cd34- fraction (STEMCELL Technologies Inc)
90
Structured Review
STEMCELL Technologies Inc
cd34- fraction
Cd34 Fraction, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd34++fraction/human+cd34++cord+blood+cells+hla+a24+02/pmc05563168-72-14-10
Average 90 stars, based on 1 article reviews
Cd34 Fraction, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd34++fraction/human+cd34++cord+blood+cells+hla+a24+02/pmc05563168-72-14-10
Average 90 stars, based on 1 article reviews
cd34- fraction - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Adaptive from Innate: Human IFN-γ + CD4 + T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults"
Article Title: Adaptive from Innate: Human IFN-γ + CD4 + T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults
Journal: The Journal of Immunology Author Choice
doi: 10.4049/jimmunol.1700551
Figure Legend Snippet: CXCL8-producing T cells decline with age in humans and in vivo in humanized mice and are enriched in RTEs. ( A ) CXCL8 production was determined in naive CD4 + T cells (4 h PI + BFA) obtained from 45 individuals (aged between 3 mo and 57 y). ( B ) Irradiated NSG mice were reconstituted with human CD34 + cells (CB derived). The graph shows reconstitution of T cells over time in individual mice (gray lines), with a reciprocal decline in T cell production of CXCL8 (black lines). ( C ) FACS plots show an example of this reciprocal change in one reconstituted NSG mouse; percentage of cells expressing CD3 within human CD45 + cells (upper panels) and percentage of cells expressing CXCL8 among CD3 T cells (lower panels; PI + BFA or BFA alone for 4 h). CD4 + T cells from adults ( D and F ) or children aged 1–12 y ( E ) were activated with PI (4 h), and cytokine production was determined by intracellular staining. Individual (cytokine + ) subsets were then sorted, and TREC content was determined by qPCR. Results are shown as TREC levels per million naive CD4 + cells or TREC levels per million total CD4 + cells in (F), because very few naive CD4 + cells express IFN-γ. Trend lines depict the differences in TREC levels among the four sorted populations in three patients. ( G ) CXCL8 production was determined following activation in vitro with PI (4 h, in the presence of BFA) in primary T-ALL samples ( n = 12); later stages (T-III/T-IV) are represented by gray diamonds.
Techniques Used: In Vivo, Irradiation, Derivative Assay, Expressing, Staining, Activation Assay, In Vitro
Figure Legend Snippet: CXCL8 production is imprinted in the thymus. CXCL8 production was determined following activation in vitro with PI (4 h, in the presence of BFA) in whole thymocytes versus peripheral blood from humanized mice (37 wk post–hematopoietic stem cell inoculation, n = 7) ( A ), sorted human thymocyte subsets ( n = 15, CD4 − CD8 − DN, CD4 + CD8 + double positive, CD4 − CD8 + SP CD8, CD4 + CD8 − SP CD4 + ) ( B ), and enriched T-lineage–committed DN thymocytes (defined as CD4 − CD8 − CD34 + CD7 + CD5 + CD1a + , n = 6) ( C ). Example and cumulative data are shown. Stimulation: BFA only in dark gray and PI+BFA in light gray. ( D ) Comparison of CXCL8 production between paired human SP CD4 + thymocytes and naive (CD31 + CD45RA + ) peripheral CD4 + T cells ( n = 10). * p < 0.05, ** p < 0.001.
Techniques Used: Activation Assay, In Vitro, Comparison
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