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vector carrying ace2 coding sequences  (Addgene inc)


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    Addgene inc vector carrying ace2 coding sequences
    Vector Carrying Ace2 Coding Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+coding+sequence/ACE2+g1+%2B+Cas9+(Plasmid+%23153011)/pm40133950-166-13-23
    Average 91 stars, based on 1 article reviews
    vector carrying ace2 coding sequences - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Generated:

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication.
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein.
    Article Snippet: .. A549 cells stably co-expressing ACE2 and TMPRSS2 (A549-A/T) were generated through sequential transduction of A549 cells with TMPRSS2-encoding (generated using Addgene plasmid #170390, a gift from Nir Hacohen) and ACE2-encoding (generated using Addgene PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1010811 September 12, 2022 20 / 30 plasmid #154981, a gift from Sonja Best) lentiviruses and selection with hygromycin (250 μg/ mL) and blasticidin (20 μg/mL) for 10 days, respectively. ..

    Transfection:

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication.
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: In-Vitro Fluorescence Microscopy Studies Show Retention of Spike-Protein (SARS-Cov-2) on Cell Membrane in the Presence of Amodiaquin Dihydrochloride Dihydrate Drug
    Article Snippet: .. Hence, the cells were first transfected with an ACE2 plasmid (name, “pcDNA3.1-hACE2” from AddGene) in order to see binding of the RBD at the surface. ..

    Fluorescence:

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication.
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Marker:

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication.
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG-Syt1-SV2 plasma membrane nanocluster for synaptic vesicle entry.
    Article Snippet: .. All sgRNAs for rat Syt1 wildtype and K52A were constructed on the same backbone vector (#AAAA-0244, termed “dCas9-KRAB-TagBFP2”; Table EV3) by DNA Dream Lab. We used the pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro construct (dCAS9KRAB; a gift from Charles Gersbach, Addgene plasmid #71236; http://n2t.net/addgene:71236; RRID: Addgene_71236 (Thakore et al, 2015)), where the puromycin-resistance gene was replaced by the TagBFP2 (tag blue fluorescent protein 2) marker, which was PCR-amplified from the ACE2 g1+ Cas9 plasmid (a gift from Jason Sheltzer, Addgene plasmid #153011; http://n2t.net/addgene: 153011; RRID: Addgene_153011). .. The replacement was made with the NEBuilder kit (NEB, Cat. #E2621S) by concatenating the PCRlinearized dCAS9-KRAB construct and TagBFP2 fluorescent marker with the primers indicated in Table EV2.

    Article Title: Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG‐Syt1 ‐ SV2 plasma membrane nanocluster for synaptic vesicle entry
    Article Snippet: .. All sgRNAs for rat Syt1 wildtype and K52A were constructed on the same backbone vector (#AAAA‐0244, termed “dCas9‐KRAB‐TagBFP2”; Table ) by DNA Dream Lab. We used the pLV hU6‐sgRNA hUbC‐dCas9‐KRAB‐T2a‐Puro construct (dCAS9‐KRAB; a gift from Charles Gersbach, Addgene plasmid #71236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236 (Thakore et al , )), where the puromycin‐resistance gene was replaced by the TagBFP2 (tag blue fluorescent protein 2) marker, which was PCR‐amplified from the ACE2 g1 + Cas9 plasmid (a gift from Jason Sheltzer, Addgene plasmid #153011; http://n2t.net/addgene:153011 ; RRID: Addgene_153011). .. The replacement was made with the NEBuilder kit (NEB, Cat. #E2621S) by concatenating the PCR‐linearized dCAS9‐KRAB construct and TagBFP2 fluorescent marker with the primers indicated in Table .

    Plasmid Preparation:

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication.
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication
    Article Snippet: .. TBLU cells overexpressing the human ACE2 ortholog was generated by transfection of a vector carrying ACE2 coding sequences and the blue fluorescence marker (Addgene plasmid no. 164219) into TBLU cells through Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: In-Vitro Fluorescence Microscopy Studies Show Retention of Spike-Protein (SARS-Cov-2) on Cell Membrane in the Presence of Amodiaquin Dihydrochloride Dihydrate Drug
    Article Snippet: .. Hence, the cells were first transfected with an ACE2 plasmid (name, “pcDNA3.1-hACE2” from AddGene) in order to see binding of the RBD at the surface. ..

    Article Title: SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein.
    Article Snippet: .. A549 cells stably co-expressing ACE2 and TMPRSS2 (A549-A/T) were generated through sequential transduction of A549 cells with TMPRSS2-encoding (generated using Addgene plasmid #170390, a gift from Nir Hacohen) and ACE2-encoding (generated using Addgene PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1010811 September 12, 2022 20 / 30 plasmid #154981, a gift from Sonja Best) lentiviruses and selection with hygromycin (250 μg/ mL) and blasticidin (20 μg/mL) for 10 days, respectively. ..

    Article Title: Novel Trispecific Neutralizing Antibodies With Enhanced Potency and Breadth Against Pan‐Sarbecoviruses
    Article Snippet: .. The mammalian expression plasmid encoding recombinant soluble dimeric ACE2 was obtained from Addgene. .. Expi293 cells (Thermo Fisher Scientific) were transiently transfected with the corresponding expression plasmids using PEI.

    Article Title: Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG-Syt1-SV2 plasma membrane nanocluster for synaptic vesicle entry.
    Article Snippet: .. All sgRNAs for rat Syt1 wildtype and K52A were constructed on the same backbone vector (#AAAA-0244, termed “dCas9-KRAB-TagBFP2”; Table EV3) by DNA Dream Lab. We used the pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro construct (dCAS9KRAB; a gift from Charles Gersbach, Addgene plasmid #71236; http://n2t.net/addgene:71236; RRID: Addgene_71236 (Thakore et al, 2015)), where the puromycin-resistance gene was replaced by the TagBFP2 (tag blue fluorescent protein 2) marker, which was PCR-amplified from the ACE2 g1+ Cas9 plasmid (a gift from Jason Sheltzer, Addgene plasmid #153011; http://n2t.net/addgene: 153011; RRID: Addgene_153011). .. The replacement was made with the NEBuilder kit (NEB, Cat. #E2621S) by concatenating the PCRlinearized dCAS9-KRAB construct and TagBFP2 fluorescent marker with the primers indicated in Table EV2.

    Article Title: Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG‐Syt1 ‐ SV2 plasma membrane nanocluster for synaptic vesicle entry
    Article Snippet: .. All sgRNAs for rat Syt1 wildtype and K52A were constructed on the same backbone vector (#AAAA‐0244, termed “dCas9‐KRAB‐TagBFP2”; Table ) by DNA Dream Lab. We used the pLV hU6‐sgRNA hUbC‐dCas9‐KRAB‐T2a‐Puro construct (dCAS9‐KRAB; a gift from Charles Gersbach, Addgene plasmid #71236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236 (Thakore et al , )), where the puromycin‐resistance gene was replaced by the TagBFP2 (tag blue fluorescent protein 2) marker, which was PCR‐amplified from the ACE2 g1 + Cas9 plasmid (a gift from Jason Sheltzer, Addgene plasmid #153011; http://n2t.net/addgene:153011 ; RRID: Addgene_153011). .. The replacement was made with the NEBuilder kit (NEB, Cat. #E2621S) by concatenating the PCR‐linearized dCAS9‐KRAB construct and TagBFP2 fluorescent marker with the primers indicated in Table .

    Binding Assay:

    Article Title: In-Vitro Fluorescence Microscopy Studies Show Retention of Spike-Protein (SARS-Cov-2) on Cell Membrane in the Presence of Amodiaquin Dihydrochloride Dihydrate Drug
    Article Snippet: .. Hence, the cells were first transfected with an ACE2 plasmid (name, “pcDNA3.1-hACE2” from AddGene) in order to see binding of the RBD at the surface. ..

    Stable Transfection:

    Article Title: SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein.
    Article Snippet: .. A549 cells stably co-expressing ACE2 and TMPRSS2 (A549-A/T) were generated through sequential transduction of A549 cells with TMPRSS2-encoding (generated using Addgene plasmid #170390, a gift from Nir Hacohen) and ACE2-encoding (generated using Addgene PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1010811 September 12, 2022 20 / 30 plasmid #154981, a gift from Sonja Best) lentiviruses and selection with hygromycin (250 μg/ mL) and blasticidin (20 μg/mL) for 10 days, respectively. ..

    Transduction:

    Article Title: SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein.
    Article Snippet: .. A549 cells stably co-expressing ACE2 and TMPRSS2 (A549-A/T) were generated through sequential transduction of A549 cells with TMPRSS2-encoding (generated using Addgene plasmid #170390, a gift from Nir Hacohen) and ACE2-encoding (generated using Addgene PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1010811 September 12, 2022 20 / 30 plasmid #154981, a gift from Sonja Best) lentiviruses and selection with hygromycin (250 μg/ mL) and blasticidin (20 μg/mL) for 10 days, respectively. ..

    Selection:

    Article Title: SIRT5 is a proviral factor that interacts with SARS-CoV-2 Nsp14 protein.
    Article Snippet: .. A549 cells stably co-expressing ACE2 and TMPRSS2 (A549-A/T) were generated through sequential transduction of A549 cells with TMPRSS2-encoding (generated using Addgene plasmid #170390, a gift from Nir Hacohen) and ACE2-encoding (generated using Addgene PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1010811 September 12, 2022 20 / 30 plasmid #154981, a gift from Sonja Best) lentiviruses and selection with hygromycin (250 μg/ mL) and blasticidin (20 μg/mL) for 10 days, respectively. ..

    Expressing:

    Article Title: Novel Trispecific Neutralizing Antibodies With Enhanced Potency and Breadth Against Pan‐Sarbecoviruses
    Article Snippet: .. The mammalian expression plasmid encoding recombinant soluble dimeric ACE2 was obtained from Addgene. .. Expi293 cells (Thermo Fisher Scientific) were transiently transfected with the corresponding expression plasmids using PEI.

    Recombinant:

    Article Title: Novel Trispecific Neutralizing Antibodies With Enhanced Potency and Breadth Against Pan‐Sarbecoviruses
    Article Snippet: .. The mammalian expression plasmid encoding recombinant soluble dimeric ACE2 was obtained from Addgene. .. Expi293 cells (Thermo Fisher Scientific) were transiently transfected with the corresponding expression plasmids using PEI.

    Construct:

    Article Title: Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG-Syt1-SV2 plasma membrane nanocluster for synaptic vesicle entry.
    Article Snippet: .. All sgRNAs for rat Syt1 wildtype and K52A were constructed on the same backbone vector (#AAAA-0244, termed “dCas9-KRAB-TagBFP2”; Table EV3) by DNA Dream Lab. We used the pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro construct (dCAS9KRAB; a gift from Charles Gersbach, Addgene plasmid #71236; http://n2t.net/addgene:71236; RRID: Addgene_71236 (Thakore et al, 2015)), where the puromycin-resistance gene was replaced by the TagBFP2 (tag blue fluorescent protein 2) marker, which was PCR-amplified from the ACE2 g1+ Cas9 plasmid (a gift from Jason Sheltzer, Addgene plasmid #153011; http://n2t.net/addgene: 153011; RRID: Addgene_153011). .. The replacement was made with the NEBuilder kit (NEB, Cat. #E2621S) by concatenating the PCRlinearized dCAS9-KRAB construct and TagBFP2 fluorescent marker with the primers indicated in Table EV2.

    Article Title: Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG‐Syt1 ‐ SV2 plasma membrane nanocluster for synaptic vesicle entry
    Article Snippet: .. All sgRNAs for rat Syt1 wildtype and K52A were constructed on the same backbone vector (#AAAA‐0244, termed “dCas9‐KRAB‐TagBFP2”; Table ) by DNA Dream Lab. We used the pLV hU6‐sgRNA hUbC‐dCas9‐KRAB‐T2a‐Puro construct (dCAS9‐KRAB; a gift from Charles Gersbach, Addgene plasmid #71236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236 (Thakore et al , )), where the puromycin‐resistance gene was replaced by the TagBFP2 (tag blue fluorescent protein 2) marker, which was PCR‐amplified from the ACE2 g1 + Cas9 plasmid (a gift from Jason Sheltzer, Addgene plasmid #153011; http://n2t.net/addgene:153011 ; RRID: Addgene_153011). .. The replacement was made with the NEBuilder kit (NEB, Cat. #E2621S) by concatenating the PCR‐linearized dCAS9‐KRAB construct and TagBFP2 fluorescent marker with the primers indicated in Table .

    Polymerase Chain Reaction:

    Article Title: Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG-Syt1-SV2 plasma membrane nanocluster for synaptic vesicle entry.
    Article Snippet: .. All sgRNAs for rat Syt1 wildtype and K52A were constructed on the same backbone vector (#AAAA-0244, termed “dCas9-KRAB-TagBFP2”; Table EV3) by DNA Dream Lab. We used the pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro construct (dCAS9KRAB; a gift from Charles Gersbach, Addgene plasmid #71236; http://n2t.net/addgene:71236; RRID: Addgene_71236 (Thakore et al, 2015)), where the puromycin-resistance gene was replaced by the TagBFP2 (tag blue fluorescent protein 2) marker, which was PCR-amplified from the ACE2 g1+ Cas9 plasmid (a gift from Jason Sheltzer, Addgene plasmid #153011; http://n2t.net/addgene: 153011; RRID: Addgene_153011). .. The replacement was made with the NEBuilder kit (NEB, Cat. #E2621S) by concatenating the PCRlinearized dCAS9-KRAB construct and TagBFP2 fluorescent marker with the primers indicated in Table EV2.

    Article Title: Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG‐Syt1 ‐ SV2 plasma membrane nanocluster for synaptic vesicle entry
    Article Snippet: .. All sgRNAs for rat Syt1 wildtype and K52A were constructed on the same backbone vector (#AAAA‐0244, termed “dCas9‐KRAB‐TagBFP2”; Table ) by DNA Dream Lab. We used the pLV hU6‐sgRNA hUbC‐dCas9‐KRAB‐T2a‐Puro construct (dCAS9‐KRAB; a gift from Charles Gersbach, Addgene plasmid #71236; http://n2t.net/addgene:71236 ; RRID: Addgene_71236 (Thakore et al , )), where the puromycin‐resistance gene was replaced by the TagBFP2 (tag blue fluorescent protein 2) marker, which was PCR‐amplified from the ACE2 g1 + Cas9 plasmid (a gift from Jason Sheltzer, Addgene plasmid #153011; http://n2t.net/addgene:153011 ; RRID: Addgene_153011). .. The replacement was made with the NEBuilder kit (NEB, Cat. #E2621S) by concatenating the PCR‐linearized dCAS9‐KRAB construct and TagBFP2 fluorescent marker with the primers indicated in Table .



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    Image Search Results


    Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Modification, Expressing, Sequencing, Quantitative Proteomics, Quantitative RT-PCR, Comparison

    Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Comparison, Plasmid Preparation, Selection, Amplification, Sequencing