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miltenyi biotec cd133 microbead  (Miltenyi Biotec)


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    Miltenyi Biotec miltenyi biotec cd133 microbead
    Miltenyi Biotec Cd133 Microbead, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd133+microbead+kit/CD133+MicroBead+Kit%2C+human+-+lyophilized/pm41870841-50-5-5
    Average 95 stars, based on 112 article reviews
    miltenyi biotec cd133 microbead - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: A novel chalcone analog inhibits cancer stemness in CD44 + and CD133 + populations via suppression of ERK signaling.
    Article Snippet: The spheroid culture medium consisted of Advanced DMEM/F12, supplemented with B27 (1×; Cat. No. 17504044, Gibco, Thermo Fisher Scientific, Waltham, MA, USA), N2 (1×, Cat. No. 17502048, Gibco), epidermal growth factor (EGF, 10 ng/ mL; Cat. No. 17502048, Gibco), and basic fibroblast growth factor (bFGF, 10 ng/mL; Cat. No. GF003AF, Sigma-Aldrich, St. Louis, MO, USA). .. CD44+ or CD133+ spheroid populations were isolated from dissociated tumor spheroids using anti-CD44 MicroBeads (Cat. No.130-095194, Miltenyi Biotec, Bergisch Gladbach, Germany) or anti-CD133 MicroBeads (Cat. No. 130-097-049, Miltenyi Biotec), respectively, according to the manufacturer's instructions. .. Briefly, tumor spheroids were enzymatically dissociated into single-cell suspensions using Accutase (Cat. No. A1110501; Life Technologies, Thermo Fisher Scientific) and incubated with anti-CD44 or anti-CD133 magnetic beads at 4 ◦C for 15 min.

    Article Title: Mfn1-mediated imbalanced mitochondrial dynamics promotes ovarian cancer stemness by inducing metabolic reprogramming.
    Article Snippet: properties contributing to cancer progression, disease recurrence, and drug resistance [1, 2].. Thus, understanding the precise molecular mechanism underpinning the stemness properties of ovarian cancer stem cells (OCSCs) will be relevant in developing effective strategies to combat ovarian cancer (OC).. CSCs have distinct metabolic properties compared to non-CSC tumor cells [3, 4].

    Article Title: CXCL8/SDC1 axis mediates tumor stem cell interactions to drive remote transfer in thyroid cancer
    Article Snippet: Tohoku Hospital Pediatrics-1 (THP-1) cells (CL-0233, Procell, Wuhan, China) were cultured in RPMI1640 medium containing 10% FBS and 1% penicillin/streptomycin. .. Once the FTC238 cells reached 90% confluency, CD133+cells were isolated using immunomagnetic beads (130-097-049, Miltenyi Biotec, Bergisch Gladbach, Germany), obtaining THCA stem cells (FTC238-S). ..

    Article Title: Glucose-induced histone lactylation confers metformin resistance in colorectal cancer stem cells.
    Article Snippet: .. Magnetic bead sorting of CD133+ cells CD133+ cells were isolated from SW620 and HCT15 cells using a CD133 MicroBead Kit (Miltenyi Biotec, 130-097-049). ..

    Article Title: hsa-miR-1246 is Consistently Overexpressed in Spheroid-Derived Cancer Stem Cells From Multiple Tumor Types
    Article Snippet: .. To confirm the presence of the CSC phenotype, we isolated the cells using the CD133 MicroBead Kit (human; Catalog #130-097-049 by Miltenyi Biotec), as CD133 is a marker of CSCs. .. Briefly, cells are labeled with CD133 MicroBeads, incubated, and then separated using a MACS column under a magnetic field.

    Marker:

    Article Title: hsa-miR-1246 is Consistently Overexpressed in Spheroid-Derived Cancer Stem Cells From Multiple Tumor Types
    Article Snippet: .. To confirm the presence of the CSC phenotype, we isolated the cells using the CD133 MicroBead Kit (human; Catalog #130-097-049 by Miltenyi Biotec), as CD133 is a marker of CSCs. .. Briefly, cells are labeled with CD133 MicroBeads, incubated, and then separated using a MACS column under a magnetic field.

    Inhibition:

    Article Title: An African ancestry-enriched H1 sub-haplotype challenges MAPT haplotype assignment and reveals distinct tau regulatory programs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER R v4.2.2 R Foundation https://www.r-project.org/ ImageJ Schneider et al.71 https://imagej.net/ij/ cutadapt Marcel et al.72 https://cutadapt.readthedocs.io/en/stable/ bowtie2 Langmead et al.73 https://github.com/benlangmead/bowtie2 samtools Li et al.70 http://www.htslib.org/ removeChrom.py Buenrostro et al.74 https://github.com/harvardinformatics/ATAC- seq/blob/master/atacseq/removeChrom.py WASP van de Geijn et al.75 https://www.encodeproject.org/software/ wasp/ MACS3 Zhang et al.76 https://macs3-project.github.io/MACS/ tssenrich PyPI Package https://gitlab.com/aaylward/tssenrich DiffBind Ross-Innes et al.77 https://bioconductor.posit.co/packages/3.20/ bioc/html/DiffBind.html edgeR Robinson et al.78 https://www.bioconductor.org/packages//2.7/ bioc/html/edgeR.html RUVSeq Risso et al.79 https://bioconductor.posit.co/packages/3.21/ bioc/html/RUVSeq.html Other Matrigel Corning Cat# 354230 BrainPhis StemCell technologies Cat# 05790 Neuronal Inducing Medium StemCell technologies Cat# 05839 CD133 MicroBead Kit, human Miltenyi Cat# 130-097-049 CD271 MicroBead Kit, human Miltenyi Cat# 130-099-023 Astrocyte media kit Sciencell Cat# 1801 Accutase Innovative Cell Technologies Cat# AT104 StemFlex ThermoFisher Scientific Cat# A3349401 N2 ThermoFisher Scientific Cat# 1750204 NEAA ThermoFisher Scientific Cat# 11140050 B27 without vitamin A ThermoFisher Scientific Cat# 12587010 glutamax ThermoFisher Scientific Cat# 35050061 Ascorbic acid Sigma Aldrich Cat# W210901 Releser StemCell Cat# 50-206-9324 cAMP Sigma Aldrich Cat# S-D0627-100MG BDNF R&D Cat# BT-248-BDB-010 GDNF R&D Cat# BT-212-GD-010 DMEM/F-12 with soduim pyruvate GlutaMAX ThermoFisher Scientific Cat# 10565018 penicillin/streptomycin ThermoFisher Scientific Cat# 15140122 FGF2 R&D Cat# BT-FGFB-020 bambanker FisherSci Cat# NC2960954 ll OPEN ACCESSArticle Neuron 115, 1–17.e1–e12, March 3, 2027 e4 Neuronal and astrocyte differentiation protocols iPSCs were differentiated into neural progenitor cells (NPCs) and then neuronal cultures as previously described,81 this method is also referred to as Dual SMAD inhibition differentiation. .. Briefly, iPSC were seeded in Neuronal Inducing Medium (05839 StemCell technologies) to form Rosettes.



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    COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 <t>CD133+</t> cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.
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    Miltenyi Biotec miltenyi biotec cd133 microbead
    COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 <t>CD133+</t> cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.
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    COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 <t>CD133+</t> cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.
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    Image Search Results


    COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 CD133+ cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.

    Journal: Genes to Cells

    Article Title: Functional Role of COP1 Gene in Hepatocellular Carcinoma Lipid Metabolism and Stemness

    doi: 10.1111/gtc.70108

    Figure Lengend Snippet: COP1 knockdown reduces motility, and stemness in HCC cells. (a‐b) Migration (a) or invasion (b) assay showing reduced motility in Huh7 and HepG2 cells treated with COP1‐siRNA, as quantified by the relative migration or invaded area. Statistical significance: *, p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. (c) Sphere formation assay images and quantitative data from Huh7 and HepG2 cells, demonstrating a decrease in both the number and size of spheres in COP1‐siRNA‐treated cells. (d) Sphere formation assay images and quantitative data from PLC/PRF/5 CD133+ cells. Compared to the control grpup, both the sphere number and size decreased upon COP1‐siRNA treatment. Scale bar, 100 μm Statistical significance: *, p < 0.05; *** p < 0.001 vs. control.

    Article Snippet: The cells were treated with FcR Blocking Reagent and CD133 MicroBeads (130‐100‐857, Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions and incubated for 15 min at 4°C in the dark.

    Techniques: Knockdown, Migration, Control, Tube Formation Assay