casein solution (Vector Laboratories)

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Vector Laboratories casein solution
Casein Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casein+solution/10x+Casein+Solution/pmc12410863-118-72-74
Average 95 stars, based on 233 article reviews

casein solution - by Bioz Stars, 2026-11

95/100 stars

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Article Title:
Article Snippet: 10X Casein (250 mL) , Vector Laboratories , Cat# SP-5020.

Blocking Assay:

Article Title: The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic dissemination
Article Snippet: .. Target retrieval was performed in citrate buffer (pH 6; Vector Laboratories H-3300-250) or EDTA buffer (pH 9; Vector Laboratories H-3301-250), and blocking was done with casein-based buffer (Vector Laboratories SP-5020). .. After primary antibody, ImmPRESS HRP Polymer secondary antibody (Vector Laboratories; anti-mouse VECTMP745250 or anti-rabbit VECTMP740150) was added.

Article Title: Generation and characterization of a novel inducible Sost_P2A_CreERT2 mouse model with high specificity for osteocytes
Article Snippet: .. Sections were then incubated in blocking buffer comprised of 1 mL 10X Casein Solution (Vector), 9 mL 1× PBS, and 20 μL Triton-X (Fisher Scientific) at room temp for 1 h. Sections were rinsed twice with 1× PBS. .. Sections were then incubated in blocking buffer comprised of 1 mL 10X Casein Solution (Vector), 9 mL 1× PBS, and 20 μL Triton-X (Fisher Scientific) at room temp for 1 h. Sections were rinsed twice with 1× PBS.

Article Title: The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic dissemination.
Article Snippet: .. Target retrieval was performed in Citrate buffer (Ph 6, Vector Laboratories #H-3300-250) or EDTA Buffer (Ph 9, Vector Laboratories #H-3301-250) and blocking was done with casein-based buffer (Vector Laboratories, 25 #SP-5020). .. After primary antibody, ImmPRESS HRP Polymer secondary antibodies (Vector Laboratories; anti-mouse VECTMP745250 or anti-rabbit VECTMP740150) was added.

Incubation:

Article Title: Complementary PET and MRI of myeloperoxidase activity as a noninvasive surrogate for tissue acrolein in experimental neuroinflammation
Article Snippet: Duplicate gels were stained with Coomassie blue (ThermoScientific, 20278) for normalization. .. Proteins were transferred to nitrocellulose membrane using Power Blotter-Semi-dry Transfer System (Thermo Fisher Scientific) which was blocked with 1x casein (Vector, #SP-5020) for 30 min, incubated overnight (4 °C) with anti-acrolein-lysine antibody (StressMarq, SMC-505, 1:1000), washed with 0.1% TBST, and incubated with secondary antibodies (Vector, #BA-2000, 1:2000) for 45 min. .. Following TBST washing, the membrane was treated with VECTASTAIN ABC-AmP reagent (Vector Laboratories) for 10 min. DuoLuX substrate (Vector, #SK-6605) was used for chemiluminescent detection on an Azure C300 Western blot imaging system (Azure Biosystems).

Article Title: Injury-induced KIF4A neural expression and its role in Schwann cell proliferation suggest a dual function for this kinesin in neural regeneration
Article Snippet: Fixed samples from human nerve tissue and rat peripheral nerves, DRG and spinal cord tissues were either i) embedded in Tissue-Tek optimal cutting temperature (OCT) cryo-compound (Sakura, Finetek, Torrance, CA, USA), sectioned into 10 μm tissue cryosections, subsequently thaw mounted and stored at –20°C until further use; or ii) dehydrated in a graded series of ethanol (70%–100%), cleared in xylol, paraffin-embedded and sectioned into 5 μm tissue sections. .. After OCT washing or paraffin washing and rehydration, cryosections were blocked with 10% donkey serum (Sigma-Aldrich, Burlington, MA, USA) and paraffin-embedded sections were incubated with antigen retrieval buffers (10 mM sodium citrate, 0.05% Tween 20, pH 6; or 1 mM EDTA, pH 8), treated with H 2 O 2 to quench endogenous peroxidase (in case of HRP-conjugated secondary antibodies) and blocked with 2.5% horse serum (Vector Laboratories, Burlingame, CA, USA) and 1× casein solution (Vector Laboratories). ..

Membrane:

Tissue:

Article Title: In Vivo Evaluation of a Multilayered, Fibrin-Agarose Urethral Substitute Generated by Tissue Engineering
Article Snippet: .. In brief, tissue sections were subjected to antigen retrieval with ethylenediaminetetraacetic acid (EDTA) (AGEL3554, Bionova, Madrid, Spain), citrate (MAD004071R, Vitro) or Tris-EDTA (MAD-004070R, Vitro) buffer at 98 °C (for 25 min) or with chondroitinase ABC (C3667, Merck), endogenous peroxidase was quenched for 10 min in 1:9 hydrogen peroxide (131077.1211, Panreac) in PBS, and samples were preincubated for 30 min in normal horse serum (S-2012, Vector Labs, Newark, CA, USA) and casein (SP-5020, Vector Labs). .. Primary antibodies were applied overnight at 4 °C, and a ready-to-use secondary antibody mixture conjugated with peroxidase (MP-7401-50, Vector Labs) was used for 1h at room temperature.

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