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cd86 antibody wl05184  (Wanleibio)

 
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    Structured Review

    Wanleibio cd86 antibody wl05184
    Cd86 Antibody Wl05184, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd86/anti+human+psd95+rabbit/pm42067358-75-0-6
    Average 86 stars, based on 1 article reviews
    cd86 antibody wl05184 - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    SDS Page:

    Article Title: Sertraline can alleviate neuronal and synaptic damage in the hippocampus of PTSD mice and inhibit the increase in myelin.
    Article Snippet: .. After SDS-PAGE electrophoresis, the proteins were transferred to a membrane and blocked with 5% non-fat milk (in TBST) on a shaker at room temperature for 2.5 h. Primary antibodies were incubated overnight at 4 °C, including anti-BDNF antibody (1:1000, WL0168, Wanlei Biotechnology, China), antiSYP antibody (1:1000, WL03058, Wanlei Biotechnology, China), anti-PSD95 antibody (1:1500, WL05046, Wanlei Biotechnology, China), and anti-GAPDH antibody (1:20,000, TA-08, Zhongshan Goldenbridge Biotechnology, China). .. The corresponding secondary antibody (1:20,000, ZB-2306, Zhongshan Goldenbridge Biotechnology, China) was then incubated at room temperature for 1.5 h. After adding ECL solution, the membrane was developed.

    Electrophoresis:

    Article Title: Sertraline can alleviate neuronal and synaptic damage in the hippocampus of PTSD mice and inhibit the increase in myelin.
    Article Snippet: .. After SDS-PAGE electrophoresis, the proteins were transferred to a membrane and blocked with 5% non-fat milk (in TBST) on a shaker at room temperature for 2.5 h. Primary antibodies were incubated overnight at 4 °C, including anti-BDNF antibody (1:1000, WL0168, Wanlei Biotechnology, China), antiSYP antibody (1:1000, WL03058, Wanlei Biotechnology, China), anti-PSD95 antibody (1:1500, WL05046, Wanlei Biotechnology, China), and anti-GAPDH antibody (1:20,000, TA-08, Zhongshan Goldenbridge Biotechnology, China). .. The corresponding secondary antibody (1:20,000, ZB-2306, Zhongshan Goldenbridge Biotechnology, China) was then incubated at room temperature for 1.5 h. After adding ECL solution, the membrane was developed.

    Membrane:

    Article Title: Sertraline can alleviate neuronal and synaptic damage in the hippocampus of PTSD mice and inhibit the increase in myelin.
    Article Snippet: .. After SDS-PAGE electrophoresis, the proteins were transferred to a membrane and blocked with 5% non-fat milk (in TBST) on a shaker at room temperature for 2.5 h. Primary antibodies were incubated overnight at 4 °C, including anti-BDNF antibody (1:1000, WL0168, Wanlei Biotechnology, China), antiSYP antibody (1:1000, WL03058, Wanlei Biotechnology, China), anti-PSD95 antibody (1:1500, WL05046, Wanlei Biotechnology, China), and anti-GAPDH antibody (1:20,000, TA-08, Zhongshan Goldenbridge Biotechnology, China). .. The corresponding secondary antibody (1:20,000, ZB-2306, Zhongshan Goldenbridge Biotechnology, China) was then incubated at room temperature for 1.5 h. After adding ECL solution, the membrane was developed.

    Incubation:

    Article Title: Sertraline can alleviate neuronal and synaptic damage in the hippocampus of PTSD mice and inhibit the increase in myelin.
    Article Snippet: .. After SDS-PAGE electrophoresis, the proteins were transferred to a membrane and blocked with 5% non-fat milk (in TBST) on a shaker at room temperature for 2.5 h. Primary antibodies were incubated overnight at 4 °C, including anti-BDNF antibody (1:1000, WL0168, Wanlei Biotechnology, China), antiSYP antibody (1:1000, WL03058, Wanlei Biotechnology, China), anti-PSD95 antibody (1:1500, WL05046, Wanlei Biotechnology, China), and anti-GAPDH antibody (1:20,000, TA-08, Zhongshan Goldenbridge Biotechnology, China). .. The corresponding secondary antibody (1:20,000, ZB-2306, Zhongshan Goldenbridge Biotechnology, China) was then incubated at room temperature for 1.5 h. After adding ECL solution, the membrane was developed.

    Article Title: Bioengineering an improved three-dimensional vascularized co-culture model for studying Neuron–Microglia interactions
    Article Snippet: Equal amounts of protein were separated by SDSpolyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Massachusetts, USA). .. Membranes were blocked with 5 % non-fat milk in TBST for 1.5 h at room temperature and then incubated overnight at 4 ◦C with the following primary antibodies: Rabbit anti-human Neurofilament Heavy Chain (1:1000, Abcam), Rabbit anti-human BDNF (1:1500, Abcam), Rabbit anti-human OCT4 (1:2000, Abcam), Rabbit anti-human CD86 (1:1000, Wanleibio), Rabbit anti-human CD163 (1:1000, Wanleibio), Rabbit anti-human SDF-1 (1:1000, Wanleibio), Rabbit anti-human PSD95 (1:1000, Wanleibio), Rabbit anti-human GAPDH (1:5000, Servicebio), and Mouse anti-human Beta-Actin (1:5000, Proteintech). .. After washing, membranes were incubated with peroxidase-conjugated secondary antibodies, Goat anti-Mouse IgG (H + L) (1:5000, Proteintech) and HRP-conjugated Goat anti-Rabbit IgG (1:5000, Abbkine, Wuhan, China), for 1.5 h at room temperature.

    western blot:

    Article Title: Multi-Target Inhibitor of ZJQ- 3 F Against AChE/BACE1/GSK3β Targets Improves the Cognitive Impairment of APP/PS1/Tau Triple-Transgenic Mouse Models of Alzheimer's Disease.
    Article Snippet: All tissue samples were subject to western blot analyses of APP (sc- 28365, 1:1000, Santa Cruz, Biotechnology, USA), Tau (WL03184, 1:500, Wanlei Bio, China), P-Tau Ser396 (WL03540, 1:500, Wanlei Bio, China), P-Tau Thr212 (310,208, 1:1000, Zenbio, China), P-Tau Thr181 (12,885, 1:1000, CST, USA), GSK3β (12,456, 1:1000, CST, USA), P-GSK3β-S9 (WL03683, 1:500, Wanlei Bio, China), PSD95 (WL05046, 1:500, Wanlei Bio, China), PSD95 (3450, 1:100, CST, USA), SYP (WL03057, 1:500, Wanlei Bio, China), SYP (36,406, 1:100, CST, USA) SYT (GB111130, 1:500, Servicebio, China).

    other:

    Article Title: Bioengineering an improved three-dimensional vascularized co-culture model for studying Neuron–Microglia interactions
    Article Snippet: Images were captured under a fluorescence microscope imaging system (BZ-X800, Keyence, Osaka, Japan).



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    Image Search Results


    EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Journal: Molecular Biomedicine

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis

    doi: 10.1186/s43556-026-00643-w

    Figure Lengend Snippet: EZH2 inhibition reduced the inflammatory phenotype of macrophages during OA. Macrophage differentiation and M1-like polarization were induced from the THP-1 monocytic cell line ( a ) or from human bone marrow-derived cells isolated from OA patients ( b ) in the presence or absence of EPZ-6438. The expression of pro-inflammatory markers (IL-1β, IL-6 and CXCL1) was analyzed by RT-qPCR. Experiments using THP-1 cells were independently repeated at least three times. Experiments using human bone marrow-derived cells were performed using biological replicates from independent OA patients. Data are presented as mean ± SEM. *: p -value < 0.05; **: p -value < 0.01; ***: p -value < 0.001. Spleens from OA mice described in Fig. and treated with vehicle or EPZ-6438 were collected and analyzed by flow cytometry. Cells were stained for macrophage-associated markers ( c ). Samples were acquired using a FACSVerse flow cytometer and analyzed with FlowJo 7.6.5 software. Results are presented as median ± interquartile range (n = 8 mice). *: p -value < 0.05; **: p -value < 0.01

    Article Snippet: The following antibodies were used for immune cell characterization: CD11b (REA592 VioBlue, 130–113–810, Miltenyi Biotec), F4/80 (REA126 PE-Vio770, 130–118–459, Miltenyi Biotec), CD80 (REA983 APC-Vio770, 130–116–463, Miltenyi Biotec), CD86 (REA1190 PE, 130–122–129, Miltenyi Biotec), MHC-II (REA813 FITC, 130–112–386, Miltenyi Biotec), CD38 (REA616 PerCP-Vio700, 130–109–260, Miltenyi Biotec), and iNOS (REA982 APC, 130–116–423, Miltenyi Biotec).

    Techniques: Inhibition, Derivative Assay, Isolation, Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Software