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anti resource source identifier cd14 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti resource source identifier cd14 microbeads
    Anti Resource Source Identifier Cd14 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 4731 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14/CD14+MicroBeads%2C+human/pm42085186-52-2-9
    Average 98 stars, based on 4731 article reviews
    anti resource source identifier cd14 microbeads - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Isolation:

    Article Title: A Selective Pro‐Oxidant Drives Redox Reprogramming to Induce Ferroptosis and Immune Remodeling in Glioblastoma
    Article Snippet: .. CD14 + monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using Ficoll‐Paque (GE Healthcare, Piscataway, NJ, USA) density‐gradient centrifugation followed by magnetic bead selection with CD14 MicroBeads (Miltenyi Biotec, Cologne, Germany) according to the manufacturer's protocol. ..

    Article Title: CTLA4 rs3087243-A-linked haplotype is associated with increased CTLA-4 expression and suppressive function in human regulatory T cells.
    Article Snippet: .. CD14+ monocytes were isolated from PBMCs of a healthy adult donor using CD14 MicroBeads (Miltenyi Biotec, #130-050-201). .. To generate monocyte-derived immature dendritic cells (iDCs), 1 × 106 CD14+ monocytes were plated in 2 mL RPMI with 10% fetal bovine serum [RPMI-FBS]) and 100 ng/mL of GM-CSF (Miltenyi Biotec, #130-095-372) and IL-4 (Miltenyi Biotec, #130-093-917) on a 12-well, flat-bottom tissue culture plate.

    Article Title: Metabolically encoded cell-surface glycoRNA remodeling enhances antitumor immunity
    Article Snippet: Peripheral blood mononuclear cells were isolated from buffy coats by Ficoll-Paque density gradient centrifugation. .. CD14+ monocytes were isolated by positive selection using human CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201) according to the manufacturer’s instructions and cultured in RPMI-1640 containing 10% fetal bovine serum, 20 ng/mL recombinant human GM-CSF and 10 ng/mL recombinant human IL-4, with half-medium changes on days 3 and 5. .. Day-7 moDCs were transfected with the indicated RNA preparations (native, PNGase F-treated, RNase cocktail-treated, or PNGase F plus RNase cocktail-treated, prepared as described above; 500 ng per well in 24-well plates) using Lipofectamine 3000 for 24 h; untreated moDCs and LPS (100 ng/mL, Sigma-Aldrich, Cat# L2630)-treated moDCs served as negative and positive controls.

    Article Title: Anti-TNFR2 antibody BI-1808 induces tumor regression and antitumor T cell immunity through T reg and myeloid cell modulation in patients with solid tumors
    Article Snippet: .. CD14+ monocytes were isolated from 20x106 PBMCs using an -human 394 CD14 microbeads ( Miltenyi Cat# 130-050-201, RRID:AB_2665482) according to the manufacturer’s 395 protocol. .. 2x105 isolated monocytes were cultured per well with 10 μg/mL of TNFR2 mAb or isotype 396 control in RPMI 1640+Glutamax (Invitrogen Cat# 61870-610) and 10 % HI FBS (Invitrogen Cat# 26140-397 079) for 20 h at 37 oC and 5 % CO2.

    Centrifugation:

    Article Title: A Selective Pro‐Oxidant Drives Redox Reprogramming to Induce Ferroptosis and Immune Remodeling in Glioblastoma
    Article Snippet: .. CD14 + monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using Ficoll‐Paque (GE Healthcare, Piscataway, NJ, USA) density‐gradient centrifugation followed by magnetic bead selection with CD14 MicroBeads (Miltenyi Biotec, Cologne, Germany) according to the manufacturer's protocol. ..

    Selection:

    Article Title: A Selective Pro‐Oxidant Drives Redox Reprogramming to Induce Ferroptosis and Immune Remodeling in Glioblastoma
    Article Snippet: .. CD14 + monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using Ficoll‐Paque (GE Healthcare, Piscataway, NJ, USA) density‐gradient centrifugation followed by magnetic bead selection with CD14 MicroBeads (Miltenyi Biotec, Cologne, Germany) according to the manufacturer's protocol. ..

    Article Title: Metabolically encoded cell-surface glycoRNA remodeling enhances antitumor immunity
    Article Snippet: Peripheral blood mononuclear cells were isolated from buffy coats by Ficoll-Paque density gradient centrifugation. .. CD14+ monocytes were isolated by positive selection using human CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201) according to the manufacturer’s instructions and cultured in RPMI-1640 containing 10% fetal bovine serum, 20 ng/mL recombinant human GM-CSF and 10 ng/mL recombinant human IL-4, with half-medium changes on days 3 and 5. .. Day-7 moDCs were transfected with the indicated RNA preparations (native, PNGase F-treated, RNase cocktail-treated, or PNGase F plus RNase cocktail-treated, prepared as described above; 500 ng per well in 24-well plates) using Lipofectamine 3000 for 24 h; untreated moDCs and LPS (100 ng/mL, Sigma-Aldrich, Cat# L2630)-treated moDCs served as negative and positive controls.

    Purification:

    Article Title: Mitochondria transfer via tunneling nanotubes drives macrophage immunosuppression and metabolic reprogramming in pediatric AML.
    Article Snippet: Human monocyte-derived macrophages generation and polarization Peripheral blood mononuclear cells (PBMC) from healthy donors (Sanquin, Amsterdam, The Netherlands) were isolated by a densitygradient centrifugation using Lymphoprep (StemCell, Cologne, Germany). .. CD14+ monocytes were purified by MACS using CD14 MicroBeads (Miltenyi Biotec, Leiden, The Netherlands). .. Monocytes were differentiated into macrophages for 5 days in RPMI-1640 supplemented with 10% heat-inactivated human serum (SigmaAldrich, Zwijndrecht, The Netherlands) and 20 ng/mL M-CSF (PeproTech ThermoFisher, Breda, The Netherlands).

    Magnetic Cell Separation:

    Article Title: Mitochondria transfer via tunneling nanotubes drives macrophage immunosuppression and metabolic reprogramming in pediatric AML.
    Article Snippet: Human monocyte-derived macrophages generation and polarization Peripheral blood mononuclear cells (PBMC) from healthy donors (Sanquin, Amsterdam, The Netherlands) were isolated by a densitygradient centrifugation using Lymphoprep (StemCell, Cologne, Germany). .. CD14+ monocytes were purified by MACS using CD14 MicroBeads (Miltenyi Biotec, Leiden, The Netherlands). .. Monocytes were differentiated into macrophages for 5 days in RPMI-1640 supplemented with 10% heat-inactivated human serum (SigmaAldrich, Zwijndrecht, The Netherlands) and 20 ng/mL M-CSF (PeproTech ThermoFisher, Breda, The Netherlands).

    Cell Culture:

    Article Title: Metabolically encoded cell-surface glycoRNA remodeling enhances antitumor immunity
    Article Snippet: Peripheral blood mononuclear cells were isolated from buffy coats by Ficoll-Paque density gradient centrifugation. .. CD14+ monocytes were isolated by positive selection using human CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201) according to the manufacturer’s instructions and cultured in RPMI-1640 containing 10% fetal bovine serum, 20 ng/mL recombinant human GM-CSF and 10 ng/mL recombinant human IL-4, with half-medium changes on days 3 and 5. .. Day-7 moDCs were transfected with the indicated RNA preparations (native, PNGase F-treated, RNase cocktail-treated, or PNGase F plus RNase cocktail-treated, prepared as described above; 500 ng per well in 24-well plates) using Lipofectamine 3000 for 24 h; untreated moDCs and LPS (100 ng/mL, Sigma-Aldrich, Cat# L2630)-treated moDCs served as negative and positive controls.

    Recombinant:

    Article Title: Metabolically encoded cell-surface glycoRNA remodeling enhances antitumor immunity
    Article Snippet: Peripheral blood mononuclear cells were isolated from buffy coats by Ficoll-Paque density gradient centrifugation. .. CD14+ monocytes were isolated by positive selection using human CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201) according to the manufacturer’s instructions and cultured in RPMI-1640 containing 10% fetal bovine serum, 20 ng/mL recombinant human GM-CSF and 10 ng/mL recombinant human IL-4, with half-medium changes on days 3 and 5. .. Day-7 moDCs were transfected with the indicated RNA preparations (native, PNGase F-treated, RNase cocktail-treated, or PNGase F plus RNase cocktail-treated, prepared as described above; 500 ng per well in 24-well plates) using Lipofectamine 3000 for 24 h; untreated moDCs and LPS (100 ng/mL, Sigma-Aldrich, Cat# L2630)-treated moDCs served as negative and positive controls.



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