Blocking Assay:Article Title: IGF1R-Targeted Delivery of a Bridged Nucleic Acid Oligonucleotide-Peptide Conjugate for MicroRNA-21 Inhibition in Triple-Negative Breast Cancer
Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with antibodies against PDCD4 protein (ab80590, Abcam), PTEN protein (9552S, Cell Signaling), CD47 protein (AF4670, R&D Systems), PD-L1 protein (501124188, ThermoFisher), PD-L2 protein (501123155, ThermoFisher), Jak2 protein (D2E12, Cell Signaling), and β-actin (AM4302, Ambion), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific), on a Bio-Rad ChemiDoc Imaging System, and analyzed with Image Lab software (Bio-Rad).
Article Title: IGF1R-targeted delivery of a bridged nucleic acid oligonucleotide-peptide conjugate for microRNA-21 inhibition in triple-negative breast cancer
Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4%–12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with rabbit anti-PDCD4 (ab80590, Abcam), sheep anti-CD47 (AF4670, R&D Systems), rabbit anti-PD-L1 (13684S, Cell Signaling), rabbit anti-PD-L2 (ab256386, Abcam), rabbit anti-Jak2 (D2E12, Cell Signaling), and mouse anti-β-actin (AM4302, ThermoFisher), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific) on a Bio-Rad ChemiDoc Imaging System and analyzed with Image Lab software (Bio-Rad).
Incubation:Article Title: IGF1R-Targeted Delivery of a Bridged Nucleic Acid Oligonucleotide-Peptide Conjugate for MicroRNA-21 Inhibition in Triple-Negative Breast Cancer
Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with antibodies against PDCD4 protein (ab80590, Abcam), PTEN protein (9552S, Cell Signaling), CD47 protein (AF4670, R&D Systems), PD-L1 protein (501124188, ThermoFisher), PD-L2 protein (501123155, ThermoFisher), Jak2 protein (D2E12, Cell Signaling), and β-actin (AM4302, Ambion), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific), on a Bio-Rad ChemiDoc Imaging System, and analyzed with Image Lab software (Bio-Rad).
Article Title: Cryoablation for Malignant Bone and Soft Tissue Tumors and Histological Assessment of Ablated Tumors.
Article Snippet: .. Slides were then incubated overnight at room temperature with primary antibodies specific for CD68 (monoclonal mouse anti-CD68, diluted 1:50, catalog M0876; Dako, Glostrup, Denmark), CD4 (monoclonal mouse anti-CD4, diluted 1:50, catalog M7310; Dako), CD8 (monoclonal mouse anti-CD3, diluted 1:50, catalog N1592; Dako), CD16 (polyclonal rabbit anti-CD16, diluted 1:300, catalog 16559-1-AP; Proteintech, Rosemont, IL, USA), CD204 (monoclonal mouse anti-CD204, diluted 1:200, catalog KMUMA01; COSMO BIO Co., Tokyo, Japan), indoleamine 2,3-dioxygenase (IDO) (monoclonal mouse anti-IDO, diluted 1:250, catalog MAB60302; R&D Systems, Minneapolis, MN, USA), and CD47 (polyclonal sheep anti-CD47, diluted 1:200, catalog AF4670; R&D Systems). ..
Article Title: IGF1R-targeted delivery of a bridged nucleic acid oligonucleotide-peptide conjugate for microRNA-21 inhibition in triple-negative breast cancer
Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4%–12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with rabbit anti-PDCD4 (ab80590, Abcam), sheep anti-CD47 (AF4670, R&D Systems), rabbit anti-PD-L1 (13684S, Cell Signaling), rabbit anti-PD-L2 (ab256386, Abcam), rabbit anti-Jak2 (D2E12, Cell Signaling), and mouse anti-β-actin (AM4302, ThermoFisher), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific) on a Bio-Rad ChemiDoc Imaging System and analyzed with Image Lab software (Bio-Rad).
Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer
Article Snippet: A 30-minute incubation with a bovine serum albumin (BSA) protein block (cat. no. A8020; Solarbio, Beijing, China) was conducted to achieve effective blocking. .. Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ). ..
Labeling:Article Title: IGF1R-Targeted Delivery of a Bridged Nucleic Acid Oligonucleotide-Peptide Conjugate for MicroRNA-21 Inhibition in Triple-Negative Breast Cancer
Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with antibodies against PDCD4 protein (ab80590, Abcam), PTEN protein (9552S, Cell Signaling), CD47 protein (AF4670, R&D Systems), PD-L1 protein (501124188, ThermoFisher), PD-L2 protein (501123155, ThermoFisher), Jak2 protein (D2E12, Cell Signaling), and β-actin (AM4302, Ambion), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific), on a Bio-Rad ChemiDoc Imaging System, and analyzed with Image Lab software (Bio-Rad).
Article Title: IGF1R-targeted delivery of a bridged nucleic acid oligonucleotide-peptide conjugate for microRNA-21 inhibition in triple-negative breast cancer
Article Snippet: Lysate protein concentrations were quantified by the Bradford Assay (Bio-Rad). .. Lysate aliquots containing 30 μg protein were separated on NuPAGE 4%–12% Bis-Tris gels (Invitrogen), transferred to PVDF membranes, blocked with blocking buffer, and incubated with rabbit anti-PDCD4 (ab80590, Abcam), sheep anti-CD47 (AF4670, R&D Systems), rabbit anti-PD-L1 (13684S, Cell Signaling), rabbit anti-PD-L2 (ab256386, Abcam), rabbit anti-Jak2 (D2E12, Cell Signaling), and mouse anti-β-actin (AM4302, ThermoFisher), followed by incubation with secondary antibodies labeled with horseradish peroxidase (Invitrogen). .. The resulting protein bands were imaged by luminescence using a SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific) on a Bio-Rad ChemiDoc Imaging System and analyzed with Image Lab software (Bio-Rad).
Western Blot:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Immunohistochemistry:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Flow Cytometry:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Immunofluorescence:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Immunohistochemical staining:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Staining:Article Title: Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47
Article Snippet: .. Supplier anti-human CD47 antibody 1:1000 for WB #AF4670 R&D Systems anti-human CD47 antibody 1:1000 for WB #218810 Abcam anti-human CD47 antibody 1:500 for WB 1:100 for FCM 1:50 for IHC #11-0479-42 Invitrogen anti-mouse CD47 antibody 1:1000 for WB #AF1866 R&D Systems anti-human MAN1B1 antibody 1:500 for WB 1:100 for IHC and IF #sc-393145 Santa Cruz anti-human QPCTL antibody 1:500 for WB #sc-515717 Santa Cruz anti-human MOGS antibody 1:500 for WB #17859-1-AP Proteintech anti-human ERK1/2 antibody 1:1000 for WB #9102 CST anti-human Phospho-ERK1/2 antibody 1:1000 for WB #4370 CST anti-human GANAB antibody 1:1000 for WB #ER63543 Huabio anti-human VINCULIN antibody 1:1000 for WB #ET1705-94 Huabio anti-human β-Actin antibody 1:10000 for WB #HA601037 Huabio anti-human GAPDH antibody 1:1000 for WB #ET1601-4 Huabio anti-FLAG antibody 1:5000 for WB #F1804 Sigma anti-HA antibody 1:1000 for WB #3956 CST anti-HA antibody 1:5000 for WB #26D11 Abmart anti-MYC antibody 1:5000 for WB #19C2 Abmart anti-MYC antibody 1:2000 for WB #R1208-1 Huabio anti-GFP 1:5000 for WB 1:100 for IF #M20004 Abmart anti-human HRD1 1:1000 for WB #13473-1-AP Proteintech anti-human GM130 1:5000 for WB 1:200 for IF #11308-1-AP Proteintech anti-human GRP94 1:2000 for WB 1:200 for IF #60012-2-Ig Proteintech Anti-mouse F4/80 1:200 for IF #GB113373 Servicebio FITC anti-human CD47 antibody 1:100 for FCM #11-0479-42 Invitrogen APC anti-mouse CD47 antibody 1:100 for FCM #17-0471-82 Invitrogen APC anti-mouse/human CD11b Antibody 1:200 for FCM #101212 Biolegend APC/Cyanine7 anti-mouse CD45 Antibody 1:200 for FCM #103116 Biolegend Brilliant Violet 650 anti-mouse F4/80 Antibody 1:200 for FCM #123149 Biolegend PE/Cyanine7 anti-mouse CD80 Antibody 1:200 for FCM #104734 Biolegend Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33223ES60 Yeasen Anti-Human IgG (H+L) Alexa Fluor 1:200 for FCM #33126ES60 Yeasen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32766 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 488 1:500 for IF A32790 Invitrogen Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32773 Invitrogen Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM Plus 555 1:500 for IF A32794 Invitrogen Abbreviations: WB, Western blotting; FCM, flow cytometry; IF, immunofluorescence; 171 IHC, immunohistochemical staining 172 173 174 Supplementary Table S2. ..
Article Title: Histological Assessment of Synovial Sarcoma Before and After TCR-T Cell Therapy and Cryoablation: A Case Report.
Article Snippet: Background/Aim: Cancer/testis antigens (CTAs) are well-known molecular targets with expression restricted to testicular germ cells and malignant tumors.. T-cell receptor (TCR)-engineered T-cell (TCR-T) therapy against CTAs in patients with sarcoma has shown substantial progress, but resistance to TCR-T therapy remains a critical problem.. In this report, we present a case of synovial sarcoma treated with TCR-T therapy targeting the New York-esophageal squamous cell carcinoma (NY-ESO)-1 protein.
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