casein blocking reagent (Bio-Rad)

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Bio-Rad casein blocking reagent
Casein Blocking Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casein+tbs+blocking+buffer/1x+Tris+Buffered+Saline+(TBS)+with+1%25+Casein/pm40136658-50-5-9
Average 95 stars, based on 122 article reviews

casein blocking reagent - by Bioz Stars, 2026-09

95/100 stars

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Saline:

Article Title: Analyses of astrocyte-neuron lactate shuttle transporter levels in brain tissues from people with HIV-associated neurocognitive impairment and Alzheimer’s disease
Article Snippet: After the transfer, total protein was imaged using a Bio-Rad ChemiDoc imager under the stain-free blot setting to control the transfer quality. .. The membranes were then blocked with 1 × TrisBuffered Saline with 1 % Casein (Bio-Rad, Cat. no: 1610782) for 1 h before incubation overnight at 4 °C with the primary antibody ( ). .. Anti-rabbit IgG, HRP-linked antibody was used as the secondary antibody for 1 h at room temperature (Bio-Rad, cat. no. 170–6516; 1:5000 in Phosphate-buffered saline (PBS)-Twin), visualized with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, Cat. no. 34095).

Article Title: Analyses of astrocyte-neuron lactate shuttle transporter levels in brain tissues from people with HIV-associated neurocognitive impairment and Alzheimer’s disease
Article Snippet: After the transfer, total protein was imaged using a Bio-Rad ChemiDoc imager under the stain-free blot setting to control the transfer quality. .. The membranes were then blocked with 1 × Tris Buffered Saline with 1 % Casein (Bio-Rad, Cat. no: 1610782) for 1 h before incubation overnight at 4 ◦C with the primary antibody (Table 3). .. Anti-rabbit IgG, HRP-linked antibody was used as the secondary antibody for 1 h at room temperature (Bio-Rad, cat. no. 170–6516; 1:5000 in Phosphatebuffered saline (PBS)-Twin), visualized with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, Cat. no. 34095).

Article Title: Pro-inflammatory LPS drives production and release of the chemokine MCP-1 in human coronary artery smooth muscle cells.
Article Snippet: Media (1 μl) were spotted on nitrocellulose membranes (Bio-Rad, cat. no. 1620168) using PBS as blank. .. Membranes were blocked in tris-buffered saline with 1% casein (Bio-Rad, cat. no. 1610782) for 1 h and incubated with a rabbit polyclonal MCP-1 antibody from Abcam (cat. no. Ab9669, 1:2000) for 24–48 h at 4 °C. .. Membranes were carefully washed in tris-buffered saline with 0.1% tween 20 and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling, cat. no. 7074) at a dilution 1:5000 for 2 h. After washing, membranes were incubated with West Femto chemiluminescence reagent from Thermo Fisher Scientific (cat. no. 34096), and MCP-1 immunoreactivity visualized and analyzed with a LI-COR Odyssey instrument (LI-COR Biosciences).

Article Title: TiO 2 -based adsorption platform for non-invasive capture and quantification of cell-derived secretome biomarkers.
Article Snippet: Extended author information available on the last page of the article Abstract Robust cell manufacturing demands minimally invasive methods to enrich and quantify representative secretome biomarkers, thereby providing critical insights into cellular phenotype and therapeutic potency.. Here, we evaluated a titanium dioxide (TiO2)-based batch adsorption platform for the non-invasive capture and quantification of secretome-associated biomolecules from human embryonic kidney 293T (HEK293T) and bone marrow-derived mesenchymal stem/stromal cell (MSC) cultures.. Leveraging TiO2’s affinity for phosphate-containing biomolecules, we first evaluated TiO2 powders and microspheres using standardized green fluorescent protein (GFP)-labeled extracellular vesicles (EVs) to benchmark adsorption behavior, and subsequently applied the workflow to capture phosphatidylcholine (PC) and extracellular ribonucleic acid (exRNA) directly from conditioned media.

Article Title: PCSK9 inhibition ameliorates microplastic-induced endothelial redox imbalance via SIRT6 modulation.
Article Snippet: .. Then, the membranes were blocked in 1× Tris-buffered saline (TBS) 1% casein blocker (1610782, Bio-Rad, Hercules, CA, USA) for 1 h at room temperature and incubated with primary antibodies against PCSK9 (1:1000, ab28770, Abcam, Cambridge, UK), sirtuin 6 (SIRT6, 1:1000, ab62739, Abcam, Cambridge, UK), p62 (1:500, sc-28359, Cell Signaling Technology, Danvers, MA, USA), ATG5 (1:500, E-AB-10814, Elabscience Biotechnology Inc., Houston, TX, USA), and Forkhead Box O3 (FOXO3A, 1:1000, Orb161062) overnight at 4 °C. ..

Incubation:

Article Title: Progesterone-driven stabilization of hybrid E/M states in amniotic epithelial cells enhances regeneration and immune modulatory capacities
Article Snippet: Membranes were blocked with EveryBlotTM Blocking Buffer (#12010020; Bio-Rad Laboratories, Milan, Italy) for 5 minutes. .. Primary antibodies against Polyclonal Goat anti-Human CDH1/E Cadherin Antibody (aa750-850, WB) (1:300; LS-C204222; LSBio, Newark, CA 94560 USA), Jagged1 (D4Y1R) XP® Rabbit mAb (1:300; #70109T; Cell Signaling technology, Danvers, MA 01923, USA), Nrf2 (1:300; NBP1-32822; Novus Biologicals, Centennial, CO 80112 USA), Anti-alpha smooth muscle Actin, clone 1A4 (1:300; ab7817, Abcam, Cambridge, UK), Vimentin, clone V9 (1:300; M0725; Dako, Santa Clara, CA 95051, USA), α-Tubulin, clone DM1A (1:300; #3873; Cell Signaling technology, Danvers, MA 01923, USA), Oct4 (1:300; ab18976; Abcam, Cambridge, UK), Sox2 (1:300; ab59776; Abcam, Cambridge, UK), Nanog (1:300; AB9220; Sigma-Aldrich, St. Louis, MO, USA), Tenomodulin (1:300; ab203676; Abcam, Cambridge, UK), Collagen I (1:300; ab292; Abcam, Cambridge, UK), CD86, BU63 (1:300; MCA1118SBV610; Bio-Rad Laboratories, Milan, Italy), MMR/CD206 (1:300; AF2535; R&D Systems, Minneapolis, MN 55413, USA) were diluted in 1× TBS containing 1% Casein Blocker (#1610782; Bio-Rad Laboratories, Milan, Italy) and incubated overnight at 4 °C. .. The HRP-conjugated anti-goat (1:5000; #SC-2354; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), HRP-conjugated anti-mouse (1:10000; #NA931; Cytiva, Marlborough, MA, USA NA931), and HRP-conjugated anti-rabbit (1:10000; #NA934; Cytiva, Marlborough, MA, USA NA931) secondary antibodies were diluted in the same buffer and incubated for 1 hour at room temperature.

Article Title: Analyses of astrocyte-neuron lactate shuttle transporter levels in brain tissues from people with HIV-associated neurocognitive impairment and Alzheimer’s disease
Article Snippet: After the transfer, total protein was imaged using a Bio-Rad ChemiDoc imager under the stain-free blot setting to control the transfer quality. .. The membranes were then blocked with 1 × Tris Buffered Saline with 1 % Casein (Bio-Rad, Cat. no: 1610782) for 1 h before incubation overnight at 4 ◦C with the primary antibody (Table 3). .. Anti-rabbit IgG, HRP-linked antibody was used as the secondary antibody for 1 h at room temperature (Bio-Rad, cat. no. 170–6516; 1:5000 in Phosphatebuffered saline (PBS)-Twin), visualized with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, Cat. no. 34095).

Western Blot:

other:

Article Title: Suppression of astrocyte BMP signaling improves molecular signatures and functional deficits in a fragile X syndrome mouse model.
Article Snippet: Proteins were transferred onto Immobilion-FL PVDF membranes (Millipore IPFL00010) at 100V for 60 min for acute astrocytes, or onto Amersham Hybond 0.2 μm PVDF membranes (Cytiva 10-6000-22) at 80V for 120 min in a solution of 10% Tris-Glycine Buffer (Thermo Fisher Scientific 28363).

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