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pbs hsp70 cas9  (Addgene inc)


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    Structured Review

    Addgene inc pbs hsp70 cas9
    Pbs Hsp70 Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+coding+sequence/pBS-Hsp70-Cas9+(Plasmid+%2346294)/pmc08555087-626-21-22
    Average 93 stars, based on 41 article reviews
    pbs hsp70 cas9 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: The Drosophila ZAD zinc finger protein Mulberry shapes the organization of the regulatory genome in the early embryo
    Article Snippet: .. A pair of pCFD3 gRNA expression plasmids, pBS-hsp70-Cas9 plasmid (addgene #46294) and pBS-loxP-3×P3-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were co-injected using yw strain. ..

    Article Title: The Drosophila ZAD zinc finger protein Mulberry shapes the organization of the regulatory genome in the early embryo
    Article Snippet: .. To generate CG31365-GFP-3×FLAG strain, a gRNA was designed to target PAM sequence just upstream of the stop codon. pCFD3 gRNA expression plasmid, pBS-hsp70-Cas9 plasmid (addgene #46294) and pBS-GFP-3×FLAG-loxP-3×P3-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were co-injected using yw strain. .. A pair of pCFD3 gRNA expression plasmids, pBS-hsp70-Cas9 plasmid (addgene #46294) and pBS-loxP-3×P3-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were co-injected using yw strain.

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc-finger proteins in Drosophila
    Article Snippet: .. For GFP-3xFLAG/mCherry-3xFLAG insertion, gRNA was designed to target PAM-containing sequence adjacent to the stop codon. pCFD3 gRNA expression plasmid, pBS-Hsp70-Cas9 plasmid (addgene# 46294) and pBS-GFP-3xFLAG/mCherry-3xFLAG-dsRed-loxP donor plasmid containing 5’ and 3’ homology arms were co-injected using the standard yw laboratory strain. ..

    Article Title: Transient residence of the repulsive client Shutdown in Yb bodies plays a critical role in Piwi-piRISC biogenesis and maintaining fertility.
    Article Snippet: Article Transient residence of the repulsive client Shutdown in Yb bodies plays a critical role in Piwi-piRISC biogenesis and maintaining fertility

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Drosophila
    Article Snippet: .. For GFP-3xFLAG/mCherry-3xFLAG insertion, guide RNA (gRNA) was designed to target protospacer adjacent motif (PAM)–containing sequence adjacent to the stop codon. pCFD3 gRNA expression plasmid, pBS-Hsp70-Cas9 plasmid (Addgene, #46294), and pBS-GFP-3xFLAG/mCherry-3xFLAG-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were coinjected using the standard yw laboratory strain. ..

    Plasmid Preparation:

    Article Title: The Drosophila ZAD zinc finger protein Mulberry shapes the organization of the regulatory genome in the early embryo
    Article Snippet: .. A pair of pCFD3 gRNA expression plasmids, pBS-hsp70-Cas9 plasmid (addgene #46294) and pBS-loxP-3×P3-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were co-injected using yw strain. ..

    Article Title: The Drosophila ZAD zinc finger protein Mulberry shapes the organization of the regulatory genome in the early embryo
    Article Snippet: .. To generate CG31365-GFP-3×FLAG strain, a gRNA was designed to target PAM sequence just upstream of the stop codon. pCFD3 gRNA expression plasmid, pBS-hsp70-Cas9 plasmid (addgene #46294) and pBS-GFP-3×FLAG-loxP-3×P3-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were co-injected using yw strain. .. A pair of pCFD3 gRNA expression plasmids, pBS-hsp70-Cas9 plasmid (addgene #46294) and pBS-loxP-3×P3-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were co-injected using yw strain.

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc-finger proteins in Drosophila
    Article Snippet: .. For GFP-3xFLAG/mCherry-3xFLAG insertion, gRNA was designed to target PAM-containing sequence adjacent to the stop codon. pCFD3 gRNA expression plasmid, pBS-Hsp70-Cas9 plasmid (addgene# 46294) and pBS-GFP-3xFLAG/mCherry-3xFLAG-dsRed-loxP donor plasmid containing 5’ and 3’ homology arms were co-injected using the standard yw laboratory strain. ..

    Article Title: Transient residence of the repulsive client Shutdown in Yb bodies plays a critical role in Piwi-piRISC biogenesis and maintaining fertility.
    Article Snippet: Article Transient residence of the repulsive client Shutdown in Yb bodies plays a critical role in Piwi-piRISC biogenesis and maintaining fertility

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Drosophila
    Article Snippet: .. For GFP-3xFLAG/mCherry-3xFLAG insertion, guide RNA (gRNA) was designed to target protospacer adjacent motif (PAM)–containing sequence adjacent to the stop codon. pCFD3 gRNA expression plasmid, pBS-Hsp70-Cas9 plasmid (Addgene, #46294), and pBS-GFP-3xFLAG/mCherry-3xFLAG-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were coinjected using the standard yw laboratory strain. ..

    Sequencing:

    Article Title: The Drosophila ZAD zinc finger protein Mulberry shapes the organization of the regulatory genome in the early embryo
    Article Snippet: .. To generate CG31365-GFP-3×FLAG strain, a gRNA was designed to target PAM sequence just upstream of the stop codon. pCFD3 gRNA expression plasmid, pBS-hsp70-Cas9 plasmid (addgene #46294) and pBS-GFP-3×FLAG-loxP-3×P3-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were co-injected using yw strain. .. A pair of pCFD3 gRNA expression plasmids, pBS-hsp70-Cas9 plasmid (addgene #46294) and pBS-loxP-3×P3-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were co-injected using yw strain.

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc-finger proteins in Drosophila
    Article Snippet: .. For GFP-3xFLAG/mCherry-3xFLAG insertion, gRNA was designed to target PAM-containing sequence adjacent to the stop codon. pCFD3 gRNA expression plasmid, pBS-Hsp70-Cas9 plasmid (addgene# 46294) and pBS-GFP-3xFLAG/mCherry-3xFLAG-dsRed-loxP donor plasmid containing 5’ and 3’ homology arms were co-injected using the standard yw laboratory strain. ..

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Drosophila
    Article Snippet: .. For GFP-3xFLAG/mCherry-3xFLAG insertion, guide RNA (gRNA) was designed to target protospacer adjacent motif (PAM)–containing sequence adjacent to the stop codon. pCFD3 gRNA expression plasmid, pBS-Hsp70-Cas9 plasmid (Addgene, #46294), and pBS-GFP-3xFLAG/mCherry-3xFLAG-dsRed-loxP donor plasmid containing 5′ and 3′ homology arms were coinjected using the standard yw laboratory strain. ..

    other:

    Article Title: The Drosophila ZAD zinc finger protein Mulberry shapes the organization of the regulatory genome in the early embryo
    Article Snippet: Aligned embryos were dried with silica gel for ∼8 min and covered with FL-100-1000CS silicone oil (Shin-Etsu Silicone).



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    Image Search Results


    Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Modification, Expressing, Sequencing, Quantitative Proteomics, Quantitative RT-PCR, Comparison

    Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Comparison, Plasmid Preparation, Selection, Amplification, Sequencing