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mouse t cell isolation kit  (R&D Systems)


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    Structured Review

    R&D Systems mouse t cell isolation kit
    Mouse T Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+kit/MagCellect+Mouse+CD4%2B+T+Cell+Isolation+Kit/pmc04458448-182-33-38
    Average 91 stars, based on 23 article reviews
    mouse t cell isolation kit - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Selection:

    Article Title: Defining genetic variation in widely used congenic and backcrossed mouse models reveals varied regulation of genes important for immune responses
    Article Snippet: .. Negative selection kits (CD4 + T cell MAGM202; or CD8 + T cell MAGM203) from R&D systems were used for the T cell isolations. ..

    Article Title: Hypercholesterolemic Dysregulation of Calpain in Lymphatic Endothelial Cells Interferes With Regulatory T-Cell Stability and Trafficking
    Article Snippet: .. Then the cells were labeled by using a MagCellect Mouse CD4+ T-Cell Isolation Kit (R&D Systems, Inc) and were separated by magnetic fields using negative selection. .. To obtain Tregs, CD4+ cells were labeled by using MagCellect Mouse CD4+CD25+ Regulatory T-Cell Isolation Kit (R&D Systems, Inc), and were separated by magnetic fields using positive selection.

    Isolation:

    Article Title: Mxi1 participates in the progression of lung cancer via the microRNA-300/KLF9/GADD34 Axis.
    Article Snippet: .. CD4+ CD3+ T cells were isolated using mouse T cell isolation kit (R&D system) according to the manufacturer’s instructions. ..

    Article Title: Mxi1 participates in the progression of lung cancer via the microRNA-300/KLF9/GADD34 Axis
    Article Snippet: .. CD4 + CD3 + T cells were isolated using mouse T cell isolation kit (R&D system) according to the manufacturer’s instructions. ..

    Article Title: Hypercholesterolemic Dysregulation of Calpain in Lymphatic Endothelial Cells Interferes With Regulatory T-Cell Stability and Trafficking
    Article Snippet: .. Then the cells were labeled by using a MagCellect Mouse CD4+ T-Cell Isolation Kit (R&D Systems, Inc) and were separated by magnetic fields using negative selection. .. To obtain Tregs, CD4+ cells were labeled by using MagCellect Mouse CD4+CD25+ Regulatory T-Cell Isolation Kit (R&D Systems, Inc), and were separated by magnetic fields using positive selection.

    Article Title: Hepatocellular Carcinoma Cells Up-regulate PVRL1, Stabilizing PVR and Inhibiting the Cytotoxic T-Cell Response via TIGIT to Mediate Tumor Resistance to PD1 Inhibitors in Mice.
    Article Snippet: .. Isolation of T cells was carried out with mouse T cell isolation kit (R&D Systems) according to the manufacturer’s instruction. .. T cells were stained with 2 μM carboxyfluorescein succinimidyl ester (CSFE; Thermo Fisher) for 10 min and in vitro activated with CD3/CD28 dynabeads (Thermo Fisher) for 24 hr, and subsequently co- cultured with Hepa1-6 cells in 1:1 for 3 days.

    Article Title: Bufalin stimulates antitumor immune response by driving tumor-infiltrating macrophage toward M1 phenotype in hepatocellular carcinoma
    Article Snippet: Fresh murine spleen isolated from C57BL/6 mice was torn apart and passed through a 70 μm nylon strainer (BD Falcon) to obtain single cell suspensions. .. CD4 + and CD8 + T cells were isolated using MagCellect Mouse CD4 + and CD8 + T cell isolation kit (MAGM202 and 203, R&D systems), respectively. .. For T cell proliferation assay, CD4 + or CD8 + T cells were labeled with carboxyfluorescein succinimidyl ester (CFSE, Invitrogen) and co-cultured with bufalin-primed BMDM (1:1) or bufalin-treated TIM (1:1) in the presence or absence of 2.5 μg/mL anti-CD3 (Invitrogen) and 5 μg/mL anti-CD28 (Invitrogen) for 3 days.

    Cell Isolation:

    Article Title: Mxi1 participates in the progression of lung cancer via the microRNA-300/KLF9/GADD34 Axis.
    Article Snippet: .. CD4+ CD3+ T cells were isolated using mouse T cell isolation kit (R&D system) according to the manufacturer’s instructions. ..

    Article Title: Mxi1 participates in the progression of lung cancer via the microRNA-300/KLF9/GADD34 Axis
    Article Snippet: .. CD4 + CD3 + T cells were isolated using mouse T cell isolation kit (R&D system) according to the manufacturer’s instructions. ..

    Article Title: Hepatocellular Carcinoma Cells Up-regulate PVRL1, Stabilizing PVR and Inhibiting the Cytotoxic T-Cell Response via TIGIT to Mediate Tumor Resistance to PD1 Inhibitors in Mice.
    Article Snippet: .. Isolation of T cells was carried out with mouse T cell isolation kit (R&D Systems) according to the manufacturer’s instruction. .. T cells were stained with 2 μM carboxyfluorescein succinimidyl ester (CSFE; Thermo Fisher) for 10 min and in vitro activated with CD3/CD28 dynabeads (Thermo Fisher) for 24 hr, and subsequently co- cultured with Hepa1-6 cells in 1:1 for 3 days.

    Article Title: Bufalin stimulates antitumor immune response by driving tumor-infiltrating macrophage toward M1 phenotype in hepatocellular carcinoma
    Article Snippet: Fresh murine spleen isolated from C57BL/6 mice was torn apart and passed through a 70 μm nylon strainer (BD Falcon) to obtain single cell suspensions. .. CD4 + and CD8 + T cells were isolated using MagCellect Mouse CD4 + and CD8 + T cell isolation kit (MAGM202 and 203, R&D systems), respectively. .. For T cell proliferation assay, CD4 + or CD8 + T cells were labeled with carboxyfluorescein succinimidyl ester (CFSE, Invitrogen) and co-cultured with bufalin-primed BMDM (1:1) or bufalin-treated TIM (1:1) in the presence or absence of 2.5 μg/mL anti-CD3 (Invitrogen) and 5 μg/mL anti-CD28 (Invitrogen) for 3 days.

    Labeling:

    Article Title: Hypercholesterolemic Dysregulation of Calpain in Lymphatic Endothelial Cells Interferes With Regulatory T-Cell Stability and Trafficking
    Article Snippet: .. Then the cells were labeled by using a MagCellect Mouse CD4+ T-Cell Isolation Kit (R&D Systems, Inc) and were separated by magnetic fields using negative selection. .. To obtain Tregs, CD4+ cells were labeled by using MagCellect Mouse CD4+CD25+ Regulatory T-Cell Isolation Kit (R&D Systems, Inc), and were separated by magnetic fields using positive selection.



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    Miltenyi Biotec mouse cd4 t cell isolation kit
    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic <t>CD4</t> + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.
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    Miltenyi Biotec magnetic negative selection
    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic <t>CD4</t> + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.
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    Image Search Results


    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Journal: Immunity, Inflammation and Disease

    Article Title: TRPM2 Deficiency Attenuates Allergic Rhinitis‐Like Inflammation With Altered Ca 2+ ‐NFAT Signaling, Treg Responses, and sIgE Production

    doi: 10.1002/iid3.70490

    Figure Lengend Snippet: TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Article Snippet: Naïve CD4 + T cells were then isolated by negative selection using a commercial mouse CD4 + T Cell Isolation Kit (Cat. No. 130‐104‐454, Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

    Techniques: Activation Assay, Flow Cytometry, Fluorescence, Translocation Assay, Western Blot