluorescence, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR

In vivo evaluation of osteogenesis and osseointegration. (A) IHC staining of CD86 in peri-implant tissue at 7 and 14 days. (B) Quantitative analysis of CD86-positive staining areas. (C) H&E staining and (E) Masson's trichrome staining of the bone-implant interface at four weeks. (D) Quantification of new bone formation area based on histological sections. (F) Bone-to-implant contact percentage determined from Masson's trichrome staining. Error bars represent means ± SD for n = 4, ∗ p < 0.05, ∗∗ p < 0.01.
Figure Legend Snippet: In vivo evaluation of osteogenesis and osseointegration. (A) IHC staining of CD86 in peri-implant tissue at 7 and 14 days. (B) Quantitative analysis of CD86-positive staining areas. (C) H&E staining and (E) Masson's trichrome staining of the bone-implant interface at four weeks. (D) Quantification of new bone formation area based on histological sections. (F) Bone-to-implant contact percentage determined from Masson's trichrome staining. Error bars represent means ± SD for n = 4, ∗ p < 0.05, ∗∗ p < 0.01.

Techniques Used: In Vivo, Immunohistochemistry, Staining

Related Articles

Immunofluorescence:

Article Title: Tanshinone IIA accelerates diabetic wound healing via suppression of macrophage NLRP3 inflammasome activity.
Article Snippet: Diabetic foot ulcer (DFU) is a serious complication of diabetes involving persistent inflammation and impaired healing due to neurovascular abnormalities.. Tanshinone IIA (Tan IIA) has been reported to exert antiinflammatory, antioxidant, and vascular protective effects, with potential therapeutic benefits for diabetic complications.. The study aims to investigate the therapeutic effect of Tanshinone IIA on diabetic wound healing and elucidate its underlying mechanisms, with a focus on its modulation of macrophage polarization and inhibition of the NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome.

Staining:

Article Title: Tanshinone IIA accelerates diabetic wound healing via suppression of macrophage NLRP3 inflammasome activity.
Article Snippet: Diabetic foot ulcer (DFU) is a serious complication of diabetes involving persistent inflammation and impaired healing due to neurovascular abnormalities.. Tanshinone IIA (Tan IIA) has been reported to exert antiinflammatory, antioxidant, and vascular protective effects, with potential therapeutic benefits for diabetic complications.. The study aims to investigate the therapeutic effect of Tanshinone IIA on diabetic wound healing and elucidate its underlying mechanisms, with a focus on its modulation of macrophage polarization and inhibition of the NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome.



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( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of <t>CD86</t> expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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Image Search Results


( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Journal: International Journal of Nanomedicine

Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer

doi: 10.2147/IJN.S580749

Figure Lengend Snippet: ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The calreticulin (CRT), CD206, CD86, and phospho-interferon regulatory factor 3 (p-IRF3) antibody were purchased from Proteintech Group, Inc. (Wuhan, China).

Techniques: Immunofluorescence, Expressing, Membrane, Fluorescence, Analysis, Western Blot