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cd34 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd34 microbeads
    Cd34 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1655 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+microbead+kit/CD34+MicroBead+Kit%2C+human/pm41991719-25-7-9
    Average 97 stars, based on 1655 article reviews
    cd34 microbeads - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).

    Gradient Centrifugation:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).

    Purification:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Modular Microfluidic Device for Intensified CAR T Cell Expansion
    Article Snippet: .. CAR T cells were purified using a CD34 MicroBead Kit and LS Columns (Miltenyi Biotec Ltd., UK) [37]. .. Purified CAR T cells were co-cultured at a 1:1 (CAR-T cell: target cell) ratio with GFP + , CD19 + NALM6 target cells in poly-L-lysine-coated 96-well plates (Sigma Aldrich Merck, UK; Sarstedt AG & Co. KG, Germany).

    Selection:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Magnetic Cell Separation:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Immunostaining:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Cell Isolation:

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).



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    B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed <t>progenitor</t> <t>cells</t> (A) Flow cytometric analysis of <t>CD34,</t> c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted <t>CD34</t> + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .
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    Image Search Results


    B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed progenitor cells (A) Flow cytometric analysis of CD34, c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .

    Journal: Cell Reports Medicine

    Article Title: ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development

    doi: 10.1016/j.xcrm.2026.102879

    Figure Lengend Snippet: B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed progenitor cells (A) Flow cytometric analysis of CD34, c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .

    Article Snippet: Leukocytes from UCB and mPB were first purified using the Erythrocyte Sedimentation Kit II (Miltenyi), followed by CD34 + cell enrichment with the CD34 MicroBead Kit UltraPure (Miltenyi) per manufacturer’s instructions.

    Techniques: Expressing