20634 1 ap (Proteintech)

Proteintech is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Proteintech 20634 1 ap
20634 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd182/CXCR2+Antibody/pmc13045360-16-5-3
Average 95 stars, based on 57 article reviews

20634 1 ap - by Bioz Stars, 2026-11

95/100 stars

Images

Immunohistochemistry:

Article Title: Tumor-Associated Macrophage-Derived CXCL1 Promotes Endometrial Cancer Progression Through the CXCR2/NF-κB Pathway.
Article Snippet: Subsequently, recombinant human CXCL1 (rCXCL1; MedChemExpress, USA), CXCL1 neutralizing antibody (CXCL1- Nab; R&D Systems, USA), or the NF- κB inhibitor BAY 11- 7082 (MedChemExpress, USA) was introduced into the TABLE 2 | Antibodies used in this study. .. Application Antibody Source Catalog number Clone number Dilution IHC CD163 Proteintech 16646- 1- AP 9332 1:2000 CXCL1 Proteintech 12335- 1- AP 2919 1:200 CXCR2 Proteintech 20634- 1- AP 3579 1:200 E- cadherin Proteintech 20874- 1- AP 999 1:10000 Vimentin Proteintech 10366- 1- AP 7431 1:5000 Phospho- NF- κB p65 Proteintech 82335- 1- RR 5970 1:200 IF CXCL1 Proteintech 12335- 1- AP 2919 1:50 CXCR2 Proteintech 20634- 1- AP 3579 1:50 CD163 Proteintech 16646- 1- AP 9332 1:500 E- cadherin Proteintech 20874- 1- AP 999 1:500 NF- κB p65 Proteintech 10745- 1- AP 5970 1:200 WB CD68 Abcam AB955 KP1 1:1000 CD163 Proteintech 16646- 1- AP 9332 1:1000 CD206 Proteintech 18704- 1- AP 4360 1:500 IL10 Abways CY6705 25A3 1:1000 E- cadherin Proteintech 20874- 1- AP 999 1:50000 Vimentin Proteintech 10366- 1- AP 7431 1:6000 N- cadherin Proteintech 22018- 1- AP 1000 1:6000 CXCR2 Proteintech 20634- 1- AP 3579 1:2500 Phospho- NF- κB p65 Cell signaling technology #3033 93H1 1:1000 NF- κB p65 Proteintech 10745- 1- AP 5970 1:3000 Phospho- IκBα Cell signaling technology #2859 14D4 1:1000 IκBα Proteintech 10268- 1- AP 4792 1:20000 Phospho- IKK α/β Cell signaling technology #2697 16A6 1:1000 IKKα Zenbio R24674 1147 1:1000 IKKβ Zenbio R24677 3553 1:1000 Anti- beta Actin Proteintech 66009- 1- Ig 60 1:20000 GAPDH Proteintech 60004- 1- Ig 2597 1:20000 Abbreviations: IF, immunofluorescence; IHC, immunohistochemical; WB, Western blot. ..

Western Blot:

Immunofluorescence:

Immunohistochemical staining:

other:

Article Title: Multi-omics study on tumor-associated macrophages remodeling the tumor microenvironment via the CXCL5-CXCR2 axis to drive immune escape in bladder cancer
Article Snippet: CXCR2 , Rabbit monoclonal , Proteintech , 1:1000.

Article Title: Tumor‐Associated Macrophage‐Derived CXCL1 Promotes Endometrial Cancer Progression Through the CXCR2/NF‐κB Pathway
Article Snippet: , CXCR2 , Proteintech , 20634‐1‐AP , 3579 , 1:50.

Incubation:

Article Title: Chronic stress activates a hypothalamic vagal circuit to promote hepatocellular carcinoma progression in male mice
Article Snippet: Protein samples were separated by SDS‒PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). .. The membranes were blocked with 5% milk in TBST for 1 h at room temperature and then incubated with primary antibodies against COL1A1 (1:1000, HUABIO, Cat# HA722517), COL1A2 (1:1000, Proteintech, Cat# 14695-1-AP), CXCL1 (1:1000, Invitrogen, PA5-86508), p-STAT3 (1:1000, Proteintech, Cat# 28945-1-AP), STAT3 (1:1000, Proteintech, Cat# 10253-2-AP), CD44 (1:1000, Proteintech, Cat# 15675-1-AP), CHRNA9 (1:1000, Proteintech, Cat# 26025-1-AP), CXCR2 (1:1000, Proteintech, Cat# 20634-1-AP), Claudin5 (1:1000, Proteintech, Cat29767-1-AP), ZO1 (1:1000, Proteintech, Cat#21773-1-AP) and GAPDH (1:1000, Fdbio Science, Cat# FD0063) overnight at 4 °C. .. On the next day, the membraneswerewashedwith TBST 3 times and incubatedwith horseradish peroxidase (HRP)-conjugated secondary antibodies (Fdbio Science) for 1 h at room temperature.

Article Title: A SASP-related four-gene signature associated with intervertebral disc degeneration: integrated bioinformatics analysis and validation of pinocembrin as a therapeutic candidate.
Article Snippet: 1 Department of Orthopedic Surgery, The Third Affiliated Hospital, Jiangxi Medical College, First Hospital of Nanchang, Nanchang University, Nanchang, People’s Republic of China 2 Department of Orthopedic Surgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, People’s Republic of China 3 Jiangxi Provincial Key Laboratory of Spine and Spinal Cord Diseases, Nanchang 330006, People’s Republic of China 4 Department of Orthopedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of China 5 Department of Otorhinolaryngology, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China Abstract Background Cellular senescence and the senescence-associated secretory phenotype (SASP) are increasingly recognized as contributors to intervertebral disc degeneration (IVDD).. However, clinically relevant SASP-related gene signatures and therapeutic implications remain incompletely defined.. Methods Two peripheral whole-blood transcriptomic datasets from GEO (GSE124272 and GSE150408) were integrated.

Blocking Assay:

Tissue:

Article Title: Novel function of macrophage migration inhibitory factor in regulating post-infarct inflammation and the therapeutic significance
Article Snippet: The membrane was incubated with primary antibodies at 4 °C overnight followed by incubation with corresponding horseradish peroxidase-conjugated secondary antibody (1:5000, ZSGB-BIO, China), as described previously [ ]. .. Primary antibodies against MCP-1 (1:1000), CCR2 (1:1000), CXCR2 (1:1000), CXCR4 (1:1000), tissue inhibitor of metalloproteinases-4 (Timp-4, 1:1000), AT-1R (AGTR-1, 1:1000), heat shock protein 60 (HSP-60, 1:1000) and HSP-90 (1:1000) were purchased from ProteinTech. .. The primary Timp-2 (1:1000) antibodies were obtained from Santa Cruz.

Review