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rabbit anti tlr2 antibody  (Proteintech)


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    Structured Review

    Proteintech rabbit anti tlr2 antibody
    Nr-CWS affects MARCO expression through TLR4. The expression of TLR4 significantly increased after Nr-CWS treatment [ (D-G) , scale bars = 100 μm], while the expression <t>TLR2</t> was unchanged (A-C) . Subsequent inhibition of TLR4 expression resulted in decreased levels of MARCO [ (H-J) , scale bars = 100 μm]. n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001.
    Rabbit Anti Tlr2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd282/TLR2+Antibody/pmc12936005-236-71-80
    Average 94 stars, based on 117 article reviews
    rabbit anti tlr2 antibody - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Nocardia rubra cell wall skeleton-induced MARCO expression: implications for improved phagocytosis and cytokine secretion in tumor-associated macrophages"

    Article Title: Nocardia rubra cell wall skeleton-induced MARCO expression: implications for improved phagocytosis and cytokine secretion in tumor-associated macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1611476

    Nr-CWS affects MARCO expression through TLR4. The expression of TLR4 significantly increased after Nr-CWS treatment [ (D-G) , scale bars = 100 μm], while the expression TLR2 was unchanged (A-C) . Subsequent inhibition of TLR4 expression resulted in decreased levels of MARCO [ (H-J) , scale bars = 100 μm]. n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: Nr-CWS affects MARCO expression through TLR4. The expression of TLR4 significantly increased after Nr-CWS treatment [ (D-G) , scale bars = 100 μm], while the expression TLR2 was unchanged (A-C) . Subsequent inhibition of TLR4 expression resulted in decreased levels of MARCO [ (H-J) , scale bars = 100 μm]. n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Expressing, Inhibition

    Related Articles

    Western Blot:

    Article Title: Nocardia rubra cell wall skeleton-induced MARCO expression: implications for improved phagocytosis and cytokine secretion in tumor-associated macrophages
    Article Snippet: .. The following commercial antibodies (vendor, catalog number and dilution) utilized for western blot and immunohistochemical staining were employed in accordance with the manufacturers’ guidelines: mouse anti-CD68 antibody (Abcam, ab201340, 1:200), rabbit anti-MARCO antibody (Abcam, ab231046, 1:1000 for western blot and 1:100 for immunohistochemistry), rabbit anti-CD163 antibody (Abways Technology, CY6845, 1:500), mouse anti-CD86 antibody (Proteintech, 68674-2-Ig, 1:5000), mouse anti-TLR4 antibody (Santa Cruz Biotechnology, sc-293072, 1:1000 for western blot and 1:200 for immunohistochemistry), rabbit anti-TLR2 antibody(Abways Technology, CY5102, 1:2000), mouse anti-GAPDH antibody (Proteintech, 6004-1-Ig, 1:50000), HRP-goat anti-mouse recombinant secondary antibody (H+L)(Proteintech, RGAM001, 1:5000), goat anti-mouse IgG (H+L) Alexa Fluor 594 (Abways Technology, AB0152, 1:300), HRP-goat anti-rabbit recombinant secondary antibody (H+L) (Proteintech, RGAR001, 1:5000), goat anti-rabbit IgG (H+L) secondary antibody DyLightTM 594 (Report Biotech, S7002, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 488 (Report Biotech, S6001, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 594 (Abways Technology, AB0152, 1:500). ..

    Article Title: CNPY3 variants associated with a recessive developmental and epileptic encephalopathy affect inflammasome and TLR function
    Article Snippet: Antibodies for flow cytometry: Anti-TLR2 (Miltenyi Biotec, Cat#130-120-138, RRID: AB_2752006 ) and corresponding isotype control (Miltenyi Biotec, Cat#130-113-446, RRID: AB_2733446 ). .. Antibodies for Western blot: CNPY3 (Proteintech, Cat#15215-1-AP, RRID: AB_11182172 ), TLR2 (Proteintech, Cat66645, RRID: AB_3084262 ), FLT3 clone C-20 (Santa Cruz Biotech), HA-tag (Biolegend Cat#901513, RRID: AB_2565335 ). .. Secondary Antibodies IRDye 680RD Goat-anti-Rabbit (LICORbio Cat# 925-68071, RRID: AB_2721181 ) and IRDye 800CW Goat-anti-Mouse (LICORbio Cat#926-32210, RRID: AB_621842 ) for immunofluorescent detection, antibody diluent and blocking solutions (LI-COR Biotech, Lincoln, NE, USA).

    Immunohistochemical staining:

    Article Title: Nocardia rubra cell wall skeleton-induced MARCO expression: implications for improved phagocytosis and cytokine secretion in tumor-associated macrophages
    Article Snippet: .. The following commercial antibodies (vendor, catalog number and dilution) utilized for western blot and immunohistochemical staining were employed in accordance with the manufacturers’ guidelines: mouse anti-CD68 antibody (Abcam, ab201340, 1:200), rabbit anti-MARCO antibody (Abcam, ab231046, 1:1000 for western blot and 1:100 for immunohistochemistry), rabbit anti-CD163 antibody (Abways Technology, CY6845, 1:500), mouse anti-CD86 antibody (Proteintech, 68674-2-Ig, 1:5000), mouse anti-TLR4 antibody (Santa Cruz Biotechnology, sc-293072, 1:1000 for western blot and 1:200 for immunohistochemistry), rabbit anti-TLR2 antibody(Abways Technology, CY5102, 1:2000), mouse anti-GAPDH antibody (Proteintech, 6004-1-Ig, 1:50000), HRP-goat anti-mouse recombinant secondary antibody (H+L)(Proteintech, RGAM001, 1:5000), goat anti-mouse IgG (H+L) Alexa Fluor 594 (Abways Technology, AB0152, 1:300), HRP-goat anti-rabbit recombinant secondary antibody (H+L) (Proteintech, RGAR001, 1:5000), goat anti-rabbit IgG (H+L) secondary antibody DyLightTM 594 (Report Biotech, S7002, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 488 (Report Biotech, S6001, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 594 (Abways Technology, AB0152, 1:500). ..

    Staining:

    Article Title: Nocardia rubra cell wall skeleton-induced MARCO expression: implications for improved phagocytosis and cytokine secretion in tumor-associated macrophages
    Article Snippet: .. The following commercial antibodies (vendor, catalog number and dilution) utilized for western blot and immunohistochemical staining were employed in accordance with the manufacturers’ guidelines: mouse anti-CD68 antibody (Abcam, ab201340, 1:200), rabbit anti-MARCO antibody (Abcam, ab231046, 1:1000 for western blot and 1:100 for immunohistochemistry), rabbit anti-CD163 antibody (Abways Technology, CY6845, 1:500), mouse anti-CD86 antibody (Proteintech, 68674-2-Ig, 1:5000), mouse anti-TLR4 antibody (Santa Cruz Biotechnology, sc-293072, 1:1000 for western blot and 1:200 for immunohistochemistry), rabbit anti-TLR2 antibody(Abways Technology, CY5102, 1:2000), mouse anti-GAPDH antibody (Proteintech, 6004-1-Ig, 1:50000), HRP-goat anti-mouse recombinant secondary antibody (H+L)(Proteintech, RGAM001, 1:5000), goat anti-mouse IgG (H+L) Alexa Fluor 594 (Abways Technology, AB0152, 1:300), HRP-goat anti-rabbit recombinant secondary antibody (H+L) (Proteintech, RGAR001, 1:5000), goat anti-rabbit IgG (H+L) secondary antibody DyLightTM 594 (Report Biotech, S7002, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 488 (Report Biotech, S6001, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 594 (Abways Technology, AB0152, 1:500). ..

    Immunohistochemistry:

    Article Title: Nocardia rubra cell wall skeleton-induced MARCO expression: implications for improved phagocytosis and cytokine secretion in tumor-associated macrophages
    Article Snippet: .. The following commercial antibodies (vendor, catalog number and dilution) utilized for western blot and immunohistochemical staining were employed in accordance with the manufacturers’ guidelines: mouse anti-CD68 antibody (Abcam, ab201340, 1:200), rabbit anti-MARCO antibody (Abcam, ab231046, 1:1000 for western blot and 1:100 for immunohistochemistry), rabbit anti-CD163 antibody (Abways Technology, CY6845, 1:500), mouse anti-CD86 antibody (Proteintech, 68674-2-Ig, 1:5000), mouse anti-TLR4 antibody (Santa Cruz Biotechnology, sc-293072, 1:1000 for western blot and 1:200 for immunohistochemistry), rabbit anti-TLR2 antibody(Abways Technology, CY5102, 1:2000), mouse anti-GAPDH antibody (Proteintech, 6004-1-Ig, 1:50000), HRP-goat anti-mouse recombinant secondary antibody (H+L)(Proteintech, RGAM001, 1:5000), goat anti-mouse IgG (H+L) Alexa Fluor 594 (Abways Technology, AB0152, 1:300), HRP-goat anti-rabbit recombinant secondary antibody (H+L) (Proteintech, RGAR001, 1:5000), goat anti-rabbit IgG (H+L) secondary antibody DyLightTM 594 (Report Biotech, S7002, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 488 (Report Biotech, S6001, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 594 (Abways Technology, AB0152, 1:500). ..

    Recombinant:

    Article Title: Nocardia rubra cell wall skeleton-induced MARCO expression: implications for improved phagocytosis and cytokine secretion in tumor-associated macrophages
    Article Snippet: .. The following commercial antibodies (vendor, catalog number and dilution) utilized for western blot and immunohistochemical staining were employed in accordance with the manufacturers’ guidelines: mouse anti-CD68 antibody (Abcam, ab201340, 1:200), rabbit anti-MARCO antibody (Abcam, ab231046, 1:1000 for western blot and 1:100 for immunohistochemistry), rabbit anti-CD163 antibody (Abways Technology, CY6845, 1:500), mouse anti-CD86 antibody (Proteintech, 68674-2-Ig, 1:5000), mouse anti-TLR4 antibody (Santa Cruz Biotechnology, sc-293072, 1:1000 for western blot and 1:200 for immunohistochemistry), rabbit anti-TLR2 antibody(Abways Technology, CY5102, 1:2000), mouse anti-GAPDH antibody (Proteintech, 6004-1-Ig, 1:50000), HRP-goat anti-mouse recombinant secondary antibody (H+L)(Proteintech, RGAM001, 1:5000), goat anti-mouse IgG (H+L) Alexa Fluor 594 (Abways Technology, AB0152, 1:300), HRP-goat anti-rabbit recombinant secondary antibody (H+L) (Proteintech, RGAR001, 1:5000), goat anti-rabbit IgG (H+L) secondary antibody DyLightTM 594 (Report Biotech, S7002, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 488 (Report Biotech, S6001, 1:500), goat anti-mouse IgG (H+L) secondary antibody DyLightTM 594 (Abways Technology, AB0152, 1:500). ..

    Microscale Thermophoresis:

    Article Title: Bushen Huoxue Recipe enhances the immune microenvironment at the maternal-fetal interface via the Hippo signaling pathway in mice with recurrent spontaneous abortion
    Article Snippet: A PrimeScript RT reagent kit (RR047A) was purchased from TaKaRa Bio (Beijing, China), and PCR primers were purchased from Sangon Biotech (Shanghai, China). .. Antibodies against TLR2 (66645-1-Ig), TLR4 (66350-1-Ig), TLR7 (17232-1-AP), and GAPDH (60004-1-Ig) were purchased from Proteintech (Rosemont, IL, USA); antibodies against RORγt (ab207082) and FoxP3 (ab215206) were purchased from Abcam (Cambridge, MA, USA); Alexa Fluor 555 nm (8953) and antibodies against MST (14946), p-MST (49332), LATS (3477S), p-LATS (8654S), YAP (14074S), p-YAP (13008S), TAZ (83669S), and p-TAZ (59971S) were purchased from Cell Signaling Technology (Danvers, MA, USA); and an antibody against Lamin B (A11495) was purchased from ABclonal (Wuhan, China). ..

    Cell-Signaling:

    Article Title: Bushen Huoxue Recipe enhances the immune microenvironment at the maternal-fetal interface via the Hippo signaling pathway in mice with recurrent spontaneous abortion
    Article Snippet: A PrimeScript RT reagent kit (RR047A) was purchased from TaKaRa Bio (Beijing, China), and PCR primers were purchased from Sangon Biotech (Shanghai, China). .. Antibodies against TLR2 (66645-1-Ig), TLR4 (66350-1-Ig), TLR7 (17232-1-AP), and GAPDH (60004-1-Ig) were purchased from Proteintech (Rosemont, IL, USA); antibodies against RORγt (ab207082) and FoxP3 (ab215206) were purchased from Abcam (Cambridge, MA, USA); Alexa Fluor 555 nm (8953) and antibodies against MST (14946), p-MST (49332), LATS (3477S), p-LATS (8654S), YAP (14074S), p-YAP (13008S), TAZ (83669S), and p-TAZ (59971S) were purchased from Cell Signaling Technology (Danvers, MA, USA); and an antibody against Lamin B (A11495) was purchased from ABclonal (Wuhan, China). ..

    Hemagglutination Assay:

    Article Title: CNPY3 variants associated with a recessive developmental and epileptic encephalopathy affect inflammasome and TLR function
    Article Snippet: Antibodies for flow cytometry: Anti-TLR2 (Miltenyi Biotec, Cat#130-120-138, RRID: AB_2752006 ) and corresponding isotype control (Miltenyi Biotec, Cat#130-113-446, RRID: AB_2733446 ). .. Antibodies for Western blot: CNPY3 (Proteintech, Cat#15215-1-AP, RRID: AB_11182172 ), TLR2 (Proteintech, Cat66645, RRID: AB_3084262 ), FLT3 clone C-20 (Santa Cruz Biotech), HA-tag (Biolegend Cat#901513, RRID: AB_2565335 ). .. Secondary Antibodies IRDye 680RD Goat-anti-Rabbit (LICORbio Cat# 925-68071, RRID: AB_2721181 ) and IRDye 800CW Goat-anti-Mouse (LICORbio Cat#926-32210, RRID: AB_621842 ) for immunofluorescent detection, antibody diluent and blocking solutions (LI-COR Biotech, Lincoln, NE, USA).

    other:

    Article Title: Protective effect of Zymosan-A against radiation-induced premature ovarian insufficiency in a murine model
    Article Snippet: Sequencing was conducted on the Illumina HiSeq 2500 platform at Oebiotech (Shanghai, China).



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