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human cxcr3 orf construct  (OriGene)


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    OriGene human cxcr3 orf construct
    (A) Flow cytometry analysis of <t>CXCR3</t> protein expression on a VGP melanoma cell line, C9. Cells were incubated with serum at 5% CO 2 . (B) Graph of CXCR3 protein expression on BOWES cells from 8 separate flow cytometry experiments. Cells were cultured either in normal culture conditions (far left bar), in serum-free media at 8% CO 2 for 24, 48, or 72 hrs, or in serum-free media at 8% CO 2 for 48 hrs followed by 48 hrs in normal culture conditions (far right bar). T-tests were performed on the averages for each time point, comparing treatment cells with the 0hr time point (NS = P>0.05, *** = P≤ 0.001). (C-D) Flow cytometry analysis of CXCR3 protein expression on an RGP cell line, BOWES. Cells were incubated (C) with serum at 5% CO 2 or (D) without serum at 8% CO 2 for 72 hrs. All flow cytometry plots and graphs are representative data from at least 3 separate experiments. Red lines = unstained cells, blue lines = cells stained with CXCR3.
    Human Cxcr3 Orf Construct, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd182/CXCR3+(NM_001142797)+Human+Tagged+ORF+Clone/pmc04370421-47-5-9
    Average 90 stars, based on 3 article reviews
    human cxcr3 orf construct - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "CXCR3 Signaling in BRAF WT Melanoma Increases IL-8 Expression and Tumorigenicity"

    Article Title: CXCR3 Signaling in BRAF WT Melanoma Increases IL-8 Expression and Tumorigenicity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0121140

    (A) Flow cytometry analysis of CXCR3 protein expression on a VGP melanoma cell line, C9. Cells were incubated with serum at 5% CO 2 . (B) Graph of CXCR3 protein expression on BOWES cells from 8 separate flow cytometry experiments. Cells were cultured either in normal culture conditions (far left bar), in serum-free media at 8% CO 2 for 24, 48, or 72 hrs, or in serum-free media at 8% CO 2 for 48 hrs followed by 48 hrs in normal culture conditions (far right bar). T-tests were performed on the averages for each time point, comparing treatment cells with the 0hr time point (NS = P>0.05, *** = P≤ 0.001). (C-D) Flow cytometry analysis of CXCR3 protein expression on an RGP cell line, BOWES. Cells were incubated (C) with serum at 5% CO 2 or (D) without serum at 8% CO 2 for 72 hrs. All flow cytometry plots and graphs are representative data from at least 3 separate experiments. Red lines = unstained cells, blue lines = cells stained with CXCR3.
    Figure Legend Snippet: (A) Flow cytometry analysis of CXCR3 protein expression on a VGP melanoma cell line, C9. Cells were incubated with serum at 5% CO 2 . (B) Graph of CXCR3 protein expression on BOWES cells from 8 separate flow cytometry experiments. Cells were cultured either in normal culture conditions (far left bar), in serum-free media at 8% CO 2 for 24, 48, or 72 hrs, or in serum-free media at 8% CO 2 for 48 hrs followed by 48 hrs in normal culture conditions (far right bar). T-tests were performed on the averages for each time point, comparing treatment cells with the 0hr time point (NS = P>0.05, *** = P≤ 0.001). (C-D) Flow cytometry analysis of CXCR3 protein expression on an RGP cell line, BOWES. Cells were incubated (C) with serum at 5% CO 2 or (D) without serum at 8% CO 2 for 72 hrs. All flow cytometry plots and graphs are representative data from at least 3 separate experiments. Red lines = unstained cells, blue lines = cells stained with CXCR3.

    Techniques Used: Flow Cytometry, Expressing, Incubation, Cell Culture, Staining

    Gene expression of stressed BOWES cells (cultured in serum-free media at 8% CO 2 ) relative to those cultured under normal serum-containing conditions (10% FBS media at 5% CO 2 ).
    Figure Legend Snippet: Gene expression of stressed BOWES cells (cultured in serum-free media at 8% CO 2 ) relative to those cultured under normal serum-containing conditions (10% FBS media at 5% CO 2 ).

    Techniques Used: Gene Expression, Cell Culture

    (A) BOWES cells were cultured for 48 hrs under stressful conditions (serum-free media at 8% CO 2 ) in the presence of ligand (100ng/ml CXCL9 and CXCL10), with the addition of DMSO, 0.2μM, or 1μM AMG487. IL-8 expression was measured with RT-PCR, fold change was calculated relative to cells treated 1μM AMG487. (B) Flow cytometry analysis of representative data from 3 separate sorts. CXCR3 Low and CXCR3 High cells were sorted based on the gates shown. Red line = unstained cells, blue line = cells stained with CXCR3. (C) CXCR3 and (D) IL-8 RT-PCR results from unsorted cells grown with serum in 5% CO 2 or without serum in 8% CO 2 (stressed), and sorted stressed cells (CXCR3 Low and CXCR3 High ). Fold change was calculated relative to unsorted stressed cells. T-tests were performed on the relative fold changes, pooled data from 3 separate experiments (* = P≤ 0.05, ** = P≤0.01, ***P≤ 0.001).
    Figure Legend Snippet: (A) BOWES cells were cultured for 48 hrs under stressful conditions (serum-free media at 8% CO 2 ) in the presence of ligand (100ng/ml CXCL9 and CXCL10), with the addition of DMSO, 0.2μM, or 1μM AMG487. IL-8 expression was measured with RT-PCR, fold change was calculated relative to cells treated 1μM AMG487. (B) Flow cytometry analysis of representative data from 3 separate sorts. CXCR3 Low and CXCR3 High cells were sorted based on the gates shown. Red line = unstained cells, blue line = cells stained with CXCR3. (C) CXCR3 and (D) IL-8 RT-PCR results from unsorted cells grown with serum in 5% CO 2 or without serum in 8% CO 2 (stressed), and sorted stressed cells (CXCR3 Low and CXCR3 High ). Fold change was calculated relative to unsorted stressed cells. T-tests were performed on the relative fold changes, pooled data from 3 separate experiments (* = P≤ 0.05, ** = P≤0.01, ***P≤ 0.001).

    Techniques Used: Cell Culture, Expressing, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, Staining

    BOWES cells stably transfected with CXCR3 or an empty vector (PCMV6) were grown in serum-containing media. (A) Flow cytometry and (B) RT-PCR were used to measure CXCR3 expression. (A) BOWES WT (red line), BOWES PCMV6 clone #1(blue line), and BOWES CXCR3 clones #1 (orange line), #2 (light green), and #3 (dark green line). (B) RT-PCR was normalized to CXCR3 clone #2. (C) BOWES PCMV6 and CXCR3 cells were grown in serum-free media for 2 hrs, then in the presence of CXCL9 and CXCL10 for 2, 5, 10, 20, or 30 minutes. Total protein was isolated and probed for pERK and total ERK via immunoblot analysis. (D) BOWES PCMV6 and CXCR3 cells were plated on a membrane above serum-free media with CXCL9/10 (+ligand) or without (-ligand), and total migrated cells per field (40X objective) were quantified after 6hrs. Cells were also plated on the membrane in the presence or absence of AMG487. T-tests were performed on the BOWES CXCR3 migrated cells ± ligand in the presence and absence of AMG487 (NS = P>0.05, **** = P≤ 0.0001). (E) BOWES PCMV6 and CXCR3 cells were grown in serum-containing media for 48 hrs in the presence of ligand, with 0, 0.2, or 1 μM AMG487. Expression of IL-8 was measured via RT-PCR, fold change was calculated relative to BOWES PCMV6 0 μM. T-tests were performed on the relative fold changes, representative data from 3 separate experiments (**** = P≤ 0.0001).
    Figure Legend Snippet: BOWES cells stably transfected with CXCR3 or an empty vector (PCMV6) were grown in serum-containing media. (A) Flow cytometry and (B) RT-PCR were used to measure CXCR3 expression. (A) BOWES WT (red line), BOWES PCMV6 clone #1(blue line), and BOWES CXCR3 clones #1 (orange line), #2 (light green), and #3 (dark green line). (B) RT-PCR was normalized to CXCR3 clone #2. (C) BOWES PCMV6 and CXCR3 cells were grown in serum-free media for 2 hrs, then in the presence of CXCL9 and CXCL10 for 2, 5, 10, 20, or 30 minutes. Total protein was isolated and probed for pERK and total ERK via immunoblot analysis. (D) BOWES PCMV6 and CXCR3 cells were plated on a membrane above serum-free media with CXCL9/10 (+ligand) or without (-ligand), and total migrated cells per field (40X objective) were quantified after 6hrs. Cells were also plated on the membrane in the presence or absence of AMG487. T-tests were performed on the BOWES CXCR3 migrated cells ± ligand in the presence and absence of AMG487 (NS = P>0.05, **** = P≤ 0.0001). (E) BOWES PCMV6 and CXCR3 cells were grown in serum-containing media for 48 hrs in the presence of ligand, with 0, 0.2, or 1 μM AMG487. Expression of IL-8 was measured via RT-PCR, fold change was calculated relative to BOWES PCMV6 0 μM. T-tests were performed on the relative fold changes, representative data from 3 separate experiments (**** = P≤ 0.0001).

    Techniques Used: Stable Transfection, Transfection, Plasmid Preparation, Flow Cytometry, Reverse Transcription Polymerase Chain Reaction, Expressing, Clone Assay, Isolation, Western Blot, Membrane

    WM35 IL-8 signaling is CXCR3-signaling independent. (A) BOWES WT cells were grown in serum-free media for 48 hrs in the presence of ligand, with 0, 0.2, or 1 μM AMG487 and with DMSO (black bars) or 3 μM U0126 (gray bars). Expression of IL-8 was measured via RT-PCR, fold change was calculated relative to cells treated with 0 μM U0126 and 1 μM AMG487. (B-C) Flow cytometry analysis of CXCR3 protein expression on the BRAF V600E RGP cell line, WM35. Cells were incubated (B) with 5% serum at 5% CO 2 or (C) without serum at 8% CO 2 for 72 hrs. Red lines = unstained cells, blue lines = cells stained with CXCR3. (D) BOWES WT (black bars) and WM35 cells (gray bars) were grown in serum-free media for 48 hrs in the presence of ligand, with 0, 0.2, or 1 μM AMG487. Expression of IL-8 was measured via RT-PCR, fold change was calculated relative to BOWES cells treated with 0 μM AMG487. (E) WM35 cells were grown in serum-free media for 48 hrs in the presence of ligand, with 0, 0.2, or 1 μM AMG487 and either DMSO (black bars), 3 μM U0126 (striped bars), or 3 μM PLX4032 (gray bars). Expression of IL-8 was measured via RT-PCR, fold change was calculated relative to WM35 cells treated with 0 μM AMG487 and 3 μM U0126. All T-tests were performed on the relative fold changes, all data is representative from at least 3 separate experiments (NS = P>0.5, * = P≤ 0.05, ** = P≤0.01, **** = P≤ 0.0001).
    Figure Legend Snippet: WM35 IL-8 signaling is CXCR3-signaling independent. (A) BOWES WT cells were grown in serum-free media for 48 hrs in the presence of ligand, with 0, 0.2, or 1 μM AMG487 and with DMSO (black bars) or 3 μM U0126 (gray bars). Expression of IL-8 was measured via RT-PCR, fold change was calculated relative to cells treated with 0 μM U0126 and 1 μM AMG487. (B-C) Flow cytometry analysis of CXCR3 protein expression on the BRAF V600E RGP cell line, WM35. Cells were incubated (B) with 5% serum at 5% CO 2 or (C) without serum at 8% CO 2 for 72 hrs. Red lines = unstained cells, blue lines = cells stained with CXCR3. (D) BOWES WT (black bars) and WM35 cells (gray bars) were grown in serum-free media for 48 hrs in the presence of ligand, with 0, 0.2, or 1 μM AMG487. Expression of IL-8 was measured via RT-PCR, fold change was calculated relative to BOWES cells treated with 0 μM AMG487. (E) WM35 cells were grown in serum-free media for 48 hrs in the presence of ligand, with 0, 0.2, or 1 μM AMG487 and either DMSO (black bars), 3 μM U0126 (striped bars), or 3 μM PLX4032 (gray bars). Expression of IL-8 was measured via RT-PCR, fold change was calculated relative to WM35 cells treated with 0 μM AMG487 and 3 μM U0126. All T-tests were performed on the relative fold changes, all data is representative from at least 3 separate experiments (NS = P>0.5, * = P≤ 0.05, ** = P≤0.01, **** = P≤ 0.0001).

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, Incubation, Staining

    Tumor growth in vivo .
    Figure Legend Snippet: Tumor growth in vivo .

    Techniques Used: In Vivo, Injection

    BOWES PCMV6 and CXCR3 cells were injected interdermally into NSG mice (5 x 10 5 cells per injection). Tumors were measured weekly with calipers and tumor volume (mm 3 ) was calculated. Mean tumor volume and SEM were calculated from mice that had measurable tumors. (A) The graph shows mean tumor volume (with SEM) over the 6 week period. Linear regression analysis was performed on the two slopes (P = 0.006). (B) Tumor tissue from injected mice was resected at the time of harvest and RNA was isolated. IL-8 expression was measured in BOWES PCMV6 and CXCR3 tumor tissue via RT-PCR (**** = P≤ 0.0001). Representative individual tumors are presented in graph.
    Figure Legend Snippet: BOWES PCMV6 and CXCR3 cells were injected interdermally into NSG mice (5 x 10 5 cells per injection). Tumors were measured weekly with calipers and tumor volume (mm 3 ) was calculated. Mean tumor volume and SEM were calculated from mice that had measurable tumors. (A) The graph shows mean tumor volume (with SEM) over the 6 week period. Linear regression analysis was performed on the two slopes (P = 0.006). (B) Tumor tissue from injected mice was resected at the time of harvest and RNA was isolated. IL-8 expression was measured in BOWES PCMV6 and CXCR3 tumor tissue via RT-PCR (**** = P≤ 0.0001). Representative individual tumors are presented in graph.

    Techniques Used: Injection, Isolation, Expressing, Reverse Transcription Polymerase Chain Reaction

    Related Articles

    Transfection:

    Article Title: C-X-C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C-X-C motif ligand 10
    Article Snippet: .. After 16 h, the cells were transfected with 500 ng CXCR3 (Myc-DDK-tagged) transcript variant A plasmid DNA or CXCR3 (Myc-DDK-tagged) transcript variant B plasmid DNA (both from Origene Technologies) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) per manufacturer's protocol. ..

    Article Title: CXCR3 Signaling in BRAF WT Melanoma Increases IL-8 Expression and Tumorigenicity
    Article Snippet: .. Cells were transfected with a human CXCR3 ORF construct (Origene, Rockville, MD, USA) (under control of the CMV promoter, with Neomycin resistance) or a pCMV6 empty vector control (Origene), using Lipofectamine 3000 (Life Technologies), according to the manufacturer’s instructions. .. Transfected cells were grown with 500μg/ml G418 (Corning) and plated at a clonal density to yield stable clones.

    Variant Assay:

    Article Title: C-X-C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C-X-C motif ligand 10
    Article Snippet: .. After 16 h, the cells were transfected with 500 ng CXCR3 (Myc-DDK-tagged) transcript variant A plasmid DNA or CXCR3 (Myc-DDK-tagged) transcript variant B plasmid DNA (both from Origene Technologies) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) per manufacturer's protocol. ..

    Plasmid Preparation:

    Article Title: C-X-C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C-X-C motif ligand 10
    Article Snippet: .. After 16 h, the cells were transfected with 500 ng CXCR3 (Myc-DDK-tagged) transcript variant A plasmid DNA or CXCR3 (Myc-DDK-tagged) transcript variant B plasmid DNA (both from Origene Technologies) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) per manufacturer's protocol. ..

    Article Title: CXCR3 Signaling in BRAF WT Melanoma Increases IL-8 Expression and Tumorigenicity
    Article Snippet: .. Cells were transfected with a human CXCR3 ORF construct (Origene, Rockville, MD, USA) (under control of the CMV promoter, with Neomycin resistance) or a pCMV6 empty vector control (Origene), using Lipofectamine 3000 (Life Technologies), according to the manufacturer’s instructions. .. Transfected cells were grown with 500μg/ml G418 (Corning) and plated at a clonal density to yield stable clones.

    Construct:

    Article Title: CXCR3 Signaling in BRAF WT Melanoma Increases IL-8 Expression and Tumorigenicity
    Article Snippet: .. Cells were transfected with a human CXCR3 ORF construct (Origene, Rockville, MD, USA) (under control of the CMV promoter, with Neomycin resistance) or a pCMV6 empty vector control (Origene), using Lipofectamine 3000 (Life Technologies), according to the manufacturer’s instructions. .. Transfected cells were grown with 500μg/ml G418 (Corning) and plated at a clonal density to yield stable clones.

    Control:

    Article Title: CXCR3 Signaling in BRAF WT Melanoma Increases IL-8 Expression and Tumorigenicity
    Article Snippet: .. Cells were transfected with a human CXCR3 ORF construct (Origene, Rockville, MD, USA) (under control of the CMV promoter, with Neomycin resistance) or a pCMV6 empty vector control (Origene), using Lipofectamine 3000 (Life Technologies), according to the manufacturer’s instructions. .. Transfected cells were grown with 500μg/ml G418 (Corning) and plated at a clonal density to yield stable clones.



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    Image Search Results


    Identification of the key genes expression in external datasets. A IFIT5, IFI16, TRIM22, IFI44L, IRF9, OAS3, BST2, CASP1, CXCR2 were statistically significant in AF expression profile ( GSE41177 ). B IFIT5、CASP1 and CXCR2 were statistically significant in COPD expression profile ( GSE56766 ). C - D ROC of IFIT5、CASP1 and CXCR2 in the AF( GSE79768 ) dataset. -, not significant.;*, p <0.05; **, p <0.01; ***, p < 0.001

    Journal: BMC Medical Genomics

    Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

    doi: 10.1186/s12920-026-02335-4

    Figure Lengend Snippet: Identification of the key genes expression in external datasets. A IFIT5, IFI16, TRIM22, IFI44L, IRF9, OAS3, BST2, CASP1, CXCR2 were statistically significant in AF expression profile ( GSE41177 ). B IFIT5、CASP1 and CXCR2 were statistically significant in COPD expression profile ( GSE56766 ). C - D ROC of IFIT5、CASP1 and CXCR2 in the AF( GSE79768 ) dataset. -, not significant.;*, p <0.05; **, p <0.01; ***, p < 0.001

    Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

    Techniques: Expressing

    Validation of CASP1, CXCR2, and IFIT5 expression in different clinical groups at mRNA and protein levels. A – C Relative mRNA expression levels of CASP1, IFIT5, and CXCR2 were assessed by qRT-PCR in peripheral blood samples from healthy controls (CON), atrial fibrillation (AF), chronic obstructive pulmonary disease (COPD), and AF combined with COPD (AF+COPD). D – F Quantification of CASP1, IFIT5, and CXCR2 protein expression levels based on Western blot analysis. Signal intensities were normalized to GAPDH. G – I Serum protein concentrations of CASP1 (pmol/L), IFIT5 (pg/mL), and CXCR2 (ng/mL) measured by ELISA in the four groups. J Representative Western blot images for CASP1, IFIT5, and CXCR2 with GAPDH as loading control. *, p < 0.05; **, p <0.01; ***, p < 0.001

    Journal: BMC Medical Genomics

    Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

    doi: 10.1186/s12920-026-02335-4

    Figure Lengend Snippet: Validation of CASP1, CXCR2, and IFIT5 expression in different clinical groups at mRNA and protein levels. A – C Relative mRNA expression levels of CASP1, IFIT5, and CXCR2 were assessed by qRT-PCR in peripheral blood samples from healthy controls (CON), atrial fibrillation (AF), chronic obstructive pulmonary disease (COPD), and AF combined with COPD (AF+COPD). D – F Quantification of CASP1, IFIT5, and CXCR2 protein expression levels based on Western blot analysis. Signal intensities were normalized to GAPDH. G – I Serum protein concentrations of CASP1 (pmol/L), IFIT5 (pg/mL), and CXCR2 (ng/mL) measured by ELISA in the four groups. J Representative Western blot images for CASP1, IFIT5, and CXCR2 with GAPDH as loading control. *, p < 0.05; **, p <0.01; ***, p < 0.001

    Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

    Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Control

    A Transcription factor (TF)-gene interaction network based on key genes. B miRNA-gene interaction network based on key genes. C , E , G Overall docking models of CASP1, CXCR2, and IFIT5 proteins with estradiol, along with two-dimensional and three-dimensional spatial interaction diagrams of the ligand-protein complexes. D , F , H Overall docking models of CASP1, CXCR2, and IFIT5 proteins with methyl methanesulfonate, along with two-dimensional and three-dimensional spatial interaction diagrams of the ligand-protein complexes

    Journal: BMC Medical Genomics

    Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

    doi: 10.1186/s12920-026-02335-4

    Figure Lengend Snippet: A Transcription factor (TF)-gene interaction network based on key genes. B miRNA-gene interaction network based on key genes. C , E , G Overall docking models of CASP1, CXCR2, and IFIT5 proteins with estradiol, along with two-dimensional and three-dimensional spatial interaction diagrams of the ligand-protein complexes. D , F , H Overall docking models of CASP1, CXCR2, and IFIT5 proteins with methyl methanesulfonate, along with two-dimensional and three-dimensional spatial interaction diagrams of the ligand-protein complexes

    Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

    Techniques: