Review



cd25 apc  (Elabscience Biotechnology)


Bioz Verified Symbol Elabscience Biotechnology is a verified supplier
Bioz Manufacturer Symbol Elabscience Biotechnology manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Elabscience Biotechnology cd25 apc
    Cd25 Apc, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/APC+Anti-Mouse+CD25+Antibody/pmc12840754-251-89-90
    Average 94 stars, based on 43 article reviews
    cd25 apc - by Bioz Stars, 2026-11
    94/100 stars

    Images

    Related Articles

    other:

    Article Title: Cyclophosphamide-Mediated Induction of Myeloid-Derived Suppressor Cells In Vivo: Kinetics of Accumulation, Immune Profile, and Immunomodulation by Oleanane-Type Triterpenoids
    Article Snippet: Monoclonal anti-mouse antibodies: anti-CD16/CD32 (BD Biosciences, #553142, clone 2.4G2, San Diego, CA, USA), CD45-FITC (BD Biosciences, #553080, clone 30-F11, San Diego, CA, USA), CD11b-PerCP (Elabscience, #E-AB-F1081UF, clone M1/70, Houston, TX, USA), Ly6C-BV605 (Sony, #1240180, clone HK1.4, San Jose, CA, USA), Ly6G-PE (Elabscience, #E-AB-F1108UD, clone 1A8, Houston, TX, USA), MHCII-FITC (Elabscience, #E-AB-F0990UC, clone M5/114, Houston, TX, USA), F4/80-PB (Sony, #1215615, clone BM8, San Jose, CA, USA), CD3-PE-Cy7 (Elabscience, #E-AB-F1013H, clone 17A2, Houston, TX, USA), CD4-PE (Elabscience, #E-AB-F1097D, clone GK1.5, Houston, TX, USA), CD8-PerCP (Elabscience, #E-AB-F1104F, clone 53-6.7, Houston, TX, USA), CD25-APC (Elabscience, #E-AB-F1102E, clone PC-61.5.3, Houston, TX, USA), Ly-6G/Ly-6C (Gr-1) (Elabscience, Ca#E-AB-F1120A, clone RB6-8C5, Houston, TX, USA), PD-L1-APC (Elabscience, #E-AB-F1132E, clone 10F.9G2, Houston, TX, USA).

    Article Title: Gallic acid/copper ion-based metal-phenolic networks as photothermal-enhanced nanocatalysts for cancer therapy
    Article Snippet: HKOH-1 and Monobromobimane (MBB) were acquired from MedChem Express (Shanghai, China).

    Article Title: Cyclophosphamide-Mediated Induction of Myeloid-Derived Suppressor Cells In Vivo: Kinetics of Accumulation, Immune Profile, and Immunomodulation by Oleanane-Type Triterpenoids.
    Article Snippet: Monoclonal anti-mouse antibodies: anti-CD16/CD32 (BD Biosciences, #553142, clone 2.4G2, San Diego, CA, USA), CD45-FITC (BD Biosciences, #553080, clone 30-F11, San Diego, CA, USA), CD11b-PerCP (Elabscience, #E-AB-F1081UF, clone M1/70, Houston, TX, USA), Ly6C-BV605 (Sony, #1240180, clone HK1.4, San Jose, CA, USA), Ly6G-PE (Elabscience, #E-AB-F1108UD, clone 1A8, Houston, TX, USA), MHCII-FITC (Elabscience, #EAB-F0990UC, clone M5/114, Houston, TX, USA), F4/80-PB (Sony, #1215615, clone BM8, San Jose, CA, USA), CD3-PE-Cy7 (Elabscience, #E-AB-F1013H, clone 17A2, Houston, TX, USA), CD4-PE (Elabscience, #E-AB-F1097D, clone GK1.5, Houston, TX, USA), CD8-PerCP (Elabscience, #E-AB-F1104F, clone 53-6.7, Houston, TX, USA), CD25-APC (Elabscience, #E-AB-F1102E, clone PC-61.5.3, Houston, TX, USA), Ly-6G/Ly-6C (Gr-1) (Elabscience, Ca#E-AB-F1120A, clone RB6-8C5, Houston, TX, USA), PD-L1-APC (Elabscience, #E-ABF1132E, clone 10F.9G2, Houston, TX, USA).

    Activation Assay:

    Article Title: Local therapeutic platform prevents postsurgical GBM recurrence by diminishing GICs and reshaping immunosuppressive microenvironment
    Article Snippet: .. The following antibody panels were used: anti-CD3-FITC (E-AB-F1013C, Elabscience), anti-CD4-PE (E-AB-F1097D, Elabscience), and anti-CD8-Percp Cy5.5 (E-AB-F1104J, Elabscience) for CD4 + /CD8 + T-cell analysis; anti-CD11c-FITC (E-AB-F0991C, Elabscience), anti-CD80-APC (E-AB-F0992E, Elabscience), and anti-CD86-PE (E-AB-F0994D, Elabscience) for DC cell detection; anti-CD3-FITC (E-AB-F1013C, Elabscience), anti-CD25-APC (E-AB-F1102E, Elabscience), and anti-CD69-PE (E-AB-F1187D, Elabscience) for T-cell activation assessment; anti-CD3-Percp Cy5.5 (E-AB-F1013J, Elabscience), anti-CD4-FITC (E-AB-F1353C, Elabscience), and anti-Foxp3-PE (E-AB-F1238D, Elabscience) for Treg cell analysis; and anti-CD11b-FITC (E-AB-F1081C, Elabscience) and anti-Gr-1-PE (E-AB-F1120D, Elabscience) for MDSC detection. .. The secretion levels of IFN-γ (E-EL-M0048c), TNF-α (E-EL-M3063), IL-4 (E-EL-M0043c), and IL-10 (E-EL-M0046c) in brain tumor tissues were determined by ELISA (Elabscience).

    Article Title: Local therapeutic platform prevents postsurgical GBM recurrence by diminishing GICs and reshaping immunosuppressive microenvironment.
    Article Snippet: .. The following antibody panels were used: anti-CD3-FITC (E-AB-F1013C, Elabscience), anti-CD4-PE (E-AB-F1097D, Elabscience), and anti-CD8-Percp Cy5.5 (E-AB-F1104J, Elabscience) for CD4+/CD8+ T-cell analysis; antiAR TI CL E IN P RE SS CD11c-FITC (E-AB-F0991C, Elabscience), anti-CD80-APC (E-AB-F0992E, Elabscience), and anti-CD86-PE (E-AB-F0994D, Elabscience) for DC cell detection; anti-CD3-FITC (E-ABF1013C, Elabscience), anti-CD25-APC (E-AB-F1102E, Elabscience), and anti-CD69-PE (E-ABF1187D, Elabscience) for T-cell activation assessment; anti-CD3-Percp Cy5.5 (E-AB-F1013J, Elabscience), anti-CD4-FITC (E-AB-F1353C, Elabscience), and anti-Foxp3-PE (E-AB-F1238D, Elabscience) for Treg cell analysis; and anti-CD11b-FITC (E-AB-F1081C, Elabscience) and antiGr-1-PE (E-AB-F1120D, Elabscience) for MDSC detection. .. The secretion levels of IFN-γ (E-ELM0048c), TNF-α (E-EL-M3063), IL-4 (E-EL-M0043c), and IL-10 (E-EL-M0046c) in brain tumor tissues were determined by ELISA (Elabscience).

    Cell Analysis:

    Article Title: Local therapeutic platform prevents postsurgical GBM recurrence by diminishing GICs and reshaping immunosuppressive microenvironment
    Article Snippet: .. The following antibody panels were used: anti-CD3-FITC (E-AB-F1013C, Elabscience), anti-CD4-PE (E-AB-F1097D, Elabscience), and anti-CD8-Percp Cy5.5 (E-AB-F1104J, Elabscience) for CD4 + /CD8 + T-cell analysis; anti-CD11c-FITC (E-AB-F0991C, Elabscience), anti-CD80-APC (E-AB-F0992E, Elabscience), and anti-CD86-PE (E-AB-F0994D, Elabscience) for DC cell detection; anti-CD3-FITC (E-AB-F1013C, Elabscience), anti-CD25-APC (E-AB-F1102E, Elabscience), and anti-CD69-PE (E-AB-F1187D, Elabscience) for T-cell activation assessment; anti-CD3-Percp Cy5.5 (E-AB-F1013J, Elabscience), anti-CD4-FITC (E-AB-F1353C, Elabscience), and anti-Foxp3-PE (E-AB-F1238D, Elabscience) for Treg cell analysis; and anti-CD11b-FITC (E-AB-F1081C, Elabscience) and anti-Gr-1-PE (E-AB-F1120D, Elabscience) for MDSC detection. .. The secretion levels of IFN-γ (E-EL-M0048c), TNF-α (E-EL-M3063), IL-4 (E-EL-M0043c), and IL-10 (E-EL-M0046c) in brain tumor tissues were determined by ELISA (Elabscience).

    Article Title: Local therapeutic platform prevents postsurgical GBM recurrence by diminishing GICs and reshaping immunosuppressive microenvironment.
    Article Snippet: .. The following antibody panels were used: anti-CD3-FITC (E-AB-F1013C, Elabscience), anti-CD4-PE (E-AB-F1097D, Elabscience), and anti-CD8-Percp Cy5.5 (E-AB-F1104J, Elabscience) for CD4+/CD8+ T-cell analysis; antiAR TI CL E IN P RE SS CD11c-FITC (E-AB-F0991C, Elabscience), anti-CD80-APC (E-AB-F0992E, Elabscience), and anti-CD86-PE (E-AB-F0994D, Elabscience) for DC cell detection; anti-CD3-FITC (E-ABF1013C, Elabscience), anti-CD25-APC (E-AB-F1102E, Elabscience), and anti-CD69-PE (E-ABF1187D, Elabscience) for T-cell activation assessment; anti-CD3-Percp Cy5.5 (E-AB-F1013J, Elabscience), anti-CD4-FITC (E-AB-F1353C, Elabscience), and anti-Foxp3-PE (E-AB-F1238D, Elabscience) for Treg cell analysis; and anti-CD11b-FITC (E-AB-F1081C, Elabscience) and antiGr-1-PE (E-AB-F1120D, Elabscience) for MDSC detection. .. The secretion levels of IFN-γ (E-ELM0048c), TNF-α (E-EL-M3063), IL-4 (E-EL-M0043c), and IL-10 (E-EL-M0046c) in brain tumor tissues were determined by ELISA (Elabscience).

    Staining:

    Article Title: Cancer Immunomodulatory Effect of Bidens pilosa L. in Mice: Suppression of Tumor-Associated Macrophages and Regulatory T Cells
    Article Snippet: .. Induced Tregs treated with or without samples were resuspended in PBS and stained with APC anti-mouse CD25 antibody (Elabscience, Cat. E-AB-F1102E) for 30 min at 4 °C. .. Cells were then fixed and permeabilized using the Transcription Factor Buffer Set kit (BD Biosciences, Cat. 562574, Franklin Lakes, NJ, USA) according to the manufacture’s instruction.

    Article Title: Systems-level molecular and immunological evidence identifies Th17/Treg modulation as a key mechanism of CRSJ’s neuroprotection in Parkinson’s disease
    Article Snippet: After stimulation, cells were fixed and permeabilized using the FoxP3/Transcription Factor Staining Buffer Kit (LianKe, No. IC001). .. For Treg staining, cells were incubated with FITC-conjugated anti-CD4 (Elabscience, No. E-AB-F1353C), APC-conjugated anti-CD25 (Elabscience, No. E-AB-F1102E), and PE-conjugated anti-Foxp3 (Elabscience, No. E-AB-F1238D). .. For Th17 staining, cells were labeled with FITC-anti-CD4 and APC-anti-IL-17A antibodies (Elabscience, No. E-AB-F1199E).

    Article Title: Mucosal Gingipain Vaccine Based on Framework Nucleic Acid Engineering for the Treatment of Periodontitis.
    Article Snippet: Periodontitis is a chronic inflammatory disease driven by oral microbial dysbiosis and dysregulated host immunity, in which Porphyromonas gingivalis (Pg) and its lysine-specific gingipain (Kgp) are key pathogenic factors.. Although immune targeting of Kgp holds promise for interrupting disease progression, inefficient transmucosal antigen delivery and limited dendritic cell (DC) activation hinder effective mucosal and humoral immune responses.. Here, we report a sublingual mucosal nanovaccine based on tetrahedral framework nucleic acids (tFNAs) for precise DC-directed immunomodulation.

    Incubation:

    Article Title: Systems-level molecular and immunological evidence identifies Th17/Treg modulation as a key mechanism of CRSJ’s neuroprotection in Parkinson’s disease
    Article Snippet: After stimulation, cells were fixed and permeabilized using the FoxP3/Transcription Factor Staining Buffer Kit (LianKe, No. IC001). .. For Treg staining, cells were incubated with FITC-conjugated anti-CD4 (Elabscience, No. E-AB-F1353C), APC-conjugated anti-CD25 (Elabscience, No. E-AB-F1102E), and PE-conjugated anti-Foxp3 (Elabscience, No. E-AB-F1238D). .. For Th17 staining, cells were labeled with FITC-anti-CD4 and APC-anti-IL-17A antibodies (Elabscience, No. E-AB-F1199E).

    Cell Stimulation:

    Article Title: Mucosal Gingipain Vaccine Based on Framework Nucleic Acid Engineering for the Treatment of Periodontitis.
    Article Snippet: Periodontitis is a chronic inflammatory disease driven by oral microbial dysbiosis and dysregulated host immunity, in which Porphyromonas gingivalis (Pg) and its lysine-specific gingipain (Kgp) are key pathogenic factors.. Although immune targeting of Kgp holds promise for interrupting disease progression, inefficient transmucosal antigen delivery and limited dendritic cell (DC) activation hinder effective mucosal and humoral immune responses.. Here, we report a sublingual mucosal nanovaccine based on tetrahedral framework nucleic acids (tFNAs) for precise DC-directed immunomodulation.

    Control:

    Article Title: Mucosal Gingipain Vaccine Based on Framework Nucleic Acid Engineering for the Treatment of Periodontitis.
    Article Snippet: Periodontitis is a chronic inflammatory disease driven by oral microbial dysbiosis and dysregulated host immunity, in which Porphyromonas gingivalis (Pg) and its lysine-specific gingipain (Kgp) are key pathogenic factors.. Although immune targeting of Kgp holds promise for interrupting disease progression, inefficient transmucosal antigen delivery and limited dendritic cell (DC) activation hinder effective mucosal and humoral immune responses.. Here, we report a sublingual mucosal nanovaccine based on tetrahedral framework nucleic acids (tFNAs) for precise DC-directed immunomodulation.

    Lysis:

    Article Title: Mucosal Gingipain Vaccine Based on Framework Nucleic Acid Engineering for the Treatment of Periodontitis.
    Article Snippet: Periodontitis is a chronic inflammatory disease driven by oral microbial dysbiosis and dysregulated host immunity, in which Porphyromonas gingivalis (Pg) and its lysine-specific gingipain (Kgp) are key pathogenic factors.. Although immune targeting of Kgp holds promise for interrupting disease progression, inefficient transmucosal antigen delivery and limited dendritic cell (DC) activation hinder effective mucosal and humoral immune responses.. Here, we report a sublingual mucosal nanovaccine based on tetrahedral framework nucleic acids (tFNAs) for precise DC-directed immunomodulation.

    Flow Cytometry:

    Article Title: Mucosal Gingipain Vaccine Based on Framework Nucleic Acid Engineering for the Treatment of Periodontitis.
    Article Snippet: Periodontitis is a chronic inflammatory disease driven by oral microbial dysbiosis and dysregulated host immunity, in which Porphyromonas gingivalis (Pg) and its lysine-specific gingipain (Kgp) are key pathogenic factors.. Although immune targeting of Kgp holds promise for interrupting disease progression, inefficient transmucosal antigen delivery and limited dendritic cell (DC) activation hinder effective mucosal and humoral immune responses.. Here, we report a sublingual mucosal nanovaccine based on tetrahedral framework nucleic acids (tFNAs) for precise DC-directed immunomodulation.

    Purification:

    Article Title: Mucosal Gingipain Vaccine Based on Framework Nucleic Acid Engineering for the Treatment of Periodontitis.
    Article Snippet: Periodontitis is a chronic inflammatory disease driven by oral microbial dysbiosis and dysregulated host immunity, in which Porphyromonas gingivalis (Pg) and its lysine-specific gingipain (Kgp) are key pathogenic factors.. Although immune targeting of Kgp holds promise for interrupting disease progression, inefficient transmucosal antigen delivery and limited dendritic cell (DC) activation hinder effective mucosal and humoral immune responses.. Here, we report a sublingual mucosal nanovaccine based on tetrahedral framework nucleic acids (tFNAs) for precise DC-directed immunomodulation.

    Immunopeptidomics:

    Article Title: Mucosal Gingipain Vaccine Based on Framework Nucleic Acid Engineering for the Treatment of Periodontitis.
    Article Snippet: Periodontitis is a chronic inflammatory disease driven by oral microbial dysbiosis and dysregulated host immunity, in which Porphyromonas gingivalis (Pg) and its lysine-specific gingipain (Kgp) are key pathogenic factors.. Although immune targeting of Kgp holds promise for interrupting disease progression, inefficient transmucosal antigen delivery and limited dendritic cell (DC) activation hinder effective mucosal and humoral immune responses.. Here, we report a sublingual mucosal nanovaccine based on tetrahedral framework nucleic acids (tFNAs) for precise DC-directed immunomodulation.



    Similar Products

    93
    Miltenyi Biotec anti cd25 pe
    Anti Cd25 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/CD25+Antibody%2C+anti-human%2C+REAfinity/pm42897263-69-106-110
    Average 93 stars, based on 1 article reviews
    anti cd25 pe - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    94
    MedChemExpress anti il 2r antibody
    Anti Il 2r Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/Anti-Mouse+CD25%2FIL-2R%CE%B1+Antibody/10__1186_slash_s12974___026___04060___2-103-5-7
    Average 94 stars, based on 1 article reviews
    anti il 2r antibody - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    93
    Elabscience Biotechnology cd25 pe
    Cd25 Pe, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/PE+Anti-Mouse+CD25+Antibody/pm42909153-503-25-27
    Average 93 stars, based on 1 article reviews
    cd25 pe - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec regulatory t cell isolation kit
    Regulatory T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/pm42901926-652-17-22
    Average 96 stars, based on 1 article reviews
    regulatory t cell isolation kit - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec cd4 cd25 regulatory t cell isolation kit mouse
    Cd4 Cd25 Regulatory T Cell Isolation Kit Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/pm42858252-1017-135-142
    Average 96 stars, based on 1 article reviews
    cd4 cd25 regulatory t cell isolation kit mouse - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec cd4 cd25 regulatory t cell isolation kit
    Cd4 Cd25 Regulatory T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+human/pm42768744-76-14-23
    Average 96 stars, based on 1 article reviews
    cd4 cd25 regulatory t cell isolation kit - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec human cd4 cd25 regulatory t cell isolation kit
    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. <t>CD4+CD25high</t> T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.
    Human Cd4 Cd25 Regulatory T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+human/pmc13627347-100-19-29
    Average 96 stars, based on 1 article reviews
    human cd4 cd25 regulatory t cell isolation kit - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    Image Search Results


    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Control, Lysis, Flow Cytometry, Staining, In Vitro, Isolation, Incubation, Expressing, Comparison

    PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Analysis, Activation Assay, Immunohistochemistry, Expressing

    PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Tissue, Microinjection, Injection, Staining, Analysis, Expressing, Activity Assay