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p53 crispr cas9 ko plasmid  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology p53 crispr cas9 ko plasmid
    P53 Crispr Cas9 Ko Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+coding+sequence/p53+CRISPR%2FCas9+KO+Plasmid/pmc05510006-837-14-34
    Average 95 stars, based on 28 article reviews
    p53 crispr cas9 ko plasmid - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    shRNA:

    Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators
    Article Snippet: .. Moreover, mutilple siRNA, shRNA, CRISPR constructs for reducing TP53 expression can be found in the commercial product lists of the above-referenced companies, such as siRNA products #sc-29435 and sc-44218, and CRISPR product #sc-416469 from Santa Cruz Biotechnology, RNAi products SR322075 and TL320558V, and CRISPR product KN200003 (Origene), and multiple CRISPR products from GenScript (Piscataway, NJ). .. Chemical inhibitors of TP53 are also available, including, e.g., Cyclic Pifithrin-a hydrobromide, RITA (TOCRIS, MN).

    CRISPR:

    Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators
    Article Snippet: .. Moreover, mutilple siRNA, shRNA, CRISPR constructs for reducing TP53 expression can be found in the commercial product lists of the above-referenced companies, such as siRNA products #sc-29435 and sc-44218, and CRISPR product #sc-416469 from Santa Cruz Biotechnology, RNAi products SR322075 and TL320558V, and CRISPR product KN200003 (Origene), and multiple CRISPR products from GenScript (Piscataway, NJ). .. Chemical inhibitors of TP53 are also available, including, e.g., Cyclic Pifithrin-a hydrobromide, RITA (TOCRIS, MN).

    Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis.
    Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318). .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318).


    Article Title: NUAKs facilitate mTOR-mediated NSCLC proliferation and metastasis by modulating glucose metabolism and inhibiting p53 activity.
    Article Snippet: Non-small cell lung cancer (NSCLC) cells frequently exhibit aberrant glucose metabolism, characterized by elevated aerobic glycolysis, pentose phosphate pathway (PPP), and reduced oxidative phosphorylation.. However, the specific mechanisms underlying the abnormal activation of glucose metabolism and its contribution to NSCLC tumorigenesis remain incompletely elucidated.. In this study, we observed that both NUAK1 and NUAK2 mRNA expression levels were significantly elevated in NSCLC tissues compared to non-tumor tissues, and that high NUAK1/2 expression correlated with poor prognosis in NSCLC patients.

    Article Title: CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2
    Article Snippet: pSpCas9-2A-GFP-CEP192 Exon11_3’_Guide 2 , This study. gRNA sequence in Appendix Table S . , Original plasmid from addgene Cat# 48138.. .. p53 CRISPR/Cas9 KO Plasmid , SantaCruz Biotechnology , Cat #sc-4146469. ..

    Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis
    Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318). ..

    Construct:

    Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators
    Article Snippet: .. Moreover, mutilple siRNA, shRNA, CRISPR constructs for reducing TP53 expression can be found in the commercial product lists of the above-referenced companies, such as siRNA products #sc-29435 and sc-44218, and CRISPR product #sc-416469 from Santa Cruz Biotechnology, RNAi products SR322075 and TL320558V, and CRISPR product KN200003 (Origene), and multiple CRISPR products from GenScript (Piscataway, NJ). .. Chemical inhibitors of TP53 are also available, including, e.g., Cyclic Pifithrin-a hydrobromide, RITA (TOCRIS, MN).

    Expressing:

    Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators
    Article Snippet: .. Moreover, mutilple siRNA, shRNA, CRISPR constructs for reducing TP53 expression can be found in the commercial product lists of the above-referenced companies, such as siRNA products #sc-29435 and sc-44218, and CRISPR product #sc-416469 from Santa Cruz Biotechnology, RNAi products SR322075 and TL320558V, and CRISPR product KN200003 (Origene), and multiple CRISPR products from GenScript (Piscataway, NJ). .. Chemical inhibitors of TP53 are also available, including, e.g., Cyclic Pifithrin-a hydrobromide, RITA (TOCRIS, MN).

    Knock-Out:

    Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis.
    Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318). .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318).

    Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis
    Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318). ..

    Plasmid Preparation:

    Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis.
    Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318). .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318).


    Article Title: NUAKs facilitate mTOR-mediated NSCLC proliferation and metastasis by modulating glucose metabolism and inhibiting p53 activity.
    Article Snippet: Non-small cell lung cancer (NSCLC) cells frequently exhibit aberrant glucose metabolism, characterized by elevated aerobic glycolysis, pentose phosphate pathway (PPP), and reduced oxidative phosphorylation.. However, the specific mechanisms underlying the abnormal activation of glucose metabolism and its contribution to NSCLC tumorigenesis remain incompletely elucidated.. In this study, we observed that both NUAK1 and NUAK2 mRNA expression levels were significantly elevated in NSCLC tissues compared to non-tumor tissues, and that high NUAK1/2 expression correlated with poor prognosis in NSCLC patients.

    Article Title: CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2
    Article Snippet: pSpCas9-2A-GFP-CEP192 Exon11_3’_Guide 2 , This study. gRNA sequence in Appendix Table S . , Original plasmid from addgene Cat# 48138.. .. p53 CRISPR/Cas9 KO Plasmid , SantaCruz Biotechnology , Cat #sc-4146469. ..

    Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis
    Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the p53 plasmid (#sc-437318). ..

    Transfection:


    other:

    Article Title: CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2.
    Article Snippet: Reagent/Resource Reference or source Identifier or catalogue number Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (1:1000) Thermo Scientific Cat #A-21206, RRID: AB_2535792 Oligonucleotides and other sequence-based reagents PCR primers This study Table S4 siBORA (Silencer Select) Thermo Scientific Cat #s36521 siCEP192 (Silencer Select) Thermo Scientific Cat #s226819 siTACC3_1 (Silencer Select) Thermo Scientific Cat #s20470 siTACC3_2 (Silencer Select) Thermo Scientific Cat #s20471 siTPX2 (Silencer Select) Thermo Scientific Cat #s22747 siNegative Control No.1 (siControl) (Silencer Select) Thermo Scientific Cat #4390843 Chemicals, Enzymes and other reagents Sodium chloride Fisher Cat #BP358-212 Tris Melford Cat #T600-40 TCEP Fluorochem Cat #M02624 Tween 20 Millipore Cat #655204 Magnesium chloride hexahydrate Sigma Cat #63068 Glycerol Fisher Cat #G/0650/17 cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail Roche Cat #11836170001 Hepes Sigma Cat #H4034 DTT Melford Cat #D11000 TBS Thermo Scientific Cat #J62938K7 Kanamycin Sigma Cat #K4000 Spectinomycin Thermo Scientific Cat #J61810.14 Chloramphenicol Sigma Cat #C0378 Imidazole Thermo Scientific Cat #122025000 IPTG Protein Arc Cat #GEN-S-02122 FAM-TPX2 7-43 This study.



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    Fig. 2 The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001
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    Fig. 2 The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001
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    Image Search Results


    Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Modification, Expressing, Sequencing, Quantitative Proteomics, Quantitative RT-PCR, Comparison

    Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Comparison, Plasmid Preparation, Selection, Amplification, Sequencing