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sequencing  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co sequencing
    Sequencing, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capture+sequence/VAHTS+mRNA+Capture+Beads+2%2E0/pm42056557-86-52-49
    Average 96 stars, based on 281 article reviews
    sequencing - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: KDM4B/DHX9 promotes chemoresistance in small-cell lung cancer through the MYCN-driven signaling pathway.
    Article Snippet: Small cell lung cancer (SCLC) is prone to developing chemoresistance, which is associated with epigenetic reprogramming.. While LSD1-mediated histone demethylation has been reported, a systematic investigation into the role of histone demethylases in SCLC chemoresistance is lacking.. In this study, we established nine chemoresistant cell lines from parental cells via gradual dose escalation and conducted RNA sequencing, followed by linear regression and random effects meta-analysis to identify genes associated with chemoresistance among 23 histone demethylases.

    Sequencing:

    Article Title: KDM4B/DHX9 promotes chemoresistance in small-cell lung cancer through the MYCN-driven signaling pathway.
    Article Snippet: Small cell lung cancer (SCLC) is prone to developing chemoresistance, which is associated with epigenetic reprogramming.. While LSD1-mediated histone demethylation has been reported, a systematic investigation into the role of histone demethylases in SCLC chemoresistance is lacking.. In this study, we established nine chemoresistant cell lines from parental cells via gradual dose escalation and conducted RNA sequencing, followed by linear regression and random effects meta-analysis to identify genes associated with chemoresistance among 23 histone demethylases.

    Construct:

    Article Title: KDM4B/DHX9 promotes chemoresistance in small-cell lung cancer through the MYCN-driven signaling pathway.
    Article Snippet: Small cell lung cancer (SCLC) is prone to developing chemoresistance, which is associated with epigenetic reprogramming.. While LSD1-mediated histone demethylation has been reported, a systematic investigation into the role of histone demethylases in SCLC chemoresistance is lacking.. In this study, we established nine chemoresistant cell lines from parental cells via gradual dose escalation and conducted RNA sequencing, followed by linear regression and random effects meta-analysis to identify genes associated with chemoresistance among 23 histone demethylases.

    RNA Sequencing:

    Article Title: KDM4B/DHX9 promotes chemoresistance in small-cell lung cancer through the MYCN-driven signaling pathway.
    Article Snippet: Small cell lung cancer (SCLC) is prone to developing chemoresistance, which is associated with epigenetic reprogramming.. While LSD1-mediated histone demethylation has been reported, a systematic investigation into the role of histone demethylases in SCLC chemoresistance is lacking.. In this study, we established nine chemoresistant cell lines from parental cells via gradual dose escalation and conducted RNA sequencing, followed by linear regression and random effects meta-analysis to identify genes associated with chemoresistance among 23 histone demethylases.

    Article Title: ST3GAL1-associated α-2,3 sialylation supports chondrocyte and osteoblast differentiation during bone development
    Article Snippet: Total RNA was extracted from cultured cells, and RNA quality was assessed using a NanoDrop spectrophotometer, Qubit fluorometer, and Agilent 2100 Bioanalyzer. .. Strand-specific mRNA libraries were prepared using the VAHTS Universal V10 RNA-seq Library Prep Kit with VAHTS mRNA Capture Beads 2.0 (Vazyme) and sequenced on the Illumina NovaSeq 6000 platform in paired-end 150-bp mode. ..

    Purification:

    Article Title: Harnessing GhGRDP1 natural variation for enhanced cotton seed yield: a rhamnose-dependent strategy in breeding
    Article Snippet: Assay preparation and PCR amplifications were performed according to the KASP assay manufacturer’s user guide and manual (LGC Genomics, Shanghai, China). .. Total RNA was purified and eluted using the VAHTS mRNA Capture Beads 2.0 (N403-01, Vazyme). .. Total RNA was purified and eluted using the VAHTS mRNA Capture Beads 2.0 (N403-01, Vazyme).

    Article Title: CircRNA SLAIN1 is associated with reduced malignancy progression of glioblastoma and the M2-like polarization of tumor-associated macrophages via miR-1225-3p/NLRX1 axis.
    Article Snippet: Glioblastoma (GBM) is characterized by the rapid growth of cancer cells, aggressive cell invasion, and abundant immunosuppressive tumor-associated microglia/ macrophages (TAM) in the tumor microenvironment.. Circular RNAs (circRNAs) play a crucial role in various cancers and are significantly associated with tumorigenesis and progression.. However, the involvement of the circRNAs in GBM are still obscure.

    Magnetic Beads:

    Article Title: Six3 promoting the migration and neural differentiation of BMSCs via Btg2‑mediated Akt signaling pathway activation: A therapeutic strategy for stroke.
    Article Snippet: .. Total RNA was extracted using the TRNzol universal reagent (cat. no. DP424, Tiangen Biotech co., Ltd.), and mRNA was enriched from total RNA using Oligo (dT) magnetic beads (cat. no. N403‐01; Vazyme Biotech co., Ltd.), and then fragmented in fragmentation buffers. ..



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    Image Search Results


    (A) Mutation profiles from targeted capture sequencing of injected muscle tissues (S18–S19) and tumors (S1–S17), including three tumors with additional Pten mutations (S9, S13, S14). (B) Top 10 most frequently mutated genes across all tumors.

    Journal: bioRxiv

    Article Title: A tailored in vivo CRISPR screen identifies BAP1 as a potent tumor suppressor of soft tissue sarcoma

    doi: 10.64898/2025.12.01.691734

    Figure Lengend Snippet: (A) Mutation profiles from targeted capture sequencing of injected muscle tissues (S18–S19) and tumors (S1–S17), including three tumors with additional Pten mutations (S9, S13, S14). (B) Top 10 most frequently mutated genes across all tumors.

    Article Snippet: The capture sequencing was done in Azenta.

    Techniques: Mutagenesis, Sequencing, Injection