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cd19 microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec cd19 microbeads
    Cd19 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd3+selection+kit/MACSprep+CD3+and+CD19+Selection+Kit%2C+human/pmc13114487-50-16-18
    Average 94 stars, based on 2 article reviews
    cd19 microbeads - by Bioz Stars, 2026-09
    94/100 stars

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    other:

    Article Title: Enhancement of CD117-targeted bispecific T-cell engagement by CD33-targeted bispecific T-cell co-stimulation in acute myeloid leukemia
    Article Snippet: In selected cases, a CD3+/CD19+ double depletion was performed using human CD3 and CD19 MicroBeads (Miltenyi Biotec, cat# 130-136-718), following the manufacturer’s protocol.

    Article Title: Enhancement of CD117-Targeted Bispecific T-cell Engagement by CD33-Targeted Bispecific T-cell Costimulation in Acute Myeloid Leukemia
    Article Snippet: In selected cases, a CD3 + /CD19 + double depletion was performed using human CD3 and CD19 MicroBeads (Miltenyi Biotec, cat. #130-136-718), following the manufacturer’s protocol.

    Control:

    Article Title: Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti-tumor responses and phagocytic uptake of tumor-associated antigen for T cell cross-presentation.
    Article Snippet: Parallel plates were prepared for supernatant collection at 120 h. Cytokine secretion was measured using the Ella automated immunoassay platform (Biotechne, Minneapolis, MN). .. SCC152 E7-expressing TMEM30A KO cells were trypsinized, counted, and plated for 72 h at 37 C. CER-1236 or control T cells were added to each well in the presence or absence of the TLR2 inhibitor C29 and co-cultured for 24 h at 37 C. At the end of co-culture, cells were resuspended in autoMACS Rinsing Solution (Miltenyi Biotec) and CD3+ cells were isolated using a CD3 Positive Selection Kit (Miltenyi Biotec). .. CER-1236 or control T cells were counted, then cocultured with CTV-labeled E7 TCR T cells at a 1:1 ratio in the presence or absence of Ultra-LEAF Purified anti-human HLA-A,B,C (BioLegend) for 96 h. At the end of the co-culture, cells were harvested, washed, and stained using CD4 (BioLegend), CD8 (BioLegend), LIVE/DEAD Aqua (Thermo Fisher Scientific), HLADR (BioLegend), and anti-mouse TCRb (binds to E7 TCR; BioLegend).

    Article Title: Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti- tumor responses and phagocytic uptake of tumor- associated antigen for T cell cross-presentation
    Article Snippet: Parallel plates were prepared for supernatant collection at 120 h. Cytokine secretion was measured using the Ella automated immunoassay platform (Biotechne, Minneapolis, MN). .. SCC152 E7-expressing TMEM30A KO cells were trypsinized, counted, and plated for 72 h at 37 C. CER-1236 or control T cells were added to each well in the presence or absence of the TLR2 inhibitor C29 and co-cultured for 24 h at 37 C. At the end of co-culture, cells were resuspended in autoMACS Rinsing Solution (Miltenyi Biotec) and CD3+ cells were isolated using a CD3 Positive Selection Kit (Miltenyi Biotec). .. CER-1236 or control T cells were counted, then cocultured with CTV-labeled E7 TCR T cells at a 1:1 ratio in the presence or absence of Ultra-LEAF Purified anti-human HLA-A,B,C (BioLegend) for 96 h. At the end of the co-culture, cells were harvested, washed, and stained using CD4 (BioLegend), CD8 (BioLegend), LIVE/DEAD Aqua (Thermo Fisher Scientific), HLADR (BioLegend), and anti-mouse TCRb (binds to E7 TCR; BioLegend).

    Isolation:

    Article Title: Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti-tumor responses and phagocytic uptake of tumor-associated antigen for T cell cross-presentation.
    Article Snippet: Parallel plates were prepared for supernatant collection at 120 h. Cytokine secretion was measured using the Ella automated immunoassay platform (Biotechne, Minneapolis, MN). .. SCC152 E7-expressing TMEM30A KO cells were trypsinized, counted, and plated for 72 h at 37 C. CER-1236 or control T cells were added to each well in the presence or absence of the TLR2 inhibitor C29 and co-cultured for 24 h at 37 C. At the end of co-culture, cells were resuspended in autoMACS Rinsing Solution (Miltenyi Biotec) and CD3+ cells were isolated using a CD3 Positive Selection Kit (Miltenyi Biotec). .. CER-1236 or control T cells were counted, then cocultured with CTV-labeled E7 TCR T cells at a 1:1 ratio in the presence or absence of Ultra-LEAF Purified anti-human HLA-A,B,C (BioLegend) for 96 h. At the end of the co-culture, cells were harvested, washed, and stained using CD4 (BioLegend), CD8 (BioLegend), LIVE/DEAD Aqua (Thermo Fisher Scientific), HLADR (BioLegend), and anti-mouse TCRb (binds to E7 TCR; BioLegend).

    Article Title: Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti- tumor responses and phagocytic uptake of tumor- associated antigen for T cell cross-presentation
    Article Snippet: Parallel plates were prepared for supernatant collection at 120 h. Cytokine secretion was measured using the Ella automated immunoassay platform (Biotechne, Minneapolis, MN). .. SCC152 E7-expressing TMEM30A KO cells were trypsinized, counted, and plated for 72 h at 37 C. CER-1236 or control T cells were added to each well in the presence or absence of the TLR2 inhibitor C29 and co-cultured for 24 h at 37 C. At the end of co-culture, cells were resuspended in autoMACS Rinsing Solution (Miltenyi Biotec) and CD3+ cells were isolated using a CD3 Positive Selection Kit (Miltenyi Biotec). .. CER-1236 or control T cells were counted, then cocultured with CTV-labeled E7 TCR T cells at a 1:1 ratio in the presence or absence of Ultra-LEAF Purified anti-human HLA-A,B,C (BioLegend) for 96 h. At the end of the co-culture, cells were harvested, washed, and stained using CD4 (BioLegend), CD8 (BioLegend), LIVE/DEAD Aqua (Thermo Fisher Scientific), HLADR (BioLegend), and anti-mouse TCRb (binds to E7 TCR; BioLegend).

    Selection:

    Article Title: Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti-tumor responses and phagocytic uptake of tumor-associated antigen for T cell cross-presentation.
    Article Snippet: Parallel plates were prepared for supernatant collection at 120 h. Cytokine secretion was measured using the Ella automated immunoassay platform (Biotechne, Minneapolis, MN). .. SCC152 E7-expressing TMEM30A KO cells were trypsinized, counted, and plated for 72 h at 37 C. CER-1236 or control T cells were added to each well in the presence or absence of the TLR2 inhibitor C29 and co-cultured for 24 h at 37 C. At the end of co-culture, cells were resuspended in autoMACS Rinsing Solution (Miltenyi Biotec) and CD3+ cells were isolated using a CD3 Positive Selection Kit (Miltenyi Biotec). .. CER-1236 or control T cells were counted, then cocultured with CTV-labeled E7 TCR T cells at a 1:1 ratio in the presence or absence of Ultra-LEAF Purified anti-human HLA-A,B,C (BioLegend) for 96 h. At the end of the co-culture, cells were harvested, washed, and stained using CD4 (BioLegend), CD8 (BioLegend), LIVE/DEAD Aqua (Thermo Fisher Scientific), HLADR (BioLegend), and anti-mouse TCRb (binds to E7 TCR; BioLegend).

    Article Title: FcαRI (CD89) is upregulated on subsets of mucosal and circulating NK cells and regulates IgA-class specific signaling and functions
    Article Snippet: .. Samples were aspirated and CD3 + cells were depleted using Miltenyi Human CD3 Positive Selection Kit and Miltenyi NHP CD3 Positive Selection Kit for human and RM, respectively, according to the manufacturer’s protocol. ..

    Article Title: EACR 2025 Congress: Innovative Cancer Science, 16-19 June 2025.
    Article Snippet: .. With the MACSprepTM CD3 and CD19 Selection Kit, Miltenyi Biotec offers a new cell separation workflow to generate T and B cells from limited human blood samples in manual and automated processes. ..

    Article Title: FcαRI (CD89) is upregulated on subsets of mucosal and circulating NK cells and regulates IgA-class specific signaling and functions.
    Article Snippet: .. Samples were aspirated and CD3+ cells were depleted using Miltenyi Human CD3 Positive Selection Kit and Miltenyi NHP CD3 Positive Selection Kit (Miltenyi Biotec, Charlestown, MA, USA) for human and RM, respectively, according to the manufacturer’s protocol. ..

    Article Title: Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti- tumor responses and phagocytic uptake of tumor- associated antigen for T cell cross-presentation
    Article Snippet: Parallel plates were prepared for supernatant collection at 120 h. Cytokine secretion was measured using the Ella automated immunoassay platform (Biotechne, Minneapolis, MN). .. SCC152 E7-expressing TMEM30A KO cells were trypsinized, counted, and plated for 72 h at 37 C. CER-1236 or control T cells were added to each well in the presence or absence of the TLR2 inhibitor C29 and co-cultured for 24 h at 37 C. At the end of co-culture, cells were resuspended in autoMACS Rinsing Solution (Miltenyi Biotec) and CD3+ cells were isolated using a CD3 Positive Selection Kit (Miltenyi Biotec). .. CER-1236 or control T cells were counted, then cocultured with CTV-labeled E7 TCR T cells at a 1:1 ratio in the presence or absence of Ultra-LEAF Purified anti-human HLA-A,B,C (BioLegend) for 96 h. At the end of the co-culture, cells were harvested, washed, and stained using CD4 (BioLegend), CD8 (BioLegend), LIVE/DEAD Aqua (Thermo Fisher Scientific), HLADR (BioLegend), and anti-mouse TCRb (binds to E7 TCR; BioLegend).



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    Assessment of the functional impact of neutrophils from Ax-3 crew on T cell responses (A) CD3 + T cells isolated from the peripheral blood of the astronauts before and after the mission were stimulated with anti-CD3/28 beads and proliferation responses were measured by a CFSE-based flow cytometric assay. Histograms obtained from an astronaut are shown as a representative result. T cells from healthy volunteers (ground controls) were also studied (gray histogram). (B) A schematic (created in BioRender Created in BioRender. Esendagli, G. (2025) https://BioRender.com/a4m1kdr ) is presented for the experimental setup used for testing the functional impact of neutrophils. NDN and LDN cells collected from astronauts before (L-1d) and after (R+1day) the Ax-3 mission were co-cultured with the anti-CD3/CD28-stimulated T cells obtained from healthy control individuals. T cells proliferation (C), and secretion of IL-2 (D) and IFN-γ (E) were determined following incubation for 72 h. Statistical analyses were performed with paired t test (∗∗ p ≤ 0.01). The p values calculated to be > 0.05 were not indicated. The data are presented as mean with standard error of mean (SEM).

    Journal: iScience

    Article Title: Spaceflight alters the immune regulatory functions of neutrophil granulocytes on T lymphocytes

    doi: 10.1016/j.isci.2025.114380

    Figure Lengend Snippet: Assessment of the functional impact of neutrophils from Ax-3 crew on T cell responses (A) CD3 + T cells isolated from the peripheral blood of the astronauts before and after the mission were stimulated with anti-CD3/28 beads and proliferation responses were measured by a CFSE-based flow cytometric assay. Histograms obtained from an astronaut are shown as a representative result. T cells from healthy volunteers (ground controls) were also studied (gray histogram). (B) A schematic (created in BioRender Created in BioRender. Esendagli, G. (2025) https://BioRender.com/a4m1kdr ) is presented for the experimental setup used for testing the functional impact of neutrophils. NDN and LDN cells collected from astronauts before (L-1d) and after (R+1day) the Ax-3 mission were co-cultured with the anti-CD3/CD28-stimulated T cells obtained from healthy control individuals. T cells proliferation (C), and secretion of IL-2 (D) and IFN-γ (E) were determined following incubation for 72 h. Statistical analyses were performed with paired t test (∗∗ p ≤ 0.01). The p values calculated to be > 0.05 were not indicated. The data are presented as mean with standard error of mean (SEM).

    Article Snippet: CD3 + T cells were isolated from peripheral blood samples of healthy donors by using Human CD3 Positive Selection Kit (Miltenyi).

    Techniques: Functional Assay, Isolation, Flow Cytometry, Cell Culture, Control, Incubation

    Comparison of the impact of space flight and suborbital flight on immunophenotypic and functional characters of NDN (A) Granularity (SSC) and size (FSC) values of NDN before and after the Ax-3 mission and the suborbital flight mission were quantified by flow cytometry. (B–D) Expression levels of the neutrophil surface molecules were calculated by normalizing the median fluorescence intensity (MFI) to AF for each marker and a comparative heatmap was prepared (A.U., arbitrary units). The change in ROS (C) and NO (D) production capacities in NDN after the Ax-3 mission and the suborbital flight was calculated by comparing the data of the NDN obtained before the mission. (E–H) NDN cells collected from the astronauts before and after the Ax-3 and suborbital flight missions were co-cultured with the anti-CD3/CD28-stimulated T cells from healthy control individuals. T cell proliferation in the co-cultures with increasing amounts of NDN was normalized to that of the T cells stimulated alone. In addition, the changes in T cell proliferation (F), and secretion of IL-2 (G) and IFN-γ (H) in the presence of NDN after the Ax-3 mission and the suborbital flight were calculated by comparing to the data obtained with the NDN collected before the missions. Statistical analyses were performed with paired t test (∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001). The p values calculated to be > 0.05 were not indicated. The data are presented as mean with standard error of mean (SEM).

    Journal: iScience

    Article Title: Spaceflight alters the immune regulatory functions of neutrophil granulocytes on T lymphocytes

    doi: 10.1016/j.isci.2025.114380

    Figure Lengend Snippet: Comparison of the impact of space flight and suborbital flight on immunophenotypic and functional characters of NDN (A) Granularity (SSC) and size (FSC) values of NDN before and after the Ax-3 mission and the suborbital flight mission were quantified by flow cytometry. (B–D) Expression levels of the neutrophil surface molecules were calculated by normalizing the median fluorescence intensity (MFI) to AF for each marker and a comparative heatmap was prepared (A.U., arbitrary units). The change in ROS (C) and NO (D) production capacities in NDN after the Ax-3 mission and the suborbital flight was calculated by comparing the data of the NDN obtained before the mission. (E–H) NDN cells collected from the astronauts before and after the Ax-3 and suborbital flight missions were co-cultured with the anti-CD3/CD28-stimulated T cells from healthy control individuals. T cell proliferation in the co-cultures with increasing amounts of NDN was normalized to that of the T cells stimulated alone. In addition, the changes in T cell proliferation (F), and secretion of IL-2 (G) and IFN-γ (H) in the presence of NDN after the Ax-3 mission and the suborbital flight were calculated by comparing to the data obtained with the NDN collected before the missions. Statistical analyses were performed with paired t test (∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001). The p values calculated to be > 0.05 were not indicated. The data are presented as mean with standard error of mean (SEM).

    Article Snippet: CD3 + T cells were isolated from peripheral blood samples of healthy donors by using Human CD3 Positive Selection Kit (Miltenyi).

    Techniques: Comparison, Functional Assay, Flow Cytometry, Expressing, Fluorescence, Marker, Cell Culture, Control

    ( A ) Schematic diagram of alloantigen-based suppression assay shown in (B) and (C). HLA-A2 + DCs were cocultured with HLA-A3 − T reg cells for 72 hours. Allogeneic CPD eFluor 450–labeled HLA-A3 + CD3 + responder T cells were then added, and cocultures were maintained for an additional 96 hours. ( B ) Representative CD4 + responder T cell proliferation, gated on live HLA-A3 + CPD eFluor 670 − CPD eFluor 450 + CD4 + cells. ( C ) % Suppression of CD4 + responder T cell proliferation, relative to responder T cells cultured without T reg cells. ( D ) Schematic diagram of islet antigen–based suppression assay shown in (F) to (H). HLA-A2 + HLA-DR4 + DCs were cocultured with T reg cells for 48 hours. CD4 + 4.13-TCR + responder T cells and 1 nM GAD65 peptide were then added, and cocultures were maintained for an additional 48 hours. ( E ) CD4 + 4.13-TCR + T cell proliferation following 96-hour coculture with immature HLA-DR4 + DCs and varying GAD65 peptide concentrations (without T reg cells). Control cells were pulsed with an irrelevant hemagglutinin peptide (100 nM). Division indices provided. ( F ) Representative CD4 + responder T cell proliferation, gated on live CD4 + CPD eFluor 670 − mTCRβ + cells. ( G ) % Suppression of responder T cell proliferation, relative to responder T cells stimulated without T reg cells. ( H ) Relative cytokine analysis from 1:128 T reg cell:responder T cell (T resp cell) ratio. TNFα, IL-17A & IL-17F were not detected. Averaged data are means ± SEM with non-linear regression lines ( n = 8 to 10). Statistical significance was determined using mixed-effects analysis with P values shown. n.s., not significant.

    Journal: Science Advances

    Article Title: Armored human CAR T reg cells with PD1 promoter-driven IL-10 have enhanced suppressive function

    doi: 10.1126/sciadv.adx7845

    Figure Lengend Snippet: ( A ) Schematic diagram of alloantigen-based suppression assay shown in (B) and (C). HLA-A2 + DCs were cocultured with HLA-A3 − T reg cells for 72 hours. Allogeneic CPD eFluor 450–labeled HLA-A3 + CD3 + responder T cells were then added, and cocultures were maintained for an additional 96 hours. ( B ) Representative CD4 + responder T cell proliferation, gated on live HLA-A3 + CPD eFluor 670 − CPD eFluor 450 + CD4 + cells. ( C ) % Suppression of CD4 + responder T cell proliferation, relative to responder T cells cultured without T reg cells. ( D ) Schematic diagram of islet antigen–based suppression assay shown in (F) to (H). HLA-A2 + HLA-DR4 + DCs were cocultured with T reg cells for 48 hours. CD4 + 4.13-TCR + responder T cells and 1 nM GAD65 peptide were then added, and cocultures were maintained for an additional 48 hours. ( E ) CD4 + 4.13-TCR + T cell proliferation following 96-hour coculture with immature HLA-DR4 + DCs and varying GAD65 peptide concentrations (without T reg cells). Control cells were pulsed with an irrelevant hemagglutinin peptide (100 nM). Division indices provided. ( F ) Representative CD4 + responder T cell proliferation, gated on live CD4 + CPD eFluor 670 − mTCRβ + cells. ( G ) % Suppression of responder T cell proliferation, relative to responder T cells stimulated without T reg cells. ( H ) Relative cytokine analysis from 1:128 T reg cell:responder T cell (T resp cell) ratio. TNFα, IL-17A & IL-17F were not detected. Averaged data are means ± SEM with non-linear regression lines ( n = 8 to 10). Statistical significance was determined using mixed-effects analysis with P values shown. n.s., not significant.

    Article Snippet: After 3 days, CD3 + responder T cells (previously enriched from an HLA-A3 + individual using the EasySep Human CD3 Positive Selection Kit II; STEMCELL Technologies) were labeled with CPD eFluor 450 and added to the cocultures at a 5:1 responder T cell:DC ratio.

    Techniques: Suppression Assay, Labeling, Cell Culture, Control