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nectin 2 pe  (Miltenyi Biotec)


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    Miltenyi Biotec nectin 2 pe
    Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and <t>Nectin-2,</t> respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).
    Nectin 2 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd112/CD112+Antibody%2C+anti-human%2C+REAfinity/pmc12901471-67-27-33
    Average 94 stars, based on 7 article reviews
    nectin 2 pe - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Osteoclasts affect the anti-cancer activity of NK cells"

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1730283

    Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).
    Figure Legend Snippet: Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Techniques Used: Expressing, Flow Cytometry

    Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).
    Figure Legend Snippet: Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Techniques Used: Co-Culture Assay, Expressing

    Related Articles

    Flow Cytometry:

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells
    Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% Penicillin-Streptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA). ..

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells
    Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% PenicillinStreptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA). ..

    Staining:

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells
    Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% Penicillin-Streptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA). ..

    Article Title: CD94-driven in vitro expansion of highly functional adaptive NKG2C + NKG2A - CD57 + NK cells from CMV + healthy donors
    Article Snippet: .. CD3 VioGreen REA613 Miltenyi Biotec CD3 PerCP-Cy5.5 UCHT1 BD Biosciences CD3z PE 6B10.2 eBioscience Inc. CD14 VioGreen/APC REA599 Miltenyi Biotec CD16 PerCP-Cy5.5 3G8 BD Biosciences CD19 VioGreen/VioBlue REA675 Miltenyi Biotec CD33 VioGreen REA775 Miltenyi Biotec CD34 APC QBEnd10 R&D Systems CD34 PE QBEnd10 Beckman Coulter CD48 - CO202 Produced in our laboratory CD56 PC7 N901 Beckman Coulter CD56 - C218 Produced in our laboratory CD57 VioBlue TB03 Miltenyi Biotec CD107a (LAMP1) PE/APC-H7 H4A3 BD Biosciences FcεRI-γ FITC Polyclonal Merck Millipore Fixable Viability Stain BV510 - BD Biosciences Granzyme B PE GB12 Invitrogen Goat anti-mouse IgG1 PE Polyclonal SouthernBiotech Goat anti-mouse IgG2b PE/APC-Cy7 Polyclonal SouthernBiotech HLA-E - 3D12 Abnova HLA-DR - D1.12 Produced in our laboratory HLA-G - MEM-G/9 Abnova HLA-I - W6/32 Produced in our laboratory IFN-γ APC/PE B27 BD Biosciences KIR2DL1/S1-2DL3*005 FITC/APC 11PB6 Miltenyi Biotec KIR2DL1/S5 FITC/APC/PE 143211 R&D Systems KIR2DL2/L3/S2 APC DX27 Miltenyi Biotec KIR2DL2/L3/S2 FITC CH-L BD Biosciences KIR2DL2/L3/S2 PE GL183 Beckman Coulter KIR3DL1 FITC DX9 Miltenyi Biotec LAG-3 APC REA351 Miltenyi Biotec LFA-3 - TS2/9 Produced in our laboratory MIC-A - BAM195 Kindly provided by Prof. Pende Nectin2 - L14 Produced in our laboratory NKG2A APC Z199 Beckman Coulter NKG2C - 134522 R&D Systems NKG2C APC 134591 R&D Systems PD-1 PE PD1.3.1.3 Miltenyi Biotec Perforin PerCP-Cy5.5 DG9 BD Biosciences PVR - 5A.10 Produced in our laboratory Syk FITC 4D10 BD Biosciences TIGIT - 741182 R&D Systems TIM-3 APC REA635 Miltenyi Biotec TNF-a PE REA656 Miltenyi Biotec ULBP1 - 170818 R&D Systems ULBP2 - 165903 R&D Systems ULBP3 - 166510 R&D Systems ULBP4 - 709116 R&D Systems ..

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells
    Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% PenicillinStreptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA). ..

    Bioprocessing:

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells
    Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% Penicillin-Streptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA). ..

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells
    Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% PenicillinStreptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA). ..

    Produced:

    Article Title: CD94-driven in vitro expansion of highly functional adaptive NKG2C + NKG2A - CD57 + NK cells from CMV + healthy donors
    Article Snippet: .. CD3 VioGreen REA613 Miltenyi Biotec CD3 PerCP-Cy5.5 UCHT1 BD Biosciences CD3z PE 6B10.2 eBioscience Inc. CD14 VioGreen/APC REA599 Miltenyi Biotec CD16 PerCP-Cy5.5 3G8 BD Biosciences CD19 VioGreen/VioBlue REA675 Miltenyi Biotec CD33 VioGreen REA775 Miltenyi Biotec CD34 APC QBEnd10 R&D Systems CD34 PE QBEnd10 Beckman Coulter CD48 - CO202 Produced in our laboratory CD56 PC7 N901 Beckman Coulter CD56 - C218 Produced in our laboratory CD57 VioBlue TB03 Miltenyi Biotec CD107a (LAMP1) PE/APC-H7 H4A3 BD Biosciences FcεRI-γ FITC Polyclonal Merck Millipore Fixable Viability Stain BV510 - BD Biosciences Granzyme B PE GB12 Invitrogen Goat anti-mouse IgG1 PE Polyclonal SouthernBiotech Goat anti-mouse IgG2b PE/APC-Cy7 Polyclonal SouthernBiotech HLA-E - 3D12 Abnova HLA-DR - D1.12 Produced in our laboratory HLA-G - MEM-G/9 Abnova HLA-I - W6/32 Produced in our laboratory IFN-γ APC/PE B27 BD Biosciences KIR2DL1/S1-2DL3*005 FITC/APC 11PB6 Miltenyi Biotec KIR2DL1/S5 FITC/APC/PE 143211 R&D Systems KIR2DL2/L3/S2 APC DX27 Miltenyi Biotec KIR2DL2/L3/S2 FITC CH-L BD Biosciences KIR2DL2/L3/S2 PE GL183 Beckman Coulter KIR3DL1 FITC DX9 Miltenyi Biotec LAG-3 APC REA351 Miltenyi Biotec LFA-3 - TS2/9 Produced in our laboratory MIC-A - BAM195 Kindly provided by Prof. Pende Nectin2 - L14 Produced in our laboratory NKG2A APC Z199 Beckman Coulter NKG2C - 134522 R&D Systems NKG2C APC 134591 R&D Systems PD-1 PE PD1.3.1.3 Miltenyi Biotec Perforin PerCP-Cy5.5 DG9 BD Biosciences PVR - 5A.10 Produced in our laboratory Syk FITC 4D10 BD Biosciences TIGIT - 741182 R&D Systems TIM-3 APC REA635 Miltenyi Biotec TNF-a PE REA656 Miltenyi Biotec ULBP1 - 170818 R&D Systems ULBP2 - 165903 R&D Systems ULBP3 - 166510 R&D Systems ULBP4 - 709116 R&D Systems ..

    CRISPR:

    Article Title: Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function
    Article Snippet: The cells were cultured in IMDM (Gibco) supplemented with FBS (20%), 2-mercaptoethanol (0.08%), and penicillin-streptomycin (1%). .. For CD155 and CD112 KO variants, deletion of PVR and/or NECTIN2 was performed using CRISPR/Cas9 followed by magnetic-based negative selection using LS and LD columns (Miltenyi). ..

    Selection:

    Article Title: Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function
    Article Snippet: The cells were cultured in IMDM (Gibco) supplemented with FBS (20%), 2-mercaptoethanol (0.08%), and penicillin-streptomycin (1%). .. For CD155 and CD112 KO variants, deletion of PVR and/or NECTIN2 was performed using CRISPR/Cas9 followed by magnetic-based negative selection using LS and LD columns (Miltenyi). ..

    other:


    Diagnostic Assay:

    Article Title: Colorectal cancer-associated fibroblasts inhibit effector T cells via NECTIN2 signaling.
    Article Snippet: .. Original Articles Colorectal cancer-associated fibroblasts inhibit effector T cells via NECTIN2 signaling David J. Agorku a,b, Andreas Bosio a, Frauke Alves c,d,e, Philipp Ströbel b, Olaf Hardt a,* a Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany b University Medical Center Göttingen (UMG), Institute of Pathology, Göttingen, Lower Saxony, Germany c University Medical Center Göttingen, Department of Hematology and Medical Oncology, Göttingen, Lower Saxony, Germany d University Medical Center Göttingen, Institute for Diagnostic and Interventional Radiology, Göttingen, Lower Saxony, Germany e Max Planck Institute for Multidisciplinary Sciences, Translational Molecular Imaging, Göttingen, Lower Saxony, Germany A R T I C L E I N F O Keywords: Colorectal cancer Cancer-associated fibroblasts Fibroblast heterogeneity NECTIN2 TinCAFs A B S T R A C T Cancer-associated fibroblasts play a crucial role within the tumor microenvironment. ..

    Imaging:

    Article Title: Colorectal cancer-associated fibroblasts inhibit effector T cells via NECTIN2 signaling.
    Article Snippet: .. Original Articles Colorectal cancer-associated fibroblasts inhibit effector T cells via NECTIN2 signaling David J. Agorku a,b, Andreas Bosio a, Frauke Alves c,d,e, Philipp Ströbel b, Olaf Hardt a,* a Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany b University Medical Center Göttingen (UMG), Institute of Pathology, Göttingen, Lower Saxony, Germany c University Medical Center Göttingen, Department of Hematology and Medical Oncology, Göttingen, Lower Saxony, Germany d University Medical Center Göttingen, Institute for Diagnostic and Interventional Radiology, Göttingen, Lower Saxony, Germany e Max Planck Institute for Multidisciplinary Sciences, Translational Molecular Imaging, Göttingen, Lower Saxony, Germany A R T I C L E I N F O Keywords: Colorectal cancer Cancer-associated fibroblasts Fibroblast heterogeneity NECTIN2 TinCAFs A B S T R A C T Cancer-associated fibroblasts play a crucial role within the tumor microenvironment. ..



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    Image Search Results


    Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Journal: Frontiers in Immunology

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells

    doi: 10.3389/fimmu.2026.1730283

    Figure Lengend Snippet: Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Article Snippet: After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA).

    Techniques: Expressing, Flow Cytometry

    Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Journal: Frontiers in Immunology

    Article Title: Osteoclasts affect the anti-cancer activity of NK cells

    doi: 10.3389/fimmu.2026.1730283

    Figure Lengend Snippet: Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).

    Article Snippet: After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Nectin-2 PE, Nectin-4 Vio Bright V423 (Miltenyi Biotec, Bergisch Gladbach, Germany), CXCR4 APC (BD Bioscence, Franklin Lakes, New Jersey, USA), PVR BV510 (Biolegend, San Diego, USA).

    Techniques: Co-Culture Assay, Expressing