p53 crispr cas9 ko plasmid (Santa Cruz Biotechnology)
Structured Review
P53 Crispr Cas9 Ko Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+coding+sequence/p53+CRISPR%2FCas9+KO+Plasmid/pmc05510006-837-14-34
Average 95 stars, based on 28 article reviews
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shRNA:Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators Article Snippet: .. Moreover, mutilple siRNA, shRNA, CRISPR constructs for reducing TP53 expression can be found in the commercial product lists of the above-referenced companies, such as siRNA products #sc-29435 and sc-44218, and CRISPR:Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators Article Snippet: .. Moreover, mutilple siRNA, shRNA, CRISPR constructs for reducing TP53 expression can be found in the commercial product lists of the above-referenced companies, such as siRNA products #sc-29435 and sc-44218, and Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis. Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the Article Title: Commensal papillomavirus immunity preserves the homeostasis of highly mutated normal skin. Article Snippet: Article Article Title: NUAKs facilitate mTOR-mediated NSCLC proliferation and metastasis by modulating glucose metabolism and inhibiting p53 activity. Article Snippet: Non-small cell lung cancer (NSCLC) cells frequently exhibit aberrant glucose metabolism, characterized by elevated aerobic glycolysis, pentose phosphate pathway (PPP), and reduced oxidative phosphorylation.. However, the specific mechanisms underlying the abnormal activation of glucose metabolism and its contribution to NSCLC tumorigenesis remain incompletely elucidated.. In this study, we observed that both NUAK1 and NUAK2 mRNA expression levels were significantly elevated in NSCLC tissues compared to non-tumor tissues, and that high NUAK1/2 expression correlated with poor prognosis in NSCLC patients. Article Title: CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2 Article Snippet: pSpCas9-2A-GFP-CEP192 Exon11_3’_Guide 2 , This study. gRNA sequence in Appendix Table S . , Original plasmid from addgene Cat# 48138.. .. Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the Construct:Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators Article Snippet: .. Moreover, mutilple siRNA, shRNA, CRISPR constructs for reducing TP53 expression can be found in the commercial product lists of the above-referenced companies, such as siRNA products #sc-29435 and sc-44218, and Expressing:Article Title: Compositions and methods for identification assessment, prevention, and treatment of Ewing sarcoma using TP53 dependency biomarkers and modulators Article Snippet: .. Moreover, mutilple siRNA, shRNA, CRISPR constructs for reducing TP53 expression can be found in the commercial product lists of the above-referenced companies, such as siRNA products #sc-29435 and sc-44218, and Knock-Out:Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis. Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the Plasmid Preparation:Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis. Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the Article Title: Commensal papillomavirus immunity preserves the homeostasis of highly mutated normal skin. Article Snippet: Article Article Title: NUAKs facilitate mTOR-mediated NSCLC proliferation and metastasis by modulating glucose metabolism and inhibiting p53 activity. Article Snippet: Non-small cell lung cancer (NSCLC) cells frequently exhibit aberrant glucose metabolism, characterized by elevated aerobic glycolysis, pentose phosphate pathway (PPP), and reduced oxidative phosphorylation.. However, the specific mechanisms underlying the abnormal activation of glucose metabolism and its contribution to NSCLC tumorigenesis remain incompletely elucidated.. In this study, we observed that both NUAK1 and NUAK2 mRNA expression levels were significantly elevated in NSCLC tissues compared to non-tumor tissues, and that high NUAK1/2 expression correlated with poor prognosis in NSCLC patients. Article Title: CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2 Article Snippet: pSpCas9-2A-GFP-CEP192 Exon11_3’_Guide 2 , This study. gRNA sequence in Appendix Table S . , Original plasmid from addgene Cat# 48138.. .. Article Title: MYLK4 promotes colorectal cancer progression by regulating lipid metabolism reprogramming via targeting ferroptosis Article Snippet: .. Santa Cruz Biotechnology supplied the pre-assembled CRISPR/Cas9 knockout products for the human PDRG1 plasmid (#sc-410621) and the Transfection:Article Title: Commensal papillomavirus immunity preserves the homeostasis of highly mutated normal skin. Article Snippet: Article other:Article Title: CEP192 localises mitotic Aurora-A activity by priming its interaction with TPX2. Article Snippet: Reagent/Resource Reference or source Identifier or catalogue number Donkey anti-Rabbit IgG (H+ L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (1:1000) Thermo Scientific Cat #A-21206, RRID: AB_2535792 Oligonucleotides and other sequence-based reagents PCR primers This study Table S4 siBORA (Silencer Select) Thermo Scientific Cat #s36521 siCEP192 (Silencer Select) Thermo Scientific Cat #s226819 siTACC3_1 (Silencer Select) Thermo Scientific Cat #s20470 siTACC3_2 (Silencer Select) Thermo Scientific Cat #s20471 siTPX2 (Silencer Select) Thermo Scientific Cat #s22747 siNegative Control No.1 (siControl) (Silencer Select) Thermo Scientific Cat #4390843 Chemicals, Enzymes and other reagents Sodium chloride Fisher Cat #BP358-212 Tris Melford Cat #T600-40 TCEP Fluorochem Cat #M02624 Tween 20 Millipore Cat #655204 Magnesium chloride hexahydrate Sigma Cat #63068 Glycerol Fisher Cat #G/0650/17 cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail Roche Cat #11836170001 Hepes Sigma Cat #H4034 DTT Melford Cat #D11000 TBS Thermo Scientific Cat #J62938K7 Kanamycin Sigma Cat #K4000 Spectinomycin Thermo Scientific Cat #J61810.14 Chloramphenicol Sigma Cat #C0378 Imidazole Thermo Scientific Cat #122025000 IPTG Protein Arc Cat #GEN-S-02122 FAM-TPX2 7-43 This study. |
