Review



cd36 nb400 144  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Novus Biologicals cd36 nb400 144
    Enhanced hepatic VLDLR and <t>CD36</t> levels in Sirt3 -deficient mice fed a HFD. mRNA abundance ( a ) and protein levels ( b ) of VLDLR in livers of WT and Sirt3 −/− mice fed either a standard chow or a HFD. CD36 ( c ), total and phospho-Nrf2 ( d ), Keap1 ( e ) and NQO1 ( f ) protein levels. g , ROS levels. h , PPARγ protein levels. Data are presented as the mean ± S.D. ( n = 6 per group). a, p < 0.05 vs. WT mice fed a standard chow. b, p < 0.05 vs. WT mice fed a HFD. c, p < 0.05 vs. Sirt3 −/− mice fed a standard chow
    Cd36 Nb400 144, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/CD36+Antibody+-+BSA+Free/pmc07488148-45-46-48
    Average 95 stars, based on 170 article reviews
    cd36 nb400 144 - by Bioz Stars, 2026-11
    95/100 stars

    Images

    1) Product Images from "SIRT3 deficiency exacerbates fatty liver by attenuating the HIF1α-LIPIN 1 pathway and increasing CD36 through Nrf2"

    Article Title: SIRT3 deficiency exacerbates fatty liver by attenuating the HIF1α-LIPIN 1 pathway and increasing CD36 through Nrf2

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-020-00640-8

    Enhanced hepatic VLDLR and CD36 levels in Sirt3 -deficient mice fed a HFD. mRNA abundance ( a ) and protein levels ( b ) of VLDLR in livers of WT and Sirt3 −/− mice fed either a standard chow or a HFD. CD36 ( c ), total and phospho-Nrf2 ( d ), Keap1 ( e ) and NQO1 ( f ) protein levels. g , ROS levels. h , PPARγ protein levels. Data are presented as the mean ± S.D. ( n = 6 per group). a, p < 0.05 vs. WT mice fed a standard chow. b, p < 0.05 vs. WT mice fed a HFD. c, p < 0.05 vs. Sirt3 −/− mice fed a standard chow
    Figure Legend Snippet: Enhanced hepatic VLDLR and CD36 levels in Sirt3 -deficient mice fed a HFD. mRNA abundance ( a ) and protein levels ( b ) of VLDLR in livers of WT and Sirt3 −/− mice fed either a standard chow or a HFD. CD36 ( c ), total and phospho-Nrf2 ( d ), Keap1 ( e ) and NQO1 ( f ) protein levels. g , ROS levels. h , PPARγ protein levels. Data are presented as the mean ± S.D. ( n = 6 per group). a, p < 0.05 vs. WT mice fed a standard chow. b, p < 0.05 vs. WT mice fed a HFD. c, p < 0.05 vs. Sirt3 −/− mice fed a standard chow

    Techniques Used:

    The increase in CD36 levels caused by lipids in Sirt3 -deficient hepatocytes is mediated by Nrf2. VLDLR mRNA abundance ( a ) and protein levels of VLDLR and NQO1, an Nrf-2-target gene, ( b ) were assessed in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) and exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. c , fatty acid uptake in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) and exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h was measured by the uptake of BODIPY-C16. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. mRNA abundance ( d ) and protein levels of VLDLR ( e ) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA and incubated in the presence or absence 0.3 mM palmitate (Pal) for 24 h. Protein levels of CD36 ( f ), NQO1 ( g ) and PPARγ ( h ) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA and incubated in the presence or absence 0.3 mM palmitate (Pal) or the Nrf2 inhibitor ML385 (10 μM) for 24 h. a, p < 0.05 vs. CT siRNA cells. b, p < 0.05 vs. CT siRNA cells incubated with palmitate. c, p < 0.05 vs. SIRT3 siRNA cells. d, p < 0.05 vs. CT siRNA cells incubated with palmitate and ML385
    Figure Legend Snippet: The increase in CD36 levels caused by lipids in Sirt3 -deficient hepatocytes is mediated by Nrf2. VLDLR mRNA abundance ( a ) and protein levels of VLDLR and NQO1, an Nrf-2-target gene, ( b ) were assessed in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) and exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. c , fatty acid uptake in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) and exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h was measured by the uptake of BODIPY-C16. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. mRNA abundance ( d ) and protein levels of VLDLR ( e ) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA and incubated in the presence or absence 0.3 mM palmitate (Pal) for 24 h. Protein levels of CD36 ( f ), NQO1 ( g ) and PPARγ ( h ) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA and incubated in the presence or absence 0.3 mM palmitate (Pal) or the Nrf2 inhibitor ML385 (10 μM) for 24 h. a, p < 0.05 vs. CT siRNA cells. b, p < 0.05 vs. CT siRNA cells incubated with palmitate. c, p < 0.05 vs. SIRT3 siRNA cells. d, p < 0.05 vs. CT siRNA cells incubated with palmitate and ML385

    Techniques Used: Incubation, Transfection, Control

    Potential new mechanisms by which Sirt3 deficiency promotes hepatic steatosis in mice fed a HFD. Exposure to a HFD reduces Sirt3 and contributes to triglyceride accumulation in the liver. However, hepatic lipid accumulation is attenuated by the activation of an adaptive mechanism involving an increase in nuclear HIF-1α and Lipin 1. Longer exposures to a HFD exacerbates Sirt3 decrease leading to higher uptake of lipids through an Nrf-2 mediated increase in CD36 and VLDLR. This results in a higher increase in fatty acid accumulation, which in turn reduces succinate levels, ultimately suppressing the adaptive increase in HIF-1α and Lipin 1. FAO: Fatty acid oxidation
    Figure Legend Snippet: Potential new mechanisms by which Sirt3 deficiency promotes hepatic steatosis in mice fed a HFD. Exposure to a HFD reduces Sirt3 and contributes to triglyceride accumulation in the liver. However, hepatic lipid accumulation is attenuated by the activation of an adaptive mechanism involving an increase in nuclear HIF-1α and Lipin 1. Longer exposures to a HFD exacerbates Sirt3 decrease leading to higher uptake of lipids through an Nrf-2 mediated increase in CD36 and VLDLR. This results in a higher increase in fatty acid accumulation, which in turn reduces succinate levels, ultimately suppressing the adaptive increase in HIF-1α and Lipin 1. FAO: Fatty acid oxidation

    Techniques Used: Activation Assay

    Related Articles

    Milk:

    Article Title: Elevated diurnal CD36 expression disrupts the bile acid synthesis rhythm leading to cholestatic liver injury and inflammation via the HMGCR/CYP7A1 axis
    Article Snippet: was performed via an enhanced chemiluminescence kit (Amersham Bioscience, US), and quantitative analysis was conducted via ImageJ software (v1.53k). The primary antibodies utilized included anti-CD36 (Novus, Cat# NB400-144, RRID:AB_10003498), anti-HMGCR (ABclonal, Cat# A19063, RRID:AB_2862556), anti-CYP7A1 (Affinity Biosciences, Cat# DF2612, RRID:AB_2839818), anti-NR0B2 (Affinity Biosciences, Cat#

    Western Blot:

    Article Title: Elevated diurnal CD36 expression disrupts the bile acid synthesis rhythm leading to cholestatic liver injury and inflammation via the HMGCR/CYP7A1 axis
    Article Snippet: was performed via an enhanced chemiluminescence kit (Amersham Bioscience, US), and quantitative analysis was conducted via ImageJ software (v1.53k). The primary antibodies utilized included anti-CD36 (Novus, Cat# NB400-144, RRID:AB_10003498), anti-HMGCR (ABclonal, Cat# A19063, RRID:AB_2862556), anti-CYP7A1 (Affinity Biosciences, Cat# DF2612, RRID:AB_2839818), anti-NR0B2 (Affinity Biosciences, Cat#



    Similar Products

    cd36 antibody  (NSJ Bioreagents)
    94
    NSJ Bioreagents cd36 antibody
    Cd36 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/CD36+Antibody/custom%40v3002%4010%2E13052%2Fijts2246-8765%2E2026%2E006
    Average 94 stars, based on 1 article reviews
    cd36 antibody - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    gene exp cd36 mm00432403 m1  (Thermo Fisher)
    99
    Thermo Fisher gene exp cd36 mm00432403 m1
    Impact of high fat feeding and voluntary exercise on left ventricle morphology in male mice. (A) Representative images of Picrosirius red staining for measurement of interstitial and perivascular fibrosis with 20× magnification. (B) Representative images for Hematoxylin and Eosin for measurement of cardiomyocyte width and area with 40× magnification. (C) Representative images of Oil red O staining for measurement of interstitial lipid deposition with 40× magnification. Quantitative analysis of percent area of (D) interstitial and (E) perivascular fibrosis expressed as fold change from sedentary chow group. Quantitative analysis of (F) cardiomyocyte width and (G) cardiomyocyte area. (H) Quantitative analysis of percent area of lipid deposition expressed as fold change from sedentary chow group left ventricle mRNA expression of fatty acid transporters (I) FABP3, (J) <t>CD36,</t> and hypertrophy markers (K) β-MHC and (L) ANP in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (D–H) n : 8–12 per group. (I–L) n : 8–9 per group. * p < 0.05, **** p < 0.0001. ANP = atrial natriuretic peptide; β-MHC = beta myosin heavy chain; CD36 = platelet glycoprotein 4; FABP3 = fatty acid-binding protein 3; HFD = high fat diet; Myh7 = moysin heavy chain 7; VET = voluntary exercise training.
    Gene Exp Cd36 Mm00432403 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/Gene+Exp%2E+Cd36%2C+Mm00432403_m1/pmc12811436-61-18--1
    Average 99 stars, based on 1 article reviews
    gene exp cd36 mm00432403 m1 - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    rabbit anti cd36  (Proteintech)
    96
    Proteintech rabbit anti cd36
    Impact of high fat feeding and voluntary exercise on left ventricle morphology in male mice. (A) Representative images of Picrosirius red staining for measurement of interstitial and perivascular fibrosis with 20× magnification. (B) Representative images for Hematoxylin and Eosin for measurement of cardiomyocyte width and area with 40× magnification. (C) Representative images of Oil red O staining for measurement of interstitial lipid deposition with 40× magnification. Quantitative analysis of percent area of (D) interstitial and (E) perivascular fibrosis expressed as fold change from sedentary chow group. Quantitative analysis of (F) cardiomyocyte width and (G) cardiomyocyte area. (H) Quantitative analysis of percent area of lipid deposition expressed as fold change from sedentary chow group left ventricle mRNA expression of fatty acid transporters (I) FABP3, (J) <t>CD36,</t> and hypertrophy markers (K) β-MHC and (L) ANP in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (D–H) n : 8–12 per group. (I–L) n : 8–9 per group. * p < 0.05, **** p < 0.0001. ANP = atrial natriuretic peptide; β-MHC = beta myosin heavy chain; CD36 = platelet glycoprotein 4; FABP3 = fatty acid-binding protein 3; HFD = high fat diet; Myh7 = moysin heavy chain 7; VET = voluntary exercise training.
    Rabbit Anti Cd36, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/CD36+Antibody/10__1038_slash_s41467___026___78907___w-480-158-160
    Average 96 stars, based on 1 article reviews
    rabbit anti cd36 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    anti cd36  (Novus Biologicals)
    93
    Novus Biologicals anti cd36
    Impact of high fat feeding and voluntary exercise on left ventricle morphology in male mice. (A) Representative images of Picrosirius red staining for measurement of interstitial and perivascular fibrosis with 20× magnification. (B) Representative images for Hematoxylin and Eosin for measurement of cardiomyocyte width and area with 40× magnification. (C) Representative images of Oil red O staining for measurement of interstitial lipid deposition with 40× magnification. Quantitative analysis of percent area of (D) interstitial and (E) perivascular fibrosis expressed as fold change from sedentary chow group. Quantitative analysis of (F) cardiomyocyte width and (G) cardiomyocyte area. (H) Quantitative analysis of percent area of lipid deposition expressed as fold change from sedentary chow group left ventricle mRNA expression of fatty acid transporters (I) FABP3, (J) <t>CD36,</t> and hypertrophy markers (K) β-MHC and (L) ANP in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (D–H) n : 8–12 per group. (I–L) n : 8–9 per group. * p < 0.05, **** p < 0.0001. ANP = atrial natriuretic peptide; β-MHC = beta myosin heavy chain; CD36 = platelet glycoprotein 4; FABP3 = fatty acid-binding protein 3; HFD = high fat diet; Myh7 = moysin heavy chain 7; VET = voluntary exercise training.
    Anti Cd36, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/CD36+Antibody+-+BSA+Free/pm42883793-259-35-37
    Average 93 stars, based on 1 article reviews
    anti cd36 - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    cd36  (Proteintech)
    96
    Proteintech cd36
    C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , <t>CD36</t> , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001
    Cd36, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/CD36+Antibody/pmc13675466-329-19-21
    Average 96 stars, based on 1 article reviews
    cd36 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    anti cd36 antibody  (Proteintech)
    96
    Proteintech anti cd36 antibody
    C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , <t>CD36</t> , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001
    Anti Cd36 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/CD36+Antibody/pm42861157-135-2-4
    Average 96 stars, based on 1 article reviews
    anti cd36 antibody - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    cd36 inhibitor sso  (MedChemExpress)
    95
    MedChemExpress cd36 inhibitor sso
    C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , <t>CD36</t> , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001
    Cd36 Inhibitor Sso, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/Sulfosuccinimidyl+oleate+sodium/pm42861157-168-12-19
    Average 95 stars, based on 1 article reviews
    cd36 inhibitor sso - by Bioz Stars, 2026-11
    95/100 stars
    		  Buy from Supplier
    cd36 inhibitor sulfosuccinimidyl oleate  (MedChemExpress)
    94
    MedChemExpress cd36 inhibitor sulfosuccinimidyl oleate
    C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , <t>CD36</t> , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001
    Cd36 Inhibitor Sulfosuccinimidyl Oleate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/Medronic+acid/pm42861157-92-13-18
    Average 94 stars, based on 1 article reviews
    cd36 inhibitor sulfosuccinimidyl oleate - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    cd36  (Bio-Rad)
    93
    Bio-Rad cd36
    C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , <t>CD36</t> , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001
    Cd36, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36/Rat+anti+Mouse+CD36/pm42849443-1194-49-51
    Average 93 stars, based on 1 article reviews
    cd36 - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Impact of high fat feeding and voluntary exercise on left ventricle morphology in male mice. (A) Representative images of Picrosirius red staining for measurement of interstitial and perivascular fibrosis with 20× magnification. (B) Representative images for Hematoxylin and Eosin for measurement of cardiomyocyte width and area with 40× magnification. (C) Representative images of Oil red O staining for measurement of interstitial lipid deposition with 40× magnification. Quantitative analysis of percent area of (D) interstitial and (E) perivascular fibrosis expressed as fold change from sedentary chow group. Quantitative analysis of (F) cardiomyocyte width and (G) cardiomyocyte area. (H) Quantitative analysis of percent area of lipid deposition expressed as fold change from sedentary chow group left ventricle mRNA expression of fatty acid transporters (I) FABP3, (J) CD36, and hypertrophy markers (K) β-MHC and (L) ANP in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (D–H) n : 8–12 per group. (I–L) n : 8–9 per group. * p < 0.05, **** p < 0.0001. ANP = atrial natriuretic peptide; β-MHC = beta myosin heavy chain; CD36 = platelet glycoprotein 4; FABP3 = fatty acid-binding protein 3; HFD = high fat diet; Myh7 = moysin heavy chain 7; VET = voluntary exercise training.

    Journal: Journal of Sport and Health Science

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

    doi: 10.1016/j.jshs.2025.101095

    Figure Lengend Snippet: Impact of high fat feeding and voluntary exercise on left ventricle morphology in male mice. (A) Representative images of Picrosirius red staining for measurement of interstitial and perivascular fibrosis with 20× magnification. (B) Representative images for Hematoxylin and Eosin for measurement of cardiomyocyte width and area with 40× magnification. (C) Representative images of Oil red O staining for measurement of interstitial lipid deposition with 40× magnification. Quantitative analysis of percent area of (D) interstitial and (E) perivascular fibrosis expressed as fold change from sedentary chow group. Quantitative analysis of (F) cardiomyocyte width and (G) cardiomyocyte area. (H) Quantitative analysis of percent area of lipid deposition expressed as fold change from sedentary chow group left ventricle mRNA expression of fatty acid transporters (I) FABP3, (J) CD36, and hypertrophy markers (K) β-MHC and (L) ANP in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (D–H) n : 8–12 per group. (I–L) n : 8–9 per group. * p < 0.05, **** p < 0.0001. ANP = atrial natriuretic peptide; β-MHC = beta myosin heavy chain; CD36 = platelet glycoprotein 4; FABP3 = fatty acid-binding protein 3; HFD = high fat diet; Myh7 = moysin heavy chain 7; VET = voluntary exercise training.

    Article Snippet: The following TaqMan assay gene transcripts were used: fatty acid-binding protein 3 (FABP3, Mm02342495_m1), platelet glycoprotein 4 (CD36, Mm00432403_m1), beta myosin heavy chain (β-MHC, Mm00600555_m1), atrial natriuretic peptide (ANP, Mm01255747_g1), OPA1 (Mm01349707_g1), DRP1 (Mm01342903_m1), robosomal18S (18S, Mm03928990_g1).

    Techniques: Staining, Expressing, Binding Assay

    C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , CD36 , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Molecular Biomedicine

    Article Title: Curcumin alleviates metabolic dysfunction-associated steatotic liver disease via gut microbiota-dependent epigenetic alterations

    doi: 10.1186/s43556-026-00606-1

    Figure Lengend Snippet: C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , CD36 , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: The primary antibodies used were as follows: AHR (67785–1-Ig, Proteintech, China), CEBPB (23431–1-AP, Proteintech, China), ACC1 (21923–1-AP, Proteintech, China), CD36 (18836–1-AP, Proteintech, China), SCD1 (28678–1-AP, Proteintech, China), phosphorylated PI3K (p-PI3K, 138364, Abcam, USA), PI3K (4257, CST, USA), phosphorylated AKT (p-AKT, 66444–1-Ig, Proteintech, China), AKT (10176–2-AP, Proteintech, China), and β-actin (66009–1-Ig, Proteintech, China).

    Techniques: Fluorescence, Staining, Analysis, Control, Transfection, Negative Control, Western Blot