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human cd34 microbead kit  (Miltenyi Biotec)


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    Miltenyi Biotec human cd34 microbead kit
    Table of Materials
    Human Cd34 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1668 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34++fraction/CD34+MicroBead+Kit%2C+human/pmc07046082-24-0-5
    Average 97 stars, based on 1668 article reviews
    human cd34 microbead kit - by Bioz Stars, 2026-11
    97/100 stars

    Images

    1) Product Images from "Pan-Myeloid Differentiation of Human Cord Blood Derived CD34 + Hematopoietic Stem and Progenitor Cells"

    Article Title: Pan-Myeloid Differentiation of Human Cord Blood Derived CD34 + Hematopoietic Stem and Progenitor Cells

    Journal: Journal of visualized experiments : JoVE

    doi: 10.3791/59836

    Table of Materials
    Figure Legend Snippet: Table of Materials

    Techniques Used: Sterility, Staining, Modification, Concentration Assay, Fractionation, Recombinant, Flow Cytometry, Light Microscopy

    (A) Cells were gated on forward and side scatter to select a single cell population. Dead and lineage positive cells were eliminated by staining with 7-AAD and antibodies to CD3, CD7, CD10, CD11b, CD19, and CD235a (all FITC stained). The Lin−/live cells were analyzed with antibodies to CD34 (APC-Cy7), CD38 (PE), CD123 (APC), and CD45RA (PE-Cy7). The progenitors were distinguished from the CD34+/CD38+ cells. CMPs are CD123lo/CD45RA−, GMPs are CD123lo/CD45RA+ and MEPs are CD123−/CD45RA−. Representative scatter plots from an experiment are shown. (B) Percentages of total HSPCs (total CD34+ cells), CMPs, GMPs, and MEPs in cord blood are represented. Data presented are averages with standard error from at least 3 experiments.
    Figure Legend Snippet: (A) Cells were gated on forward and side scatter to select a single cell population. Dead and lineage positive cells were eliminated by staining with 7-AAD and antibodies to CD3, CD7, CD10, CD11b, CD19, and CD235a (all FITC stained). The Lin−/live cells were analyzed with antibodies to CD34 (APC-Cy7), CD38 (PE), CD123 (APC), and CD45RA (PE-Cy7). The progenitors were distinguished from the CD34+/CD38+ cells. CMPs are CD123lo/CD45RA−, GMPs are CD123lo/CD45RA+ and MEPs are CD123−/CD45RA−. Representative scatter plots from an experiment are shown. (B) Percentages of total HSPCs (total CD34+ cells), CMPs, GMPs, and MEPs in cord blood are represented. Data presented are averages with standard error from at least 3 experiments.

    Techniques Used: Staining

    Single cells were gated based on forward and side scatter. CD34− cells were selected by staining with antibody to CD34 (APC-Cy7). Myeloid lineages in the CD34− population were analyzed with antibodies to CD14 (PE), CD66b (PE-Cy7), CD41 (PerCP-Cy5.5), and CD235a (APC) on day 1 (A) and day 21 (B). Monocytes are CD14+/CD66b−, granulocytes are CD14−/CD66b+, megakaryocytes are CD41+/CD235a− and erythroid cells are CD41−/CD235a+. Representative scatter plots from an experiment are shown. Fractions of monocytes, granulocytes, megakaryocytes, and erythroid cells in the CD34− population on day 1 and day 21 are represented (C). Data presented are averages with standard error from at least 3 experiments. This figure has been modified from Bapat et al.11.
    Figure Legend Snippet: Single cells were gated based on forward and side scatter. CD34− cells were selected by staining with antibody to CD34 (APC-Cy7). Myeloid lineages in the CD34− population were analyzed with antibodies to CD14 (PE), CD66b (PE-Cy7), CD41 (PerCP-Cy5.5), and CD235a (APC) on day 1 (A) and day 21 (B). Monocytes are CD14+/CD66b−, granulocytes are CD14−/CD66b+, megakaryocytes are CD41+/CD235a− and erythroid cells are CD41−/CD235a+. Representative scatter plots from an experiment are shown. Fractions of monocytes, granulocytes, megakaryocytes, and erythroid cells in the CD34− population on day 1 and day 21 are represented (C). Data presented are averages with standard error from at least 3 experiments. This figure has been modified from Bapat et al.11.

    Techniques Used: Staining, Modification

    CD34+ HSPCs (A) and cells from myeloid cultures at day 21 (B) were stained with the Wright-Giemsa stain. Cells corresponding to granulocytes (black arrows), monocytes (blue arrows) and erythroid cells (red arrows) are shown. Scale bars represent 10 μM. This figure has been modified from Bapat et al.11.
    Figure Legend Snippet: CD34+ HSPCs (A) and cells from myeloid cultures at day 21 (B) were stained with the Wright-Giemsa stain. Cells corresponding to granulocytes (black arrows), monocytes (blue arrows) and erythroid cells (red arrows) are shown. Scale bars represent 10 μM. This figure has been modified from Bapat et al.11.

    Techniques Used: Staining, Giemsa Stain, Modification

    Related Articles

    Isolation:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).

    Gradient Centrifugation:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).

    Purification:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Article Title: Modular Microfluidic Device for Intensified CAR T Cell Expansion
    Article Snippet: .. CAR T cells were purified using a CD34 MicroBead Kit and LS Columns (Miltenyi Biotec Ltd., UK) [37]. .. Purified CAR T cells were co-cultured at a 1:1 (CAR-T cell: target cell) ratio with GFP + , CD19 + NALM6 target cells in poly-L-lysine-coated 96-well plates (Sigma Aldrich Merck, UK; Sarstedt AG & Co. KG, Germany).

    Selection:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Magnetic Cell Separation:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Immunostaining:

    Article Title: Case Report: Recurrence of recipient-derived erythropoiesis in hematopoietic stem cell transplant for sickle-cell anemia: multi-lineage chimerism analysis as a tool to distinguish atypical pure red cell aplasia from poor erythroid engraftment
    Article Snippet: .. Bone marrow mononuclear cells were first isolated by density gradient centrifugation over Lymphocytes Separation Medium (MSL , Eurobio Scientific; density 1.078 g/mL), and CD34+ multipotent hematopoietic stem/progenitor cells (HSPCs) were subsequently purified from the mononuclear fraction by immunomagnetic selection with MACS columns (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Purified CD34+ HSPCs were plated at a concentration of 250 cells/mL in a methylcellulose-based medium (GFH4435, Stem Cell Technologies) under conditions supporting the growth and differentiation of committed erythroid (Burst-Forming Unit-Erythrocytes, BFU-E) and granulo-monocytic (Colony-Forming Unit-Granulocytes Macrophages, CFU-GM) progenitors and incubated at 37 °C in a humidified atmosphere with 5% CO2.

    Cell Isolation:

    Article Title: Engineering IL-4 resistant proinflammatory human myeloid cells for cancer immunotherapy.
    Article Snippet: Written informed consent was obtained from each donor prior to specimen collection, in keeping with the Declaration of Helsinki and under the auspices of a research protocol approved by the Johns Hopkins University Institutional Review Board (protocol IRB00127577). .. Mononuclear cells were isolated by density gradient centrifugation (Ficoll-Paque; GE Healthcare Life Sciences, Piscataway, NJ, USA), followed by CD34+ cell isolation using a CD34 MicroBead Kit (Miltenyi Biotech, Gaithersburg, MD, USA) in the absence of serum. .. CD34+ cells were cultured in serum-free Expansion Media, consisting of StemSpan SFEM II (StemCell Technologies, Vancouver, Canada) supplemented with human stem cell factor (SCF, 100 ng mL 1; Peprotech, Cranbury, NJ, USA), FLT3 ligand (FL, 100 ng mL 1; Peprotech), thrombopoietin (TPO, 100 ng mL 1; Peprotech), interleukin-6 (IL-6, 100 ng mL 1; Peprotech), SR-1 (750 nM; Cellagen Technology, San Diego, CA, USA), UM171 (35 nM; ExcellThera, Inc., Montreal, Canada), and antibiotic-antimycotic (AA).



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    Image Search Results


    FDA/EMA approved gene therapy product for rare monogenic diseases

    Journal: Clinical and Experimental Pediatrics

    Article Title: Development of orphan drugs for rare diseases

    doi: 10.3345/cep.2023.00535

    Figure Lengend Snippet: FDA/EMA approved gene therapy product for rare monogenic diseases

    Article Snippet: For instance, an autologous CD34+-enriched cell fraction, Strimvelis (GlaxoSmithKline plk, London, UK), which contains CD34+ cells transduced with a retroviral vector that encodes the human ADA cDNA sequence for ADA deficiency (introduced in 2016), and voretigene neparvovec-rzyl (Luxturna, Spark Therapeutics, Philadelphia, USA), for retinal dystrophy caused by mutations in the RPE65 gene, were approved by the FDA in 2017 at a cost of $850,000 per eye [ ].

    Techniques: Plasmid Preparation, In Vivo, Ex Vivo, Mutagenesis, Modification, Variant Assay