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r d systems for cd40  (R&D Systems)


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    Structured Review

    R&D Systems r d systems for cd40
    Soluble <t>CD40</t> is the microglia-derived inhibitory factor. (A) Cytokine array analysis reveals 13 factors in the 10-30 kDa molecular weight range that are more highly expressed in MG-spCM with MBP compared to MG - spCM grown in the absence of MBP. The differential intensity was determined by subtracting cytokine intensities of MG-spCM with MBP from MG-spCM minus MBP. ( n = 3 independent experiments) (B) Dose response of mResistin shows inhibition of neurosphere formation at 2000 and 4000 pg/mL ( n = 4 independent experiments, * P < .05, ** P < .01) (C) Dose response of sCD40 shows an inhibitory effect on neurosphere formation at all concentration examined from the spinal cord ( n = 4 independent experiments, **** P < .001). (D) qPCR of CD40 receptor (CD40) and ligand (CD40L) expression in brain and spinal cord derived neurospheres ( n = 3 independent experiments, *** P < .001, * P < .05). (E) Dose response of sCD40 on brain neurosphere formation ( n = 4 independent experiments, **** P < .0001).
    R D Systems For Cd40, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40/Mouse+CD40+Quantikine+ELISA+Kit/pmc11878629-69-7-7
    Average 93 stars, based on 4 article reviews
    r d systems for cd40 - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Microglia in the spinal cord stem cell niche regulate neural precursor cell proliferation via soluble CD40 in response to myelin basic protein"

    Article Title: Microglia in the spinal cord stem cell niche regulate neural precursor cell proliferation via soluble CD40 in response to myelin basic protein

    Journal: Stem Cells

    doi: 10.1093/stmcls/sxae076

    Soluble CD40 is the microglia-derived inhibitory factor. (A) Cytokine array analysis reveals 13 factors in the 10-30 kDa molecular weight range that are more highly expressed in MG-spCM with MBP compared to MG - spCM grown in the absence of MBP. The differential intensity was determined by subtracting cytokine intensities of MG-spCM with MBP from MG-spCM minus MBP. ( n = 3 independent experiments) (B) Dose response of mResistin shows inhibition of neurosphere formation at 2000 and 4000 pg/mL ( n = 4 independent experiments, * P < .05, ** P < .01) (C) Dose response of sCD40 shows an inhibitory effect on neurosphere formation at all concentration examined from the spinal cord ( n = 4 independent experiments, **** P < .001). (D) qPCR of CD40 receptor (CD40) and ligand (CD40L) expression in brain and spinal cord derived neurospheres ( n = 3 independent experiments, *** P < .001, * P < .05). (E) Dose response of sCD40 on brain neurosphere formation ( n = 4 independent experiments, **** P < .0001).
    Figure Legend Snippet: Soluble CD40 is the microglia-derived inhibitory factor. (A) Cytokine array analysis reveals 13 factors in the 10-30 kDa molecular weight range that are more highly expressed in MG-spCM with MBP compared to MG - spCM grown in the absence of MBP. The differential intensity was determined by subtracting cytokine intensities of MG-spCM with MBP from MG-spCM minus MBP. ( n = 3 independent experiments) (B) Dose response of mResistin shows inhibition of neurosphere formation at 2000 and 4000 pg/mL ( n = 4 independent experiments, * P < .05, ** P < .01) (C) Dose response of sCD40 shows an inhibitory effect on neurosphere formation at all concentration examined from the spinal cord ( n = 4 independent experiments, **** P < .001). (D) qPCR of CD40 receptor (CD40) and ligand (CD40L) expression in brain and spinal cord derived neurospheres ( n = 3 independent experiments, *** P < .001, * P < .05). (E) Dose response of sCD40 on brain neurosphere formation ( n = 4 independent experiments, **** P < .0001).

    Techniques Used: Derivative Assay, Molecular Weight, Inhibition, Concentration Assay, Expressing

    Related Articles

    Incubation:

    Article Title: Human platelets stimulate mesangial cells to produce monocyte chemoattractant protein-1 via the CD40/CD40 ligand pathway and may amplify glomerular injury.
    Article Snippet: .. Then, MC were further incubated at final volume of 1.2 ml/well in the presence or absence of various cytokines for 24 h. After 24-h incubation at 37°C, MCP-1 synthesis in supernatants and MC CD40 expression were determined by enzyme-linked immunosorbent assay (ELISA, R&D systems) and FACS analysis, respectively. .. Chemical Inhibitors In the blocking experiment with neutralizing Abs, coculture of MC and unstimulated platelets was carried out at the ratio of 1:100 (MC:platelets) in the presence or absence of 10 g/ml Abs against P-selectin, 1-integrin, CD40L, or IgG1 of irrelevant specificity (Sigma).

    Expressing:

    Article Title: Human platelets stimulate mesangial cells to produce monocyte chemoattractant protein-1 via the CD40/CD40 ligand pathway and may amplify glomerular injury.
    Article Snippet: .. Then, MC were further incubated at final volume of 1.2 ml/well in the presence or absence of various cytokines for 24 h. After 24-h incubation at 37°C, MCP-1 synthesis in supernatants and MC CD40 expression were determined by enzyme-linked immunosorbent assay (ELISA, R&D systems) and FACS analysis, respectively. .. Chemical Inhibitors In the blocking experiment with neutralizing Abs, coculture of MC and unstimulated platelets was carried out at the ratio of 1:100 (MC:platelets) in the presence or absence of 10 g/ml Abs against P-selectin, 1-integrin, CD40L, or IgG1 of irrelevant specificity (Sigma).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Human platelets stimulate mesangial cells to produce monocyte chemoattractant protein-1 via the CD40/CD40 ligand pathway and may amplify glomerular injury.
    Article Snippet: .. Then, MC were further incubated at final volume of 1.2 ml/well in the presence or absence of various cytokines for 24 h. After 24-h incubation at 37°C, MCP-1 synthesis in supernatants and MC CD40 expression were determined by enzyme-linked immunosorbent assay (ELISA, R&D systems) and FACS analysis, respectively. .. Chemical Inhibitors In the blocking experiment with neutralizing Abs, coculture of MC and unstimulated platelets was carried out at the ratio of 1:100 (MC:platelets) in the presence or absence of 10 g/ml Abs against P-selectin, 1-integrin, CD40L, or IgG1 of irrelevant specificity (Sigma).

    FACS:

    Article Title: Human platelets stimulate mesangial cells to produce monocyte chemoattractant protein-1 via the CD40/CD40 ligand pathway and may amplify glomerular injury.
    Article Snippet: .. Then, MC were further incubated at final volume of 1.2 ml/well in the presence or absence of various cytokines for 24 h. After 24-h incubation at 37°C, MCP-1 synthesis in supernatants and MC CD40 expression were determined by enzyme-linked immunosorbent assay (ELISA, R&D systems) and FACS analysis, respectively. .. Chemical Inhibitors In the blocking experiment with neutralizing Abs, coculture of MC and unstimulated platelets was carried out at the ratio of 1:100 (MC:platelets) in the presence or absence of 10 g/ml Abs against P-selectin, 1-integrin, CD40L, or IgG1 of irrelevant specificity (Sigma).

    Cell Culture:

    Article Title: Microglia in the spinal cord stem cell niche regulate neural precursor cell proliferation via soluble CD40 in response to myelin basic protein
    Article Snippet: .. Enzyme-linked immunosorbent assays (ELISAs) were purchased from R&D Systems for CD40 (Catalog #MCCD40) and used for the analysis of microglia-derived CM samples as per the manufacturer's instructions for cell culture supernatant samples. .. Spinal cord and brain neurospheres were collected in Buffer RLT (QIAGEN, Catalog #: 79216) with b-mercaptoethanol.



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