pcmv cd16a gfp (OriGene)

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OriGene pcmv cd16a gfp
Pcmv Cd16a Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd64+fcgr1/CD64+(FCGR1B)+(NM_001004340)+Human+Tagged+ORF+Clone/pmc11031382-197-0-2
Average 92 stars, based on 1 article reviews

pcmv cd16a gfp - by Bioz Stars, 2026-09

92/100 stars

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Recombinant:

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Maraviroc Sigma Aldrich Cat#PZ0002-25MG Enfuvirtid (T20) Roche N/A AMD3100 Sigma Aldrich Cat#A5602-5MG Efavirenz (EFV) Sigma Aldrich Cat# SML0536-10MG HCMV (AD169 strain) gp34 Kolb et al., 202149 N/A HCMV (AD169 strain) gp68 Kolb et al., 202149 N/A HCMV/AD169 strain) gp34 mtrp Kolb et al., 202149 N/A Human ICOSL Kolb et al., 202149 N/A Human ICAM-1 Kolb et al., 202149 N/A Biotin-XX-conjugated CT-B Thermo Fisher Scientific Cat#C34779 AF647-conjugated CT-B Thermo Fisher Scientific Cat#C34778 AF594-conjugated streptavidin Thermo Fisher Scientific Cat#C34777 Swainsonine Sigma-Aldrich S8195 Kifunensine Sigma-Aldrich K1140 Other EasySep Rosette Human CD4+ T cell enrichment kits STEMCELL Cat#15062 CD4 T cell Isolation Kit Miltenyi Biotech Cat#130-096-533 Human Monocyte Isolation Kit II Miltenyi Biotech Cat#130-091-153 CD14 MicroBeads Miltenyi Biotech Cat#130-050-201 P3 Primary Cell 4D-NucleofectorTM X Kit S LONZA Cat#V4XP-3032 Far Red CellTraceTM Cell Proliferation Kits Thermo Fisher Scientific Cat#C34572 Violet CellTraceTM Cell Proliferation Kits Thermo Fisher Scientific Cat#C34571 Live/deadTM Fixable Yellow Dead Cell Stain Kit Thermo Scientific Cat#L34967 LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit, for 633 or 635 nm excitation Thermo Scientific Cat#L34974 CellTrace CFSE Cell Proliferation Kit Thermo Scientific Cat# C34554 ProLongTM Diamond Antifade Mountant Thermo Scientific Cat# P36990 fixation/permeabilization solution kit BD Cat#554655 Human Cell Surface Marker Screening Panel BD LyoplateTM BD Cat#560747 BD TrucountTM Absolute Counting Tubes BD Cat#340334 CellTiter-Glo 2.0 Promega Cat#G9243 CCF2/AM dye Thermo Fisher Scientific Cat#K1032 PIERCE BCA assay Thermo Fischer Scientific Cat#23225 human albumin/immunoglobulin depletion kit Merck Cat#LSKMAGHDKIT Protein G High Performance Spintrap Merck Cat#GE28-9031-34 goat anti-human IgG (Fc Specific)-agarose antibody Sigma-Aldrich Cat#A3316 Fluoro Brite DMEM Gibco Cat# A18967-01 Experimental models: Cell lines SupT1 DSMZ ACC 140 HEK-293T (293T) DSMZ ACC 635 HeLa ATCC CCL-2 Oligonucleotides See Table S1 for oligo and gRNAs list N/A (Continued on next page) Cell Reports Medicine 5, 101483, April 16, 2024 e4 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pCMV6-XL4-CD32A Origene Cat#SC112914 pCMV6-XL5-CD32B Origene Cat#SC128159 pCMV6-XL5-CD32C Origene Cat#SC124933 pCMV-CD64A-GFP Origene Cat#RG207487 pCMV-CD16A-GFP Origene Cat#RG219204 pBK-CMV-FynN18-GFP O.T. .. Fackler, Heidelberg N/A pGPI-GFP V. Laketa, Heidelberg N/A pUCIP (HCMV AD169 strain) gp34 Kolb et al., 202149 N/A pUCIP (HCMV AD169 strain) gp68 Kolb et al., 202149 N/A pUCIP (HCMV AD169 strain) gp34 mtrp Kolb et al., 202149 N/A pLifeAct-mCherry X. Sewald, Munich N/A pNLENG1-IRES Levy et al., 200479 N/A pNLENG1-I-70 Levy et al., 200479 N/A pR5 HIVivo Horwitz et al., 201780 N/A pX4 HIVivo Albanese et al., 202220 N/A pCMV-BlaM-Vpr Cavrois et al., 200281 N/A pcHIV-1 YFP Barbara M€uller, Heidelberg N/A pcHIV DEnv Barbara M€uller, Heidelberg N/A pVpr-GFP Campbell et al., 200782 N/A Software and algorithms Imaris Viewer Oxford Instruments https://imaris.oxinst.com/imaris-viewer ImageJ National Institutes of Health https://imagej.net/ij/ NIS Elements Nikon https://www.microscope.healthcare.nikon. com/de_EU/products/software/ nis-elements FlowJo BD https://www.flowjo.com/ GraphPad Prism v9.30 GraphPad https://www.graphpad.com/ Adobe Illustrator 2023 Adobe https://www.adobe.com/de Biorender.com Biorender https://www.biorender.com/ Other Corning cell strainer, 40 mM Corning Cat#352340 MACS SmartStrainers, 70 mM Miltenyi Biotec Cat#130-098-462 Stericup-GV 0.22 mm, 500 mL Sigma-Aldrich Cat#SCGVU05RE His-Trap FF crude column GE Healthcare/Cytiva Cat#17525501 (Continued on next page) e5 Cell Reports Medicine 5, 101483, April 16, 2024

Membrane:

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells
Article Snippet: .. pCMV6-XL4-CD32A (Cat. No. SC112914), pCMV6-XL5-CD32B (Cat. No. SC128159), pCMV6-XL5-CD32C (Cat. No. SC124933), pCMV-CD64A-GFP (Cat. No. RG207487) and pCMV-CD16A-GFP (Cat. No. RG219204) were purchase from Origene. pBK-CMV-FynN18-GFP (encoding only the first 18 amino acid of the N-terminal part (membrane domain) and pBK-CMV-LckN18-GFP (encoding only the first 18 amino acid of the N-terminal part (membrane domain) have been reported. pcDNA3.1 (+T7) human CXCR7 and pcDNA3.1 (+T7) human CXCR4 were kindly provided by J. Bernhagen, LMU München. .. To clone plasmids encoding GFP fusion proteins, cDNAs for human CD32A, CD32B, CD32C, CCR5, H2B and SAMHD1 were amplified by PCR and inserted in pEGFP-N1 (Clontech) using Age I and EcoRI .

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells.
Article Snippet: To clone plasmids encoding GFP fusion proteins, cDNAs for human CD32A, CD32B, CD32C, CCR5, H2B and SAMHD1were amplified by PCR and inserted in pEGFP-N1 (Clontech) using AgeI and EcoRI. .. pCMV6-XL4-CD32A (Cat. No. SC112914), pCMV6-XL5-CD32B (Cat. No. SC128159), pCMV6-XL5-CD32C (Cat. No. SC124933), pCMV-CD64A-GFP (Cat. No. RG207487) and pCMV-CD16A-GFP (Cat. No. RG219204) were purchase from Origene. pBK-CMVFynN18-GFP (encoding only the first 18 amino acid of the N-terminal part (membrane domain) and pBK-CMV-LckN18-GFP (encoding only the first 18 amino acid of the N-terminal part (membrane domain) have been reported.85 pcDNA3.1 (+T7) human CXCR7 and pcDNA3.1 (+T7) human CXCR4 were kindly provided by J. Bernhagen, LMUM€unchen. .. To clone plasmids encoding GFP fusion proteins, cDNAs for human CD32A, CD32B, CD32C, CCR5, H2B and SAMHD1were amplified by PCR and inserted in pEGFP-N1 (Clontech) using AgeI and EcoRI.

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