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130 ‑ 092 ‑ 263  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec 130 ‑ 092 ‑ 263
    130 ‑ 092 ‑ 263, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38+microbead+kit/CD38+MicroBead+Kit%2C+human/pm39611467-58-0-3
    Average 92 stars, based on 25 article reviews
    130 ‑ 092 ‑ 263 - by Bioz Stars, 2026-10
    92/100 stars

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    other:

    Article Title: Study on the in vitro changes of human bone marrow‑related mesenchymal stem cells.
    Article Snippet: 130‐092‐263 and 130‐100‐453; Miltenyi Biotec GmbH), and these cells were successively treated with CD38‐ and CD34‐labeled magnetic beads, respectively; and CD34‐positive and CD38‐negative HSCs were collected for co‐culture operation under the action of a magnetic field by the negative and positive sorting methods, respectively.

    Magnetic Cell Separation:

    Article Title: Rapid discovery of monoclonal antibodies by microfluidics-enabled FACS of single pathogen-specific antibody-secreting cells.
    Article Snippet: .. Cells were washed once in MACS buffer, and CD38+ cells were positively selected using the CD38 MicroBead kit (Miltenyi Biotec). .. Hydrogel beads were sorted on a four-laser BD FACSAria III using a 100 μm nozzle into 96-well plates (Frame Star, 4titude) that contained 4 μl of lysis buffer (3 U μl−1 RNAseOUT RNase inhibitor and 1 mM DTT in 0.5× PBS87).

    Selection:

    Article Title: High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells.
    Article Snippet: .. Vials were thawed on the day of downstream analysis and ASCs were first pre-enriched by depletion of non-plasma cells and subsequently fully enriched by positive selection with human CD38-specific mAb-conjugated MicroBeads according to the human Plasma Cell Isolation Kit II protocol (Miltenyi Biotec). .. Isolated CD38+ ASCs (both plasmablasts and plasma cells) were resuspended in cell culture medium (RPMI-1640 containing 10% FCS, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate and 1% non-essential amino acids) supplemented with 1000 U/mL human IFN-α (Thermo Fisher Scientific), 1 μg/mL TLR 7/8-ligand (L) (R848, Miltenyi Biotec) and 30 ng/mL interleukin-21 (IL-21) (Peprotech) for sustained antibody secretion and viability purposes.

    Clinical Proteomics:

    Article Title: High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells.
    Article Snippet: .. Vials were thawed on the day of downstream analysis and ASCs were first pre-enriched by depletion of non-plasma cells and subsequently fully enriched by positive selection with human CD38-specific mAb-conjugated MicroBeads according to the human Plasma Cell Isolation Kit II protocol (Miltenyi Biotec). .. Isolated CD38+ ASCs (both plasmablasts and plasma cells) were resuspended in cell culture medium (RPMI-1640 containing 10% FCS, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate and 1% non-essential amino acids) supplemented with 1000 U/mL human IFN-α (Thermo Fisher Scientific), 1 μg/mL TLR 7/8-ligand (L) (R848, Miltenyi Biotec) and 30 ng/mL interleukin-21 (IL-21) (Peprotech) for sustained antibody secretion and viability purposes.

    Cell Isolation:

    Article Title: High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells.
    Article Snippet: .. Vials were thawed on the day of downstream analysis and ASCs were first pre-enriched by depletion of non-plasma cells and subsequently fully enriched by positive selection with human CD38-specific mAb-conjugated MicroBeads according to the human Plasma Cell Isolation Kit II protocol (Miltenyi Biotec). .. Isolated CD38+ ASCs (both plasmablasts and plasma cells) were resuspended in cell culture medium (RPMI-1640 containing 10% FCS, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate and 1% non-essential amino acids) supplemented with 1000 U/mL human IFN-α (Thermo Fisher Scientific), 1 μg/mL TLR 7/8-ligand (L) (R848, Miltenyi Biotec) and 30 ng/mL interleukin-21 (IL-21) (Peprotech) for sustained antibody secretion and viability purposes.



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