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mouse zbp1 coding sequence  (Addgene inc)


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    Addgene inc mouse zbp1 coding sequence
    Mouse Zbp1 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+coding+sequence/pCW-Cas9+(Plasmid+%2350661)/pm39300211-206-7-15
    Average 96 stars, based on 346 article reviews
    mouse zbp1 coding sequence - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: High-throughput ligand diversification to discover chemical inducers of proximity.
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated iCas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene, 50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs)122. ..

    Article Title: The Epigenetic Factor PHF13 Governs Trophoblast Stemness and Differentiation
    Article Snippet: .. Specifically, TS cells expressing doxycycline-inducible Cas9 and selected with puromycin were generated as previously reported , using pCW-Cas9 (Addgene, #50661) . .. For Cas9-mediated knockout, the gRNA sequence used (5’-ACTCATTACACTCGATCATG-3’) was cloned into a derivative of the plasmid (Addgene, #57825) modified to contain the blasticidin S deaminase resistance cassette.

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Generated:

    Article Title: High-throughput ligand diversification to discover chemical inducers of proximity.
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated iCas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene, 50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs)122. ..

    Article Title: The Epigenetic Factor PHF13 Governs Trophoblast Stemness and Differentiation
    Article Snippet: .. Specifically, TS cells expressing doxycycline-inducible Cas9 and selected with puromycin were generated as previously reported , using pCW-Cas9 (Addgene, #50661) . .. For Cas9-mediated knockout, the gRNA sequence used (5’-ACTCATTACACTCGATCATG-3’) was cloned into a derivative of the plasmid (Addgene, #57825) modified to contain the blasticidin S deaminase resistance cassette.

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Transduction:

    Article Title: High-throughput ligand diversification to discover chemical inducers of proximity.
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated iCas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene, 50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs)122. ..

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    Genome Wide:

    Article Title: High-throughput ligand diversification to discover chemical inducers of proximity.
    Article Snippet: .. Briefly, a NALM6 clone bearing an integrated iCas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene, 50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs)122. ..

    Article Title: CRISPR-based chemogenomic profiling reveals redox vulnerabilities to epigallocatechin-3-gallate and green tea polyphenol extract
    Article Snippet: .. Briefly, a human NALM-6 (pre-B ALL lymphocytes) clone bearing an integrated inducible Cas9 expression cassette generated by lentiviruses made from pCW-Cas9 (Addgene #50661) was transduced with the genome-wide KO EKO sgRNA library (278,754 different sgRNAs). ..

    CRISPR:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Knock-Out:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Construct:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Subcloning:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Plasmid Preparation:

    Article Title: LINE-1 Locus Transcription Nucleates Oncogenic Chromatin Architecture
    Article Snippet: The inducible CRISPR inhibition construct was generated by sub-cloning the 3xFLAG-dCas9-KRAB fragment from the pLV-hUbC-KRAB-dCas9-2A-PuroR vector (Addgene, #71236) along with a P2A-TagBFP insert into the inducible pTRIPZ backbone harboring an EF1α-driven Tet-On 3G cassette fused to 2A-PuroR. .. The inducible CRISPR knockout construct was generated by subcloning 3xFLAG-Cas9 and IRES-TagBFP into the pCW-Cas9-hPGK-PuroR plasmid (Addgene, #50661). .. Inserts were generated by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L), and subcloning was done by NEBuilder HiFi DNA Assembly (New England Biolabs, E5520L). sgRNA vectors were generated by subcloning mCherry or ZsGreen fused to 2A-BSD (blasticidin resistance gene) downstream of the EF1α promoter in the LentiGuide-Puro vector replacing the PuroR cassette (Addgene, #52963).

    Article Title: PARG inhibition sequesters nuclear PAR-binding proteins, including XRCC1 and its partners, into nuclear condensates to elicit cytotoxicity
    Article Snippet: .. Briefly, EμBcl2+ v-abl kinase-immortalized murine B cell line was infected with lentivirus encoding doxycycline-inducible SpCas9 (Addgene Plasmid #50661). .. A single clone was selected for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 μg/ml for 3 days) and expanded.

    Article Title: Microhomology-mediated end joining acts directly on replication forks to repair single-ended double-strand breaks.
    Article Snippet: In brief Li et al. uncover a new mechanism, forkMMEJ, at broken replication forks that repairs seDSBs with characteristics distinct from the canonical MMEJ (cMMEJ).. This study provides insights into replication-associated DSB repair, implicates microhomology-mediated chromosomal rearrangements in cancer, and offers potential therapeutic strategies to selectively target cancer cells.. ATR Suppress Broken forks

    Infection:

    Article Title: PARG inhibition sequesters nuclear PAR-binding proteins, including XRCC1 and its partners, into nuclear condensates to elicit cytotoxicity
    Article Snippet: .. Briefly, EμBcl2+ v-abl kinase-immortalized murine B cell line was infected with lentivirus encoding doxycycline-inducible SpCas9 (Addgene Plasmid #50661). .. A single clone was selected for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 μg/ml for 3 days) and expanded.

    Software:

    Article Title: Microhomology-mediated end joining acts directly on replication forks to repair single-ended double-strand breaks.
    Article Snippet: In brief Li et al. uncover a new mechanism, forkMMEJ, at broken replication forks that repairs seDSBs with characteristics distinct from the canonical MMEJ (cMMEJ).. This study provides insights into replication-associated DSB repair, implicates microhomology-mediated chromosomal rearrangements in cancer, and offers potential therapeutic strategies to selectively target cancer cells.. ATR Suppress Broken forks



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    Image Search Results


    Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 2 The modified 3TC scaffold boosts SpCas9 gRNA expression levels compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Dunnett’s multiple comparisons test was performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2 × 4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log2RQ ± 95% CI; n = 3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Modification, Expressing, Sequencing, Quantitative Proteomics, Quantitative RT-PCR, Comparison

    Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Journal: BMC genomics

    Article Title: Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction.

    doi: 10.1186/s12864-025-11317-2

    Figure Lengend Snippet: Fig. 5 Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids de livered by lipofection at a (A) high and (B) low plasmid dose without puro mycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n = 3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two- way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001

    Article Snippet: SaCas9Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

    Techniques: Comparison, Plasmid Preparation, Selection, Amplification, Sequencing