antibodies against caprin2 nbp1-88318 (Novus Biologicals)
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Novus Biologicals
antibodies against caprin2 nbp1-88318
Antibodies Against Caprin2 Nbp1 88318, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caprin2/chicken+anti+gfap+nbp1+05198/pmc09582274-78-0-5
Average 90 stars, based on 1 article reviews
Antibodies Against Caprin2 Nbp1 88318, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caprin2/chicken+anti+gfap+nbp1+05198/pmc09582274-78-0-5
Average 90 stars, based on 1 article reviews
antibodies against caprin2 nbp1-88318 - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR"
Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR
Journal: Frontiers in Oncology
doi: 10.3389/fonc.2022.931749
Figure Legend Snippet: Effects of CAPRIN2 on the ferroptosis and survival of ECM-detached NPC cells and the migration and invasion of NPC cells. (A) Viability assay of ECM-detached NPC cell lines treated with erastin (5 μM) and/or ferrostatin-1 (1 μM) for 24 h (B) Viability assay of the indicated stable 5-8F or C666-1 cell lines cultured under ECM detachment conditions for 72 h For (A, B) , the experiments were repeated three times, and the data are shown as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. (C, D) Migration and invasion assays of the indicated stable 5-8F (C) and C666-1 (D) cell lines. These assays were conducted in triplicate. Representative images are displayed. The data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Migration, Viability Assay, Cell Culture
Figure Legend Snippet: CAPRIN2 promotes the ferroptosis resistance, survival, migration and invasion of NPC cells through HMGCR. (A) Overexpression of HMGCR partially reverses the effects of CAPRIN2 on the ferroptosis of ECM-detached 5-8F (left panel) and C666-1 (right panel) cells. The NPC cell lines were treated with erastin (5 μM) for 24 h (B, C) MDA assay (B) and GSH assay (C) results of erastin-treated NPC stable cell lines as indicated. (D) Ectopic expression of HMGCR partially rescues the effects of CAPRIN2 knockdown on ECM-detached NPC cell survival. For (A–C) and (D) , the experiments were conducted in triplicate, and the data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01. (E, F) Stable overexpression of HMGCR partially reverses the effects of CAPRIN2 knockdown on 5-8F (E) and C666-1 (F) cell migration and invasion. Representative images of three independent experiments are shown. The data are expressed as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Migration, Over Expression, Multiple Displacement Amplification, GSH Assay, Stable Transfection, Expressing, Knockdown
Figure Legend Snippet: Regulation of lung colonization capacity via the CAPRIN2/HMGCR axis in NPC cells. (A, B) The inhibitory effect of erastin on the lung metastasis of NPC cells was enhanced by knockdown of the CAPRIN2/HMGCR axis. (C, D) CAPRIN2 promotes the lung colonization of NPC cells through HMGCR. For (A, C) , representative images of lungs and HE staining are shown. The location of lung metastatic nodules is indicated by the arrow. For (B, D) , the number of lung metastases (left panel) and the weight of the lungs (right panel) are given. * p < 0.05, ** p < 0.01.
Techniques Used: Knockdown, Staining
Figure Legend Snippet: LINC00941 acts as an upstream molecule to regulate the biological functions of CAPRIN2. (A) LINC00941 downregulation promoted the ferroptosis of ECM-detached NPC cells, which was partially rescued by CAPRIN2 overexpression. The NPC cells were treated with erastin (5 μM) for 24 h (B) Knockdown of LINC00941 decreased the survival of ECM-detached NPC cells, which could be partially reversed by CAPRIN2 overexpression. For (A) and (B) , the assays were conducted in triplicate, and the data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01. (C, D) LINC00941 knockdown inhibited the migration and invasion of 5-8F (C) and C666-1 (D) cells, and overexpression of CAPRIN2 partially reversed this effect. Representative images are shown. The data are provided as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Over Expression, Knockdown, Migration
Figure Legend Snippet: CAPRIN2 is overactivated in NPC tissues and is associated with a poor prognosis in patients. (A) The expression level of CAPRIN2 in 20 NPC tissues and 5 nasopharyngeal tissues. (B) The correlation between LINC00941/CAPRIN2, CAPRIN2/HMGCR or LINC00941/HMGCR in 20 NPC tissues. For (A) and (B) , the expression levels of CAPRIN2, HMGCR and LINC00941 were determined by qRT-PCR. The levels were normalized to those of β-actin and shown as the mean ± SEM. * p < 0.05. (C) Representative immunohistochemical images of normal nasopharyngeal tissues (left panel) and NPC tissues (middle panel). The boxes represent the magnified region. The representative image of negative stained control (right panel, top) shows the negative staining result of NPC tissues incubated with antibody-free serum. The representative image of positive stained control (right panel, bottom) shows the positive staining result of CAPRIN2 in NPC tissues incubated with the primary antibody of CAPRIN2. (D) Kaplan-Meier survival analysis of the association between CAPRIN2 expression and the PFS or OS of NPC patients ( log-rank test ).
Techniques Used: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Staining, Control, Negative Staining, Incubation
Figure Legend Snippet: Correlations between CAPRIN2 expression and clinicopathological characteristics.
Techniques Used: Expressing
Figure Legend Snippet: Univariate and multivariate analysis for OS.
Techniques Used: Expressing
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Staining:Article Title: Sohlh1 Modulates the Stemness and Differentiation of Glioma Stem‐Like Cells by Inactivation of Wnt/β‐Catenin Signalling Pathway via SFRP1 Article Snippet: Primary antibodies are shown as follows: rabbit Article Title: Collagen type VI regulates TGF-β bioavailability in skeletal muscle in mice Article Snippet: The primary antibodies and dilutions were as follows: dystrophin, Sigma-Aldrich D8403 (MANDRA1), mouse IgG1 (1:100 in 5% NGS); collagen VI, Abcam ab6588, rabbit (1:200 in 5% NGS); p-SMAD3, Abcam ab52903, rabbit (1:100 in 10% FBS); LTBP4, R&D Systems AF2885, goat (1:100 in 5% NGS); myosin heavy chain I (MyoHC I), Developmental Studies Hybridoma Bank (DSHB; Iowa City, Iowa, USA) BA-D5, mouse IgG2b (1:20 in 5% NGS); MyoHC IIa, DSHB SC-71, mouse IgG1 (1:20 in 5% NGS); MyoHC IIb, DSHB BF-F3, mouse IgM (1:20 in 5% NGS); periostin, Article Title: Cell‐specific protein expression in Alzheimer's disease prefrontal cortex Article Snippet: Specific cell types were selected using morphology of neurons (neuronal nuclei (NeuN); ABN78, Millipore Sigma, conjugated with Alexa Fluor 647), microglia (Ionized calcium‐binding adapter molecule 1 (Iba1); 48934S, Cell Signaling Technology, conjugated with Alexa Fluor 594), and astrocytes (glial fibrillary acidic protein (GFAP); Article Title: Humanized antibodies binding to amyloid-beta (A-beta) Article Snippet: No or negligible binding was detected for other commercial A-beta antibodies tested (pan-A-beta 6E10, Biolegend) and rabbit anti-A-beta antibodies (D54D2, Cell Signaling; ab201060, (abcam; NBP1-78007, Novus). Article Title: Circular RNA CDYL facilitates hepatocellular carcinoma stemness and PD-L1 + exosomes-mediated immunotherapy resistance via stabilizing hornerin protein by blocking synoviolin 1-mediated ubiquitination. Article Snippet: Despite the revolutionary progress in cancer immunotherapy, only a minority of hepatocellular carcinoma (HCC) patients respond to immune checkpoint inhibitors (ICIs).. In this study, we found that the oncogenic circular RNA Circ-CDYL in HCC influences the efficacy of immunotherapy and the stemness characteristics of HCC cells by interacting with the hornerin (HRNR) protein.. The degraded anti-PD-L1 immunotherapy responses induced by Circ-CDYL and HRNR were confirmed by peripheral blood mononuclear cells (PBMCs) killing assays in HCC cells, patient-derived organoids, and humanized immune system mouse models. Article Title: CYLD-TRAF6 interaction promotes ADP-heptose-induced NF-κB signaling in H. pylori infection. Article Snippet: For the experiments in Fig. 2D–F, 50 ng recombinant human cMyc-FLAG-tagged CYLD (OriGene, SKUTP319629), His-tagged Article Title: Low-dose photodynamic therapy promotes vascular E-selectin expression in chest malignancies, improving immune infiltration and tumor control. Article Snippet: Images were acquired using a Vilber Fusion Fx imaging system. Cross-linking Immunoprecipitation:Article Title: Sohlh1 Modulates the Stemness and Differentiation of Glioma Stem‐Like Cells by Inactivation of Wnt/β‐Catenin Signalling Pathway via SFRP1 Article Snippet: Primary antibodies are shown as follows: rabbit Article Title: Collagen type VI regulates TGF-β bioavailability in skeletal muscle in mice Article Snippet: The primary antibodies and dilutions were as follows: dystrophin, Sigma-Aldrich D8403 (MANDRA1), mouse IgG1 (1:100 in 5% NGS); collagen VI, Abcam ab6588, rabbit (1:200 in 5% NGS); p-SMAD3, Abcam ab52903, rabbit (1:100 in 10% FBS); LTBP4, R&D Systems AF2885, goat (1:100 in 5% NGS); myosin heavy chain I (MyoHC I), Developmental Studies Hybridoma Bank (DSHB; Iowa City, Iowa, USA) BA-D5, mouse IgG2b (1:20 in 5% NGS); MyoHC IIa, DSHB SC-71, mouse IgG1 (1:20 in 5% NGS); MyoHC IIb, DSHB BF-F3, mouse IgM (1:20 in 5% NGS); periostin, Article Title: Cell‐specific protein expression in Alzheimer's disease prefrontal cortex Article Snippet: Specific cell types were selected using morphology of neurons (neuronal nuclei (NeuN); ABN78, Millipore Sigma, conjugated with Alexa Fluor 647), microglia (Ionized calcium‐binding adapter molecule 1 (Iba1); 48934S, Cell Signaling Technology, conjugated with Alexa Fluor 594), and astrocytes (glial fibrillary acidic protein (GFAP); Article Title: Humanized antibodies binding to amyloid-beta (A-beta) Article Snippet: No or negligible binding was detected for other commercial A-beta antibodies tested (pan-A-beta 6E10, Biolegend) and rabbit anti-A-beta antibodies (D54D2, Cell Signaling; ab201060, (abcam; NBP1-78007, Novus). Article Title: Circular RNA CDYL facilitates hepatocellular carcinoma stemness and PD-L1 + exosomes-mediated immunotherapy resistance via stabilizing hornerin protein by blocking synoviolin 1-mediated ubiquitination. Article Snippet: Despite the revolutionary progress in cancer immunotherapy, only a minority of hepatocellular carcinoma (HCC) patients respond to immune checkpoint inhibitors (ICIs).. In this study, we found that the oncogenic circular RNA Circ-CDYL in HCC influences the efficacy of immunotherapy and the stemness characteristics of HCC cells by interacting with the hornerin (HRNR) protein.. The degraded anti-PD-L1 immunotherapy responses induced by Circ-CDYL and HRNR were confirmed by peripheral blood mononuclear cells (PBMCs) killing assays in HCC cells, patient-derived organoids, and humanized immune system mouse models. Article Title: CYLD-TRAF6 interaction promotes ADP-heptose-induced NF-κB signaling in H. pylori infection. 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Labeling:Article Title: Sohlh1 Modulates the Stemness and Differentiation of Glioma Stem‐Like Cells by Inactivation of Wnt/β‐Catenin Signalling Pathway via SFRP1 Article Snippet: Primary antibodies are shown as follows: rabbit Article Title: Collagen type VI regulates TGF-β bioavailability in skeletal muscle in mice Article Snippet: The primary antibodies and dilutions were as follows: dystrophin, Sigma-Aldrich D8403 (MANDRA1), mouse IgG1 (1:100 in 5% NGS); collagen VI, Abcam ab6588, rabbit (1:200 in 5% NGS); p-SMAD3, Abcam ab52903, rabbit (1:100 in 10% FBS); LTBP4, R&D Systems AF2885, goat (1:100 in 5% NGS); myosin heavy chain I (MyoHC I), Developmental Studies Hybridoma Bank (DSHB; Iowa City, Iowa, USA) BA-D5, mouse IgG2b (1:20 in 5% NGS); MyoHC IIa, DSHB SC-71, mouse IgG1 (1:20 in 5% NGS); MyoHC IIb, DSHB BF-F3, mouse IgM (1:20 in 5% NGS); periostin, Article Title: Cell‐specific protein expression in Alzheimer's disease prefrontal cortex Article Snippet: Specific cell types were selected using morphology of neurons (neuronal nuclei (NeuN); ABN78, Millipore Sigma, conjugated with Alexa Fluor 647), microglia (Ionized calcium‐binding adapter molecule 1 (Iba1); 48934S, Cell Signaling Technology, conjugated with Alexa Fluor 594), and astrocytes (glial fibrillary acidic protein (GFAP); Article Title: Humanized antibodies binding to amyloid-beta (A-beta) Article Snippet: No or negligible binding was detected for other commercial A-beta antibodies tested (pan-A-beta 6E10, Biolegend) and rabbit anti-A-beta antibodies (D54D2, Cell Signaling; ab201060, (abcam; NBP1-78007, Novus). Article Title: Circular RNA CDYL facilitates hepatocellular carcinoma stemness and PD-L1 + exosomes-mediated immunotherapy resistance via stabilizing hornerin protein by blocking synoviolin 1-mediated ubiquitination. Article Snippet: Despite the revolutionary progress in cancer immunotherapy, only a minority of hepatocellular carcinoma (HCC) patients respond to immune checkpoint inhibitors (ICIs).. In this study, we found that the oncogenic circular RNA Circ-CDYL in HCC influences the efficacy of immunotherapy and the stemness characteristics of HCC cells by interacting with the hornerin (HRNR) protein.. The degraded anti-PD-L1 immunotherapy responses induced by Circ-CDYL and HRNR were confirmed by peripheral blood mononuclear cells (PBMCs) killing assays in HCC cells, patient-derived organoids, and humanized immune system mouse models. Article Title: CYLD-TRAF6 interaction promotes ADP-heptose-induced NF-κB signaling in H. pylori infection. Article Snippet: For the experiments in Fig. 2D–F, 50 ng recombinant human cMyc-FLAG-tagged CYLD (OriGene, SKUTP319629), His-tagged Article Title: Low-dose photodynamic therapy promotes vascular E-selectin expression in chest malignancies, improving immune infiltration and tumor control. Article Snippet: Images were acquired using a Vilber Fusion Fx imaging system. Fluorescence:Article Title: Sohlh1 Modulates the Stemness and Differentiation of Glioma Stem‐Like Cells by Inactivation of Wnt/β‐Catenin Signalling Pathway via SFRP1 Article Snippet: Primary antibodies are shown as follows: rabbit Article Title: Collagen type VI regulates TGF-β bioavailability in skeletal muscle in mice Article Snippet: The primary antibodies and dilutions were as follows: dystrophin, Sigma-Aldrich D8403 (MANDRA1), mouse IgG1 (1:100 in 5% NGS); collagen VI, Abcam ab6588, rabbit (1:200 in 5% NGS); p-SMAD3, Abcam ab52903, rabbit (1:100 in 10% FBS); LTBP4, R&D Systems AF2885, goat (1:100 in 5% NGS); myosin heavy chain I (MyoHC I), Developmental Studies Hybridoma Bank (DSHB; Iowa City, Iowa, USA) BA-D5, mouse IgG2b (1:20 in 5% NGS); MyoHC IIa, DSHB SC-71, mouse IgG1 (1:20 in 5% NGS); MyoHC IIb, DSHB BF-F3, mouse IgM (1:20 in 5% NGS); periostin, Article Title: Cell‐specific protein expression in Alzheimer's disease prefrontal cortex Article Snippet: Specific cell types were selected using morphology of neurons (neuronal nuclei (NeuN); ABN78, Millipore Sigma, conjugated with Alexa Fluor 647), microglia (Ionized calcium‐binding adapter molecule 1 (Iba1); 48934S, Cell Signaling Technology, conjugated with Alexa Fluor 594), and astrocytes (glial fibrillary acidic protein (GFAP); Article Title: Humanized antibodies binding to amyloid-beta (A-beta) Article Snippet: No or negligible binding was detected for other commercial A-beta antibodies tested (pan-A-beta 6E10, Biolegend) and rabbit anti-A-beta antibodies (D54D2, Cell Signaling; ab201060, (abcam; NBP1-78007, Novus). Article Title: Circular RNA CDYL facilitates hepatocellular carcinoma stemness and PD-L1 + exosomes-mediated immunotherapy resistance via stabilizing hornerin protein by blocking synoviolin 1-mediated ubiquitination. Article Snippet: Despite the revolutionary progress in cancer immunotherapy, only a minority of hepatocellular carcinoma (HCC) patients respond to immune checkpoint inhibitors (ICIs).. In this study, we found that the oncogenic circular RNA Circ-CDYL in HCC influences the efficacy of immunotherapy and the stemness characteristics of HCC cells by interacting with the hornerin (HRNR) protein.. The degraded anti-PD-L1 immunotherapy responses induced by Circ-CDYL and HRNR were confirmed by peripheral blood mononuclear cells (PBMCs) killing assays in HCC cells, patient-derived organoids, and humanized immune system mouse models. Article Title: CYLD-TRAF6 interaction promotes ADP-heptose-induced NF-κB signaling in H. pylori infection. Article Snippet: For the experiments in Fig. 2D–F, 50 ng recombinant human cMyc-FLAG-tagged CYLD (OriGene, SKUTP319629), His-tagged Article Title: Low-dose photodynamic therapy promotes vascular E-selectin expression in chest malignancies, improving immune infiltration and tumor control. Article Snippet: Images were acquired using a Vilber Fusion Fx imaging system. Recombinant:Article Title: Sohlh1 Modulates the Stemness and Differentiation of Glioma Stem‐Like Cells by Inactivation of Wnt/β‐Catenin Signalling Pathway via SFRP1 Article Snippet: Primary antibodies are shown as follows: rabbit Article Title: Collagen type VI regulates TGF-β bioavailability in skeletal muscle in mice Article Snippet: The primary antibodies and dilutions were as follows: dystrophin, Sigma-Aldrich D8403 (MANDRA1), mouse IgG1 (1:100 in 5% NGS); collagen VI, Abcam ab6588, rabbit (1:200 in 5% NGS); p-SMAD3, Abcam ab52903, rabbit (1:100 in 10% FBS); LTBP4, R&D Systems AF2885, goat (1:100 in 5% NGS); myosin heavy chain I (MyoHC I), Developmental Studies Hybridoma Bank (DSHB; Iowa City, Iowa, USA) BA-D5, mouse IgG2b (1:20 in 5% NGS); MyoHC IIa, DSHB SC-71, mouse IgG1 (1:20 in 5% NGS); MyoHC IIb, DSHB BF-F3, mouse IgM (1:20 in 5% NGS); periostin, Article Title: Cell‐specific protein expression in Alzheimer's disease prefrontal cortex Article Snippet: Specific cell types were selected using morphology of neurons (neuronal nuclei (NeuN); ABN78, Millipore Sigma, conjugated with Alexa Fluor 647), microglia (Ionized calcium‐binding adapter molecule 1 (Iba1); 48934S, Cell Signaling Technology, conjugated with Alexa Fluor 594), and astrocytes (glial fibrillary acidic protein (GFAP); Article Title: Humanized antibodies binding to amyloid-beta (A-beta) Article Snippet: No or negligible binding was detected for other commercial A-beta antibodies tested (pan-A-beta 6E10, Biolegend) and rabbit anti-A-beta antibodies (D54D2, Cell Signaling; ab201060, (abcam; NBP1-78007, Novus). Article Title: Circular RNA CDYL facilitates hepatocellular carcinoma stemness and PD-L1 + exosomes-mediated immunotherapy resistance via stabilizing hornerin protein by blocking synoviolin 1-mediated ubiquitination. Article Snippet: Despite the revolutionary progress in cancer immunotherapy, only a minority of hepatocellular carcinoma (HCC) patients respond to immune checkpoint inhibitors (ICIs).. In this study, we found that the oncogenic circular RNA Circ-CDYL in HCC influences the efficacy of immunotherapy and the stemness characteristics of HCC cells by interacting with the hornerin (HRNR) protein.. The degraded anti-PD-L1 immunotherapy responses induced by Circ-CDYL and HRNR were confirmed by peripheral blood mononuclear cells (PBMCs) killing assays in HCC cells, patient-derived organoids, and humanized immune system mouse models. Article Title: CYLD-TRAF6 interaction promotes ADP-heptose-induced NF-κB signaling in H. pylori infection. Article Snippet: For the experiments in Fig. 2D–F, 50 ng recombinant human cMyc-FLAG-tagged CYLD (OriGene, SKUTP319629), His-tagged Article Title: Low-dose photodynamic therapy promotes vascular E-selectin expression in chest malignancies, improving immune infiltration and tumor control. Article Snippet: Images were acquired using a Vilber Fusion Fx imaging system. Next-Generation Sequencing:Article Title: Sohlh1 Modulates the Stemness and Differentiation of Glioma Stem‐Like Cells by Inactivation of Wnt/β‐Catenin Signalling Pathway via SFRP1 Article Snippet: Primary antibodies are shown as follows: rabbit Article Title: Collagen type VI regulates TGF-β bioavailability in skeletal muscle in mice Article Snippet: The primary antibodies and dilutions were as follows: dystrophin, Sigma-Aldrich D8403 (MANDRA1), mouse IgG1 (1:100 in 5% NGS); collagen VI, Abcam ab6588, rabbit (1:200 in 5% NGS); p-SMAD3, Abcam ab52903, rabbit (1:100 in 10% FBS); LTBP4, R&D Systems AF2885, goat (1:100 in 5% NGS); myosin heavy chain I (MyoHC I), Developmental Studies Hybridoma Bank (DSHB; Iowa City, Iowa, USA) BA-D5, mouse IgG2b (1:20 in 5% NGS); MyoHC IIa, DSHB SC-71, mouse IgG1 (1:20 in 5% NGS); MyoHC IIb, DSHB BF-F3, mouse IgM (1:20 in 5% NGS); periostin, Article Title: Cell‐specific protein expression in Alzheimer's disease prefrontal cortex Article Snippet: Specific cell types were selected using morphology of neurons (neuronal nuclei (NeuN); ABN78, Millipore Sigma, conjugated with Alexa Fluor 647), microglia (Ionized calcium‐binding adapter molecule 1 (Iba1); 48934S, Cell Signaling Technology, conjugated with Alexa Fluor 594), and astrocytes (glial fibrillary acidic protein (GFAP); Article Title: Humanized antibodies binding to amyloid-beta (A-beta) Article Snippet: No or negligible binding was detected for other commercial A-beta antibodies tested (pan-A-beta 6E10, Biolegend) and rabbit anti-A-beta antibodies (D54D2, Cell Signaling; ab201060, (abcam; NBP1-78007, Novus). Article Title: Circular RNA CDYL facilitates hepatocellular carcinoma stemness and PD-L1 + exosomes-mediated immunotherapy resistance via stabilizing hornerin protein by blocking synoviolin 1-mediated ubiquitination. Article Snippet: Despite the revolutionary progress in cancer immunotherapy, only a minority of hepatocellular carcinoma (HCC) patients respond to immune checkpoint inhibitors (ICIs).. In this study, we found that the oncogenic circular RNA Circ-CDYL in HCC influences the efficacy of immunotherapy and the stemness characteristics of HCC cells by interacting with the hornerin (HRNR) protein.. The degraded anti-PD-L1 immunotherapy responses induced by Circ-CDYL and HRNR were confirmed by peripheral blood mononuclear cells (PBMCs) killing assays in HCC cells, patient-derived organoids, and humanized immune system mouse models. Article Title: CYLD-TRAF6 interaction promotes ADP-heptose-induced NF-κB signaling in H. pylori infection. Article Snippet: For the experiments in Fig. 2D–F, 50 ng recombinant human cMyc-FLAG-tagged CYLD (OriGene, SKUTP319629), His-tagged Article Title: Low-dose photodynamic therapy promotes vascular E-selectin expression in chest malignancies, improving immune infiltration and tumor control. Article Snippet: Images were acquired using a Vilber Fusion Fx imaging system. Western Blot:Article Title: Sohlh1 Modulates the Stemness and Differentiation of Glioma Stem‐Like Cells by Inactivation of Wnt/β‐Catenin Signalling Pathway via SFRP1 Article Snippet: Primary antibodies are shown as follows: rabbit Article Title: Collagen type VI regulates TGF-β bioavailability in skeletal muscle in mice Article Snippet: The primary antibodies and dilutions were as follows: dystrophin, Sigma-Aldrich D8403 (MANDRA1), mouse IgG1 (1:100 in 5% NGS); collagen VI, Abcam ab6588, rabbit (1:200 in 5% NGS); p-SMAD3, Abcam ab52903, rabbit (1:100 in 10% FBS); LTBP4, R&D Systems AF2885, goat (1:100 in 5% NGS); myosin heavy chain I (MyoHC I), Developmental Studies Hybridoma Bank (DSHB; Iowa City, Iowa, USA) BA-D5, mouse IgG2b (1:20 in 5% NGS); MyoHC IIa, DSHB SC-71, mouse IgG1 (1:20 in 5% NGS); MyoHC IIb, DSHB BF-F3, mouse IgM (1:20 in 5% NGS); periostin, Article Title: Cell‐specific protein expression in Alzheimer's disease prefrontal cortex Article Snippet: Specific cell types were selected using morphology of neurons (neuronal nuclei (NeuN); ABN78, Millipore Sigma, conjugated with Alexa Fluor 647), microglia (Ionized calcium‐binding adapter molecule 1 (Iba1); 48934S, Cell Signaling Technology, conjugated with Alexa Fluor 594), and astrocytes (glial fibrillary acidic protein (GFAP); Article Title: Humanized antibodies binding to amyloid-beta (A-beta) Article Snippet: No or negligible binding was detected for other commercial A-beta antibodies tested (pan-A-beta 6E10, Biolegend) and rabbit anti-A-beta antibodies (D54D2, Cell Signaling; ab201060, (abcam; NBP1-78007, Novus). Article Title: Circular RNA CDYL facilitates hepatocellular carcinoma stemness and PD-L1 + exosomes-mediated immunotherapy resistance via stabilizing hornerin protein by blocking synoviolin 1-mediated ubiquitination. Article Snippet: Despite the revolutionary progress in cancer immunotherapy, only a minority of hepatocellular carcinoma (HCC) patients respond to immune checkpoint inhibitors (ICIs).. In this study, we found that the oncogenic circular RNA Circ-CDYL in HCC influences the efficacy of immunotherapy and the stemness characteristics of HCC cells by interacting with the hornerin (HRNR) protein.. The degraded anti-PD-L1 immunotherapy responses induced by Circ-CDYL and HRNR were confirmed by peripheral blood mononuclear cells (PBMCs) killing assays in HCC cells, patient-derived organoids, and humanized immune system mouse models. Article Title: CYLD-TRAF6 interaction promotes ADP-heptose-induced NF-κB signaling in H. pylori infection. Article Snippet: For the experiments in Fig. 2D–F, 50 ng recombinant human cMyc-FLAG-tagged CYLD (OriGene, SKUTP319629), His-tagged Article Title: Low-dose photodynamic therapy promotes vascular E-selectin expression in chest malignancies, improving immune infiltration and tumor control. Article Snippet: Images were acquired using a Vilber Fusion Fx imaging system. |
