b6 cg  (Jackson Laboratory)

Journal: Veterinary Medicine and Science

Article Title: Rare Feline Gingival Extramedullary Plasmacytoma: Clinical Application of Electrochemotherapy for Local Control

doi: 10.1002/vms3.71294

Figure Lengend Snippet: Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized lymphocytes, with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.

Article Snippet: For flow cytometric analysis, antibodies against CD21 (B‐cell marker; clone CA2.1D6, RPE‐conjugated, Bio‐Rad Antibodies, MCA1781R), CD5 (pan–T‐cell marker; clone FE1.1B11, FITC‐conjugated, Bio‐Rad Antibodies, MCA2038F), CD4 (helper T‐cell marker; clone VPG34, FITC‐conjugated; Bio‐Rad Antibodies, MCA1346F) and CD8 (cytotoxic T‐cell marker; clone VPG9, RPE‐conjugated; Bio‐Rad Antibodies, MCA1347R) were used.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: FEBS Open Bio

Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

doi: 10.1002/2211-5463.70349

Figure Lengend Snippet: (A) Representative dot plots of isolated peripheral blood mononuclear cells (PBMCs) before (upper row) and after (lower row) total CD4 + cells magnetic‐activated cell sorting (MACS) from a young donor. Cells are gated on the living population and checked for CD4 vs. CD8 (left panels), CD45RA vs. CD28 expression (middle panels), and for CD127 vs. CD25 (right panels) expression. Frequencies of CD4 + Foxp3 + (B) and CD25 + of CD4 + Foxp3 + (C) cells are shown after 4 days of in vitro induction of Treg cells in the presence of 10% fetal calf serum (FCS) containing medium RPMI or in serum‐free medium XVivo‐15 (MEAN; n = 2, young donors).

Article Snippet: For the purification of total CD4 + T cells, we used the Miltenyi Biotec CD4 T‐cell Kit (130‐096‐533) according to the manufacturer's protocol.

Techniques: Isolation, FACS, Expressing, In Vitro

Journal: FEBS Open Bio

Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

doi: 10.1002/2211-5463.70349

Figure Lengend Snippet: Frequency of Foxp3 + CD4 + cells (A) and CD25 expression in Foxp3 + cells (B) after 6 days of induction in TCR‐stimulated cells in the presence of IL‐2 (iTreg IL‐2 ) only or IL‐2 + TGF‐β (iTreg TGFβ ) in young and older individuals. (Mean ± SEM; n ≥ 3). Statistical significance P < 0.05*, and P < 0.01** (two‐way ANOVA with Sidak's post hoc test).

Article Snippet: For the purification of total CD4 + T cells, we used the Miltenyi Biotec CD4 T‐cell Kit (130‐096‐533) according to the manufacturer's protocol.

Techniques: Expressing