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SP-5020  (vector laboratories)


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    Structured Review

    vector laboratories SP-5020
    Sp 5020, supplied by vector laboratories, used in various techniques. Bioz Stars score: 95/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/casein+solution/10x+Casein+Solution/pmc13080397-47-0-5
    Average 95 stars, based on 227 article reviews
    SP-5020 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title:
    Article Snippet: 10X Casein (250 mL) , Vector Laboratories , Cat# SP-5020.

    Blocking Assay:

    Article Title: Neoadjuvant Antiandrogen Therapy With or Without MEK or SRC Inhibition for Unfavorable-risk Prostate Cancer: A Phase 2 Randomized Clinical Trial.
    Article Snippet: University of CaliforniaLos Angeles, Los Angeles, CA, USA; Cedars-Sinai Medical Center, Department of Pathology and Laboratory Medicine, Los Angeles, CA, USA; i Department of Biostatistics, UCLA Fielding School of Public Health, University of California, Los Angeles, Los Angeles, CA, USA; Department of Medicine, Anthony Sisk c,g , David A. Elashoff i,j , Paul C. Boutros *, Matthew B. Rettig c,d,k,l ,

    Article Title: The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic dissemination
    Article Snippet: .. Target retrieval was performed in citrate buffer (pH 6; Vector Laboratories H-3300-250) or EDTA buffer (pH 9; Vector Laboratories H-3301-250), and blocking was done with casein-based buffer (Vector Laboratories SP-5020). .. After primary antibody, ImmPRESS HRP Polymer secondary antibody (Vector Laboratories; anti-mouse VECTMP745250 or anti-rabbit VECTMP740150) was added.

    Article Title: Generation and characterization of a novel inducible Sost_P2A_CreERT2 mouse model with high specificity for osteocytes
    Article Snippet: .. Sections were then incubated in blocking buffer comprised of 1 mL 10X Casein Solution (Vector), 9 mL 1× PBS, and 20 μL Triton-X (Fisher Scientific) at room temp for 1 h. Sections were rinsed twice with 1× PBS. .. Sclerostin antibody (AF1589, R&D Systems) was diluted 1:200 in Primary Ab buffer (10 mL 1× PBS, 100 mg BSA, and 20 μL Triton-X).

    Article Title: The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic dissemination.
    Article Snippet: .. Target retrieval was performed in Citrate buffer (Ph 6, Vector Laboratories #H-3300-250) or EDTA Buffer (Ph 9, Vector Laboratories #H-3301-250) and blocking was done with casein-based buffer (Vector Laboratories, 25 #SP-5020). .. After primary antibody, ImmPRESS HRP Polymer secondary antibodies (Vector Laboratories; anti-mouse VECTMP745250 or anti-rabbit VECTMP740150) was added.

    Incubation:

    Article Title: Generation and characterization of a novel inducible Sost_P2A_CreERT2 mouse model with high specificity for osteocytes
    Article Snippet: .. Sections were then incubated in blocking buffer comprised of 1 mL 10X Casein Solution (Vector), 9 mL 1× PBS, and 20 μL Triton-X (Fisher Scientific) at room temp for 1 h. Sections were rinsed twice with 1× PBS. .. Sclerostin antibody (AF1589, R&D Systems) was diluted 1:200 in Primary Ab buffer (10 mL 1× PBS, 100 mg BSA, and 20 μL Triton-X).

    Article Title: Injury-induced KIF4A neural expression and its role in Schwann cell proliferation suggest a dual function for this kinesin in neural regeneration
    Article Snippet: Fixed samples from human nerve tissue and rat peripheral nerves, DRG and spinal cord tissues were either i) embedded in Tissue-Tek® optimal cutting temperature (OCT) cryo-compound (Sakura, Finetek, Torrance, CA, USA), sectioned into 10 μm tissue cryosections, subsequently thaw mounted and stored at –20°C until further use; or ii) dehydrated in a graded series of ethanol (70%–100%), cleared in xylol, paraffin-embedded and sectioned into 5 μm tissue sections. .. After OCT washing or paraffin washing and rehydration, cryosections were blocked with 10% donkey serum (Sigma-Aldrich, Burlington, MA, USA) and paraffin-embedded sections were incubated with antigen retrieval buffers (10 mM sodium citrate, 0.05% Tween 20, pH 6; or 1 mM EDTA, pH 8), treated with H 2 O 2 to quench endogenous peroxidase (in case of HRP-conjugated secondary antibodies) and blocked with 2.5% horse serum (Vector Laboratories, Burlingame, CA, USA) and 1× casein solution (Vector Laboratories). ..



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