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cd4  (R&D Systems)


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    R&D Systems cd4
    Ectopic lymphoid structures in progressive MS are characterized by infiltration of lymphocytes, FDCs and plasma cells. (A) Parenchyma of FFPE sections of brain and spinal cord of progressive MS patients were screened for infiltrated regions by H&E staining. (B) IF staining for CD3 + T cells and CD20 + B cells on serial sections were used to determine the infiltration score. Score 0, no or <5 lymphocytes; score 1, at least five but <30 lymphocytes; score 2, 31 to 60 lymphocytes; score 3, more than 60 lymphocytes. (C) Meninges and sulci of FFPE sections of brain and spinal cord of progressive MS patients were screened for infiltrated regions by H&E staining. (D) IF staining for CD3 + T cells and CD20 + B cells on serial sections were used to determine the infiltration score. Score 0, no or <5 lymphocytes; score 1, at least five, but <30 lymphocytes; score 2, 31 to 60 lymphocytes; score 3, more than 60 lymphocytes. (E) Whole slides were screened for infiltration on H&E, representative infiltration area depicted in the box (F) , and serial sections were stained, depicted in the box (G–O) . eLFs are characterized by (G) CD3 + T cells and CD20 + B cells, (H) <t>CD4</t> + T cells and CD138 + plasma cells, (I) CD3 + T and CD3 + CD4 + <t>T</t> <t>helper</t> <t>cells</t> (J) Ki67 + proliferating cells, (K) CD35 + and, (L) CD21 + FDCs, (M) CD68 + macrophages as well as (N) BCL-6 + and (O) CXCR5 + GC-like lymphocytes. (P) CD3 + CD8 + cytotoxic T cells as well as some CD3 + CD27 + memory T cells were also present in eLFs. Scale bars (A–D) , (F) indicate 100 μm; (E) indicates 2,000 μm; (G–P) indicate 50 μm.
    Cd4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4/Human+CD4+Antibody/pmc06974514-159-19-21
    Average 94 stars, based on 54 article reviews
    cd4 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Lymphoid Aggregates in the CNS of Progressive Multiple Sclerosis Patients Lack Regulatory T Cells"

    Article Title: Lymphoid Aggregates in the CNS of Progressive Multiple Sclerosis Patients Lack Regulatory T Cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2019.03090

    Ectopic lymphoid structures in progressive MS are characterized by infiltration of lymphocytes, FDCs and plasma cells. (A) Parenchyma of FFPE sections of brain and spinal cord of progressive MS patients were screened for infiltrated regions by H&E staining. (B) IF staining for CD3 + T cells and CD20 + B cells on serial sections were used to determine the infiltration score. Score 0, no or <5 lymphocytes; score 1, at least five but <30 lymphocytes; score 2, 31 to 60 lymphocytes; score 3, more than 60 lymphocytes. (C) Meninges and sulci of FFPE sections of brain and spinal cord of progressive MS patients were screened for infiltrated regions by H&E staining. (D) IF staining for CD3 + T cells and CD20 + B cells on serial sections were used to determine the infiltration score. Score 0, no or <5 lymphocytes; score 1, at least five, but <30 lymphocytes; score 2, 31 to 60 lymphocytes; score 3, more than 60 lymphocytes. (E) Whole slides were screened for infiltration on H&E, representative infiltration area depicted in the box (F) , and serial sections were stained, depicted in the box (G–O) . eLFs are characterized by (G) CD3 + T cells and CD20 + B cells, (H) CD4 + T cells and CD138 + plasma cells, (I) CD3 + T and CD3 + CD4 + T helper cells (J) Ki67 + proliferating cells, (K) CD35 + and, (L) CD21 + FDCs, (M) CD68 + macrophages as well as (N) BCL-6 + and (O) CXCR5 + GC-like lymphocytes. (P) CD3 + CD8 + cytotoxic T cells as well as some CD3 + CD27 + memory T cells were also present in eLFs. Scale bars (A–D) , (F) indicate 100 μm; (E) indicates 2,000 μm; (G–P) indicate 50 μm.
    Figure Legend Snippet: Ectopic lymphoid structures in progressive MS are characterized by infiltration of lymphocytes, FDCs and plasma cells. (A) Parenchyma of FFPE sections of brain and spinal cord of progressive MS patients were screened for infiltrated regions by H&E staining. (B) IF staining for CD3 + T cells and CD20 + B cells on serial sections were used to determine the infiltration score. Score 0, no or <5 lymphocytes; score 1, at least five but <30 lymphocytes; score 2, 31 to 60 lymphocytes; score 3, more than 60 lymphocytes. (C) Meninges and sulci of FFPE sections of brain and spinal cord of progressive MS patients were screened for infiltrated regions by H&E staining. (D) IF staining for CD3 + T cells and CD20 + B cells on serial sections were used to determine the infiltration score. Score 0, no or <5 lymphocytes; score 1, at least five, but <30 lymphocytes; score 2, 31 to 60 lymphocytes; score 3, more than 60 lymphocytes. (E) Whole slides were screened for infiltration on H&E, representative infiltration area depicted in the box (F) , and serial sections were stained, depicted in the box (G–O) . eLFs are characterized by (G) CD3 + T cells and CD20 + B cells, (H) CD4 + T cells and CD138 + plasma cells, (I) CD3 + T and CD3 + CD4 + T helper cells (J) Ki67 + proliferating cells, (K) CD35 + and, (L) CD21 + FDCs, (M) CD68 + macrophages as well as (N) BCL-6 + and (O) CXCR5 + GC-like lymphocytes. (P) CD3 + CD8 + cytotoxic T cells as well as some CD3 + CD27 + memory T cells were also present in eLFs. Scale bars (A–D) , (F) indicate 100 μm; (E) indicates 2,000 μm; (G–P) indicate 50 μm.

    Techniques Used: Clinical Proteomics, Staining

    Follicle-like structures of SPMS brains exhibit CD3 + CD4 + T cells, which neither express PD-1 nor FOXP3. (A–E) IF staining of CD3, CD4 and PD-1 reveal CD3 + CD4 + PD-1 − T-helper cells in progressive MS. Inserts in the upper right corners show magnification of the white box. (F–I) IF staining of CD3 and FOXP3 on serial sections of a representative meningeal follicle-like structure in SPMS (same region as ). CD3 + T cells, but no FOXP3 + cells were detected. Inserts show magnification of the white box. Scale bars indicate 100 μm, inserts 10 μm.
    Figure Legend Snippet: Follicle-like structures of SPMS brains exhibit CD3 + CD4 + T cells, which neither express PD-1 nor FOXP3. (A–E) IF staining of CD3, CD4 and PD-1 reveal CD3 + CD4 + PD-1 − T-helper cells in progressive MS. Inserts in the upper right corners show magnification of the white box. (F–I) IF staining of CD3 and FOXP3 on serial sections of a representative meningeal follicle-like structure in SPMS (same region as ). CD3 + T cells, but no FOXP3 + cells were detected. Inserts show magnification of the white box. Scale bars indicate 100 μm, inserts 10 μm.

    Techniques Used: Staining

    CD4 + CXCR5 + T FH s mark positive for cytoplasmic NFATc1. (A–E) Consecutive IF staining of CD4, CXCR5 and NFATc1 on serial sections of follicle-like structures in SPMS (same region as , ). Inserts show magnification of the white box. (F) NFATc1 appears to be cytoplasmic in MS brains, compared to nuclear localization within tonsillar GCs (left insert) and cytoplasmic predominance in inter-follicular cells (right insert). Scale bars indicate 100 μm, inserts 10 μm.
    Figure Legend Snippet: CD4 + CXCR5 + T FH s mark positive for cytoplasmic NFATc1. (A–E) Consecutive IF staining of CD4, CXCR5 and NFATc1 on serial sections of follicle-like structures in SPMS (same region as , ). Inserts show magnification of the white box. (F) NFATc1 appears to be cytoplasmic in MS brains, compared to nuclear localization within tonsillar GCs (left insert) and cytoplasmic predominance in inter-follicular cells (right insert). Scale bars indicate 100 μm, inserts 10 μm.

    Techniques Used: Staining

    B cells enrich in lymphoid aggregates. (A) Absolute number of infiltrates that were positive for T FH in follicle-like structures (F+) and less defined infiltrates (F-). Fisher's exact test, N = 76, X 2 (1) = 4.55, p = 0.048, d = 0.505. (B) Mean percentage of T FH cells defined as CD4 + CXCR5 + cells of CD4 + cells in two serial FFPE sections of follicle-like structures (F+) and less defined infiltrates (F-) in SPMS brains and spinal cords. F-, M = 15.57, SD = 17.13, n = 39; F+, M = 17.04, SD = 15.85, n = 37. Mann Whitney test, U = 635.0, p = 0.369. (C) CD20/CD3 ratio in follicle-like structures (F+) and less defined infiltrates (F-) based on IF co-staining of CD3 and CD20. F-, M = 0.28, SD = 0.33, n = 39; F+, M = 0.38, SD = 0.34, n = 37; Mann Whitney test, U = 525.5, p = 0.042. * p < 0.05.
    Figure Legend Snippet: B cells enrich in lymphoid aggregates. (A) Absolute number of infiltrates that were positive for T FH in follicle-like structures (F+) and less defined infiltrates (F-). Fisher's exact test, N = 76, X 2 (1) = 4.55, p = 0.048, d = 0.505. (B) Mean percentage of T FH cells defined as CD4 + CXCR5 + cells of CD4 + cells in two serial FFPE sections of follicle-like structures (F+) and less defined infiltrates (F-) in SPMS brains and spinal cords. F-, M = 15.57, SD = 17.13, n = 39; F+, M = 17.04, SD = 15.85, n = 37. Mann Whitney test, U = 635.0, p = 0.369. (C) CD20/CD3 ratio in follicle-like structures (F+) and less defined infiltrates (F-) based on IF co-staining of CD3 and CD20. F-, M = 0.28, SD = 0.33, n = 39; F+, M = 0.38, SD = 0.34, n = 37; Mann Whitney test, U = 525.5, p = 0.042. * p < 0.05.

    Techniques Used: MANN-WHITNEY, Staining

    eLFs of brain and spinal cord exhibit more CD4 + CD69 + cells. (A–D) Consecutive IF co-staining of CD4 and CD69 in follicle-like structures of SPMS brains and spinal cords. Inserts show co-localization of CD4 + cells with CD69 suggesting tissue-resident T cells in a representative meningeal eLF of SPMS spinal cord (same region as , , ). Scale bar indicate 100 μm, scale bars of the inserts indicate 10 μm. (E) Percentage of tissue-resident cells defined as CD4 + CD69 + cells of CD4 + cells in follicle-like structures (F+) and less defined infiltrates (F-) in SPMS brains and spinal cords. F-, 5.70, SD = 10.67, n = 38; F+, M = 7.92, SD = 9.39, n = 32; Mann Whitney test, U = 434.0, p = 0.028.
    Figure Legend Snippet: eLFs of brain and spinal cord exhibit more CD4 + CD69 + cells. (A–D) Consecutive IF co-staining of CD4 and CD69 in follicle-like structures of SPMS brains and spinal cords. Inserts show co-localization of CD4 + cells with CD69 suggesting tissue-resident T cells in a representative meningeal eLF of SPMS spinal cord (same region as , , ). Scale bar indicate 100 μm, scale bars of the inserts indicate 10 μm. (E) Percentage of tissue-resident cells defined as CD4 + CD69 + cells of CD4 + cells in follicle-like structures (F+) and less defined infiltrates (F-) in SPMS brains and spinal cords. F-, 5.70, SD = 10.67, n = 38; F+, M = 7.92, SD = 9.39, n = 32; Mann Whitney test, U = 434.0, p = 0.028.

    Techniques Used: Staining, MANN-WHITNEY

    Related Articles

    Lysis:

    Article Title: A cell line model for the study of CD4-negative HIV-1 infection and latent virus reservoirs
    Article Snippet: PrePrint: ion assay (RIPA) lysis buffer and loaded onto a 10% SDS-PAGE gel. The gel was transferred to nitrocellulose membrane and probed with polyclonal goat anti-human CD4 primary antibody (Novus Biologicals, R&D Systems #AF-379-NA, Lot #YS0819111) and protein visualized using rabbit anti-goat HRP (Novus Biologicals) and enhanced chemiluminescence. Cell surface expression of CD4, CXCR4, and CCR5 was assess

    Cytometry:

    Article Title: A cell line model for the study of CD4-negative HIV-1 infection and latent virus reservoirs
    Article Snippet: PrePrint: ion assay (RIPA) lysis buffer and loaded onto a 10% SDS-PAGE gel. The gel was transferred to nitrocellulose membrane and probed with polyclonal goat anti-human CD4 primary antibody (Novus Biologicals, R&D Systems #AF-379-NA, Lot #YS0819111) and protein visualized using rabbit anti-goat HRP (Novus Biologicals) and enhanced chemiluminescence. Cell surface expression of CD4, CXCR4, and CCR5 was assess

    Flow Cytometry:

    Article Title: A cell line model for the study of CD4-negative HIV-1 infection and latent virus reservoirs
    Article Snippet: PrePrint: ion assay (RIPA) lysis buffer and loaded onto a 10% SDS-PAGE gel. The gel was transferred to nitrocellulose membrane and probed with polyclonal goat anti-human CD4 primary antibody (Novus Biologicals, R&D Systems #AF-379-NA, Lot #YS0819111) and protein visualized using rabbit anti-goat HRP (Novus Biologicals) and enhanced chemiluminescence. Cell surface expression of CD4, CXCR4, and CCR5 was assess

    SDS Page:

    Article Title: A cell line model for the study of CD4-negative HIV-1 infection and latent virus reservoirs
    Article Snippet: PrePrint: ion assay (RIPA) lysis buffer and loaded onto a 10% SDS-PAGE gel. The gel was transferred to nitrocellulose membrane and probed with polyclonal goat anti-human CD4 primary antibody (Novus Biologicals, R&D Systems #AF-379-NA, Lot #YS0819111) and protein visualized using rabbit anti-goat HRP (Novus Biologicals) and enhanced chemiluminescence. Cell surface expression of CD4, CXCR4, and CCR5 was assess

    Expressing:

    Article Title: A cell line model for the study of CD4-negative HIV-1 infection and latent virus reservoirs
    Article Snippet: PrePrint: ion assay (RIPA) lysis buffer and loaded onto a 10% SDS-PAGE gel. The gel was transferred to nitrocellulose membrane and probed with polyclonal goat anti-human CD4 primary antibody (Novus Biologicals, R&D Systems #AF-379-NA, Lot #YS0819111) and protein visualized using rabbit anti-goat HRP (Novus Biologicals) and enhanced chemiluminescence. Cell surface expression of CD4, CXCR4, and CCR5 was assess

    Membrane:

    Article Title: A cell line model for the study of CD4-negative HIV-1 infection and latent virus reservoirs
    Article Snippet: PrePrint: ion assay (RIPA) lysis buffer and loaded onto a 10% SDS-PAGE gel. The gel was transferred to nitrocellulose membrane and probed with polyclonal goat anti-human CD4 primary antibody (Novus Biologicals, R&D Systems #AF-379-NA, Lot #YS0819111) and protein visualized using rabbit anti-goat HRP (Novus Biologicals) and enhanced chemiluminescence. Cell surface expression of CD4, CXCR4, and CCR5 was assess

    Radio Immunoprecipitation:

    Article Title: A cell line model for the study of CD4-negative HIV-1 infection and latent virus reservoirs
    Article Snippet: PrePrint: ion assay (RIPA) lysis buffer and loaded onto a 10% SDS-PAGE gel. The gel was transferred to nitrocellulose membrane and probed with polyclonal goat anti-human CD4 primary antibody (Novus Biologicals, R&D Systems #AF-379-NA, Lot #YS0819111) and protein visualized using rabbit anti-goat HRP (Novus Biologicals) and enhanced chemiluminescence. Cell surface expression of CD4, CXCR4, and CCR5 was assess



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    Article Title: Rare Feline Gingival Extramedullary Plasmacytoma: Clinical Application of Electrochemotherapy for Local Control

    doi: 10.1002/vms3.71294

    Figure Lengend Snippet: Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized lymphocytes, with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.

    Article Snippet: For flow cytometric analysis, antibodies against CD21 (B‐cell marker; clone CA2.1D6, RPE‐conjugated, Bio‐Rad Antibodies, MCA1781R), CD5 (pan–T‐cell marker; clone FE1.1B11, FITC‐conjugated, Bio‐Rad Antibodies, MCA2038F), CD4 (helper T‐cell marker; clone VPG34, FITC‐conjugated; Bio‐Rad Antibodies, MCA1346F) and CD8 (cytotoxic T‐cell marker; clone VPG9, RPE‐conjugated; Bio‐Rad Antibodies, MCA1347R) were used.

    Techniques: Immunohistochemical staining, Staining, Expressing