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itgb4  (R&D Systems)


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    Structured Review

    R&D Systems itgb4
    Itgb4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd104/Human+Integrin+beta+4%2FCD104+Antibody/pmc11080809-83-49-65
    Average 93 stars, based on 10 article reviews
    itgb4 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Extracellular Vesicle Antibody Microarray for Multiplexed Inner and Outer Protein Analysis
    Article Snippet: The following trios of barcoded antibodies were used for triplexed detection: CD63-BC38, ITG α2 (MAB1233, R&D Systems)-BC64 and HSP70-BC23; CD9-BC51, ITG α6 (MAB1350, R&D Systems)-BC09 and Alix (MA1-83977, Invitrogen)-BC60; CD81-BC50, ITG β1 (MAB17781, R&D Systems)-BC27, HSP90-BC60; CD82 (342102, Biolegend)-BC43, ITG β4 (MAB4060, R&D Systems)-BC79, claudin-2 (32-5600, ThermoFisher)-BC61.

    Article Title: Extracellular Vesicle Antibody Microarray for Multiplexed Inner and Outer Protein Analysis.
    Article Snippet: The following trios of barcoded antibodies were used for triplexed detection: CD63-BC38, ITG α2 (MAB1233, R&D Systems)-BC64, and HSP70-BC23; CD9-BC51, ITG α6 (MAB1350, R&D Systems)-BC09, and Alix (MA1-83977, Invitrogen)-BC60; CD81-BC50, ITG β1 (MAB17781, R&D Systems)-BC27, and HSP90-BC60; CD82 (342102, Biolegend)-BC43, ITG β4 (MAB4060, R&D Systems)-BC79, and claudin-2 (32−5600, Thermo Fisher)-BC61.

    Blocking Assay:

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis
    Article Snippet: Fluorescent PLA on cells was performed using Duolink in situ detection reagent (far red, Sigma-Aldrich, #DUO92008) according to manufacturer’s protocol. .. Briefly, slides were blocked with blocking solution for 30 minutes at RT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) and mouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500 μg ml -1 stock) at 4 °C overnight. .. Duolink in situ anti-rabbit minus and anti-mouse plus (Sigma-Aldrich, #DUO92008, DUO92001) were used as secondary antibodies.

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis.
    Article Snippet: Fluorescent PLA on cells was performed using Duolink in situ detection reagent (far red, Sigma-Aldrich, #DUO92008) according to manufacturer’s protocol. .. Briefly, slideswereblockedwith blocking solution for 30minutes atRT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) andmouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500μgml-1 stock) at 4 °C overnight. .. Duolink in situ anti-rabbit minus and antimouse plus (Sigma-Aldrich, #DUO92008, DUO92001) were used as secondary antibodies.

    Incubation:

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis
    Article Snippet: Fluorescent PLA on cells was performed using Duolink in situ detection reagent (far red, Sigma-Aldrich, #DUO92008) according to manufacturer’s protocol. .. Briefly, slides were blocked with blocking solution for 30 minutes at RT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) and mouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500 μg ml -1 stock) at 4 °C overnight. .. Duolink in situ anti-rabbit minus and anti-mouse plus (Sigma-Aldrich, #DUO92008, DUO92001) were used as secondary antibodies.

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis.
    Article Snippet: Fluorescent PLA on cells was performed using Duolink in situ detection reagent (far red, Sigma-Aldrich, #DUO92008) according to manufacturer’s protocol. .. Briefly, slideswereblockedwith blocking solution for 30minutes atRT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) andmouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500μgml-1 stock) at 4 °C overnight. .. Duolink in situ anti-rabbit minus and antimouse plus (Sigma-Aldrich, #DUO92008, DUO92001) were used as secondary antibodies.

    Bioprocessing:

    Article Title: Integrins are enriched on aberrantly fucosylated tumour‐derived urinary extracellular vesicles
    Article Snippet: RPMI‐1640 were purchased from Lonza (Belgium) and 10% fetal bovine serum (FBS) was acquired from GIBCO (USA). .. Monoclonal antibodies anti‐ITGA1 (clone 639508) (catalog # MAB5676), ‐ITGA2 (clone HAS3) (catalog # MAB1233), ‐ITGA3 (clone IA3) (catalog # MAB1345), ‐ITGA5 (clone 612557) (catalog # MAB18642), ‐ITGA6 (clone MP4F10) (catalog # MAB1350), ‐ITGAV (clone 273210) (catalog # MAB1219), ‐ITGAVB3 (clone 23C6) (catalog # MAB3050), ‐ITGB1 (4B7R) (catalog # MAB1778), ‐ITGB4 (clone 422325) (catalog # MAB4060) and ‐CD9 (clone 209306) (catalog # MAB1880) were purchased from R&D systems (Abingdon, UK). .. Other monoclonal antibodies anti‐ITGAM (clone ICRF44) (catalog # 555386), ‐ITGAX (clone 3.9) (catalog # 565805), ‐ITGB2 (clone 6.7) (catalog # 555922), ‐ITGB3 (clone VI‐PL2) (catalog # 555752), and ‐CD63 (clone 556019) (catalog # 556019) were purchased from BD Bioscience (Vantaa, Finland).



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    Image Search Results


    Journal: eLife

    Article Title: The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis

    doi: 10.7554/eLife.86931

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-integrin β4/CD104 (rat monoclonal 439-9B) , eBioscience , 14-1049-82 RRID: AB_1210460 , FACS (1:100).

    Techniques: Plasmid Preparation, Control, Sequencing, Negative Control, Recombinant, Software, Transfection, Histopathology, Western Blot, Staining, Permeability

    Journal: eLife

    Article Title: The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis

    doi: 10.7554/eLife.86931

    Figure Lengend Snippet:

    Article Snippet: Antibodies were Δ-HS (F69-3G10, AMS Biotechnology), CS (CS56, Merck), HSPG2/perlecan (7B5, ThermoFisher Scientific), glypican-1 (AF4519), integrin β4/CD104 (clone 439-9B, eBioscience), integrin β1/CD29 (P4C10, NBP2-36561), syndecan-2 (MAB2965), biglycan (AF2667), laminin α5 (NBP2-42391) from Biotechne.

    Techniques: Plasmid Preparation, Control, Sequencing, Negative Control, Recombinant, Software, Transfection, Histopathology, Western Blot, Staining, Permeability