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cd137 biotin antibodies miltenyi  (Miltenyi Biotec)


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    Miltenyi Biotec cd137 biotin antibodies miltenyi
    Cd137 Biotin Antibodies Miltenyi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd137+microbead+kit/CD137+MicroBead+Kit%2C+human/pm40359936-252-147-150
    Average 94 stars, based on 34 article reviews
    cd137 biotin antibodies miltenyi - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Phenotypes of synovial fluid Treg cells in checkpoint blockade-related inflammatory arthritis
    Article Snippet: After three washes with cold BD sample buffer, cells were resuspended at 20,000–30,000 cells/mL and loaded onto BD Rhapsody cartridges (#633733; BD Biosciences, Franklin Lake, New Jersey, USA) for single-cell capture. .. An immunoactivating Treg subset was isolated using CD137 microbeads (Miltenyi Biotec, Cologne, Germany, #130-093-476) through a 15-min 4°C incubation in PBS/0.5% BSA/2 mM EDTA buffer (cell density ≤ 10 8 /mL; 10 μL beads per 10 7 cells), followed by column-based separation where microbead − cells were collected in flow-through, and microbead + cells were eluted after column removal. .. Similarly, immunoactivating Treg subsets from PBMC were sorted using the same microbeads by positive selection.

    Article Title: Discover personalized drug opportunity and optimized tumor-infiltrating lymphocyte therapy for rare cancer: an organoid-based whole-journey clinical mapping study in a neuroendocrine cancer patient.
    Article Snippet: .. On day 2, PTO-specific CD137 expression was confirmed via flow cytometric analysis, and CD137+ cells were isolated using a CD137 MicroBead kit according to the manufacturer’s instructions (Miltenyi). .. Sorted CD137+/− TILs were rested and expanded in expansion medium (X-VIVO15 supplemented with 2 mM glutamine, IL-2 (1000 U/mL) (ACROBiosystems), IL-15 (10 ng/mL) (ACROBiosystems), IL-21 (10 ng/mL) (ACROBiosystems), 1:100 penicillin/streptomycin, and 10% male human AB serum (Sigma-Aldrich) for 7–14 days at 37 °C with 5% CO2 until functional assessment.

    Article Title: Discover personalized drug opportunity and optimized tumor-infiltrating lymphocyte therapy for rare cancer: an organoid-based whole-journey clinical mapping study in a neuroendocrine cancer patient
    Article Snippet: .. On day 2, PTO-specific CD137 expression was confirmed via flow cytometric analysis, and CD137 + cells were isolated using a CD137 MicroBead kit according to the manufacturer’s instructions (Miltenyi). .. Sorted CD137 +/− TILs were rested and expanded in expansion medium (X-VIVO15 supplemented with 2 mM glutamine, IL-2 (1000 U/mL) (ACROBiosystems), IL-15 (10 ng/mL) (ACROBiosystems), IL-21 (10 ng/mL) (ACROBiosystems), 1:100 penicillin/streptomycin, and 10% male human AB serum (Sigma-Aldrich) for 7–14 days at 37 °C with 5% CO 2 until functional assessment.

    Incubation:

    Article Title: Phenotypes of synovial fluid Treg cells in checkpoint blockade-related inflammatory arthritis
    Article Snippet: After three washes with cold BD sample buffer, cells were resuspended at 20,000–30,000 cells/mL and loaded onto BD Rhapsody cartridges (#633733; BD Biosciences, Franklin Lake, New Jersey, USA) for single-cell capture. .. An immunoactivating Treg subset was isolated using CD137 microbeads (Miltenyi Biotec, Cologne, Germany, #130-093-476) through a 15-min 4°C incubation in PBS/0.5% BSA/2 mM EDTA buffer (cell density ≤ 10 8 /mL; 10 μL beads per 10 7 cells), followed by column-based separation where microbead − cells were collected in flow-through, and microbead + cells were eluted after column removal. .. Similarly, immunoactivating Treg subsets from PBMC were sorted using the same microbeads by positive selection.

    other:

    Article Title: Generation of T cell responses against broad KRAS hotspot neoantigens for cell therapy or TCR discovery.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PGE-1 Pfizer Cat#0009-3169-06 Synthetic Peptides (Figure S1) BioNTech (in-house) N/A GMCSF CellGenix Cat#1412-050 Anti CD3/CD28 Beads ThermoFisher Cat#11132D Human CD3/CD28 T cell Activator STEMCELL Technologies Cat#10971 MACS GMP CD28 pure Miltenyi Cat#170-076-117 MACS GMP T cell TransAct Large Scale Miltenyi Cat#200-076-204 PLASMA-LYTE A Fisher Scientific Cat#NC2087616 CryoStor CS10 BioLife Cat#210502 DMSO Miltenyi Cat#170-076-303 AIM-V Medium ThermoFisher Cat#12055083 Fetal Bovine Serum (Heat Inactivated) Corning Cat#35-011-CV Human Serum Albumin Shire Cat#0944-0493-02 Human Serum Sigma Cat#H4522 CTS Immune Cell SR ThermoFisher Cat#A2596101 HyClone Cell Boost 6 Supplement Cytiva Cat#SH31118.03 IncuCyte Annexin V red reagent Sartorius Cat#4641 Pulmozyme (DNAse) Genentech Cat#50242-100-40 Puromycin InvivoGen Cat#ant-pr-1 FuGENE Transfection Reagent Promega Cat#E2311 Lenti-X Concentrator Takara Cat#631231 Precision Count Beads BioLegend Cat#424902 Critical commercial assays Chromium Next GEM Automated Single Cell 5′ cDNA Kit v2 10× genomics Cat#1000425 Chromium Single Cell Human TCR Amplification Kit 10× genomics Cat#1000252 Chromium Next GEM Chip K Automated Single Cell Kit 10× genomics Cat#1000289 Chromium i7 Dual Index Kit TT 10× genomics Cat#1000215 Chromium i7 Dual Index Kit TN 10× genomics Cat#1000242 PhiX Control Illumina Cat#FC-110-3001 MiSeq Reagent Kit v3 (600-cycles) Illumina Cat#MS-102-3003 Simple Plex Human IFN-γ third Gen Bio-Techne Cat#SPCKB-PS-002574 Lenti-X GoStix Plus Takara Cat#631280 Deposited data TCR Sequences This paper GenBank (PV444443, PV444444, PV444445, PV444446, PV444447, PV444448, PV444449, PV444450, PV444451, PV444452, PV444453, PV444454) Experimental models: Cell lines 293T cell line ATCC Cat#CRL-3216; RRID: CVCL_0063 293Vec-GalV cell line BIONTECH IMFS N/A A375 cell line ATCC Cat#CRL-1619; RRID: CVCL_0132 AGS cell line ATCC Cat#CRL-1739; RRID: CVCL_0139 E6.1 Jurkat cells ATCC Cat#TIB-152; RRID: CVCL_0367 J.RT3-T3.5 Jurkat cells ATCC Cat#TIB-153; RRID: CVCL_1316 NCI-H727 cell line ATCC Cat#CRL-5815; RRID: CVCL_1584 SW620 cell line ATCC Cat#CCL-227; RRID: CVCL_0547 Experimental models: Organisms/strains NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl Charles River Laboratories NCG; RRID: IMSR_CRL:572 Recombinant DNA pCDH-SFFV-Puro-GFP Lenti vector In-house N/A (Continued on next page) Cell Reports Methods 5, 101049, May 19, 2025 e2 Article ll OPEN ACCESS

    Expressing:

    Article Title: Enhancement of immune checkpoint inhibitor efficacy by DHX58+ SELL+ T cells in advanced gastric cancer
    Article Snippet: .. For expansion of tumor-reactive sublines, T cells were sorted on the basis of CD137 expression 24 h after stimulation with tumor organoids, using a CD137 Microbead Kit (Miltenyi) followingmanufacturer’s instructions and expanded as described above. ..

    Article Title: Discover personalized drug opportunity and optimized tumor-infiltrating lymphocyte therapy for rare cancer: an organoid-based whole-journey clinical mapping study in a neuroendocrine cancer patient.
    Article Snippet: .. On day 2, PTO-specific CD137 expression was confirmed via flow cytometric analysis, and CD137+ cells were isolated using a CD137 MicroBead kit according to the manufacturer’s instructions (Miltenyi). .. Sorted CD137+/− TILs were rested and expanded in expansion medium (X-VIVO15 supplemented with 2 mM glutamine, IL-2 (1000 U/mL) (ACROBiosystems), IL-15 (10 ng/mL) (ACROBiosystems), IL-21 (10 ng/mL) (ACROBiosystems), 1:100 penicillin/streptomycin, and 10% male human AB serum (Sigma-Aldrich) for 7–14 days at 37 °C with 5% CO2 until functional assessment.

    Article Title: Discover personalized drug opportunity and optimized tumor-infiltrating lymphocyte therapy for rare cancer: an organoid-based whole-journey clinical mapping study in a neuroendocrine cancer patient
    Article Snippet: .. On day 2, PTO-specific CD137 expression was confirmed via flow cytometric analysis, and CD137 + cells were isolated using a CD137 MicroBead kit according to the manufacturer’s instructions (Miltenyi). .. Sorted CD137 +/− TILs were rested and expanded in expansion medium (X-VIVO15 supplemented with 2 mM glutamine, IL-2 (1000 U/mL) (ACROBiosystems), IL-15 (10 ng/mL) (ACROBiosystems), IL-21 (10 ng/mL) (ACROBiosystems), 1:100 penicillin/streptomycin, and 10% male human AB serum (Sigma-Aldrich) for 7–14 days at 37 °C with 5% CO 2 until functional assessment.



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    Image Search Results


    Frequencies of circulating CD4 + and CD4 + CD154 + Th cells in cohort 1 ( A ) Frequencies of CD4 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( B ) Frequencies of CD4 + CD154 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( C ) Frequencies of CD4 + CD154 + Th cells in sepsis patients after stimulation with control or indicated antigens ( n = 20). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Frequencies of circulating CD4 + and CD4 + CD154 + Th cells in cohort 1 ( A ) Frequencies of CD4 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( B ) Frequencies of CD4 + CD154 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( C ) Frequencies of CD4 + CD154 + Th cells in sepsis patients after stimulation with control or indicated antigens ( n = 20). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Control

    Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 1 ( A ) Frequencies of IFNγ producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( B ) Frequencies of TNF-α producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( C ) Frequencies of IL-2 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 1 ( A ) Frequencies of IFNγ producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( B ) Frequencies of TNF-α producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). ( C ) Frequencies of IL-2 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 20/10/20/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques:

    Frequencies of circulating CD4 + and CD4 + CD154 + Th cells in cohort 2 ( A ) Frequencies of CD4 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 10/10/10). ( B ) Frequencies of CD4 + CD154 + Th cells after stimulation with control, E. coli , C. albicans , or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Frequencies of circulating CD4 + and CD4 + CD154 + Th cells in cohort 2 ( A ) Frequencies of CD4 + Th cells after stimulation with control, E. coli , C. albicans or B. longum ( n = 10/10/10). ( B ) Frequencies of CD4 + CD154 + Th cells after stimulation with control, E. coli , C. albicans , or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Control

    Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 2. ( A ) Frequencies of IL-17 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( B ) Frequencies of IL-4 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( C ) Frequencies of IL-10 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Cytokine production in antigen-reactive CD4 + CD154 + Th cells in cohort 2. ( A ) Frequencies of IL-17 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( B ) Frequencies of IL-4 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). ( C ) Frequencies of IL-10 producing CD4 + CD154 + Th cells after stimulation with E. coli , C. albicans or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques:

    Bifidobacterium longum -reactive gut trophic Th cell signature ( A ) Frequencies of CD4 + CD154 + α4ß7 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001. ( B ) Frequencies of B. longum reactive Th cells in cohort 1 and 2. ( C ) Frequencies of IFNγ- and TNF-α producing B. longum reactive Th cells in cohort 1 and 2. ( D ) Frequencies of gut trophic α4ß7 + -expressing B. longum- reactive Th cells. (B) and (C) Merged data from both cohorts. Color code indicating respective groups

    Journal: Gut Pathogens

    Article Title: “ Bifidobacterium longum -reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis”

    doi: 10.1186/s13099-025-00770-9

    Figure Lengend Snippet: Bifidobacterium longum -reactive gut trophic Th cell signature ( A ) Frequencies of CD4 + CD154 + α4ß7 + Th cells after stimulation with E. coli , C. albicans , or B. longum ( n = 10/10/10). Data represented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001. ( B ) Frequencies of B. longum reactive Th cells in cohort 1 and 2. ( C ) Frequencies of IFNγ- and TNF-α producing B. longum reactive Th cells in cohort 1 and 2. ( D ) Frequencies of gut trophic α4ß7 + -expressing B. longum- reactive Th cells. (B) and (C) Merged data from both cohorts. Color code indicating respective groups

    Article Snippet: Subsequently, cells were labeled with anti-CD154 (both cohorts) and anti-CD137 (only cohort 1) biotin antibodies followed by anti-biotin MicroBeads (CD154 & CD137 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany) and magnetically enriched with MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Expressing