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memory cd4 t cells  (Miltenyi Biotec)


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    Miltenyi Biotec memory cd4 t cells
    Memory Cd4 T Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+kit/Memory+CD4%2B+T+Cell+Isolation+Kit%2C+human/pmc12208330-215-2-9
    Average 95 stars, based on 36 article reviews
    memory cd4 t cells - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Isolation:

    Article Title: L-Phenylalanine is a metabolic checkpoint of human Th2 cells
    Article Snippet: CD4+T cell Isolation Kit, human , Miltenyi Biotec , Cat#130-096-533. .. Memory CD4+T cell Isolation Kit, human , Miltenyi Biotec , Cat#130-091-893. .. Seahorse Glycolytic Rate Assay kit , Agilent Technologies , Cat#103344-100.

    Article Title: Post-COVID impairment of memory T cell responses to community-acquired pathogens can be rectified by activating cellular metabolism
    Article Snippet: .. CD4+CD45RO+ memory T cells were isolated by negative selection using the CD4 memory T cell isolation kit from Miltenyi Biotec. .. Cells were lysed and RNA extracted using the RNEasy Mini Kit (Qiagen).

    Article Title: O-GlcNAcylation and low glycolysis underpin Th2 polarization by dendritic cells
    Article Snippet: Naïve CD4 T cells for assessment of DC capacity for Th1/2 polarization were isolated from allogenic buffy coat PBMCs using a naïve human CD4 T cell isolation kit (#480042, BioLegend). .. Memory CD4 T cells for assessment of DC capacity for Th17 reactivation were isolated using a human CD4 T cell isolation kit (#130-096-533, Miltenyi) followed by a combination of anti-CD45RO-PE (UCHL1 clone; #R0843, Dako) and anti-PE microbreads (#130-048-801) to eliminate naïve CD4 T cells. .. For Th1/2 polarization, 5.000 DCs that were stimulated for 24h were co-cultured with 20.000 allogenic naïve CD4 T cells in the presence of 20 pg/mL Staphylococcal Enterotoxin B (SEB; #S4881, Sigma) in a cell culture treated flat-bottom 96 well plate.

    Article Title: Rationale for multi-epitope TGFβ vaccination in pancreatic cancer: evidence from immunologic and clinical correlates
    Article Snippet: Fc receptors were blocked for 10 min, followed by 30 min staining with HLA-DR,DP,DQ-FITC (BD Biosciences) and NIR viability dye. .. CD4+CD45RO+ memory T cells were enriched using the human Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec). .. CD14+ monocytes were isolated from PBMCs using MACS CD14 MicroBeads and pulsed with peptide (10 nM) for 2 h. CD4+CD45RO+ memory T cells were isolated from the CD14 − fraction and co-cultured with peptide-pulsed monocytes.

    Article Title: Refined single-cell profiling captures a CCR5 high CD4 + cytotoxic T-cell precursor in multiple sclerosis.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation with Ficoll–Paque (GE Healthcare). .. Memory CD4+ T cells were subsequently purified from PBMCs through magnetic-activated cell sorting (MACS) utilising the Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec, Cat130-091-893) on the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol. .. MACS isolated CD4+ memory cells were cryopreserved in RPMI 1640 medium (Gibco, Cat# 11875093) supplemented with 20% calf serum (FCS; Gibco, Cat# 10270-106) and 10% dimethyl sulfoxide (Sigma–Aldrich), and stored at −80 ◦C until further use in experiments.

    Article Title: Rationale for multi-epitope TGFβ vaccination in pancreatic cancer: evidence from immunologic and clinical correlates.
    Article Snippet: Fc receptors were blocked for 10 min, followed by 30min staining with HLA-DR,DP,DQ-FITC (BD Biosciences) and NIR viability dye. .. Isolation and identification of circulating memory CD4+ T cells specific to TGFβ-33 CD4+CD45RO+ memory T cells were enriched using the human Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec).42 CD14+ monocytes were isolated from PBMCs using MACS CD14 MicroBeads16 and pulsed with peptide (10 nM) for 2 h. CD4+CD45RO+ memory T cells were isolated from the CD14− fraction and cocultured with peptide-pulsed monocytes. ..

    Article Title: L-Phenylalanine is a metabolic checkpoint of human Th2 cells
    Article Snippet: .. Next, total CD3 + CD4 + T cells or memory CD3 + CD4 + T cells were isolated using the human CD4 + T cell Isolation Kit or Memory CD4 + T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), for respective cell types, according to manufacturer instructions on the autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Isolated cells were then transferred to RPMI1640 medium (Lonza, Basel, Switzerland) with 10% heat inactivated FCS (Merck, Darmstadt, Germany), 1% NEA (Merck, Darmstadt, Germany) and 100U/mL rhIL-2 (PeproTech, Thermo Fisher Scientific, Waltham, MA, USA) (R10+IL2).

    Isolation:

    Article Title: Post-COVID impairment of memory T cell responses to community-acquired pathogens can be rectified by activating cellular metabolism
    Article Snippet: .. CD4+CD45RO+ memory T cells were isolated by negative selection using the CD4 memory T cell isolation kit from Miltenyi Biotec. .. Cells were lysed and RNA extracted using the RNEasy Mini Kit (Qiagen).

    Article Title: O-GlcNAcylation and low glycolysis underpin Th2 polarization by dendritic cells
    Article Snippet: Naïve CD4 T cells for assessment of DC capacity for Th1/2 polarization were isolated from allogenic buffy coat PBMCs using a naïve human CD4 T cell isolation kit (#480042, BioLegend). .. Memory CD4 T cells for assessment of DC capacity for Th17 reactivation were isolated using a human CD4 T cell isolation kit (#130-096-533, Miltenyi) followed by a combination of anti-CD45RO-PE (UCHL1 clone; #R0843, Dako) and anti-PE microbreads (#130-048-801) to eliminate naïve CD4 T cells. .. For Th1/2 polarization, 5.000 DCs that were stimulated for 24h were co-cultured with 20.000 allogenic naïve CD4 T cells in the presence of 20 pg/mL Staphylococcal Enterotoxin B (SEB; #S4881, Sigma) in a cell culture treated flat-bottom 96 well plate.

    Article Title: Rationale for multi-epitope TGFβ vaccination in pancreatic cancer: evidence from immunologic and clinical correlates.
    Article Snippet: Fc receptors were blocked for 10 min, followed by 30min staining with HLA-DR,DP,DQ-FITC (BD Biosciences) and NIR viability dye. .. Isolation and identification of circulating memory CD4+ T cells specific to TGFβ-33 CD4+CD45RO+ memory T cells were enriched using the human Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec).42 CD14+ monocytes were isolated from PBMCs using MACS CD14 MicroBeads16 and pulsed with peptide (10 nM) for 2 h. CD4+CD45RO+ memory T cells were isolated from the CD14− fraction and cocultured with peptide-pulsed monocytes. ..

    Article Title: L-Phenylalanine is a metabolic checkpoint of human Th2 cells
    Article Snippet: .. Next, total CD3 + CD4 + T cells or memory CD3 + CD4 + T cells were isolated using the human CD4 + T cell Isolation Kit or Memory CD4 + T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), for respective cell types, according to manufacturer instructions on the autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Isolated cells were then transferred to RPMI1640 medium (Lonza, Basel, Switzerland) with 10% heat inactivated FCS (Merck, Darmstadt, Germany), 1% NEA (Merck, Darmstadt, Germany) and 100U/mL rhIL-2 (PeproTech, Thermo Fisher Scientific, Waltham, MA, USA) (R10+IL2).

    Selection:

    Article Title: Post-COVID impairment of memory T cell responses to community-acquired pathogens can be rectified by activating cellular metabolism
    Article Snippet: .. CD4+CD45RO+ memory T cells were isolated by negative selection using the CD4 memory T cell isolation kit from Miltenyi Biotec. .. Cells were lysed and RNA extracted using the RNEasy Mini Kit (Qiagen).

    Purification:

    Article Title: Refined single-cell profiling captures a CCR5 high CD4 + cytotoxic T-cell precursor in multiple sclerosis.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation with Ficoll–Paque (GE Healthcare). .. Memory CD4+ T cells were subsequently purified from PBMCs through magnetic-activated cell sorting (MACS) utilising the Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec, Cat130-091-893) on the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol. .. MACS isolated CD4+ memory cells were cryopreserved in RPMI 1640 medium (Gibco, Cat# 11875093) supplemented with 20% calf serum (FCS; Gibco, Cat# 10270-106) and 10% dimethyl sulfoxide (Sigma–Aldrich), and stored at −80 ◦C until further use in experiments.

    FACS:

    Article Title: Refined single-cell profiling captures a CCR5 high CD4 + cytotoxic T-cell precursor in multiple sclerosis.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation with Ficoll–Paque (GE Healthcare). .. Memory CD4+ T cells were subsequently purified from PBMCs through magnetic-activated cell sorting (MACS) utilising the Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec, Cat130-091-893) on the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol. .. MACS isolated CD4+ memory cells were cryopreserved in RPMI 1640 medium (Gibco, Cat# 11875093) supplemented with 20% calf serum (FCS; Gibco, Cat# 10270-106) and 10% dimethyl sulfoxide (Sigma–Aldrich), and stored at −80 ◦C until further use in experiments.

    Magnetic Cell Separation:

    Article Title: Refined single-cell profiling captures a CCR5 high CD4 + cytotoxic T-cell precursor in multiple sclerosis.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation with Ficoll–Paque (GE Healthcare). .. Memory CD4+ T cells were subsequently purified from PBMCs through magnetic-activated cell sorting (MACS) utilising the Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec, Cat130-091-893) on the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol. .. MACS isolated CD4+ memory cells were cryopreserved in RPMI 1640 medium (Gibco, Cat# 11875093) supplemented with 20% calf serum (FCS; Gibco, Cat# 10270-106) and 10% dimethyl sulfoxide (Sigma–Aldrich), and stored at −80 ◦C until further use in experiments.

    Article Title: Rationale for multi-epitope TGFβ vaccination in pancreatic cancer: evidence from immunologic and clinical correlates.
    Article Snippet: Fc receptors were blocked for 10 min, followed by 30min staining with HLA-DR,DP,DQ-FITC (BD Biosciences) and NIR viability dye. .. Isolation and identification of circulating memory CD4+ T cells specific to TGFβ-33 CD4+CD45RO+ memory T cells were enriched using the human Memory CD4+ T Cell Isolation Kit (Miltenyi Biotec).42 CD14+ monocytes were isolated from PBMCs using MACS CD14 MicroBeads16 and pulsed with peptide (10 nM) for 2 h. CD4+CD45RO+ memory T cells were isolated from the CD14− fraction and cocultured with peptide-pulsed monocytes. ..



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    Image Search Results


    (A) Representative dot plots of isolated peripheral blood mononuclear cells (PBMCs) before (upper row) and after (lower row) total CD4 + cells magnetic‐activated cell sorting (MACS) from a young donor. Cells are gated on the living population and checked for CD4 vs. CD8 (left panels), CD45RA vs. CD28 expression (middle panels), and for CD127 vs. CD25 (right panels) expression. Frequencies of CD4 + Foxp3 + (B) and CD25 + of CD4 + Foxp3 + (C) cells are shown after 4 days of in vitro induction of Treg cells in the presence of 10% fetal calf serum (FCS) containing medium RPMI or in serum‐free medium XVivo‐15 (MEAN; n = 2, young donors).

    Journal: FEBS Open Bio

    Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

    doi: 10.1002/2211-5463.70349

    Figure Lengend Snippet: (A) Representative dot plots of isolated peripheral blood mononuclear cells (PBMCs) before (upper row) and after (lower row) total CD4 + cells magnetic‐activated cell sorting (MACS) from a young donor. Cells are gated on the living population and checked for CD4 vs. CD8 (left panels), CD45RA vs. CD28 expression (middle panels), and for CD127 vs. CD25 (right panels) expression. Frequencies of CD4 + Foxp3 + (B) and CD25 + of CD4 + Foxp3 + (C) cells are shown after 4 days of in vitro induction of Treg cells in the presence of 10% fetal calf serum (FCS) containing medium RPMI or in serum‐free medium XVivo‐15 (MEAN; n = 2, young donors).

    Article Snippet: For the purification of total CD4 + T cells, we used the Miltenyi Biotec CD4 T‐cell Kit (130‐096‐533) according to the manufacturer's protocol.

    Techniques: Isolation, FACS, Expressing, In Vitro

    Frequency of Foxp3 + CD4 + cells (A) and CD25 expression in Foxp3 + cells (B) after 6 days of induction in TCR‐stimulated cells in the presence of IL‐2 (iTreg IL‐2 ) only or IL‐2 + TGF‐β (iTreg TGFβ ) in young and older individuals. (Mean ± SEM; n ≥ 3). Statistical significance P < 0.05*, and P < 0.01** (two‐way ANOVA with Sidak's post hoc test).

    Journal: FEBS Open Bio

    Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

    doi: 10.1002/2211-5463.70349

    Figure Lengend Snippet: Frequency of Foxp3 + CD4 + cells (A) and CD25 expression in Foxp3 + cells (B) after 6 days of induction in TCR‐stimulated cells in the presence of IL‐2 (iTreg IL‐2 ) only or IL‐2 + TGF‐β (iTreg TGFβ ) in young and older individuals. (Mean ± SEM; n ≥ 3). Statistical significance P < 0.05*, and P < 0.01** (two‐way ANOVA with Sidak's post hoc test).

    Article Snippet: For the purification of total CD4 + T cells, we used the Miltenyi Biotec CD4 T‐cell Kit (130‐096‐533) according to the manufacturer's protocol.

    Techniques: Expressing

    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Control, Lysis, Flow Cytometry, Staining, In Vitro, Isolation, Incubation, Expressing, Comparison

    PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Analysis, Activation Assay, Immunohistochemistry, Expressing

    PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Tissue, Microinjection, Injection, Staining, Analysis, Expressing, Activity Assay