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cd45 135 conjugated apc  (R&D Systems)


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    Structured Review

    R&D Systems cd45 135 conjugated apc
    Cd45 135 Conjugated Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45/Mouse+CD45+APC-conjugated+Antibody/pm41500514-69-29-32
    Average 93 stars, based on 69 article reviews
    cd45 135 conjugated apc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: The autophagy protein Atg7 is essential for hematopoietic stem cell maintenance
    Article Snippet: Monoclonal rat anti–mouse primary antibodies raised against CD205 (MCA949; clone CC98), CD3 (MCA500G), CD19 (MCA1439), c-kit (2B8), polyclonal rabbit anti–Pax-5 (Abnova), Ly6G/Gr1 (RB6-8C5; eBioscience), and polyclonal rabbit anti-ankyrin (Abcam) were used on frozen sections, with WT mouse lymph node as a positive control. .. Monoclonal rat anti–mouse CD45R (B220; R&D Systems) and polyclonal rabbit anti–mouse/human CD3 (A0452; Dako) were used on formalin-fixed, paraffin-embedded sections, with WT mouse lymph node as a positive control. .. Monoclonal rat anti–mouse primary antibodies raised against CD68 (MCA1957; AbD Serotec), CD11b (M1/70), and polyclonal rabbit anti–mouse serum raised against myeloperoxidase (Ab45977; Abcam) were used on frozen sections, with WT mouse lymph node as a positive control.

    Positive Control:

    Article Title: The autophagy protein Atg7 is essential for hematopoietic stem cell maintenance
    Article Snippet: Monoclonal rat anti–mouse primary antibodies raised against CD205 (MCA949; clone CC98), CD3 (MCA500G), CD19 (MCA1439), c-kit (2B8), polyclonal rabbit anti–Pax-5 (Abnova), Ly6G/Gr1 (RB6-8C5; eBioscience), and polyclonal rabbit anti-ankyrin (Abcam) were used on frozen sections, with WT mouse lymph node as a positive control. .. Monoclonal rat anti–mouse CD45R (B220; R&D Systems) and polyclonal rabbit anti–mouse/human CD3 (A0452; Dako) were used on formalin-fixed, paraffin-embedded sections, with WT mouse lymph node as a positive control. .. Monoclonal rat anti–mouse primary antibodies raised against CD68 (MCA1957; AbD Serotec), CD11b (M1/70), and polyclonal rabbit anti–mouse serum raised against myeloperoxidase (Ab45977; Abcam) were used on frozen sections, with WT mouse lymph node as a positive control.

    Immunofluorescence:

    Article Title: Redox remodeling allows and controls B-cell activation and differentiation.
    Article Snippet: During their differentiation to antibody-secreting plasma cells, B lymphocytes undergo dramatic changes in metabolism, structure, and function.. Here we show that this transition entails extensive intraand extracellular redox changes.. Lipopolysaccharide (LPS)-driven activation and differentiation of naı̈ve murine B splenocytes is paralleled by increased production of reactive oxygen species (ROS) from different sources, followed by a strong antioxidant response.

    Flow Cytometry:

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 10 6 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R&D Systems Inc., Minneapolis, MN, http://www.rndsystems.com ) after being washed in staining buffer for 5 minutes. ..

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. Analysis of the Mesenchymal Nature of HWJSCs To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 10 6 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R&D Systems Inc., Minneapolis, MN, http://www.rndsystems.com ) after being washed in staining buffer for 5 minutes. ..

    Incubation:

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 10 6 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R&D Systems Inc., Minneapolis, MN, http://www.rndsystems.com ) after being washed in staining buffer for 5 minutes. ..

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. Analysis of the Mesenchymal Nature of HWJSCs To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 10 6 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R&D Systems Inc., Minneapolis, MN, http://www.rndsystems.com ) after being washed in staining buffer for 5 minutes. ..

    Article Title: Neonatal inflammation induces lasting sex- and region-dependent microglia activation and sex-dependent impairments in chemoreflexes.
    Article Snippet: Tissue was filtered with cell strainers (40μm) and myelin removed with 130 centrifugation in percoll (23%, GE Healthcare in PBS, 1050xg at 40C for 15 min) to generate single‐cell 131 homogenates. .. 132 Microglia frequency 133 Single cell homogenates were incubated (4°C for 10 min) with antibodies for CD11b conjugated‐134 PE (Miltenyi, 1:25) or CD11b conjugated‐PE‐Vio770 (Miltenyi Biotec, 130‐123‐288, 1:25) and CD45 135 conjugated‐APC (R&D systems, FAB114A, 1:25) in 0.5% BSA, 2mM EDTA, PBS and incubated with DAPI to 136 identify dead cells. ..

    Staining:

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 10 6 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R&D Systems Inc., Minneapolis, MN, http://www.rndsystems.com ) after being washed in staining buffer for 5 minutes. ..

    Article Title: Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration
    Article Snippet: .. Analysis of the Mesenchymal Nature of HWJSCs To confirm the mesenchymal stem cell profile of HWJSCs by flow cytometry, 1 × 10 6 HWJSCs were incubated with allophycocyanin-conjugated CD90 (clone Thy-1A1; mouse IgG2A) and phycoerythrin-conjugated CD45 (clone 2D1; mouse IgG1) antibodies (R&D Systems Inc., Minneapolis, MN, http://www.rndsystems.com ) after being washed in staining buffer for 5 minutes. ..

    Article Title: Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Inhibits Human SARS-CoV-2 and Avian Infectious Bronchitis Coronaviruses
    Article Snippet: .. hUC-MSC cells were detached using 0.25% Trypsin-EDTA and collected by centrifugation at 1000× g for 10 min. Then, 10 6 hWJ-MSCs in 100 μL volume were stained with 10 μL of Peridinium-chlorophyll-protein (Per-CP)-conjugated anti-CD105/Endoglin (Mouse IgG1; Clone 166707, R&D Systems, McKinley Place, MN, USA), Carboxyfluorescein (CFS)-conjugated-CD73 (Mouse IgG2B; Clone 606112, R&D Systems, McKinley Place, MN, USA), allophycocyanin (APC) conjugated anti-CD90/Thy1 (Mouse IgG2A; Clone Thy-1A1, R&D Systems, McKinley Place, MN, USA), phycoerythrin (PE) conjugated anti-CD45 (Mouse IgG1; Clone 2D1, R&D Systems, McKinley Place, MN, USA), and PE-CD34 (Mouse IgG1; Clone QBEnd10, R&D Systems, McKinley Place, MN, USA) monoclonal antibodies for 30 min. ..

    other:

    Article Title: Targeted nanoparticles modify neutrophil function in vivo
    Article Snippet: Antibodies used in this study were the following: rat anti mouse Ly6G-violet 450 (Tonbo Biosciences), rat anti mouse Gr1-FITC (Tonbo Biosciences), rat anti mouse/human CD45-PE (BD Biosciences), rat anti mouse/human CD45-APC (Bio Legend), rat anti mouse/human CD11b-FITC (BD Biosciences), rat anti mouse CD177-Alexa Fluor 647 (R&D Systems), rat anti human CD177-APC (Bio Legend), mouse anti phage M13-PE (Santa Cruz), rabbit anti mouse/human pSmad2 (Cell Signaling), rabbit anti mouse/human pSmad3 (Abcam), rabbit anti mouse/human Smad2/3 (Cell Signaling), Annexin-V FITC (Biotium) and SA-Alexa Fluor 568 (Thermo Scientific).

    Single Cell:

    Article Title: Neonatal inflammation induces lasting sex- and region-dependent microglia activation and sex-dependent impairments in chemoreflexes.
    Article Snippet: Tissue was filtered with cell strainers (40μm) and myelin removed with 130 centrifugation in percoll (23%, GE Healthcare in PBS, 1050xg at 40C for 15 min) to generate single‐cell 131 homogenates. .. 132 Microglia frequency 133 Single cell homogenates were incubated (4°C for 10 min) with antibodies for CD11b conjugated‐134 PE (Miltenyi, 1:25) or CD11b conjugated‐PE‐Vio770 (Miltenyi Biotec, 130‐123‐288, 1:25) and CD45 135 conjugated‐APC (R&D systems, FAB114A, 1:25) in 0.5% BSA, 2mM EDTA, PBS and incubated with DAPI to 136 identify dead cells. ..

    Centrifugation:

    Article Title: Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Inhibits Human SARS-CoV-2 and Avian Infectious Bronchitis Coronaviruses
    Article Snippet: .. hUC-MSC cells were detached using 0.25% Trypsin-EDTA and collected by centrifugation at 1000× g for 10 min. Then, 10 6 hWJ-MSCs in 100 μL volume were stained with 10 μL of Peridinium-chlorophyll-protein (Per-CP)-conjugated anti-CD105/Endoglin (Mouse IgG1; Clone 166707, R&D Systems, McKinley Place, MN, USA), Carboxyfluorescein (CFS)-conjugated-CD73 (Mouse IgG2B; Clone 606112, R&D Systems, McKinley Place, MN, USA), allophycocyanin (APC) conjugated anti-CD90/Thy1 (Mouse IgG2A; Clone Thy-1A1, R&D Systems, McKinley Place, MN, USA), phycoerythrin (PE) conjugated anti-CD45 (Mouse IgG1; Clone 2D1, R&D Systems, McKinley Place, MN, USA), and PE-CD34 (Mouse IgG1; Clone QBEnd10, R&D Systems, McKinley Place, MN, USA) monoclonal antibodies for 30 min. ..

    Bioprocessing:

    Article Title: Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Inhibits Human SARS-CoV-2 and Avian Infectious Bronchitis Coronaviruses
    Article Snippet: .. hUC-MSC cells were detached using 0.25% Trypsin-EDTA and collected by centrifugation at 1000× g for 10 min. Then, 10 6 hWJ-MSCs in 100 μL volume were stained with 10 μL of Peridinium-chlorophyll-protein (Per-CP)-conjugated anti-CD105/Endoglin (Mouse IgG1; Clone 166707, R&D Systems, McKinley Place, MN, USA), Carboxyfluorescein (CFS)-conjugated-CD73 (Mouse IgG2B; Clone 606112, R&D Systems, McKinley Place, MN, USA), allophycocyanin (APC) conjugated anti-CD90/Thy1 (Mouse IgG2A; Clone Thy-1A1, R&D Systems, McKinley Place, MN, USA), phycoerythrin (PE) conjugated anti-CD45 (Mouse IgG1; Clone 2D1, R&D Systems, McKinley Place, MN, USA), and PE-CD34 (Mouse IgG1; Clone QBEnd10, R&D Systems, McKinley Place, MN, USA) monoclonal antibodies for 30 min. ..



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