cd34 multisort kit (Miltenyi Biotec)
Structured Review
Cd34 Multisort Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd34++fraction/CD34+MultiSort+Kit%2C+human/pmc02200861-89-42-44
Average 93 stars, based on 95 article reviews
Images
Related Articles
Isolation:Article Title: Ginsenoside Rg1 promotes fetal hemoglobin production in vitro: A potential therapeutic avenue for β-thalassemia. Article Snippet: This mixture was then subjected to a density gradient centrifugation process using LymphoprepTM (STEMCELL Technologies, Canada), Centrifuge the mixture at 800×g for 30 min at room temperature to separate the mononuclear cell fraction. .. The isolation and purification of CD34+ cells from the mononuclear cell fraction were meticulously executed using the Article Title: Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci Article Snippet: .. After isolation by HISTOPAQUE (Sigma‐Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34 + cells were enriched from Mononuclear cells through positive immunomagnetic selection ( Article Title: Total ginsenosides enhance γ-globin expression and fetal hemoglobin production in β-thalassemia models Article Snippet: .. The mononuclear cell fraction was isolated using LymphoprepTM (STEMCELL Technologies, Canada), and subsequent purification of CD34 + cells was performed using the Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells. Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Article Title: Deciphering the Functional Long Non-Coding RNAs Derived from MicroRNA Loci. Article Snippet: .. After isolation by HISTOPAQUE (Sigma-Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34+ cells were enriched from Mononuclear cells through positive immunomagnetic selection ( Article Title: Galectin-1: An important regulator in myeloid differentiation and acute myeloid leukemia as well as a promising prognostic indicator and therapeutic target. Article Snippet: Acute myeloid leukemia (AML) is an aggressive and heterogeneous hematological malignancy with a low survival probability and limited therapeutic options.. Although galectin-1 (LGALS1) has been implicated in tumor cell survival and immune evasion in solid tumor, its role in AML is still unclear.. In this study, we found that LGALS1 presents prominent upregulation in AML patients at both mRNA and protein levels compared with the control samples. Purification:Article Title: Ginsenoside Rg1 promotes fetal hemoglobin production in vitro: A potential therapeutic avenue for β-thalassemia. Article Snippet: This mixture was then subjected to a density gradient centrifugation process using LymphoprepTM (STEMCELL Technologies, Canada), Centrifuge the mixture at 800×g for 30 min at room temperature to separate the mononuclear cell fraction. .. The isolation and purification of CD34+ cells from the mononuclear cell fraction were meticulously executed using the Article Title: Total ginsenosides enhance γ-globin expression and fetal hemoglobin production in β-thalassemia models Article Snippet: .. The mononuclear cell fraction was isolated using LymphoprepTM (STEMCELL Technologies, Canada), and subsequent purification of CD34 + cells was performed using the Gradient Centrifugation:Article Title: Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci Article Snippet: .. After isolation by HISTOPAQUE (Sigma‐Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34 + cells were enriched from Mononuclear cells through positive immunomagnetic selection ( Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells. Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Article Title: Deciphering the Functional Long Non-Coding RNAs Derived from MicroRNA Loci. Article Snippet: .. After isolation by HISTOPAQUE (Sigma-Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34+ cells were enriched from Mononuclear cells through positive immunomagnetic selection ( Selection:Article Title: Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci Article Snippet: .. After isolation by HISTOPAQUE (Sigma‐Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34 + cells were enriched from Mononuclear cells through positive immunomagnetic selection ( Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells. Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Article Title: Deciphering the Functional Long Non-Coding RNAs Derived from MicroRNA Loci. Article Snippet: .. After isolation by HISTOPAQUE (Sigma-Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34+ cells were enriched from Mononuclear cells through positive immunomagnetic selection ( Article Title: Galectin-1: An important regulator in myeloid differentiation and acute myeloid leukemia as well as a promising prognostic indicator and therapeutic target. Article Snippet: Acute myeloid leukemia (AML) is an aggressive and heterogeneous hematological malignancy with a low survival probability and limited therapeutic options.. Although galectin-1 (LGALS1) has been implicated in tumor cell survival and immune evasion in solid tumor, its role in AML is still unclear.. In this study, we found that LGALS1 presents prominent upregulation in AML patients at both mRNA and protein levels compared with the control samples. Derivative Assay:Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells. Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Article Title: Galectin-1: An important regulator in myeloid differentiation and acute myeloid leukemia as well as a promising prognostic indicator and therapeutic target. Article Snippet: Acute myeloid leukemia (AML) is an aggressive and heterogeneous hematological malignancy with a low survival probability and limited therapeutic options.. Although galectin-1 (LGALS1) has been implicated in tumor cell survival and immune evasion in solid tumor, its role in AML is still unclear.. In this study, we found that LGALS1 presents prominent upregulation in AML patients at both mRNA and protein levels compared with the control samples. Magnetic Cell Separation:Article Title: Immune Surveillance of Acute Myeloid Leukemia Is Mediated by HLA-Presented Antigens on Leukemia Progenitor Cells Article Snippet: For FACS, PBMCs were stained with APC anti-human CD34 (BD Biosciences, catalog no. 340441, RRID: AB_400514, clone 8G12), PE/Cy7 antihuman CD38, and PerCP/Cy5.5 anti-human CD3 (BioLegend, catalog no. 300328, RRID: AB_1575008, clone HIT3a), CD19 (BioLegend, catalog no. 302229, RRID: AB_2275547, clone HIB19), CD20 (BioLegend, catalog no. 302325, RRID: AB_893285, clone 2H7), and CD56 (BioLegend, catalog no. 318321, RRID: AB_893391, clone HCD56) mAbs and sorted on a FACSAria II or FACSAria III (BD Biosciences). .. MACS was performed with the |
