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anti mcd103 m290  (Bio X Cell)


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    Structured Review

    Bio X Cell anti mcd103 m290
    In vivo <t>anti-mCD103</t> mAb microPET imaging. (A) Representative coronal <t>89Zr-anti-M290</t> and 89Zr-isotype control PET scans, 1, 3 and 6 days post tracer injection in non-tumor bearing C57BL/6 mice. (B) Ex vivo tissue biodistribution of 89Zr-anti-M290 and 89Zr-isotype control in non-tumor bearing C57BL/6 mice, 6 days post-tracer injection (median, n=3). (C) Analysis of ex vivo macroscopic tracer distribution in FFPE tumor tissue slices using autoradiography. (D) immunohistochemical CD103 staining of the small intestine, mesenteric lymph node (left) and colon (right). ALN, axillary lymph node; BLN, brachial lymph node; MLN, mesenteric lymph node.
    Anti Mcd103 M290, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd103/InVivoMAb+anti-mouse+CD103/pmc09723959-150-0-2
    Average 93 stars, based on 24 article reviews
    anti mcd103 m290 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Development of 89 Zr-anti-CD103 PET imaging for non-invasive assessment of cancer reactive T cell infiltration"

    Article Title: Development of 89 Zr-anti-CD103 PET imaging for non-invasive assessment of cancer reactive T cell infiltration

    Journal: Journal for Immunotherapy of Cancer

    doi: 10.1136/jitc-2022-004877

    In vivo anti-mCD103 mAb microPET imaging. (A) Representative coronal 89Zr-anti-M290 and 89Zr-isotype control PET scans, 1, 3 and 6 days post tracer injection in non-tumor bearing C57BL/6 mice. (B) Ex vivo tissue biodistribution of 89Zr-anti-M290 and 89Zr-isotype control in non-tumor bearing C57BL/6 mice, 6 days post-tracer injection (median, n=3). (C) Analysis of ex vivo macroscopic tracer distribution in FFPE tumor tissue slices using autoradiography. (D) immunohistochemical CD103 staining of the small intestine, mesenteric lymph node (left) and colon (right). ALN, axillary lymph node; BLN, brachial lymph node; MLN, mesenteric lymph node.
    Figure Legend Snippet: In vivo anti-mCD103 mAb microPET imaging. (A) Representative coronal 89Zr-anti-M290 and 89Zr-isotype control PET scans, 1, 3 and 6 days post tracer injection in non-tumor bearing C57BL/6 mice. (B) Ex vivo tissue biodistribution of 89Zr-anti-M290 and 89Zr-isotype control in non-tumor bearing C57BL/6 mice, 6 days post-tracer injection (median, n=3). (C) Analysis of ex vivo macroscopic tracer distribution in FFPE tumor tissue slices using autoradiography. (D) immunohistochemical CD103 staining of the small intestine, mesenteric lymph node (left) and colon (right). ALN, axillary lymph node; BLN, brachial lymph node; MLN, mesenteric lymph node.

    Techniques Used: In Vivo, Imaging, Control, Injection, Ex Vivo, Autoradiography, Immunohistochemical staining, Staining

    Related Articles

    other:

    Article Title: Crystalline silica-induced recruitment and immuno-imbalance of CD4 + tissue resident memory T cells promote silicosis progression
    Article Snippet: Anti-CD103 treatment: Anti-mouse CD103 (M290, BE0026) and isotype-matched antibodies were purchased from Bio X cell.

    Article Title: Crystalline silica-induced recruitment and immuno-imbalance of CD4 + tissue resident memory T cells promote silicosis progression.
    Article Snippet: Anti-CD103 treatment: Anti-mouse CD103 (M290, BE0026) and isotypematched antibodies were purchased from Bio X cell.

    Article Title: Antibody-ALK5 inhibitor conjugates and their uses
    Article Snippet: Examples of antibodies that bind to T cell surface molecules and believed to be internalizing include OKT6 (anti-CD1; ATCC deposit no. CRL8020), OKT11 (anti-CD2; ATCC deposit no. CRL8027); OKT3 (anti-CD3; ATCC deposit no. CRL8001); OKT4 (anti-CD4; ATCC deposit no. CRL8002); OKT8 (anti-CD8; ATCC deposit no. CRL8014); 7D4 (anti-CD25; ATCC deposit no. CRL1698); OKT9 (anti-CD71; ATCC deposit no. CRL8021); CD28.2 (anti-CD28, BD Biosciences Cat. No. 556620); UCHT1 (anti-CD3, BioXCell Cat. No. BE0231); M290 (anti-CD103, BioXCell Cat. No. BE0026); and FR70 (anti-CD70, BioXCell Cat. No. BE0022).

    Control:

    Article Title: Different tumour-resident memory T-cell subsets regulate responses to anti-PD-1 and anti-CTLA-4 cancer immunotherapies.
    Article Snippet: A negative isotype control (IgG2a, clone 2A3, Bio-X-Cell BE0089; polyclonal Syrian hamster IgG, Bio-X-Cell BE0087) was included. .. Animals were challenged i.p. with 100μg/mouse anti-CD8 (clone 2.43, Bio-X-Cell BE0061) or anti-CD4 (clone GK1.5, Biolegend 900004676) neutralizing mAb, or received isotype control (IgG2b, clone LTF-2, Bio-X-Cell BE0090; IgG2b, clone RTK4530, Biolegend 400602).We injected 50μg/mouseof anti-CD103 (clone M290, Bio-X-Cell BE0026) or anti-CD49a (clone Ha31/8, BD Pharmingen, 555000) neutralizing mAb, or isotype control (IgG2a, clone 2A3, Bio-X-Cell BE0089; Armenian Hamster IgG2, clone Ha4/8, BD Parmingen, 553961) into the tumour. ..

    Article Title: Visualizing the spatial organization of monocytes, interstitial macrophages, and tissue-specific macrophages in situ.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies InVivoMab anti-mouse CSF1R BioXcell Cat#BE0213; RRID:AB_2687699 InVivoMab rat IgG2a isotype control, anti-trinitrophenol Bioxcell Cat#BE0089; RRID:AB_1107769 InVivoMab anti-mouse CSF1 BioXcell Cat#BE0204; RRID:AB_10950309 InVivoMab anti-mouse GM-CSF BioXcell Cat#BE0259; RRID: AB_2687738 InVivoMab rat IgG1 isotype control, anti-horseradish peroxidase BioXcell Cat#BE088; RRID:AB_1107775 PerCP-Cy5.5 Anti-mouse CD45 (Clone: 30-F11) BD Biosciences Cat# 550995; RRID:AB_394003 PE Anti-mouse MHCII (Clone: AF6-120.1) BD Biosciences Cat# 553552; RRID:AB_394919 BUV395 Anti-mouse CD11b (Clone: M1/70) BD Biosciences Cat#563553; RRID: AB_2738276 Brillant Violet 750 Anti-mouse CCR2 (Clone: 475301) BD Biosciences Cat#747967; RRID: AB_2872428 APC Anti-mouse FOLR2 (Clone: 10/FR2) Biolegend Cat#153306; RRID:AB_2721313 BUV563 Anti-mouse Ly-6G (Clone: 1A8) BD Biosciences Cat#612921; RRID:AB_2870206 PE Anti-mouse CD88 (Clone: 20/70) Biolegend Cat#135806; RRID:AB_2243735 Alexa Fluor 647 Anti-mouse CD31 (Clone: 390) Biolegend Cat#102416; RRID:AB_493410 APC/Fire 810 Anti-mouse CD45 (Clone: 30-F11) Biolegend Cat#103174; RRID:AB_2860600 Alexa Fluor 647 Anti-mouse CLEC4F (Clone: 3E3F9) Biolegend Cat#156804; RRID:AB_2814082 eFluor 450 Anti-mouse LYVE1 (Clone: ALY7) eBioscience Cat#48-0443-82; RRID: AB_2784723 Brillant Violet 650 Anti-mouse CD206 (Clone: C068C2) Biolegend Cat#141723; RRID:AB_2562445 Alexa Fluor 647 Anti-mouse CD39 (Clone: Duha59) Biolegend Cat#143808; RRID:AB_2563978 PerCP Anti-mouse Ly-6C (Clone: HK1.4) Biolegend Cat#128028; RRID:AB_10897805 Pe/Cyanine7 Anti-mouse MGL1 (Clone: LOM-8.7) Biolegend Cat#145609; RRID:AB_2566028 BUV661 Anti-mouse CD103 (Clone: M290) BD Biosciences Cat#741504; RRID:AB_2870960 Brillant Violet 510 Anti-mouse I-A/I-E (Clone: M5/114.15.2) Biolegend Cat#107635; RRID:AB_2561397 Brillant Violet 570 Anti-mouse CD11c (Clone: N418) Biolegend Cat#117331; RRID:AB_10900261 BB700 Anti-mouse Sirpa (Clone: P84) BD Biosciences Cat#742205; RRID:AB_2871427 (Continued on next page) Cell Reports 43, 114847, October 22, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Brillant Violet 605 Anti-mouse TIM4 (Clone: RMT4-54) BD Biosciences Cat#745206; RRID:AB_2742799 APC Anti-mouse CD163 (Clone: S15049I) Biolegend Cat#155305; RRID:AB_2814059 Brillant Violet 421 Anti-mouse CD163 (Clone: S15049I) Biolegend Cat#155309; RRID:AB_2814063 PE-CF594 Anti-mouse Siglec-F (Clone: E50-2440) BD Biosciences Cat#562757; RRID:AB_2687994 BUV737 Anti-mouse CD43 (Clone: S7) BD Biosciences Cat#612840; RRID:AB_2870162 Brillant Violet 711 Anti-mouse CX3CR1 (Clone: SA011F11) Biolegend Cat#149031; RRID:AB_2565939 APC-R700 Anti-mouse F4/80 (Clone: T45-2342) BD Biosciences Cat#565787; RRID:AB_2869711 Brillant Violet 786 Anti-mouse CD64 (Clone: X54-5/7.1) BD Biosciences Cat#741024; RRID:AB_2740644 ViaDye Red Fixable Viability Dye Kit Cytek Cat#SKU R7-60008 Biotin Anti-mouse NK-1.1 (Clone: PK136) BD Biosciences Cat#553163; RRID:AB_394675 Biotin Anti-mouse CD3e (Clone: 500A2) BD Biosciences Cat#553239; RRID:AB_394728 Biotin Anti-mouse CD19 (Clone: 1D3) BD Biosciences Cat#553784; RRID:AB_395048 Biotin Anti-mouse CD5 (Clone: 53–7.3) BD Biosciences Cat#553019; RRID:AB_394557 Biotin Anti-mouse CD4 (Clone: GK1.5) BD Biosciences Cat#553728; RRID:AB_395012 Biotin Anti-mouse CD45R/B220 (Clone: RA3-6B2) BD Biosciences Cat#553086; RRID:AB_394615 Biotin Anti-mouse CD8a (Clone: 53–6.7) BD Biosciences Cat#553029; RRID:AB_394566 PE-Cy5 Streptavidin BD Biosciences Cat#554062; RRID:AB_10053563 Chemicals, peptides, and recombinant proteins Dnase I Roche Cat#10104159001 Dispase II Gibco Cat#17105041 Collegenase IV Gibco Cat#17104019 Tissue freezing medium (OCT) Microm-Microtech Cat#F/TFM-C Polybeads carboxylate microsphere Polysciences Cat#07759-15 Sucrose Biosolve Cat#192223 Vectashield with DAPI VectorLabs Cat#H-1200-10 Cyclophosphamide monohydrate Sigma-Aldrich Cat#C0768 True-Stain Monocyte Blocker Biolegend Cat#426103 Evans Blue Sigma Aldrich Cat#314-13-6 Mouse BD Fc Block BD Biosciences Cat#553141; RRID:AB_394656 Formaldehyde Sigma Cat#47608 Experimental models: Organisms/strains Mouse: Cx3cr1EGFP/Kin The Jackson laboratory RRID:IMSR_JAX:005582 Mouse: Csf1rmApple https://doi.org/10.4049/jimmunol.1701488 Developed by D. Hume Mouse: DCsf1rECFP https://doi.org/10.1189/jlb.0807585 Developed by D. Hume Mouse: Ccr2 / The Jackson laboratory RRID:IMSR_JAX:004999 (Continued on next page) 18 Cell Reports 43, 114847, October 22, 2024

    Article Title: Different tumour-resident memory T-cell subsets regulate responses to anti-PD-1 and anti-CTLA-4 cancer immunotherapies
    Article Snippet: Animals were challenged i.p. with 100 μg/mouse anti-CD8 (clone 2.43, Bio-X-Cell BE0061) or anti-CD4 (clone GK1.5, Biolegend 900004676) neutralizing mAb, or received isotype control (IgG2b, clone LTF-2, Bio-X-Cell BE0090; IgG2b, clone RTK4530, Biolegend 400602). .. We injected 50 μg/mouse of anti-CD103 (clone M290, Bio-X-Cell BE0026) or anti-CD49a (clone Ha31/8, BD Pharmingen, 555000) neutralizing mAb, or isotype control (IgG2a, clone 2A3, Bio-X-Cell BE0089; Armenian Hamster IgG2, clone Ha4/8, BD Parmingen, 553961) into the tumour. ..

    Injection:

    Article Title: Different tumour-resident memory T-cell subsets regulate responses to anti-PD-1 and anti-CTLA-4 cancer immunotherapies.
    Article Snippet: A negative isotype control (IgG2a, clone 2A3, Bio-X-Cell BE0089; polyclonal Syrian hamster IgG, Bio-X-Cell BE0087) was included. .. Animals were challenged i.p. with 100μg/mouse anti-CD8 (clone 2.43, Bio-X-Cell BE0061) or anti-CD4 (clone GK1.5, Biolegend 900004676) neutralizing mAb, or received isotype control (IgG2b, clone LTF-2, Bio-X-Cell BE0090; IgG2b, clone RTK4530, Biolegend 400602).We injected 50μg/mouseof anti-CD103 (clone M290, Bio-X-Cell BE0026) or anti-CD49a (clone Ha31/8, BD Pharmingen, 555000) neutralizing mAb, or isotype control (IgG2a, clone 2A3, Bio-X-Cell BE0089; Armenian Hamster IgG2, clone Ha4/8, BD Parmingen, 553961) into the tumour. ..

    Article Title: Fgl2-knockout tumor cells serve as a vaccine inducing long-duration brain-resident memory T cells that reject subsequent intracranial tumor cell challenges.
    Article Snippet: .. Anti-CD8α (clone 2.43), anti-CD69 (clone CD69.2.2), anti-CD103 (clone M290) (BioXCell West Lebanon, NH), or their matched IgG isotype was injected at a dose of 150 μg per mouse, 2 days before tumor inoculation and every 4 days thereafter. ..

    Article Title: Different tumour-resident memory T-cell subsets regulate responses to anti-PD-1 and anti-CTLA-4 cancer immunotherapies
    Article Snippet: Animals were challenged i.p. with 100 μg/mouse anti-CD8 (clone 2.43, Bio-X-Cell BE0061) or anti-CD4 (clone GK1.5, Biolegend 900004676) neutralizing mAb, or received isotype control (IgG2b, clone LTF-2, Bio-X-Cell BE0090; IgG2b, clone RTK4530, Biolegend 400602). .. We injected 50 μg/mouse of anti-CD103 (clone M290, Bio-X-Cell BE0026) or anti-CD49a (clone Ha31/8, BD Pharmingen, 555000) neutralizing mAb, or isotype control (IgG2a, clone 2A3, Bio-X-Cell BE0089; Armenian Hamster IgG2, clone Ha4/8, BD Parmingen, 553961) into the tumour. ..



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